AhHDA1异源表达对拟南芥根系生长的影响
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摘要
AhHDA1是花生中的组蛋白去乙酰化酶(histone deacetylase, HDAC),与拟南芥中的HDA6高度同源,二者都具有保守的组蛋白去乙酰化酶活性结构域。本文对7日龄野生型拟南芥(Col-0)、hda6突变体和AhHDA1异源表达株系AhHDA1/hda6的主根长度和侧根数量进行测量统计。结果表明,以hda6为对照, AhHDA1/hda6的主根生长速度明显减缓,侧根数量也显著减少; AhHDA1/hda6的分生区长度和伸长区的细胞长度与Col-0相近,与hda6突变体相比显著缩短;AhHDA1/hda6根部的细胞周期和生长素极性运输相关基因的表达水平皆显著低于hda6突变体。说明AhHDA1可能影响了生长素极性运输从而下调细胞周期相关基因的表达,抑制根尖分生区的细胞分裂和伸长区的细胞伸长,进而抑制拟南芥主根生长和侧根发生。
AhHDA1 is a histone deacetylase of peanut, which is highly homologous to HDA6 in Arabidopsis thaliana. Both AhHDA1 and AtHDA6 have conserved histone deacetylase activity domain. 7-day-old Col-0, hda6 mutant and AhHDA1/hda6 lines were obtained to analyze the length of primiry root and the number of lateral roots. The results showed that, the growth of primiry root of AhHDA1/hda6 was obviously slowed down and its number of lateral roots also decreased significantly compared to hda6 mutant. The root tip of three lines were observed by Leica DM6 fluorescence microscope, the results revealed that the length of meristem and elongation cells of AhHDA1/hda6 were significantly shortened compared with hda6 mutant, however, it had no significant difference compared with Col-0. Further detection of gene expression levels showed that, the expression of cell cycle-related and auxin polar transport-related genes in root of AhHDA1/hda6 was significantly lower than that of hda6 mutant. All the results showed that AhHDA1 may down-regulate the expression of cell cycle-related genes through affecting the polar transport of auxin to inhibit cell division and elongation, contributing to repress the growth of primiry root and generation of lateral roots in Arabidopsis thaliana.
AhHDA1 is a histone deacetylase of peanut, which is highly homologous to HDA6 in Arabidopsis thaliana. Both AhHDA1 and AtHDA6 have conserved histone deacetylase activity domain. 7-day-old Col-0, hda6 mutant and AhHDA1/hda6 lines were obtained to analyze the length of primiry root and the number of lateral roots. The results showed that, the growth of primiry root of AhHDA1/hda6 was obviously slowed down and its number of lateral roots also decreased significantly compared to hda6 mutant. The root tip of three lines were observed by Leica DM6 fluorescence microscope, the results revealed that the length of meristem and elongation cells of AhHDA1/hda6 were significantly shortened compared with hda6 mutant, however, it had no significant difference compared with Col-0. Further detection of gene expression levels showed that, the expression of cell cycle-related and auxin polar transport-related genes in root of AhHDA1/hda6 was significantly lower than that of hda6 mutant. All the results showed that AhHDA1 may down-regulate the expression of cell cycle-related genes through affecting the polar transport of auxin to inhibit cell division and elongation, contributing to repress the growth of primiry root and generation of lateral roots in Arabidopsis thaliana.
引文
Balzan S,Johal GS,Carraro N(2014).The role of auxin transporters in monocots development.Front Plant Sci,5:393
Beemster GT,Baskin TI(1998).Analysis of cell division and elongation underlying the developmental acceleration of root growth in Arabidopsis thaliana.Plant Physiol,116(4):1515-1526
Casimiro I,Beeckman T,Graham N,et al(2003).Dissecting Arabidopsis lateral root development.Trends Plant Sci,8(4):165-171
Castro RO,Trujillo MM,Bucio JL,et al(2007).Effects of dichromate on growth and root system architecture of Arabidopsis thaliana seedlings.Plant Sci,172(1):684-691
Chen RQ,Shu W,Ge K,et al(2017).Effect on growth and expressions of stress-related genes in peanut under drought stress training.Plant Physiol J,53(10):1921-1927(in Chinese with English abstract)[陈容钦,舒文,葛奎等(2017).干旱胁迫训练对花生生长及胁迫相关基因表达的影响.植物生理学报,53(10):1921-1927]
Chung PJ,Kim YS,Jeong JS,et al(2010).The histone deacetylase OsHDAC1 epigenetically regulates the OsNAC6 gene that controls seedling root growth in rice.Plant J,59(5):764-776
Deng B,Li L,Li XY,et al(2016).Heterologous expression of AhHDA1 affects drought resistance of Arabidopsis plants.J South China Nor Univ(Nat Sci Edn),48(5):52-57(in Chinese with English abstract)[邓斌,李玲,李晓云等(2016).AhHDA1异源表达影响拟南芥植株干旱性.华南师范大学学报(自然科学版),48(5):52-57]
Fukaki H,Taniguchi N,Tasaka M(2006).PICKLE is required for SOLITARY-ROOT/IAA14-mediated repression of ARF7 and ARF19 activity during Arabidopsis lateral root initiation.Plant J,48(3):380-389
Fukaki H,Tasaka M(2009).Hormone interactions during lateral root formation.Plant Mol Biol,69(4):437-449
Himanen K,Boucheron E,Vanneste S,et al(2002).Auxin-mediated cell cycle activation during early lateral root initiation.Plant Cell,14(10):2339-2351
Huang SY,Feng JX,Chen JY,et al(2018).ABA sensitivity and drought tolerance of Arabidopsis dmard abf3 mutant.Plant Physiol J,54(3):386-392(in Chinese with English abstract)[黄淑颜,冯锦欣,陈家逸等(2018).拟南芥dmard abf3突变体对ABA的敏感性和耐旱性能.植物生理学报,54(3):386-392]
Inahashi H,Shelley IJ,Yamauchi T,et al(2018).OsPIN2,which encodes a member of the auxin efflux carrier proteins,is involved in root elongation growth and lateral root formation patterns via the regulation of auxin distribution in rice.Physiol Plant,164(2):216-225
Jang IC,Pahk YM,Song SI,et al(2010).Structure and expression of the rice class-I type histone deacetylase genes OsHDAC1-3:OsHDAC1 overexpression in transgenic plants leads to increased growth rate and altered architecture.Plant J,33(3):531-541
Kuppusamy KT,Ivashuta S,Bucciarelli B,et al(2009).Knockdown of CELL DIVISION CYCLE16 reveals an inverse relationship between lateral root and nodule numbers and a link to auxin in Medicago truncatula.Plant Physiol,151(3):1155-1166
Nguyen HN,Kim JH,Chan YJ,et al(2013).Inhibition of histone deacetylation alters Arabidopsis root growth in response to auxin via PIN1 degradation.Plant Cell Rep,32(10):1625-1636
Petricka JJ,Winter CM,Benfey PN(2012).Control of Arabidopsis root development.Annu Rev Plant Biol,63:563-590
Sanz L,Dewitte W,Forzani C,et al(2011).The Arabidopsis D-type cyclin CYCD2;1 and the inhibitor ICK2/KRP2modulate auxin-induced lateral root formation.Plant Cell,23(2):641-660
Satbhai SB,Ristova D,Busch W(2015).Underground tuning:quantitative regulation of root growth.J Exp Bot,66(4):1099-1112
Wakeel A,Ali I,Upreti S,et al(2018).Ethylene mediates dichromate induced inhibition of primary root growth by altering AUX1 expression and auxin accumulation in Arabidopsis thaliana.Plant Cell Environ,41(6):1453-1467
Zhang B,Xia DA,Ma XJ(2017).Function of histone deacetylase in poplar root regeneration and growth.Jiangsu Agric Sci,45(5):40-43(in Chinese)[张冰,夏德安,马旭俊(2017).组蛋白去乙酰化酶在杨树根再生和生长中的功能.江苏农业科学,45(5):40-43]
Beemster GT,Baskin TI(1998).Analysis of cell division and elongation underlying the developmental acceleration of root growth in Arabidopsis thaliana.Plant Physiol,116(4):1515-1526
Casimiro I,Beeckman T,Graham N,et al(2003).Dissecting Arabidopsis lateral root development.Trends Plant Sci,8(4):165-171
Castro RO,Trujillo MM,Bucio JL,et al(2007).Effects of dichromate on growth and root system architecture of Arabidopsis thaliana seedlings.Plant Sci,172(1):684-691
Chen RQ,Shu W,Ge K,et al(2017).Effect on growth and expressions of stress-related genes in peanut under drought stress training.Plant Physiol J,53(10):1921-1927(in Chinese with English abstract)[陈容钦,舒文,葛奎等(2017).干旱胁迫训练对花生生长及胁迫相关基因表达的影响.植物生理学报,53(10):1921-1927]
Chung PJ,Kim YS,Jeong JS,et al(2010).The histone deacetylase OsHDAC1 epigenetically regulates the OsNAC6 gene that controls seedling root growth in rice.Plant J,59(5):764-776
Deng B,Li L,Li XY,et al(2016).Heterologous expression of AhHDA1 affects drought resistance of Arabidopsis plants.J South China Nor Univ(Nat Sci Edn),48(5):52-57(in Chinese with English abstract)[邓斌,李玲,李晓云等(2016).AhHDA1异源表达影响拟南芥植株干旱性.华南师范大学学报(自然科学版),48(5):52-57]
Fukaki H,Taniguchi N,Tasaka M(2006).PICKLE is required for SOLITARY-ROOT/IAA14-mediated repression of ARF7 and ARF19 activity during Arabidopsis lateral root initiation.Plant J,48(3):380-389
Fukaki H,Tasaka M(2009).Hormone interactions during lateral root formation.Plant Mol Biol,69(4):437-449
Himanen K,Boucheron E,Vanneste S,et al(2002).Auxin-mediated cell cycle activation during early lateral root initiation.Plant Cell,14(10):2339-2351
Huang SY,Feng JX,Chen JY,et al(2018).ABA sensitivity and drought tolerance of Arabidopsis dmard abf3 mutant.Plant Physiol J,54(3):386-392(in Chinese with English abstract)[黄淑颜,冯锦欣,陈家逸等(2018).拟南芥dmard abf3突变体对ABA的敏感性和耐旱性能.植物生理学报,54(3):386-392]
Inahashi H,Shelley IJ,Yamauchi T,et al(2018).OsPIN2,which encodes a member of the auxin efflux carrier proteins,is involved in root elongation growth and lateral root formation patterns via the regulation of auxin distribution in rice.Physiol Plant,164(2):216-225
Jang IC,Pahk YM,Song SI,et al(2010).Structure and expression of the rice class-I type histone deacetylase genes OsHDAC1-3:OsHDAC1 overexpression in transgenic plants leads to increased growth rate and altered architecture.Plant J,33(3):531-541
Kuppusamy KT,Ivashuta S,Bucciarelli B,et al(2009).Knockdown of CELL DIVISION CYCLE16 reveals an inverse relationship between lateral root and nodule numbers and a link to auxin in Medicago truncatula.Plant Physiol,151(3):1155-1166
Nguyen HN,Kim JH,Chan YJ,et al(2013).Inhibition of histone deacetylation alters Arabidopsis root growth in response to auxin via PIN1 degradation.Plant Cell Rep,32(10):1625-1636
Petricka JJ,Winter CM,Benfey PN(2012).Control of Arabidopsis root development.Annu Rev Plant Biol,63:563-590
Sanz L,Dewitte W,Forzani C,et al(2011).The Arabidopsis D-type cyclin CYCD2;1 and the inhibitor ICK2/KRP2modulate auxin-induced lateral root formation.Plant Cell,23(2):641-660
Satbhai SB,Ristova D,Busch W(2015).Underground tuning:quantitative regulation of root growth.J Exp Bot,66(4):1099-1112
Wakeel A,Ali I,Upreti S,et al(2018).Ethylene mediates dichromate induced inhibition of primary root growth by altering AUX1 expression and auxin accumulation in Arabidopsis thaliana.Plant Cell Environ,41(6):1453-1467
Zhang B,Xia DA,Ma XJ(2017).Function of histone deacetylase in poplar root regeneration and growth.Jiangsu Agric Sci,45(5):40-43(in Chinese)[张冰,夏德安,马旭俊(2017).组蛋白去乙酰化酶在杨树根再生和生长中的功能.江苏农业科学,45(5):40-43]