短柄五加显微及薄层鉴定研究
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摘要
目的:对短柄五加显微及薄层鉴定方法进行研究。方法:采用光学显微镜观察其组织显微特征;薄层色谱方法对其中指标成分进行鉴定。结果:短柄五加在水中反复煮,煮至沉水,软化效果较好;水粘片烤36 h时,脱片率低;染色时间为7 h染色效果较好;薄层鉴别采用硅胶GF254薄层板,以三氯甲烷-甲醇-水(10∶6∶2)10℃静置的下层溶液为展开剂展开,置紫外光灯(254 nm)下检视,有很好重现性。结论:该方法可以作为短柄五加显微及薄层鉴定方法应用于实验研究,为进一步开发利用短柄五加提供参考。
objective:The microscopic methods and TLC for Acanthopanax brachypus Harms were studied. Methods:The microscopic characteristics of the tissue were observed by optical microscope,identification of indicator components by TLC.Resulst:The Acanthopanax brachypus Harms has better softening effect after repeated boiling water,when the water sticky slices are roasted 36 h,defragment rate was low,the dyeing time was better by 7 h staining. Thin layer of silica gel GF254 thin plate,the lower level solution of trichloromethane methanol water(10 ∶6 ∶2) at 10 degrees centigrade was expanded. The ultraviolet lamp(254 nm) was inspected under the UV lamp,which showed good reproducibility. Conclusion:This method could be used as a microscopic methodand TLC for Acanthopanax brachypus Harms,which provided a basis for further development and utilization of Acanthopanax brachypus Harms.
objective:The microscopic methods and TLC for Acanthopanax brachypus Harms were studied. Methods:The microscopic characteristics of the tissue were observed by optical microscope,identification of indicator components by TLC.Resulst:The Acanthopanax brachypus Harms has better softening effect after repeated boiling water,when the water sticky slices are roasted 36 h,defragment rate was low,the dyeing time was better by 7 h staining. Thin layer of silica gel GF254 thin plate,the lower level solution of trichloromethane methanol water(10 ∶6 ∶2) at 10 degrees centigrade was expanded. The ultraviolet lamp(254 nm) was inspected under the UV lamp,which showed good reproducibility. Conclusion:This method could be used as a microscopic methodand TLC for Acanthopanax brachypus Harms,which provided a basis for further development and utilization of Acanthopanax brachypus Harms.
引文
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[12]谭五丰,袁艳秋,于笛笛,等.刺五加与短梗五加中紫丁香苷、刺五加苷E及异嗪皮啶含量比较[J].现代食品科技,2017(10):302-307.
[2]刘庆玲,刘建华,刘金杰.中草药资源开发利用的研究现状与进展[J].生物学通报,2001,36(5):45-46.
[3]丁永辉.甘肃省中药材标准[M].兰州:甘肃文化出版社,2009:59-61.
[4]胡浩斌,樊君.短柄五加化学成分的结构鉴定及其抑菌活性[J].中国医院药学杂志,2008,28(16):1344-1347.
[5]李建银,杨永建.短柄五加的现代研究进展[C]//全国第9届天然药物资源学术研讨会论文集.广州:中国自然资源学会天然药物资源专业委员会,2010:716-719.
[6]国家药典委员会.中华人民共和国药典·2015年版一部[S].北京:中国医药科技出版社,2015:57-59.
[7]田晨霞,张咏梅,马晖玲.草地早熟禾胚胎结构石蜡切片制作方法初探[J].草业科学,2013,30(12):1980-1986.
[8]尚坤,王承南,陈昊,等.不同粘片剂对油茶叶片石蜡切片效果的影响[J].安徽大学学报(自科版),2016,40(5):105-108.
[9]任成林,田勇,梁淑珍.动物组织H.E染色石蜡切片技术的改进[J].河北北方学院学报(自然科学版),2007,23(1):41-45.
[10]王金平,张俊梅.白头翁花的石蜡切片制作[J].信阳师范学院学报·自然科学版,2008,21(4):573-576.
[11]潘静岚.槲寄生药材中紫丁香苷薄层鉴别方法研究[J].西部中医药,2015(4):30-32.
[12]谭五丰,袁艳秋,于笛笛,等.刺五加与短梗五加中紫丁香苷、刺五加苷E及异嗪皮啶含量比较[J].现代食品科技,2017(10):302-307.