摘要
目的建立测定人工牛黄甲硝唑胶囊中胆红素含量高效液相色谱(HPLC)法。方法色谱柱采用Agilent ZORBAX SB-C18柱(150 mm×4. 6 mm,5μm),流动相为乙腈-1%冰醋酸溶液(95∶5),检测波长为453 nm,柱温为30℃,流速为1. 0 mL/min,进样量为5μL。结果胆红素质量浓度在0. 442~11. 05μg/m L(r=0. 999 9)范围内与峰面积线性关系良好;平均回收率为99. 45%,RSD为1. 02%(n=9)。结论该法简便、精密度好、结果准确,可用于人工牛黄甲硝唑胶囊中胆红素的含量测定。
Objective To establish an HPLC method for the content determination of bilirubin in Galculus Bovis and Metronidazole Capsules. Methods The chromatographic column was Agilent ZORBAX SB-C18 column( 150 mm × 4. 6 mm,5 μm),the mobile phase was acetonitrile-1 % acetic acid( 95 ∶ 5),the detection wavelength was 453 nm,the column temperature was 30 ℃,the flow rate was1. 0 mL/min and the sample size was 5 μL. Results The bilirubin showed good linear relationship with peak area in the range of0. 442-11. 05 μg/m L( r = 0. 999 9),the average recovery was 99. 45% and RSD was 1. 02%( n = 9). Conclusion The method is simple,accurate with good reproducibility,which can be used for the content determination of bilirubin in Galculus Bovis and Metronidazole Capsules.
引文
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