HAND2在子宫内膜癌中的表达及意义
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摘要
目的研究子宫内膜癌组织中心脏神经脊衍生物表达转录因子2(heart and neural crest derivatives-expressed transcript2,HAND2)的表达及意义。方法采用免疫组化方法检测86例子宫内膜样腺癌、30例子宫内膜不典型增生及28例正常子宫内膜组织中HAND2蛋白的表达情况并分析其与临床病理的关系。结果 HAND2蛋白在28例正常子宫内膜组织、30例子宫内膜不典型增生组织及86例子宫内膜样腺癌组织中的表达呈下降趋势(89.3%,60.0%,34.9%),HAND2蛋白在正常子宫内膜组织、子宫内膜不典型增生组织及子宫内膜样腺癌组织间的表达差异有统计学意义(P<0.05);HAND2蛋白在子宫内膜样腺癌组织中的表达与病理分期和肌层浸润有关(P<0.05),与组织学分级及淋巴结转移情况无关(P>0.05)。结论HAND2蛋白在子宫内膜样腺癌中表达下调可能与子宫内膜癌的发生发展有关。
Objective To investigate the expression and implication of HAND2 in endometrial carcinoma.Methods Expression of HAND2 was detected with immunohistochemistry SP assay in 28 cases of endometrial tissue,30 cases of atypical endometrial hyperplasia tissue and 86 cases of endometrial cancer,and its relationships with clinicopathological features were analyzed.Results The positive expression rate of HAND2 protein in endometrial cancer tissue was lower than that in endometrial tissue and atypical endometrial hyperplasia tissue(34.9% vs 89.3%,60.0%,P < 0.05).The expression of HAND2 protein in endometrial cancer was related with pathological stage and myometrial invasion(P < 0.05),but not with lymph node metastasis and pathological grading(P > 0.05).Conclusion The downregulation of HAND2 protein expression in endometrial carcinoma may be related to the development of endometrial carcinoma.
Objective To investigate the expression and implication of HAND2 in endometrial carcinoma.Methods Expression of HAND2 was detected with immunohistochemistry SP assay in 28 cases of endometrial tissue,30 cases of atypical endometrial hyperplasia tissue and 86 cases of endometrial cancer,and its relationships with clinicopathological features were analyzed.Results The positive expression rate of HAND2 protein in endometrial cancer tissue was lower than that in endometrial tissue and atypical endometrial hyperplasia tissue(34.9% vs 89.3%,60.0%,P < 0.05).The expression of HAND2 protein in endometrial cancer was related with pathological stage and myometrial invasion(P < 0.05),but not with lymph node metastasis and pathological grading(P > 0.05).Conclusion The downregulation of HAND2 protein expression in endometrial carcinoma may be related to the development of endometrial carcinoma.
引文
[1]Stubert J,Gerber B.Current issues in the diagnosis and treatment of endometrial carcinoma[J].Geburtshilfe Frauenheilkunde,2016,76(02):170-175.
[2]Murali R,Soslow RA,Weigelt B.Classification of endometrial carcinoma:more than two types[J].Lancet Oncol,2014,15(7):e268-e278.
[3]Ellenson LH,Ronnett BM,Soslow RA,et al.Endometrial carcinoma[J].New Engl J,2011,335(2):214-215.
[4]Mc Fadden DG,Barbosa AC,Richardson JA,et al.The HAND1and HAND2 transcription factors regulate expansion of the embryonic cardiac ventricles in a gene dosage-dependent manner[J].Development,2005,132(1):189-201.
[5]Franasiak J,Hubbs J,Olorvida M,et al.HAND2 expression is increased in endometrial hyperplasia/cancer[J].Gynecol Oncol,2013,130(1):e89-e89.
[6]Yin XH,Shi M,Wu H,et al.Role of HAND2 gene and protein expression in endometrial carcinoma[J].Eur J Gynaecol Oncol,2017,38(1):95-96.
[7]Firulli BA,Krawchuk D,Centonze VE,et al.Altered Twist1and Hand2 dimerization is associated with Saethre-Chotzen syndrome and limb abnormalities[J].Nature Genet,2005,37(4):373-381.
[8]Lucas ME,Muller FR,Henion PD,et al.The b HLH transcription factor hand2 is essential for noradrenergic differentiation of sympathetic neurons[J].Development,2006,133(20):4015-4016.
[9]Mc Fadden DG,Barbosa AC,Richardson JA,et al.The HAND1and HAND2 transcription factors regulate expansion of the embryonic cardiac ventricles in a gene dosage-dependent manner[J].Development,2005,132(1):189-201.
[10]Morikawa Y,D’Autréaux F,Gershon MD,et al.HAND2 determines the noradrenergic phenotype in the mouse sympathetic nervous system[J].Dev Biol,2007,307(1):114-126.
[11]Hendershot TJ,Liu H,Sarkar AA,et al.Expression of HAND2is sufficient for neurogenesis and cell type-specific gene expression in the enteric nervous system[J].Dev Dyn,2010,236(1):93-105.
[12]陈美霞,孟元光,韩为东,等.DNA甲基化在子宫内膜癌中的研究进展[J].国外医学(妇产科学分册),2007,34(6):421-424.
[13]石敏,尹香花.HAND2甲基化在子宫内膜癌中的研究现状[J].医学综述,2015,21(15):2744-2746.
[2]Murali R,Soslow RA,Weigelt B.Classification of endometrial carcinoma:more than two types[J].Lancet Oncol,2014,15(7):e268-e278.
[3]Ellenson LH,Ronnett BM,Soslow RA,et al.Endometrial carcinoma[J].New Engl J,2011,335(2):214-215.
[4]Mc Fadden DG,Barbosa AC,Richardson JA,et al.The HAND1and HAND2 transcription factors regulate expansion of the embryonic cardiac ventricles in a gene dosage-dependent manner[J].Development,2005,132(1):189-201.
[5]Franasiak J,Hubbs J,Olorvida M,et al.HAND2 expression is increased in endometrial hyperplasia/cancer[J].Gynecol Oncol,2013,130(1):e89-e89.
[6]Yin XH,Shi M,Wu H,et al.Role of HAND2 gene and protein expression in endometrial carcinoma[J].Eur J Gynaecol Oncol,2017,38(1):95-96.
[7]Firulli BA,Krawchuk D,Centonze VE,et al.Altered Twist1and Hand2 dimerization is associated with Saethre-Chotzen syndrome and limb abnormalities[J].Nature Genet,2005,37(4):373-381.
[8]Lucas ME,Muller FR,Henion PD,et al.The b HLH transcription factor hand2 is essential for noradrenergic differentiation of sympathetic neurons[J].Development,2006,133(20):4015-4016.
[9]Mc Fadden DG,Barbosa AC,Richardson JA,et al.The HAND1and HAND2 transcription factors regulate expansion of the embryonic cardiac ventricles in a gene dosage-dependent manner[J].Development,2005,132(1):189-201.
[10]Morikawa Y,D’Autréaux F,Gershon MD,et al.HAND2 determines the noradrenergic phenotype in the mouse sympathetic nervous system[J].Dev Biol,2007,307(1):114-126.
[11]Hendershot TJ,Liu H,Sarkar AA,et al.Expression of HAND2is sufficient for neurogenesis and cell type-specific gene expression in the enteric nervous system[J].Dev Dyn,2010,236(1):93-105.
[12]陈美霞,孟元光,韩为东,等.DNA甲基化在子宫内膜癌中的研究进展[J].国外医学(妇产科学分册),2007,34(6):421-424.
[13]石敏,尹香花.HAND2甲基化在子宫内膜癌中的研究现状[J].医学综述,2015,21(15):2744-2746.