Local conformation transitions of linear DNA induced by cisplatin
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摘要
Cisplatin is the most successful anti-tumor drug,and its pharmacological property is generally considered to derive from the modification of DNA molecules.Structural modifications of short DNA induced by cisplatin have already been investigated.However,the conformation transitions induced by cisplatin are not clear.In the present letter,we have studied the effect of low-concentration cisplatin on DNA conformation by using AFM imaging.We observed formations of micro-rod structures of linear DNA induced by cisplatin.A method is presented to quantitatively analyze the occurrence of micro-rod structures.We found that the formation of micro-rod structures depends on the DNA sequence.Based on the results,we proposed a physical mechanism to explain the local conformation transitions of DNA molecules under the influence of cisplatin.
Cisplatin is the most successful anti-tumor drug,and its pharmacological property is generally considered to derive from the modification of DNA molecules.Structural modifications of short DNA induced by cisplatin have already been investigated.However,the conformation transitions induced by cisplatin are not clear.In the present letter,we have studied the effect of low-concentration cisplatin on DNA conformation by using AFM imaging.We observed formations of micro-rod structures of linear DNA induced by cisplatin.A method is presented to quantitatively analyze the occurrence of micro-rod structures.We found that the formation of micro-rod structures depends on the DNA sequence.Based on the results,we proposed a physical mechanism to explain the local conformation transitions of DNA molecules under the influence of cisplatin.
Cisplatin is the most successful anti-tumor drug,and its pharmacological property is generally considered to derive from the modification of DNA molecules.Structural modifications of short DNA induced by cisplatin have already been investigated.However,the conformation transitions induced by cisplatin are not clear.In the present letter,we have studied the effect of low-concentration cisplatin on DNA conformation by using AFM imaging.We observed formations of micro-rod structures of linear DNA induced by cisplatin.A method is presented to quantitatively analyze the occurrence of micro-rod structures.We found that the formation of micro-rod structures depends on the DNA sequence.Based on the results,we proposed a physical mechanism to explain the local conformation transitions of DNA molecules under the influence of cisplatin.
引文
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20 .Luckel F,Kubo K,Tsumoto K et al(2005)FEBS Lett579 :5119–5122
21 .Yang H,Metera KL,Sleiman HF(2010)Coord Chem Rev254 :2403–2415
2 .Bruhn SL,Toney JH,Lippard SJ(1990)Prog Inorg Chem38 :477–516
3 .Johnson NP,Hoeschele JD,Rahn RO(1980)Chem Biol Interact30 :151–169
4 .Bancroft DP,Lepre CA,Lippard SJ(1990)J Am Chem Soc112 :6860–6871
5 .Vaisman A,Chaney SG(2000)J Biol Chem 275:13017–13025
6 .Jung YW,Lippard SJ(2003)J Biol Chem 278:52084–52092
7 .Takahara PM,Rosenzweig AC,Frederick CA et al(1995)Nature377 :649–652
8 .Takahara PM,Frederick CA,Lippard SJ(1996)J Am Chem Soc118 :12309–12321
9 .Gelasco A,Lippard SJ(1998)Biochemistry 37:9230–9239
10 .Stehlikova K,Kostrhunova H,Kasparkova J et al(2002)Nucleic Acids Res 30:2894–2898
11 .Jamieson ER,Lippard SJ(1999)Chem Rev 99:2467–2498
12 .Hou XM,Zhang XH,Wei KJ et al(2009)Nucleic Acids Res37 :1400–1410
13 .Ji C,Zhang L,Dou S et al(2011)Acta Phys Sin 60:098703
14 .Zuccheri G,Dame RT,Aquila M et al(1998)Appl Phys A66 :S585–S589
15 .Zhang XH,Hou XM,Ji C et al(2009)Chin Sci Bull54 :2425–2433
16 .Rivetti C,Guthold M,Bustamante C(1996)J Mol Biol264 :919–932
17 .Garcia R,Perez R(2002)Surf Sci Rep 47:197–301
18 .Berge T,Jenkins NS,Hopkirk RB et al(2002)Nucleic Acids Res30 :2980–2986
19 .Jung YW,Lippard SJ(2007)Chem Rev 107:1387–1407
20 .Luckel F,Kubo K,Tsumoto K et al(2005)FEBS Lett579 :5119–5122
21 .Yang H,Metera KL,Sleiman HF(2010)Coord Chem Rev254 :2403–2415