醋酸氟孕酮载药系统的初步研究
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摘要
采用化学共沉淀法制备Fe_3O_4纳米粒子(MNPs),对Fe_3O_4MNPs进行无机材料SiO_2包被和氨基化,依次得到Fe_3O_4@SiO_2和Fe_3O_4@SiO_2-NH_2,再对Fe_3O_4@SiO_2-NH_2和醋酸氟孕酮(FGA)进行PEG化,分别得到Fe_3O_4@SiO_2-NH_2-PEG和PEG-FGA产物.分别以20 mg∶20 mg、20 mg∶10 mg的比例进行Fe_3O_4@SiO_2-NH_2-PEG和PEG-FGA的装载研究.最后利用紫外-可见分光光度法对样品装载、释放的结果进行吸光度测定,通过FGA的浓度标准曲线,计算样品的释放浓度.结果表现为在释放过程中,从4-20 d数据结果看,20 mg∶10 mg的样品要好于20 mg∶20 mg的样品,所以确定20 mg∶10 mg用作药物释放研究的浓度.
Fe_3O_4(MNPs) was prepared by chemical co-precipitation method. Fe_3O_4(MNPs) was coated with inorganic material silica, followed by amination, and Fe_3O_4@SiO_2 and Fe_3O_4@SiO_2-NH_2 were obtained.PEGylation of Fe_3O_4@SiO_2-NH_2 and fluocin progesterone acetate(FGA) resulted in Fe_3O_4@SiO_2-NH_2-PEG and PEG-FGA products, respectively. In this experiment, the loading studies of Fe_3O_4@SiO_2-NH_2-PEG and PEG-FGA were carried out at a ratio of 20 mg:20 mg, 20 mg:10 mg respectively. Finally, the absorbance of the loaded and released samples was determined by UV-visible spectrophotometry. Therefore,the concentration of the released samples was calculated by the standard curve of FGA. The results showed that during the releasing process, the absorbance of the sample(20 mg:10 mg) at 20 d was better than that of the sample(20 mg:20 mg) at 4 d, so the concentration of 20 mg:10 mg was determined to be sufficient for the release.
Fe_3O_4(MNPs) was prepared by chemical co-precipitation method. Fe_3O_4(MNPs) was coated with inorganic material silica, followed by amination, and Fe_3O_4@SiO_2 and Fe_3O_4@SiO_2-NH_2 were obtained.PEGylation of Fe_3O_4@SiO_2-NH_2 and fluocin progesterone acetate(FGA) resulted in Fe_3O_4@SiO_2-NH_2-PEG and PEG-FGA products, respectively. In this experiment, the loading studies of Fe_3O_4@SiO_2-NH_2-PEG and PEG-FGA were carried out at a ratio of 20 mg:20 mg, 20 mg:10 mg respectively. Finally, the absorbance of the loaded and released samples was determined by UV-visible spectrophotometry. Therefore,the concentration of the released samples was calculated by the standard curve of FGA. The results showed that during the releasing process, the absorbance of the sample(20 mg:10 mg) at 20 d was better than that of the sample(20 mg:20 mg) at 4 d, so the concentration of 20 mg:10 mg was determined to be sufficient for the release.
引文
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13 Beamson G. Conformation and orientation effects in the X-ray photoelectron spectra of organic polymers[J]. Journal of Electron Spectroscopy and Related Phenomena, 2001, 121:163-181.
14 El-Ashgar N M, El-Nahhal I M, Chehimi M M, et al. Synthesis and structural characterization of a new macrocyclic polysiloxane-immobilized ligand system[J]. J Monatsh Chem, 2006, 137:263-275.
15 Siddiquey I A, Ukaji E, Furusawa T, et al. The effects of organic surface treatment by methacryloxypropyltrimethoxysilane on the photostability of TiO2[J]. Materials Chemistry and Physics, 2007, 105:162-168.
2 Lu A H, Salabas E L, Schüth F. Magnetic nanoparticles:Synthesis, protection, functionalization, and application[J]. Angew Chem Int Ed, 2007, 46:1 222-1 244.
3 杨咏来,宁桂玲,吕秉玲.液相法制备纳米粉体时防团聚方法概述[J].材料导报,1998,12(2):11-13.
4 高濂,孙静,刘阳桥.纳米粉体的分散及表面改性[M].北京:化学工业出版社, 2004:207.
5 Qiu G M, Zhu B K, Xu Y Y.α-Amylase immobilized by Fe3O4/poly-(strene-co-maleicanhydride)magnetic composite microspheres:preparation and characterization[J]. Journal of Applied Polymer Science, 2005, 95(2):328-335.
6 冯辉霞,刘静晨,张德懿,等.超顺磁性纳米Fe3O4的制备、表面改性方法与应用研究[J]. 2011, 40(7):1 248-1 253.
7 Lu H, Yi G, Zhao S, et al. Synthesis and characterization of multi-functional nanoparticles possessing magnetic,up-conversion fluorescence and bio-affinity properties[J]. Journal of Materials Chemistry, 2004, 14:1 336-1 341.
8 张宝良,张秋禹,张和鹏,等.单分散Fe3O4纳米粒子的制备与改性的研究进展[J].材料导报, 2009, 23(4):112-116.
9 刘骏.沉淀氧化法制备Fe3O4磁流体[J].武汉工业学院学报, 2009, 28(2):42-44.
10 Shen L, Laibinis P E, Hatton T A. Bilayer surfactant stabilized magnetic fluids:Synthesis and interactions at interfaces[J]. Langmuir, 1999, 15:447-453.
11 St?ber W, Fink A. Controlled growth of monodisperse silica spheres in the micron size range[J]. J Colloid and Interface Science, 1968, 26:62-69.
12 何晓红,杨利国.阴道海绵栓“羊乐”中醋酸氟孕酮体外释放规律研究[J].南京农业大学学报, 2002, 25(2):75-78.
13 Beamson G. Conformation and orientation effects in the X-ray photoelectron spectra of organic polymers[J]. Journal of Electron Spectroscopy and Related Phenomena, 2001, 121:163-181.
14 El-Ashgar N M, El-Nahhal I M, Chehimi M M, et al. Synthesis and structural characterization of a new macrocyclic polysiloxane-immobilized ligand system[J]. J Monatsh Chem, 2006, 137:263-275.
15 Siddiquey I A, Ukaji E, Furusawa T, et al. The effects of organic surface treatment by methacryloxypropyltrimethoxysilane on the photostability of TiO2[J]. Materials Chemistry and Physics, 2007, 105:162-168.