盐穗木种子和幼苗对NaHCO_3胁迫的响应
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摘要
以盐穗木种子和幼苗为材料,研究NaHCO_3胁迫下盐穗木种子的萌发特性和幼苗的生理变化,以确定其对NaHCO_3胁迫的适应能力。结果表明:1)低浓度(100mmol/L)的NaHCO_3对盐穗木种子萌发有刺激作用,促进其在较短时间内达到最终萌发率(与ck无显著差异);中高浓度(200~600mmol/L)的NaHCO_3显著抑制盐穗木种子萌发,且随着NaHCO_3浓度的增加,盐穗木种子最终萌发率显著下降,平均萌发时间延长,盐穗木种子的耐NaHCO_3阈值和极限值分别为318mmol/L和634mmol/L;2)与对照(0mmol/L)相比,在NaHCO_3浓度为200~400mmol/L范围内,盐穗木幼苗的相对电导率增加,但丙二醛(MDA)含量无显著变化,脯氨酸含量、可溶性糖含量、过氧化氢酶(CAT)活性、过氧化物酶(POD)活性呈增加的趋势,而NaHCO_3为600mmol/L时,MDA含量显著增加;3)综合NaHCO_3环境下盐穗木种子萌发特性和幼苗生理指标变化,盐穗木可在NaHCO_3浓度略低于300mmol/L的环境下较好地发芽、生长,高于该浓度则会受到抑制。
The experiments were conducted with seed and seedling of Halostachys caspica as materials under different NaHCO_3 treatments to evaluate its adaptability by examining germination characteristics of seed and physiological response of seedling.The results show that:1)Low concentration of NaHCO_3(100 mmol/L)had a stimulating effect on Halostachys caspica seed germination,that promoted seed reached final germination rate in a relatively short time(no significant difference with ck),while medium and high concentration of NaHCO_3(200-600 mmol/L)inhibited significantly seed germination.Final germination rate decreased significantly and mean germination time prolonged in Halostachys caspica with concentration of NaHCO_3 increasing.The NaHCO_3 threshold and limit value of Halostachys caspica seed were 318 mmol/L and 634 mmol/L respectively.2)In the range of200-400 mmol/L NaHCO_3,compared with control(0),the relative conductivity of the Halostachys caspicaseedlings was increased but there was no significant change in the content of MDA,and proline content,soluble sugar content,soluble protein content,CAT activity,and POD activity showed an increasing trend in Halostachys caspica seedlings.MDA content increased significantly while concentration of NaHCO_3 was 600 mmol/L.3)Comprehensively consulted with characteristics of seed germination and physiological indexes changes of Halostachys caspicaseedlings under NaHCO_3 treatments,Halostachys caspica germinated and growth well available at concentration of NaHCO_3 about below 300 mmol/L,while it will be inhibited when concentration of NaHCO_3 was higher than 300 mmol/L.
The experiments were conducted with seed and seedling of Halostachys caspica as materials under different NaHCO_3 treatments to evaluate its adaptability by examining germination characteristics of seed and physiological response of seedling.The results show that:1)Low concentration of NaHCO_3(100 mmol/L)had a stimulating effect on Halostachys caspica seed germination,that promoted seed reached final germination rate in a relatively short time(no significant difference with ck),while medium and high concentration of NaHCO_3(200-600 mmol/L)inhibited significantly seed germination.Final germination rate decreased significantly and mean germination time prolonged in Halostachys caspica with concentration of NaHCO_3 increasing.The NaHCO_3 threshold and limit value of Halostachys caspica seed were 318 mmol/L and 634 mmol/L respectively.2)In the range of200-400 mmol/L NaHCO_3,compared with control(0),the relative conductivity of the Halostachys caspicaseedlings was increased but there was no significant change in the content of MDA,and proline content,soluble sugar content,soluble protein content,CAT activity,and POD activity showed an increasing trend in Halostachys caspica seedlings.MDA content increased significantly while concentration of NaHCO_3 was 600 mmol/L.3)Comprehensively consulted with characteristics of seed germination and physiological indexes changes of Halostachys caspicaseedlings under NaHCO_3 treatments,Halostachys caspica germinated and growth well available at concentration of NaHCO_3 about below 300 mmol/L,while it will be inhibited when concentration of NaHCO_3 was higher than 300 mmol/L.
引文
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[12]韩占江,程龙,李志军,等.塔里木盆地7种藜科植物种子萌发特性的研究[J].种子,2016,35(9):1-5.
[13]刘会良,宋明方,段士民,等.古尔班通古特沙漠南缘32种藜科植物种子萌发策略初探[J].中国沙漠,2012,32(2):413-420.
[14]刘一明,程凤枝,王齐,等.四种暖季型草坪植物的盐胁迫反应及其耐盐阈值[J].草业学报,2009,18(3):192-199.
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[2]Zhao K,Scmg J,Feng U,et al.Species,types,distribution,and economic potential of halophytes in China[J].Plant Soi1,2011,342(1-2):495-509.
[3]张冀,杜驰,张富春.盐胁迫下盐穗木DNA聚合酶λ基因的克隆和表达分析[J].新疆农业科学,2017,54(2):361-370.
[4]王虹,齐政,张富春.不同浓度盐胁迫下盐穗木叶片结构的比较观察[J].新疆农业科学,2016,53(11):2 098-2 105.
[5]王梅,赵晨光,王莹,等.盐穗木种群空间点格局对地下水埋深的响应[J].林业科学,2015,51(11):17-24.
[6]王丽敏,张霞,张富春.植物激素赤霉素和萘乙酸对盐穗木种子萌发的影响[J].新疆农业科学,2014,51(3):504-510.
[7]韩张雄,李利,徐新文,等.盐穗木对盐渍荒漠区不同土壤水盐含量的适应机制研究[J].西北农林科技大学学报(自然科学版),2014,42(2):167-172,178.
[8]赵金香,贾琦珍,张国峰,等.盐穗木对卡拉库尔羊临床指标及血液生化指标的影响[J].江苏农业科学,2011,39(3):277-280.
[9]阿斯古丽·伊斯马伊力,牙库甫江·阿西木,张霞,等.盐穗木的染色体数目与核型分析[J].草业科学,2013,30(9):1 366-1 368.
[10]王丽敏,张霞,张富春.水盐胁迫对盐穗木种子萌发的影响[J].种子,2013,32(12):6-10.
[11]赵恩峰,李玲,曾幼玲.盐胁迫对盐生植物盐穗木生理特性的变化分析[J].新疆农业科学,2012,49(4):716-722.
[12]韩占江,程龙,李志军,等.塔里木盆地7种藜科植物种子萌发特性的研究[J].种子,2016,35(9):1-5.
[13]刘会良,宋明方,段士民,等.古尔班通古特沙漠南缘32种藜科植物种子萌发策略初探[J].中国沙漠,2012,32(2):413-420.
[14]刘一明,程凤枝,王齐,等.四种暖季型草坪植物的盐胁迫反应及其耐盐阈值[J].草业学报,2009,18(3):192-199.
[15]王学奎,黄见良主编.植物生理生化实验技术(第3版)[M].北京:高等教育出版社,2015.
[16]李合生主编.现代植物生理学(第3版)[M].北京:高等教育出版社,2012.