单独感染HCV与HCV/HIV共感染患者外周血T淋巴细胞分化分析
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摘要
目的探讨单独感染HCV与HCV/HIV共感染患者外周血T淋巴细胞分化情况。方法采集单独感染HCV患者(12例)、HCV/HIV共感染患者(27例)以及正常人群(13例)的外周静脉血,检测ALT、AST等生化指标;提取外周血单个核细胞(PBMC),通过流式细胞检测技术检测CD4+T和CD8+T细胞比例。结果 HCV/HIV共感染患者ALT水平[(53.08±9.78)U/L]显著高于单独感染HCV患者[(26.22±4.25)U/L],差异有统计学意义(P=0.0064)。处于中期分化状态的CD4+T细胞,在正常人群中所占比例为(35.13±3.24)%,显著高于单独感染HCV患者[(9.01±3.70)%]和HCV/HIV共感染患者[(8.10±2.14)%],差异有统计学意义(P<0.001)。正常人群、单独感染HCV患者与HCV/HIV共感染患者晚期分化的CD4+T细胞所占比例分别为(2.52±2.54)%、(14.30±9.85)%及(15.23±11.58)%,正常人群晚期分化的CD4+T细胞比例显著高于单感染HCV与HCV/HIV共感染患者的比例,差异有统计学意义(P<0.05),单独感染HCV与HCV/HIV共感染患者之间无差异。正常人群处于早期分化和中期分化的CD8+T细胞的比例分别为(42.73±2.08)%、(36.17±2.85)%,均显著高于单独感染HCV患者同期水平[(28.27±4.36)%、(13.15±4.23)%]及HCV/HIV共感染患者[(18.02±2.01)%、(16.60±3.06)%],差异均有统计学意义(P<0.05);单独感染HCV患者的早期分化CD8+T细胞比例也显著高于HCV/HIV共感染患者,差异有统计学意义(P<0.05)。而处于晚期分化的CD8+T细胞,正常人群所占比例为(12.02±1.54)%,显著低于单独感染HCV患者[(49.30±3.85)%]及HCV/HIV共感染患者[(54.23±2.58)%],差异有统计学意义(P<0.05)。结论单独感染HCV及HCV/HIV共感染影响T淋巴细胞的分化状态;HCV/HIV共感染影响处于早期分化的CD8+T细胞比例。
Objective To explore the effect of mono-infected HCV and co-infected HCV/HIV patients on the differentiation of peripheral blood T lymphocytes. Methods Peripheral blood of HCV patients(12 cases), HCV/HIV patients(27 cases)and healthy people(13 cases)were tested for the biochemical marker by chemistry analyzer, and the CD4+T cells and CD8+T cells were analyzed by FACS in PBMC. Results ALT level of co- infected HCV/HIV patients was(53.08±9.78)U/L, which was significantly higher than in the HCV group[(26.22±4.25)U/L, P = 0.0064]. The percentage of intermediate CD4+T cells was(9.01±3.70)% in the HCV group,(8.10±2.14)% in the HCV/HIV group, which was significantly lower than in the healthy group[(35.13 ± 3.24)%, P < 0.001]. The percentage of late CD4+T cells was(2.52 ±2.54)%,(14.30±9.85)%,(15.23±11.58)% respectively in the healthy group, HCV group and HCV/HIV group. The percentage of CD4+T cells in the healthy group was significantly lower than in the other two groups(P < 0.05), but there was no significant difference between HCV and HCV/HIV group. The percentage of early- stage CD8+T cell in the healthy group was(42.73±2.08)%, which was significantly higher than in the HCV group [(28.27±4.36)%] and HCV/HIV group[(18.02±2.01)%], P all<0.05. The percentage of early CD8+T cells in HCV group was higher than in the HCV/HIV group(P < 0.05). The percentage of intermediate CD8+T cell was(36.17 ± 2.85)%, which was significantly higher than in the HCV group [(13.15±4.23)%] and HCV/HIV group [(16.60±3.06)%], P < 0.05. While the percentage of late CD8+T cell in the healthy group was(12.02±1.54)%, which was significantly lower than in the HCV group [(49.30±3.85)%] and HCV/HIV group[(54.23±2.58)%], P < 0.05. Conclusion Mono-infected HCV and(or) HCV/HIV co-infection do affect on the differentiation of T lymphocytes. HCV/HIV co-infection can affect on the early CD8+T cells.
Objective To explore the effect of mono-infected HCV and co-infected HCV/HIV patients on the differentiation of peripheral blood T lymphocytes. Methods Peripheral blood of HCV patients(12 cases), HCV/HIV patients(27 cases)and healthy people(13 cases)were tested for the biochemical marker by chemistry analyzer, and the CD4+T cells and CD8+T cells were analyzed by FACS in PBMC. Results ALT level of co- infected HCV/HIV patients was(53.08±9.78)U/L, which was significantly higher than in the HCV group[(26.22±4.25)U/L, P = 0.0064]. The percentage of intermediate CD4+T cells was(9.01±3.70)% in the HCV group,(8.10±2.14)% in the HCV/HIV group, which was significantly lower than in the healthy group[(35.13 ± 3.24)%, P < 0.001]. The percentage of late CD4+T cells was(2.52 ±2.54)%,(14.30±9.85)%,(15.23±11.58)% respectively in the healthy group, HCV group and HCV/HIV group. The percentage of CD4+T cells in the healthy group was significantly lower than in the other two groups(P < 0.05), but there was no significant difference between HCV and HCV/HIV group. The percentage of early- stage CD8+T cell in the healthy group was(42.73±2.08)%, which was significantly higher than in the HCV group [(28.27±4.36)%] and HCV/HIV group[(18.02±2.01)%], P all<0.05. The percentage of early CD8+T cells in HCV group was higher than in the HCV/HIV group(P < 0.05). The percentage of intermediate CD8+T cell was(36.17 ± 2.85)%, which was significantly higher than in the HCV group [(13.15±4.23)%] and HCV/HIV group [(16.60±3.06)%], P < 0.05. While the percentage of late CD8+T cell in the healthy group was(12.02±1.54)%, which was significantly lower than in the HCV group [(49.30±3.85)%] and HCV/HIV group[(54.23±2.58)%], P < 0.05. Conclusion Mono-infected HCV and(or) HCV/HIV co-infection do affect on the differentiation of T lymphocytes. HCV/HIV co-infection can affect on the early CD8+T cells.
引文
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[2]刘金花,赵艳,孙焕琴,等.HCV对HIV/HCV共感染患者病情进展的影响[J].临床肝胆病杂志,2014,30:501-504.
[3]刘春云,孔蕾,普冬,等.HIV感染对HCV肝损害病毒水平和基因分型的影响[J].吉林医学,2010,31:2366-2367.
[4]Tovo CV,Becker SC,Almeida PR,et al.Progression of liver fibrosis in monoinfected patients by hepatitis C virus and coinfected by HCV and human immunodeficiency virus[J].Arq Gastroenterol,2013,50:19-22.
[5]Yi L,Zhao J,Lu J,et al.Gene expression profiling of CD4+T cells in treatment-naive HIV,HCV mono-or co-infected Chinese[J].Virol J,2014,13:11-27.
[6]刘俊,田波,李重熙,等.昆明地区HIV/HCV共感染者不同CD4水平肝功能对比研究[J].昆明医学院学报,2012,2:138-139.
[7]Coyle AJ,Gutierrez-Ramos JC.The expanding B7 superfamily:increasing complexity in e06timulatory signals regulating T cell function.Nat Immumol,2001,2:203-209.
[8]Appay V.The physiological role of cytotoxic CD4+T cells:the holy grail?[J].Clin Exp Immunol,2004,138:10-13.
[9]曹志红,蒋静,王心静,等.CD27和CD28在肺结核病人抗原特异性CD4+T细胞中的表达分析[J].中国医师杂志,2010,12:440-443.
[10]张永宏,赵艳,马丽娜,等.细胞毒性T细胞的功能与HIV/HCV重叠感染病情进展的关系[J].中华医学会第四次全国艾滋病、病毒性丙型肝炎暨全国热带病学术会议论文汇编,西安,2009,118.