便携式光纤拉曼胃癌检测仪的研发和应用
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摘要
目的研发一种便携式光纤拉曼胃癌检测仪,探讨该设备对胃癌检测的可行性。方法便携式光纤拉曼胃癌检测仪的主要组件包括785 nm半导体激光器、光纤探头、微型光谱仪。利用该设备对正常胃粘膜组织和胃癌组织进行拉曼光谱检测,并与共聚焦显微拉曼光谱仪获取的光谱数据进行对比。结果便携式光纤拉曼胃癌检测仪能够采集到完整的正常胃粘膜组织和胃癌组织的光谱,与共聚焦显微拉曼光谱仪获取的光谱相差不大。结论该设备可以完整呈现不同胃黏膜组织的拉曼光谱,可用于胃癌的鉴别,为研制拉曼光谱胃镜提供了理论基础。
Objective To design a portable fiber Raman gastric cancer detector, and investigate its feasibility in the detection of gastric cancer. Methods The main components of the portable fiber Raman gastric cancer detector include a 785 nm semiconductor laser, a fiber optic probe, and a miniature spectrometer. The Raman spectra of normal gastric mucosa tissues and gastric cancer tissues were detected by the portable fiber Raman gastric cancer detector, and were compared with the spectral data obtained by confocal microraman spectrometer. Results The complete spectrum of normal gastric mucosa tissues and gastric cancer tissues could be collected by the portable fiber Raman gastric cancer detector, which had no significant difference from the spectrum obtained by confocal microraman spectrometer. Conclusion This detector could completely present the Raman spectrum of different gastric mucosa tissues, and could be used for the identification of gastric cancer, providing a theoretical basis for the development of Raman spectroscopic gastroscopy.
Objective To design a portable fiber Raman gastric cancer detector, and investigate its feasibility in the detection of gastric cancer. Methods The main components of the portable fiber Raman gastric cancer detector include a 785 nm semiconductor laser, a fiber optic probe, and a miniature spectrometer. The Raman spectra of normal gastric mucosa tissues and gastric cancer tissues were detected by the portable fiber Raman gastric cancer detector, and were compared with the spectral data obtained by confocal microraman spectrometer. Results The complete spectrum of normal gastric mucosa tissues and gastric cancer tissues could be collected by the portable fiber Raman gastric cancer detector, which had no significant difference from the spectrum obtained by confocal microraman spectrometer. Conclusion This detector could completely present the Raman spectrum of different gastric mucosa tissues, and could be used for the identification of gastric cancer, providing a theoretical basis for the development of Raman spectroscopic gastroscopy.
引文
[1]黄雯雅,金晖,刘璇,等.拉曼光谱技术在医学诊断中的研究进展[J].东南大学学报(医学版),2017,(6):1031-1035.
[2]欧阳俊辉,毛华.拉曼光谱技术在胃癌诊断中的应用[J].胃肠病学,2013,18(6):368-370.
[3]Yan XL,Dong RX,Zhang L,et al.Raman spectra of single cell from gastrointestinal cancer patients[J].World J Gastroenter ol,2005,11(21):3290-3292.
[4]金少琴,毛华.近红外线激光拉曼光谱具有在分子水平诊断胃癌的价值[J].南方医科大学学报,2014,(3):391-395.
[5]马君,徐明,巩龙静,等.近红外激光拉曼技术在体探测胃癌腹膜播散[J].中国激光,2011,(9):215-220.
[6]刘燕玲,黄丽韫,钟会清,等.表面增强拉曼光谱在胃癌诊断和分期中的应用[J].世界华人消化杂志,2018,(18):1102-1110.
[7]Colagar AH,Chaichi MJ,Khadjvand T.Fourier transform infrared microspectroscopy as a diagnostic tool for distinguishing between normal and malignant human gastric tissue[J].JBiosci,2011,36(4):669-677.
[8]王巍,潘志峰,唐伟跃,等.胃癌患者血红蛋白的表面增强拉曼光谱分析[J].光谱学与光谱分析,2015,(12):3402-3405.
[9]易伟松,钱辉跃,江厚敏.光纤和显微拉曼光谱结合化学计量学鉴别胃癌组织[J].中国医学物理学杂志,2016,33(2):134-140
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[11]高凤,刘文芳,孟子晖,等.激光拉曼光谱技术在火炸药分析检测中的应用研究进展[J].含能材料,2018,(2):185-196.
[12]隋成华,屠潜,鄢波,等.拉曼光谱在新型毒品快速检测中的应用[J].光谱学与光谱分析,2018,(11):3395-3400.
[13]滕官宏伟,蒋晗,张雷蕾,等.便携式鱼肉抗菌药物残留量拉曼检测系统设计研究[J].中国计量大学学报,2017,(4):442-447.
[14]罗曦芸,叶菲,吴来明,等.便携式拉曼光谱用于文物及文物保护材料光老化作用的快速评价[J].光谱学与光谱分析,2010,(9):2405-2408
[15]任小娟,温宝英,陈鉴东,等.便携式拉曼光谱仪快速检测废水中残留有机溶剂[J].光散射学报,2018,(3):258-263.
[16]李芳,陆安祥,王纪华.拉曼光谱在粮食检测中的应用[J].食品安全质量检测学报,2016,(11):4408-4414.
[17]董金颖,张阳,刘皓,等.拉曼光谱技术在病原微生物快速检测中的应用与展望[J].中华检验医学杂志,2018,41(1):5-8.
[18]李莉莉,赵丽娇,钟儒刚.拉曼光谱检测生物大分子损伤的研究进展[J].光谱学与光谱分析,2012,32(9):2422-2426.
[19]Mccreery RL,Fleischmann M,Hendra P.Fiber optic probe for remote Raman spectrometry[J].Anal Chem,1983,55(1):146-148.
[20]Katagiri T,Sato H,Komachi Y,et al.Improvement and analysis of a micro Raman probe[J].Appl Opt,2009,48(9):1683-1696.
[21]De Lima CJ,Sathaiah S,Silveira L,et al.Development of catheters with low fiber background signals for Raman spectroscopic diagnosis applications[J].Artif Organs,2000,24(3):231-234.
[22]Bergholt MS,Zheng W,Ho KY,et al.Fiber-optic Raman spectroscopy probes gastric carcinogenesis in vivo at endoscopy[J].J Biophotonics,2013,6(1):49-59.
[23]Wilson BC,Marple E,Shim MG,et al.Study of Fiber-optic probes for in vivo medical Raman spectroscopy[J].Appl Spectrosc,1999,53(6):619-627.
[24]Wang J,Lin K,Zheng W,et al.Comparative study of the endoscope-based bevelled and volume fiber-optic Raman probes for optical diagnosis of gastric dysplasia in vivo at endoscopy[J].Anal Bioanal Chem,2015,407(27):8303-8310.
[25]常敏,张久聪,周琴,等.胃癌流行病学研究进展[J].胃肠病学和肝病学杂志,2017,26(9):966-969.
[2]欧阳俊辉,毛华.拉曼光谱技术在胃癌诊断中的应用[J].胃肠病学,2013,18(6):368-370.
[3]Yan XL,Dong RX,Zhang L,et al.Raman spectra of single cell from gastrointestinal cancer patients[J].World J Gastroenter ol,2005,11(21):3290-3292.
[4]金少琴,毛华.近红外线激光拉曼光谱具有在分子水平诊断胃癌的价值[J].南方医科大学学报,2014,(3):391-395.
[5]马君,徐明,巩龙静,等.近红外激光拉曼技术在体探测胃癌腹膜播散[J].中国激光,2011,(9):215-220.
[6]刘燕玲,黄丽韫,钟会清,等.表面增强拉曼光谱在胃癌诊断和分期中的应用[J].世界华人消化杂志,2018,(18):1102-1110.
[7]Colagar AH,Chaichi MJ,Khadjvand T.Fourier transform infrared microspectroscopy as a diagnostic tool for distinguishing between normal and malignant human gastric tissue[J].JBiosci,2011,36(4):669-677.
[8]王巍,潘志峰,唐伟跃,等.胃癌患者血红蛋白的表面增强拉曼光谱分析[J].光谱学与光谱分析,2015,(12):3402-3405.
[9]易伟松,钱辉跃,江厚敏.光纤和显微拉曼光谱结合化学计量学鉴别胃癌组织[J].中国医学物理学杂志,2016,33(2):134-140
[10]高延甲,尹利辉,焦建东,等.便携式拉曼光谱仪无损测定玻璃瓶装液体制剂的影响因素探讨[J].药物分析杂志,2017,(2):358-361.
[11]高凤,刘文芳,孟子晖,等.激光拉曼光谱技术在火炸药分析检测中的应用研究进展[J].含能材料,2018,(2):185-196.
[12]隋成华,屠潜,鄢波,等.拉曼光谱在新型毒品快速检测中的应用[J].光谱学与光谱分析,2018,(11):3395-3400.
[13]滕官宏伟,蒋晗,张雷蕾,等.便携式鱼肉抗菌药物残留量拉曼检测系统设计研究[J].中国计量大学学报,2017,(4):442-447.
[14]罗曦芸,叶菲,吴来明,等.便携式拉曼光谱用于文物及文物保护材料光老化作用的快速评价[J].光谱学与光谱分析,2010,(9):2405-2408
[15]任小娟,温宝英,陈鉴东,等.便携式拉曼光谱仪快速检测废水中残留有机溶剂[J].光散射学报,2018,(3):258-263.
[16]李芳,陆安祥,王纪华.拉曼光谱在粮食检测中的应用[J].食品安全质量检测学报,2016,(11):4408-4414.
[17]董金颖,张阳,刘皓,等.拉曼光谱技术在病原微生物快速检测中的应用与展望[J].中华检验医学杂志,2018,41(1):5-8.
[18]李莉莉,赵丽娇,钟儒刚.拉曼光谱检测生物大分子损伤的研究进展[J].光谱学与光谱分析,2012,32(9):2422-2426.
[19]Mccreery RL,Fleischmann M,Hendra P.Fiber optic probe for remote Raman spectrometry[J].Anal Chem,1983,55(1):146-148.
[20]Katagiri T,Sato H,Komachi Y,et al.Improvement and analysis of a micro Raman probe[J].Appl Opt,2009,48(9):1683-1696.
[21]De Lima CJ,Sathaiah S,Silveira L,et al.Development of catheters with low fiber background signals for Raman spectroscopic diagnosis applications[J].Artif Organs,2000,24(3):231-234.
[22]Bergholt MS,Zheng W,Ho KY,et al.Fiber-optic Raman spectroscopy probes gastric carcinogenesis in vivo at endoscopy[J].J Biophotonics,2013,6(1):49-59.
[23]Wilson BC,Marple E,Shim MG,et al.Study of Fiber-optic probes for in vivo medical Raman spectroscopy[J].Appl Spectrosc,1999,53(6):619-627.
[24]Wang J,Lin K,Zheng W,et al.Comparative study of the endoscope-based bevelled and volume fiber-optic Raman probes for optical diagnosis of gastric dysplasia in vivo at endoscopy[J].Anal Bioanal Chem,2015,407(27):8303-8310.
[25]常敏,张久聪,周琴,等.胃癌流行病学研究进展[J].胃肠病学和肝病学杂志,2017,26(9):966-969.