一株异养硝化细菌的筛选鉴定及硝化性能研究
摘要
本研究从位于杭州某化肥厂污水池中筛选出一株异养硝化细菌。根据形态学特征及16SrDNA序列分析,初步判定该菌株为恶臭假单胞菌,命名为DF-1。通过单因素试验研究DF-1的生长和脱氮特性,结果表明以柠檬酸钠为碳源、硫酸铵为氮源时,菌株DF-1在20℃、60r/min、C/N为14条件下,培养24h后氨氮去除率达到99. 8%,总氮去除率为97. 8%。整个硝化过程中没有硝态氮和亚硝态氮积累,p H由7. 0增加到8. 8。通过反硝化能力验证试验,发现菌株不能以亚硝态氮或硝态氮为唯一氮源生长。菌株DF-1具有良好的耐高氨氮特性,在初始氨氮浓度为1000mg/L的条件下培养36h后,氨氮去除率达到52. 7%。表明菌株DF-1在高氨氮废水的生物处理方面具有良好的应用前景。
引文
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[3] Robertson L A,Kuenen J G. Thiosphaera pantotropha gen. nov. sp.nov.,a facultatively anaerobic,facultatively autotrophic sulphur bacterium[J]. Microbiology,1983,129(9):2847-2855.
[4]罗胜南,尚润东,靳永胜.我国微生物法去除氨氮研究进展[J].生物技术进展,2017(2).
[5] Li C,Yang J,Wang X,et al. Removal of nitrogen by heterotrophic nitrification–aerobic denitrification of a phosphate accumulating bacterium Pseudomonas stutzeri YG-24[J]. Bioresource Technology,2015,182:18-25.
[6] Qing H,Donde O O,Tian C,et al. Novel heterotrophic nitrogen removal and assimilation characteristic of the newly isolated bacterium,Pseudomonas stutzeri,AD-1[J]. Journal of Bioscience and Bioengineering,2018:S1389172317310800.
[7] Huang F,Pan L,Lv N,et al. Characterization of novel Bacillus strain N31 from mariculture water capable of halophilic heterotrophic nitrification-aerobic denitrification[J]. Journal of Bioscience&Bioengineering,2017,124(5).
[8] Nitrogen removal characteristics of a heterotrophic nitrifier Acinetobacter junii YB and its potential application for the treatment of high-strength nitrogenous wastewater[J]. Bioresource Technology,2015,193:227-233.
[9] Padhi S K,Tripathy S,Mohanty S,et al. Aerobic and heterotrophic nitrogen removal by,Enterobacter cloacae,CF-S27 with efficient utilization of hydroxylamine[J]. Bioresource Technology,2017,232(Complete):285-296.
[10]刘晶晶,汪苹,王欢.一株异养硝化-好氧反硝化菌的脱氮性能研究[J].环境科学研究,21(3).
[11] Hwang J H,Oleszkiewicz J A. Effect of Cold-Temperature Shock on Nitrification[J]. Water Environment Research,2007,79(9):964-968.
[12] Park J J,Byun I G,Park S R,et al. Nitrifying bacterial communities and its activities in aerobic biofilm reactors under different temperature conditions[J]. Korean Journal of Chemical Engineering,2008,25(6):1448-1455.
[13] Kim J H,Guo X,Park H S. Comparison study of the effects of temperature and free ammonia concentration on nitrification and nitrite accumulation[J]. PROCESS BIOCHEMISTRY,2008,43(2):154-160.
[14]于大禹,张琳颖,高波.异养硝化-好氧反硝化菌异养硝化性能的影响因素[J].化工进展,2012,31(12):2797-2800.
[15] Taylor S M,Yiliang H E,Zhao B,et al. Heterotrophic ammonium removal characteristics of an aerobic heterotrophic nitrifying-denitrifying bacterium,Providencia rettgeri YL[J]. Journal of Environmental Sciences,2009,21(10):1336-1341.
[16] Kundu P,Pramanik A,Dasgupta A,et al. Simultaneous Heterotrophic Nitrification and Aerobic Denitrification by Chryseobacterium sp. R31 Isolated from Abattoir Wastewater[J]. BioMed Research International,2014,2014:1-12.
[17] Nitrogen removal characteristics of a heterotrophic nitrifier Acinetobacter junii YB and its potential application for the treatment of high-strength nitrogenous wastewater[J]. Bioresource Technology,2015,193:227-233.