Optimization of Extraction Technology and Determination of Content of Total Phenols of Leaves in Acanthopanax giraldii Harms
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摘要
[Objectives] To determine the optimum extraction technology for total phenols of leaves in Acanthopanax giraldii Harms.[Methods]The single factor test and orthogonal design were adopted to study the extraction technology for total phenols of leaves in Acanthopanax giraldii Harms. [Results]The optimum extraction conditions were determined as follows: extraction solvent( 70% methanol),solid-liquid ratio( 1∶ 35),and reflux extraction time( 60 min). Under these conditions,the yield of crude extract from total phenols of leaves in Acanthopanax giraldii Harms was 38. 67 mg/g.[Conclusions] The technology was simple,and of low cost and high extraction rate,suitable for the extraction of total phenols of leaves in Acanthopanax giraldii Harms.
[Objectives] To determine the optimum extraction technology for total phenols of leaves in Acanthopanax giraldii Harms.[Methods]The single factor test and orthogonal design were adopted to study the extraction technology for total phenols of leaves in Acanthopanax giraldii Harms. [Results]The optimum extraction conditions were determined as follows: extraction solvent( 70% methanol),solid-liquid ratio( 1∶ 35),and reflux extraction time( 60 min). Under these conditions,the yield of crude extract from total phenols of leaves in Acanthopanax giraldii Harms was 38. 67 mg/g.[Conclusions] The technology was simple,and of low cost and high extraction rate,suitable for the extraction of total phenols of leaves in Acanthopanax giraldii Harms.
[Objectives] To determine the optimum extraction technology for total phenols of leaves in Acanthopanax giraldii Harms.[Methods]The single factor test and orthogonal design were adopted to study the extraction technology for total phenols of leaves in Acanthopanax giraldii Harms. [Results]The optimum extraction conditions were determined as follows: extraction solvent( 70% methanol),solid-liquid ratio( 1∶ 35),and reflux extraction time( 60 min). Under these conditions,the yield of crude extract from total phenols of leaves in Acanthopanax giraldii Harms was 38. 67 mg/g.[Conclusions] The technology was simple,and of low cost and high extraction rate,suitable for the extraction of total phenols of leaves in Acanthopanax giraldii Harms.
引文
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[10]ZHAI X,CHEN YJ,ZHAO W,et al.Extraction and determination of total saponins in tender leaves of Acanthopanax giraldii Harms.medicinal plant[J].2016,7(7-8):9-12.
[11]YANG L,NUERBIYE,LI H.Determination of total polyphenols in different parts of jujubae fruetus growing in Xinjiang[J].Food Research and Development,2016,37(1):152-156.(in Chinese).
[2]LI SZ.Compendium of materia medica[M].Beijing:People's Medical Publishing House,1982:2110.(in Chinese).
[3]XIE ZW,YU WC.Name of Chinese herbal medicines[M].Beijing:People's Medical Publishing House,1996:611.(in Chinese).
[4]CHEN YJ,ZHAO W,ZHAI X,et al.Determination of chlorogenic acid from Acanthopanacis giraldii tender leaves by UPLC[J].West China Journal of Pharmaceutical Sciences,2016,31(2):199-201.(in Chinese).
[5]ZHONG P,WANG PP.Optimization of microwave extraction of total phenols from magnolia bark by orthogonal array design[J].Food Science,2013,34(8):82-84.(in Chinese).
[6]YAN XJ,GU ZX,LU FL,et al.Determination of total polyphenol from Callicarpa nudiflora by Folin-phonel colorimetry[J].China Journal of Experimental Traditional Medical Formulae,2013,19(18):74-78.(in Chinese).
[7]SHANG HM,GUO W,PAN D,et al.Effects of drying methods on polyphenol contents and antioxidant activities of chicory root[J].Food Science,2015,36(1):84-88.(in Chinese).
[8]ZHAO XY,LI B,YANG ZM,et al.Extraction and determination of total phenolic acids in Leontopodium franchetii at different harvest time[J].China Journal of Experimental Traditional Medical Formulae,2015,21(9):19-21.(in Chinese).
[9]HU JP,YANG JH,WANG XL,et al.Determination of total polyphenols in different parts of Elaeagnus angustifolia fruit growing in Xinjiang[J].Food Science,2010,31(6):220-222.(in Chinese).
[10]ZHAI X,CHEN YJ,ZHAO W,et al.Extraction and determination of total saponins in tender leaves of Acanthopanax giraldii Harms.medicinal plant[J].2016,7(7-8):9-12.
[11]YANG L,NUERBIYE,LI H.Determination of total polyphenols in different parts of jujubae fruetus growing in Xinjiang[J].Food Research and Development,2016,37(1):152-156.(in Chinese).