肺痨康对COPD模型大鼠脏器系数的影响
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摘要
目的:观察肺痨康对慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)模型大鼠脏器系数的影响,探究其对COPD模型大鼠是否具有治疗作用及其作用机制。方法:将60只SD雄性大鼠适应性喂养1周后随机分为空白组、模型组、地米塞松组及肺痨康高、中、低剂量组,采用香烟烟熏(CS)+脂多糖(LPS)气管内滴注复合因素法建立COPD大鼠模型,于造模第15天开始,肺痨康低、中、高剂量组分别予肺痨康药液0. 1405、0. 281、0. 843ml/(kg·d)灌胃,地塞米松组予地塞米松每天每只0. 054mg灌胃,空白组、模型组予相应体积0. 9%氯化钠注射液灌胃,每天1次。灌胃14d后处死、取材、称重并计算相关脏器系数。结果:与模型组比较,地塞米松组、肺痨康高剂量组能不同程度地降低肺脏系数(P <0. 01),提高脾脏系数和胸腺系数(P <0. 01,P <0. 05),肺痨康中、低剂量组能提高胸腺系数(P <0. 01)。结论:肺痨康高剂量可减轻模型大鼠肺脏的炎症反应并增强其免疫力。
Objective: To investigate the effect of Feilaokang on organ coefficient in rats with chronic obstructive pulmonary disease( COPD),as well as its therapeutic effect on COPD and possible mechanism of action. Methods: After one week of adaptive feeding,60 Sprague-Dawley rats were randomly divided into blank group,model group,dexamethasone group,and high-,middle-,and low-dose Feilaokang groups. A rat model of COPD was established by cigarette smoke combined with intratracheal instillation of lipopolysaccharide. From day 15 of modeling,the rats in the low-,middle-,and high-dose Feilaokang groups were given Feilaokang drug solution once a day by gavage at a dose of 0. 1405,0. 281,and 0. 843 ml/kg/d,respectively,each rat in the dexamethasone group was given dexamethasone once a day by gavage at a dose of 0. 054 mg/d,and those in the blank group and the model group were given an equal volume of 0. 9% sodium chloride injection once a day by gavage. The rats were sacrificed after 14 days of treatment by gavage,and related samples were collected and weighed to calculate the organ coefficient. Results: Compared with the model group,the dexamethasone group and the high-dose Feilaokang group had a significant reduction in lung coefficient( P < 0. 01) and significant increases in spleen coefficient and thymus index( P < 0. 01 or P < 0. 05),and the middle-and low-dose Feilaokang groups had a significant increase in thymus index( P < 0. 01). Conclusion: High-dose Feilaokang can alleviate lung inflammatory response and enhance immunity in rats with COPD.
Objective: To investigate the effect of Feilaokang on organ coefficient in rats with chronic obstructive pulmonary disease( COPD),as well as its therapeutic effect on COPD and possible mechanism of action. Methods: After one week of adaptive feeding,60 Sprague-Dawley rats were randomly divided into blank group,model group,dexamethasone group,and high-,middle-,and low-dose Feilaokang groups. A rat model of COPD was established by cigarette smoke combined with intratracheal instillation of lipopolysaccharide. From day 15 of modeling,the rats in the low-,middle-,and high-dose Feilaokang groups were given Feilaokang drug solution once a day by gavage at a dose of 0. 1405,0. 281,and 0. 843 ml/kg/d,respectively,each rat in the dexamethasone group was given dexamethasone once a day by gavage at a dose of 0. 054 mg/d,and those in the blank group and the model group were given an equal volume of 0. 9% sodium chloride injection once a day by gavage. The rats were sacrificed after 14 days of treatment by gavage,and related samples were collected and weighed to calculate the organ coefficient. Results: Compared with the model group,the dexamethasone group and the high-dose Feilaokang group had a significant reduction in lung coefficient( P < 0. 01) and significant increases in spleen coefficient and thymus index( P < 0. 01 or P < 0. 05),and the middle-and low-dose Feilaokang groups had a significant increase in thymus index( P < 0. 01). Conclusion: High-dose Feilaokang can alleviate lung inflammatory response and enhance immunity in rats with COPD.
引文
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[6]黄修解,蒙定水,牛豫洁,等.双三口服液对实验COPD大鼠血清白细胞介素-8和肿瘤坏死因子-a的影响[J].中国中医基础医学杂志,2010,16(5):381-383.
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[2]宋一平,崔德健,茅培英.慢性阻塞性肺病大鼠模型的建立及药物干预的影响[J].军医进修学院报,2001,22(2):99-102.
[3]邹海峰,赵春玲,陈燕,等.慢性阻塞性肺疾病大鼠肺牵张反射对心率的影响[J].西部医学,2007,19(1):9-11.
[4]金众,庞宝森,武维屏,等.一种实验性大鼠慢性阻塞性肺疾病模型的建立[J].心肺血管病杂志,2004,23(3):179-181.
[5]柏正平,谭光波,胡学军,等.通肺净痰方对COPD痰热证免疫功能的影响[J].中华中医药学刊,2010,28(12):2674-2676.
[6]黄修解,蒙定水,牛豫洁,等.双三口服液对实验COPD大鼠血清白细胞介素-8和肿瘤坏死因子-a的影响[J].中国中医基础医学杂志,2010,16(5):381-383.
[7]申金贵,叶品良,王帅,等.肺痨康对小鼠结核病模型的免疫指标观察[J].浙江中医药大学学报,2010,34(1):48-49.