系统性红斑狼疮患者血清LncRNA MIR155HG的表达及临床意义
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摘要
目的探讨LncRNA MIR155HG在系统性红斑狼疮患者临床诊治中的价值。方法采用反转录PCR法(RT-PCR)检测80例2016年1月至2017年12月来我院门诊和住院就诊的系统性红斑狼疮(SLE)患者及40例同期来我院健康体检的对照者的血清LncRNA MIR155HG的表达,分析其表达与临床组患者血清LncRNA MIR155HG的表达水平显著增高,SLE活动期患者组高于SLE稳定期患者,差异均有统计学意义(P <0.001)。LncRNA MIR155HG的表达与系统性红斑狼疮疾病活动度(SLEDAI)呈正相关,差异均有统计学意义(P <0.001)。SLE合并狼疮肾炎患者血清LncRNA MIR155HG的表达明显高于无肾炎组,差异均有统计学意义(P <0.05)。血清LncRNAMIR155HG作为诊断SLE患者血清标志物的ROC灵敏度和特异性分别为81.3%(阈值15%)和90.2%(阈值30%)。血清LncRNA MIR155HG作为诊断是否合并狼疮肾炎的ROC曲线下面积为0.915(95%CI:0.870～0.960;P <0.001),灵敏度和特异性分别为82.5%(阈值20%)和90.8%(阈值30%)。结论血清LncRNA MIR155HG在SLE患者中高表达,与SLE患者的活动度相关,LncRNA MIR155HG有可能成为一个新的SLE疾病活动度评估及预后判断的分子标志物。
Objective To explore the value of LncRNA MIR155HG in the diagnosis and treatment of patients with systemic lupus erythematosus.Methods Eighty patients with systemic lupus erythematosus(SLE)and40 cases of healthy controls from January 2016 to December 2017 were involved in the study.The expression of serum LncRNA MIR155HG in the 2 groups was detected by reverse transcriptional PCR(RT-PCR),and its association with the clinicopathological features was analyzed.Results Compared with that in the control group,the expression level of serum LncRNA MIR155HG in the SLE group was significantly increased.The expression was higher in patients with active SLE than that in those with stable SLE(P<0.001).The expression of LncRNA MIR155HG was positively correlated with disease activity in systemic lupus erythematosus(SLEDAI)and the difference was statistically significant(P<0.001).The expression of LncRNA MIR155HG in patients with lupus nephritis combined with SLE was significantly higher than that in patients without nephritis(P<0.05).The area under the receiver operating characteristic curves of serum LncRNA MIR155HG as a serum marker for the diagnosis of SLE was 0.720(95%CI,0.601～0.840;P<0.001),and the sensitivity and specificity were 81.3%(threshold 15%)and 90.2%(threshold 30%),respectively.The area under the ROC curve of serum LncRNA MIR155HG as a diagnosis of lupus nephritis was 0.915(95%CI,0.870～0.960,P<0.001),and its sensitivity and specificity were 82.5%(threshold20%)and 90.8%(threshold 30%),respectively.ConclusionSerum LncRNA MIR155HG is highly expressed in SLE patients and is related to the activity of SLE patients.LncRNA MIR155HG may be a new molecular marker for evaluating the activity of SLE disease and predicting the prognosis.
systemic lupus erythematosusserum LncRNA MIR155HGclinical significance
Objective To explore the value of LncRNA MIR155HG in the diagnosis and treatment of patients with systemic lupus erythematosus.Methods Eighty patients with systemic lupus erythematosus(SLE)and40 cases of healthy controls from January 2016 to December 2017 were involved in the study.The expression of serum LncRNA MIR155HG in the 2 groups was detected by reverse transcriptional PCR(RT-PCR),and its association with the clinicopathological features was analyzed.Results Compared with that in the control group,the expression level of serum LncRNA MIR155HG in the SLE group was significantly increased.The expression was higher in patients with active SLE than that in those with stable SLE(P<0.001).The expression of LncRNA MIR155HG was positively correlated with disease activity in systemic lupus erythematosus(SLEDAI)and the difference was statistically significant(P<0.001).The expression of LncRNA MIR155HG in patients with lupus nephritis combined with SLE was significantly higher than that in patients without nephritis(P<0.05).The area under the receiver operating characteristic curves of serum LncRNA MIR155HG as a serum marker for the diagnosis of SLE was 0.720(95%CI,0.601～0.840;P<0.001),and the sensitivity and specificity were 81.3%(threshold 15%)and 90.2%(threshold 30%),respectively.The area under the ROC curve of serum LncRNA MIR155HG as a diagnosis of lupus nephritis was 0.915(95%CI,0.870～0.960,P<0.001),and its sensitivity and specificity were 82.5%(threshold20%)and 90.8%(threshold 30%),respectively.ConclusionSerum LncRNA MIR155HG is highly expressed in SLE patients and is related to the activity of SLE patients.LncRNA MIR155HG may be a new molecular marker for evaluating the activity of SLE disease and predicting the prognosis.
systemic lupus erythematosusserum LncRNA MIR155HGclinical significance
引文
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[7]SAHEBARI M,REZAIEYAZDI Z,KHODASHAHI M,et al.Brain single photon emission computed tomography scan(SPECT)and functional MRI in systemic lupus erythematosus patients with cognitive dysfunction:A systematic review[J].Asia Ocean J Nucl Med Biol,2018,6(2):97-107.
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[9]JI K,FAN R,ZHANG J,et al.Long non-coding RNA expression profile in Cdk5-knockdown mouse skin[J].Gene,2018,6(2):87-95.
[10]RODRIGUEZ B R,ORTEGA G A,HIDALGO M A,et al.Long non-coding RNAs:implications in targeted diagnoses,prognosis,and improved therapeutic strategies in human non-and triple-negative breast cancer[J].Clin Epigenetics,2018,10:88.
[11]WANG Y,CHEN S,CHEN S,et al.Long noncoding RNA expression profile and association with SLEDAI score in monocytederived dendritic cells from patients with systematic lupus erythematosus[J].Arthritis Res Ther,2018,20(1):138.
[12]XU Y,DENG W,AND ZHANG W.Long non-coding RNATUG1 protects renal tubular epithelial cells against injury induced by lipopolysaccharide via regulating microRNA-223[J].Biomed Pharmacother,2018,104:509-519.
[13]WU X,WANG Y,YU T,et al.Blocking MIR155HG/miR-155axis inhibits mesenchymal transition in glioma[J].Neuro Oncol,2017,19(9):1195-1205.
[14]WANG G,TAM L S,KWAN B C,et al.Expression of miR-146a and miR-155 in the urinary sediment of systemic lupus erythematosus[J].Clin Rheumatol,2012,31(3):435-440.
[2]王晓东,陈冬莹,田媛媛,等.系统性红斑狼疮患者胎儿脐动脉血流彩色多普勒超声对早产的监测作用[J].实用医学杂志,2018,34(4):572-575.
[3]BALASUBRAMANIYAN V AND BHAT K P.Targeting MIR155HG in glioma:A novel approach[J].Neuro Oncol,2017,19(9):1152-1153.
[4]BAYTAK E,GONG Q,AKMAN B,et al.Whole transcriptome analysis reveals dysregulated oncogenic lncRNAs in natural killer/T-cell lymphoma and establishes MIR155HG as a target of PRDM1[J].Tumour Biol,2017,39(5):1010428317701648.
[5]MORMAN R E,SCHWEICKERT P G,KONIECZNY S F,et al.BATF regulates the expression of Nfil3,Wnt10a and miR155hg for efficient induction of antibody class switch recombination in mice[J].Eur J Immunol,2018.
[6]NASONOV E,SOLOVIEV S,DAVIDSON J E,et al.Systemic lupus erythematosus and associated healthcare resource consumption in selected cities from the Russian Federation,Republic of Kazakhstan and Ukraine:The ESSENCE study[J].J Med Econ,2018:1-22.
[7]SAHEBARI M,REZAIEYAZDI Z,KHODASHAHI M,et al.Brain single photon emission computed tomography scan(SPECT)and functional MRI in systemic lupus erythematosus patients with cognitive dysfunction:A systematic review[J].Asia Ocean J Nucl Med Biol,2018,6(2):97-107.
[8]DANG Y,WEI X,XUE L,et al.Long non-coding RNA in glioma:Target miRNA and signaling pathways[J].Clin Lab,2018,64(6):887-894.
[9]JI K,FAN R,ZHANG J,et al.Long non-coding RNA expression profile in Cdk5-knockdown mouse skin[J].Gene,2018,6(2):87-95.
[10]RODRIGUEZ B R,ORTEGA G A,HIDALGO M A,et al.Long non-coding RNAs:implications in targeted diagnoses,prognosis,and improved therapeutic strategies in human non-and triple-negative breast cancer[J].Clin Epigenetics,2018,10:88.
[11]WANG Y,CHEN S,CHEN S,et al.Long noncoding RNA expression profile and association with SLEDAI score in monocytederived dendritic cells from patients with systematic lupus erythematosus[J].Arthritis Res Ther,2018,20(1):138.
[12]XU Y,DENG W,AND ZHANG W.Long non-coding RNATUG1 protects renal tubular epithelial cells against injury induced by lipopolysaccharide via regulating microRNA-223[J].Biomed Pharmacother,2018,104:509-519.
[13]WU X,WANG Y,YU T,et al.Blocking MIR155HG/miR-155axis inhibits mesenchymal transition in glioma[J].Neuro Oncol,2017,19(9):1195-1205.
[14]WANG G,TAM L S,KWAN B C,et al.Expression of miR-146a and miR-155 in the urinary sediment of systemic lupus erythematosus[J].Clin Rheumatol,2012,31(3):435-440.