山东地区10株H9N2亚型禽流感病毒HA和NA基因的遗传演化分析
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摘要
为了解2016年山东地区H9N2亚型禽流感病毒的变异情况,选取分离鉴定的10株H9N2亚型禽流感病毒分离毒株,分别对其HA和NA基因进行序列测定和分子特性分析,并应用Mega 5.0软件绘制相应的基因氨基酸进化树,进行遗传进化分析。结果表明:10株H9N2分离毒的HA裂解位点氨基酸均为RSSR/GLF,具有典型低致病性AIV HA基因的特征;其第226位氨基酸均为亮氨酸(L),因此具有结合人流感受体的分子特性。同时这10株病毒的NA基因颈部均有9个核苷酸的缺失,且NA的潜在糖基化位点均不超过8个。进化树分析表明,2016年山东地区H9N2流行毒株HA和NA基因均属A/Chicken/Beijing/1/1994亚群,并且同属于近几年在中国鸡群中流行的G57分支。
To evaluate the genetic variations of H9 N2 Avian influenza virus(AIV) circulating in 2016 in Shandong province, HA and NA genes of 10 isolates of H9 N2 AIV obtained in 2016 were sequenced for phylogenetic analysis using Mega 5.0 software. The results showed that the cleavage sites of their HA genes were all RSSR/GLF, showing the typical gene character of LPAIVs. The HA possessed leucine at 226 th amino acid position, indicating a human influenza receptor binding characteristic. The NA genes of these 10 isolates had a 9 bp deletion and their potential glycosylation sites were not more than 8. Phylogenetic analysis of HA and NA genes of these H9 N2 AIV isolates indicated that all of them belonged to the A/Chicken/Beijing/1/1994 subgroup and G57 lineage widely circulating among Chinese chicken populations in recent years.
To evaluate the genetic variations of H9 N2 Avian influenza virus(AIV) circulating in 2016 in Shandong province, HA and NA genes of 10 isolates of H9 N2 AIV obtained in 2016 were sequenced for phylogenetic analysis using Mega 5.0 software. The results showed that the cleavage sites of their HA genes were all RSSR/GLF, showing the typical gene character of LPAIVs. The HA possessed leucine at 226 th amino acid position, indicating a human influenza receptor binding characteristic. The NA genes of these 10 isolates had a 9 bp deletion and their potential glycosylation sites were not more than 8. Phylogenetic analysis of HA and NA genes of these H9 N2 AIV isolates indicated that all of them belonged to the A/Chicken/Beijing/1/1994 subgroup and G57 lineage widely circulating among Chinese chicken populations in recent years.
引文
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[12]Butt K M,Smith G J,Chen H,et al.Human infection with an avian H9N2 influenza A virus in Hong Kong in2003[J].J Clin Microbiol,2005,43(11):5760-5767.
[2]刘海霞,王艳晓,孟小宾,等.我国部分地区H9N2亚型禽流感病毒HA基因遗传进化分析[J].中国家禽,2014,36(21):57-59,62.
[3]申伟霞,田巧珍,陈圆,等.H3、H9亚型禽流感病毒双重荧光定量PCR方法的建立及初步应用[J].中国动物传染病学报,2014,22(1):16-24.
[4]王宝林,刘波,陈玲,等.流感病毒唾液酸受体在人、小鼠、鸡及鸭气管和肺组织分布的定性与定量分析[J].中国科学:生命科学,2012,42(5):383-389.
[5]Colman P M,Laver W G,Varghese J N,et al.Threedimensional structure of a complex of antibody with influenza virus neuraminidase[J].Nature,1987,326(6111):358-363.
[6]万春和,傅光华,程龙飞,等.A/Chicken/Zhejiang/HJ/2007(H9N2)禽流感病毒8基因测序及遗传进化分析[J].农业生物技术学报,2009,17(5):750-757.
[7]葛杰,葛菲菲,刘健,等.活禽批发市场内家禽停留时间与H9亚型禽流感病毒检出的关系[J].中国动物传染病学报,2017,25(1):32-35.
[8]索朗斯珠,邹威,王灿,等.禽流感病毒H9N2亚型西藏分离株HA和NA基因的克隆与序列分析[J].畜牧兽医学报,2010,41(12):1659-1666.
[9]刘金华,吴清民,陈福勇,等.鸡源株与人源株H9N2流感病毒血凝素受体结合位点氨基酸比较与分析[J].中国预防兽医学报,2003,25(6):416-418.
[10]Sun Y,Pu J,Jiang Z,et al.Genotypic evolution and antigenic drift of H9N2 influenza viruses in China from1994 to 2008[J].Vet Microbiol,2010,146(3-4):215-225.
[11]杨婧,刘宇卓,赵冬敏,等.2013年苏皖地区4株H9N2亚型禽流感病毒HA、NA基因的遗传演化分析[J].浙江农业学报,2015,27(11):1896-1902.
[12]Butt K M,Smith G J,Chen H,et al.Human infection with an avian H9N2 influenza A virus in Hong Kong in2003[J].J Clin Microbiol,2005,43(11):5760-5767.