不同树龄银杏叶内生真菌的多样性及群落结构分析
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摘要
以湖南省壶瓶山国家级自然保护区生长的9株(3组)不同树龄银杏为研究对象,应用IlluminaHiSeq高通量测序技术,分析银杏叶片组织内生真菌的多样性及群落结构。结果表明:银杏叶片内生真菌群落的多样性、群落结构与组成均存在显著性差异,且随着树龄的增加,叶片内生真菌群落结构差异增大;子囊菌门(Ascomycota)为绝对优势菌门;曲霉属(Aspergillus)、假丝酵母属(Candida)、球腔菌属(Mycosphaerella)等真菌为银杏叶内生真菌群落优势菌属;通过对银杏叶黄酮含量进行检测,发现不同树龄银杏叶的黄酮含量存在显著差异,且随着树龄的增加,3种黄酮醇苷(槲皮素、山奈酚和异鼠李素)含量均呈递减趋势;进一步分析表明,黄酮类化合物作为重要环境因子,可能通过影响优势菌门子囊菌中优势菌属的定殖与生长对内生真菌群落的形成与变化产生显著影响。
In this study, using the, Illumina HiSeq high-throughput sequencing technology, we investigated the diversity and structure of endophytic fungal communities harbored in the Ginkgo leaves collected from nine(three groups) ancient Ginkgo with different ages from Hupingshan National Nature Reserve, Hunan Province. The result showed that the endophytic fung diversity, structure and composition were significantly different. The differences of endophytic fungal structure increased with the ages of the studied Ginkgo. Ascomycota was the dominant phyla and Aspergillus, Candida, Mycosphaerella were the dominant genus.The determination of flavonoids in Ginkgo leaves found that the contents of three flavonol glycosides(quercetin, kaempferol and isorhamnetin)decreased when the growth years increased. It concluded that, as an important environmental factor, the flavonoids in Ginkgo leaves had a significant effect on the endophytic fungal communities possibly through impacting the colonization and growth of the dominant Ascomycota genera.
In this study, using the, Illumina HiSeq high-throughput sequencing technology, we investigated the diversity and structure of endophytic fungal communities harbored in the Ginkgo leaves collected from nine(three groups) ancient Ginkgo with different ages from Hupingshan National Nature Reserve, Hunan Province. The result showed that the endophytic fung diversity, structure and composition were significantly different. The differences of endophytic fungal structure increased with the ages of the studied Ginkgo. Ascomycota was the dominant phyla and Aspergillus, Candida, Mycosphaerella were the dominant genus.The determination of flavonoids in Ginkgo leaves found that the contents of three flavonol glycosides(quercetin, kaempferol and isorhamnetin)decreased when the growth years increased. It concluded that, as an important environmental factor, the flavonoids in Ginkgo leaves had a significant effect on the endophytic fungal communities possibly through impacting the colonization and growth of the dominant Ascomycota genera.
引文
[1]刘艳武.南蛇藤叶和银杏叶粗提物对蛞蝓的药效[J].安徽农业科学,2011,39(31):19176,19185.
[2]杜素娟,郭晓恒.植物内生真菌对植物次生代谢产物的影响[J].现代农业科学,2009(5):17-18.
[3]王剑文,郑丽屏,谭仁祥.促进黄花蒿发根青蒿素合成的内生真菌诱导子的制备[J].生物工程学报,2006,22(5):829-834.
[4]CHAPLA V M,BIASETTO C R,ARAUJO A R.Endophytic fungi:an unexplored and sustainable source of new and bioactive natural products[J].Revista Virtual De Química,2013,5(3):421-437.
[5]QIU M,XIE RS,SHI Y,et al.Isolation and identification of two flavonoid-producing endophytic fungi from Ginkgo biloba L.[J].Annals of Microbiology,2010,60(1):143-150.
[6]国家药典委员会.中华人民共和国药典[M].北京:中国医药科技出版社,2015.
[7]甘金莲,陈晔,刘瑜琦,等.银杏内生真菌的生态分布[J].菌物研究,2007,5(3):137-141.
[8]杜少康,陈双林,林岱,等.银杏叶部内生真菌多样性的研究[J].菌物学报,2009,28(4):504-511.
[9]HOFFMAN M T,ARNOLD A E.Geographic locality and host identity shape fungal endophyte communities in cupressaceous trees[J].Mycological Research,2008,112(3):331-344.
[10]JOSHEE S,PAULUS B C,PARK D,et al.Diversity and distribution of fungal foliar endophytes in New Zealand Podocarpaceae[J].Mycological Research,2009,113(9):1003-1015.
[11]GUO L D,HUANG G R,WANG Y.Seasonal and tissue age influences on endophytic fungi of Pinus tabulaeformis(Pinaceae)in the Dongling Mountains,Beijing[J].Journal of Integrative Plant Biology,2008,50(8):997-1003.
[12]FAETH S H,FAGAN W F.Fungal endophytes:common host plant symbionts but uncommon mutualists[J].Integrative and Comparative Biology,2002,42(2):360-368.
[13]江曙,段金廒,陶金华,等.明党参内生真菌种群的生态分布及其诱导子活性研究[J].中草药,2010,41(1):121-125.
[14]WU L S,HAN T,LI W C,et al.Geographic and tissue influences on endophytic fungal communities of taxus chinensis var.mairei in China[J].Current Microbiology,2013,66(1):40-48.
[15]周凯凯,张胜,赵忠.不同树龄银杏叶片差异蛋白组学研究[J].西北林学院学报,2018,33(1):105-112.
[16]ZHANG J Y,ZHANG N,LIU Y X,et al.Root microbiota shift in rice correlates with resident time in the field and developmental stage[J].Science China Life Sciences,2018,61(6):613-621.
[17]YATSUNENKO T,REY F E,MANARY M J,et al.Human gut microbiome viewed across age and geography[J].Nature,2012,486:222-227.
[18]AOKI T,AKASHI T,AYABE S I.Flavonoids of leguminous plants:structure,biological activity,and biosynthesis[J].Journal of Plant Research,2000,113(4):475-488.
[19]TAGHAVI S,GARAFOLA C,MONCHY S,et al.Genome survey and characterization of endophytic bacteria exhibiting a beneficial effect on growth and development of poplar trees[J].Applied and Environmental Microbiology,2009,75(3):748-757.
[20]SENGUPTA A,CHAUDHURI S.Arbuscular mycorrhizal relations of mangrove plant community at the Ganges river estuary in India[J].Mycorrhiza,2002,12(4):169-174.
[2]杜素娟,郭晓恒.植物内生真菌对植物次生代谢产物的影响[J].现代农业科学,2009(5):17-18.
[3]王剑文,郑丽屏,谭仁祥.促进黄花蒿发根青蒿素合成的内生真菌诱导子的制备[J].生物工程学报,2006,22(5):829-834.
[4]CHAPLA V M,BIASETTO C R,ARAUJO A R.Endophytic fungi:an unexplored and sustainable source of new and bioactive natural products[J].Revista Virtual De Química,2013,5(3):421-437.
[5]QIU M,XIE RS,SHI Y,et al.Isolation and identification of two flavonoid-producing endophytic fungi from Ginkgo biloba L.[J].Annals of Microbiology,2010,60(1):143-150.
[6]国家药典委员会.中华人民共和国药典[M].北京:中国医药科技出版社,2015.
[7]甘金莲,陈晔,刘瑜琦,等.银杏内生真菌的生态分布[J].菌物研究,2007,5(3):137-141.
[8]杜少康,陈双林,林岱,等.银杏叶部内生真菌多样性的研究[J].菌物学报,2009,28(4):504-511.
[9]HOFFMAN M T,ARNOLD A E.Geographic locality and host identity shape fungal endophyte communities in cupressaceous trees[J].Mycological Research,2008,112(3):331-344.
[10]JOSHEE S,PAULUS B C,PARK D,et al.Diversity and distribution of fungal foliar endophytes in New Zealand Podocarpaceae[J].Mycological Research,2009,113(9):1003-1015.
[11]GUO L D,HUANG G R,WANG Y.Seasonal and tissue age influences on endophytic fungi of Pinus tabulaeformis(Pinaceae)in the Dongling Mountains,Beijing[J].Journal of Integrative Plant Biology,2008,50(8):997-1003.
[12]FAETH S H,FAGAN W F.Fungal endophytes:common host plant symbionts but uncommon mutualists[J].Integrative and Comparative Biology,2002,42(2):360-368.
[13]江曙,段金廒,陶金华,等.明党参内生真菌种群的生态分布及其诱导子活性研究[J].中草药,2010,41(1):121-125.
[14]WU L S,HAN T,LI W C,et al.Geographic and tissue influences on endophytic fungal communities of taxus chinensis var.mairei in China[J].Current Microbiology,2013,66(1):40-48.
[15]周凯凯,张胜,赵忠.不同树龄银杏叶片差异蛋白组学研究[J].西北林学院学报,2018,33(1):105-112.
[16]ZHANG J Y,ZHANG N,LIU Y X,et al.Root microbiota shift in rice correlates with resident time in the field and developmental stage[J].Science China Life Sciences,2018,61(6):613-621.
[17]YATSUNENKO T,REY F E,MANARY M J,et al.Human gut microbiome viewed across age and geography[J].Nature,2012,486:222-227.
[18]AOKI T,AKASHI T,AYABE S I.Flavonoids of leguminous plants:structure,biological activity,and biosynthesis[J].Journal of Plant Research,2000,113(4):475-488.
[19]TAGHAVI S,GARAFOLA C,MONCHY S,et al.Genome survey and characterization of endophytic bacteria exhibiting a beneficial effect on growth and development of poplar trees[J].Applied and Environmental Microbiology,2009,75(3):748-757.
[20]SENGUPTA A,CHAUDHURI S.Arbuscular mycorrhizal relations of mangrove plant community at the Ganges river estuary in India[J].Mycorrhiza,2002,12(4):169-174.