黄花棘豆对小鼠脏器中SOD、MDA、GSH-Px水平的影响
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摘要
探讨棘豆对小鼠体内氧自由基代谢的影响。将40只小鼠分为2组,分别灌服黄花棘豆水煎液和生理盐水0.25 m L/只,连灌40 d。末次灌胃后断头处死,取出有关组织脏器,测定SOD、MDA、GSH-Px等指标。结果表明,与对照组小鼠相比,试验组小鼠心脏中的SOD含量显著升高(P<0.05),其他组织中SOD活性差异不显著;心脏、肾脏中的MDA含量均显著降低(P<0.05),其他组织中MDA含量差异不显著;心脏、肾脏中的GSH-Px活性均显著升高(P<0.05),其他组织中GSH-Px活性差异不显著。说明棘豆对小鼠具有一定的抗氧化、抗衰老作用。
In order to observe the effects of Oxytropis ochrocephala on the activity and contents of SOD,GSH-Px and MDA in mice viscera,40 mice were divided into 2 groups. 1% O.ochrocephala decoction and saline were administrated by lavage to each for continuous 40 d,respectively. Mice were decapitated and heart,liver,spleen,lung,and kidney were removed after the last administration. The contents of SOD,GSH-Px,and MDA in heart,liver,spleen,lung and kidney were determined. Results show that O.ochrocephala could increase the activity of SOD significantly(P<0.05) in heart. The contents of MDA were significantly lower(P<0.05)in heart and kidney; the activity of GSH-Px was significantly increased in heart and kidney. So O.ochrocephala can improve the anti-oxidation ability in tissues of mice.
In order to observe the effects of Oxytropis ochrocephala on the activity and contents of SOD,GSH-Px and MDA in mice viscera,40 mice were divided into 2 groups. 1% O.ochrocephala decoction and saline were administrated by lavage to each for continuous 40 d,respectively. Mice were decapitated and heart,liver,spleen,lung,and kidney were removed after the last administration. The contents of SOD,GSH-Px,and MDA in heart,liver,spleen,lung and kidney were determined. Results show that O.ochrocephala could increase the activity of SOD significantly(P<0.05) in heart. The contents of MDA were significantly lower(P<0.05)in heart and kidney; the activity of GSH-Px was significantly increased in heart and kidney. So O.ochrocephala can improve the anti-oxidation ability in tissues of mice.
引文
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[11]Novikoff P M,Touster O,Novikoff A B,et al.Effects of swainsonine on rat liver and kidney:Biochemical and morphological studies[J].Journal of Cell Biology,1985,101(2):339-349.
[2]杨具田,王英东,李建科,等.甘肃棘豆草粉对肥育猪的效果和毒性试验[J].中国兽医科技,1997,27(3):55.
[3]李建科.棘豆化学防除研究Ⅱ[J].西北民族学院学报(自然科学版),1992,13(1):38-40.
[4]陈奇.中药药理研究方法学[M].北京:人民卫生出版社,1993.
[5]杨欢,王栋,童丽,等.镰形棘豆的化学成分研究(Ⅰ)[J].中国药学杂志,2008,43(20):1538-1540.
[6]王凯.棘豆的危害及研究进展[J].动物毒物学,1994,3(1):33-35.
[7]王明臣,张济敏,苗健,等.血清超氧化物歧化酶同工酶活性与衰老关系[J].河南医科大学学报,1992,27(2):144-145.
[8]高新磊,韩冰,赵萌莉,等.疯草及毒性成分研究进展[J].草业学报,2011,20(3):279-286.
[9]王栋,杨欢,童丽,等.藏药镰形棘豆的镇痛抗炎活性[J].药学与临床研究,2008,16(2):90-93.
[10]戴衍朋,杨欢,童丽,等.镰形棘豆止血作用初步研究[J].南京中医药大学学报(自然科学版),2008,24(2):107-108,126.
[11]Novikoff P M,Touster O,Novikoff A B,et al.Effects of swainsonine on rat liver and kidney:Biochemical and morphological studies[J].Journal of Cell Biology,1985,101(2):339-349.