处理条件对天然绿色棉色素抗氧化活性的影响
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摘要
基于天然绿色棉色素含有多酚类物质以及其结构的不稳定性,采用清除1,1-二苯基-2-苦基肼(DPPH)自由基法,探讨温度、光辐射、酸碱条件对绿色棉色素的抗氧化活性的影响。结果表明,高碱条件对绿色棉色素的抗氧化影响较大。经p H值为13的碱溶液处理后,色素的DPPH自由基清除率下降至2.97%,调回中性后,自由基清除能力恢复至原先的81.38%,说明碱性条件导致抗氧化性的消失大部分可以恢复。结合紫外光谱可以看出,碱性条件下色素主体结构不变,但部分组分结构发生了不可逆的破坏。光辐照对天然绿色棉色素的抗氧化活性有明显的影响,日晒5 d后,绿色棉色素自由基清除能力下降57.10%。高温150℃处理1 h对绿色棉色素的自由基清除能力影响不大。
Based on polyphenols pigment of natural green cotton and its structural instability,the1-diphenyl-2-picrylhydtazyl(DPPH) radical scavenging method was used to study the effects of temperature,light irradiation and acid-base condition on the antioxidant activity of green cotton pigment.The results showed that high alkali conditions had great influence on the antioxidant stability of natural green cotton.The DPPH radical scavenging activity of green cotton pigment decreased to 2.97% when the pigment was treated in the alkali solution with the p H value of 13.When adjusted to be neutral,green cotton pigment recovered to 81.38% of its original radical scavenging capability,indicating that most of radical scavenging capability lost resulted from the alkaline condition was recoverable.The ultraviolet spectra measurements demonstrated that the main structure of pigment was unchanged under the alkaline condition,but some components structure of the pigment had changed irreversibly.Light irradiation had significant effect on antioxidant activity of the pigment of naturally green cotton.The radical scavenging capability of green cotton pigment dropped to 57.10% when the pigment was exposed to the sun light for5 d.It was found that no significant change in radical scavenging capability of green cotton pigment was caused when treating the pigment at 150 ℃ for 1 h.
Based on polyphenols pigment of natural green cotton and its structural instability,the1-diphenyl-2-picrylhydtazyl(DPPH) radical scavenging method was used to study the effects of temperature,light irradiation and acid-base condition on the antioxidant activity of green cotton pigment.The results showed that high alkali conditions had great influence on the antioxidant stability of natural green cotton.The DPPH radical scavenging activity of green cotton pigment decreased to 2.97% when the pigment was treated in the alkali solution with the p H value of 13.When adjusted to be neutral,green cotton pigment recovered to 81.38% of its original radical scavenging capability,indicating that most of radical scavenging capability lost resulted from the alkaline condition was recoverable.The ultraviolet spectra measurements demonstrated that the main structure of pigment was unchanged under the alkaline condition,but some components structure of the pigment had changed irreversibly.Light irradiation had significant effect on antioxidant activity of the pigment of naturally green cotton.The radical scavenging capability of green cotton pigment dropped to 57.10% when the pigment was exposed to the sun light for5 d.It was found that no significant change in radical scavenging capability of green cotton pigment was caused when treating the pigment at 150 ℃ for 1 h.
引文
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[2]李海祥,唐志荣,李丹,等.天然绿色棉色素的提取与光谱性质研究[J].浙江理工大学报,2012,29(1):32-35.LI Haixiang,TANG Zhirong,LI Dan,et al.Extraction of pigment in naturally green cotton and spectral properties research[J].Journal of Zhejiang Institute of Science and Technology,2012,29(1):32-35.
[3]SEYOUM A,ASRES K,EI-FIKY FK.Structure-radicals cavenging activity relationships of flavonoids[J].Phytochemistry,2006,67(18):2058-2070.
[4]SCHMUTZ A A.Caffeoyl-fatty acid-glycerol ester from wax associated with green cotton fibre suberin[J].Phytochemistry,1994,36(6):1343-1346.
[5]彭长连,陈少薇,林植芳,等.用清除有机自由基DPPH法评价植物抗氧化能力[J].生物化学与生物物理进展,2000,27(6):658-661.PENG Changlian,CHEN Shaowei,LIN Zhifang,et al.Detection of antioxidative capacity in plants by scavenging organic free radical DPPH[J].Progress in Biochemistry and Biophysics,2000,27(6):658-661.
[6]顾艳,周文龙.干热处理温度对天然彩色棉色泽性能的影响[J].纺织学报,2009,30(1):60-63.GU Yan,ZHOU Wenlong.Effect of dry heat treatment on shade of naturally colored cotton[J].Journal of Textile Research,2009,30(1):60-63.
[7]张镁,胡伯陶.绿色彩棉色素稳定性研究[J].印染,2003,29(3):1-6.ZHANG Mei,HU Botao.A study on colorant stability of naturally colored cotton[J].China Dye&Finishing,2003,29(3):1-6.
[8]罗少宏.天然棕色棉色素组分、结构及性能研究[D].杭州:浙江理工大学,2014:47-49.LUO Shaohong.Identification of pigment from naturally brown cotton and performance study of brown cotton fiber[D].Hangzhou:Zhejiang Sci-Tech University,2014:47-49.
[9]韦献雅,殷丽琴,钟成,等.DPPH法评价抗氧化活性研究进展[J].食品科学,2014,35(9):317-322.WEI Xianya,YIN Liqin,ZHONG Cheng,et al.Advances in the DPPH radical scavenging assay for antioxidant activity evaluation[J].Food Science,2014,35(9):317-322.
[10]GLIN ILHAMI.Antioxidant activity of caffeic acid(3,4-dihydroxycinnamic acid)[J].Toxicology,2006,217(2):213-220.
[11]VILLANO D,FEMNDEZ-PACHN M S,MOYM,et al.Radical scavenging ability of polyphenolic compounds towards DPPH free radical[J].Talanta,2007,71(1):233-235.
[12]梁光雁,顾肇文.天然彩色棉色素变化机理探讨及其应用研究[J].纺织学报,2004,25(3):11-13.LIANG Guangyan,GU Zhaowen.Inquiry and apply the mechanism of coloring matter transformation in natural colored cotton[J].Journal of Textile Research,2004,25(3):11-13.
[13]韩光亭,郑春晓,王秋美,等.p H值对彩棉纤维颜色的影响[J].青岛大学学报(工程技术版),2003,18(1):55-57.HAN Guangting,ZHENG Chunxiao,WANG Qiumei,et al.The influence of p H on the color of colored cotton[J].Journal of Qingdao University(Engineering and Technology Edition),2003,18(1):55-57.
[14]冯鸿杰.天然彩色棉纤维色素物质的鉴定及其代谢机制研究[D].石河子:石河子大学,2014:55-60.FENG Hongjie.Study on structure profile and metabolic mechanism of pigment in colored cotton fiber[D].Shihezi:Shihezi University,2014:55-60.
[15]凌文华,郭红辉.植物花色苷[M].北京:科学出版社,2009:56-58.LING Wenhua,GUO Honghui.Plant Anthocyanins[M].Beijing:Science Press,2009:56-58.
[16]刘湘,汪秋安.天然产物化学[M].北京:化学工业出版社,2005:65-77.LIU Xiang,WANG Qiuan.Natural Products Chemistry[M].Beijing:Chemistry Industry Press,2005: