HPLC-DAD法同时测定降压类保健食品中非法添加的12种化学成分
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摘要
目的:以HPLC法同时测定保健食品中非法添加的阿替洛尔、盐酸可乐定、氢氯噻嗪、盐酸哌唑嗪、卡托普利、利血平、硝苯地平、氨氯地平、尼群地平、尼莫地平、非洛地平和尼索地平。方法:HPLC法,采用Agilent Extend C18(4.6 mm×250 mm,5μm)色谱柱,以乙腈-0.2%磷酸溶液为流动相,梯度洗脱;流速:1.0 mL·min~(-1);柱温:35℃;检测波长:220 nm。结果:上述12个成分在各自线性范围内均线性良好,r均>0.996 9。平均回收率为94.8%～103.1%,RSD为0.89%～1.7%(n=9)。结论:此法简便、准确、重复性好,可同时测定降压类保健食品中非法添加的12种化学成分。
Objective:To establish an HPLC method for simultaneous detection of atenolol,clonidine hydrochlorid,hydrochorothiazide,captopril,reserpine,nifedipine,amlodipine,nitrendipine,nimodipine,felodipine and nisoldipine,which were illegally added into health foods.Methods:The HPLC method was performed on a Agilent Extend C18column(4.6 mm × 250 mm,5 μm).The column temperature was 35 ℃.The mobile phase was acetonitrile-0.2% phosphate acid at a flow rate of 1.0 mL·min-1.The determination wavelength was 220 nm.Results:All components had good linearities in their own linear ranges.The average recoveries were 94.8%-103.1%,RSD was 0.89%-1.7%(n=9).Conclusion:The method is simple,reliable and reproducible,which can be used to detect 12 anti-hypertensive chemical components in health foods.
Objective:To establish an HPLC method for simultaneous detection of atenolol,clonidine hydrochlorid,hydrochorothiazide,captopril,reserpine,nifedipine,amlodipine,nitrendipine,nimodipine,felodipine and nisoldipine,which were illegally added into health foods.Methods:The HPLC method was performed on a Agilent Extend C18column(4.6 mm × 250 mm,5 μm).The column temperature was 35 ℃.The mobile phase was acetonitrile-0.2% phosphate acid at a flow rate of 1.0 mL·min-1.The determination wavelength was 220 nm.Results:All components had good linearities in their own linear ranges.The average recoveries were 94.8%-103.1%,RSD was 0.89%-1.7%(n=9).Conclusion:The method is simple,reliable and reproducible,which can be used to detect 12 anti-hypertensive chemical components in health foods.
引文
[1]王静文.保健食品中非法添加物检测方法的研究与建立[D].中国食品药品检定研究院,2014.
[2]郭宝平.保健食品中非法添加药物的检测方法的研究[J].天津药学,2013,25(6):62-4.
[3]吴公平,雷玉萍,黎银波,等.辅助降压类保健品中非法添加化学药品的快速筛选方法[J].中国药业,2010,19(14):42-3.
[4]郑荣,王柯,季申.中成药中非法添加降压类化学成分的薄层检测方法的研究[J].中国卫生检验杂志,2009,19(3):530-531+536.
[5]胡青,张甦,王柯,等.中药及保健食品中违禁添加7种降压类化学药物的HPLC-DAD法测定[J].中国医药工业杂志,2010,4(8):601-3.
[6]吴毅,刘绪平,王栋,等.中药制剂及保健品中添加的10种抗高血压药物监测[J].中国药事,2014,28(5):495-500.
[7]陈林,温家欣,齐春艳,等.降压类中成药及保健食品中21种非法添加化学药物的快速检测与确证方法研究[J].分析测试学报,2016,35(8):937-42.
[8]王琤帅,舒展,朱洁,等.中成药和保健食品中18种非法添加降压药物的HPLC-QTOF/MS定性检测[J].中国药师,2016,19(6):1084-7.
[9]李涛,朱小红,林芳.GC-MS联用方法检测降压类中成药及保健食品中非法添加的化学药物[J].药物分析杂志,2010,30(11):2212-5.
[10]国家食品药品监督管理局药品检验补充检验方法和检验项目批准件[S]:2009032.
[2]郭宝平.保健食品中非法添加药物的检测方法的研究[J].天津药学,2013,25(6):62-4.
[3]吴公平,雷玉萍,黎银波,等.辅助降压类保健品中非法添加化学药品的快速筛选方法[J].中国药业,2010,19(14):42-3.
[4]郑荣,王柯,季申.中成药中非法添加降压类化学成分的薄层检测方法的研究[J].中国卫生检验杂志,2009,19(3):530-531+536.
[5]胡青,张甦,王柯,等.中药及保健食品中违禁添加7种降压类化学药物的HPLC-DAD法测定[J].中国医药工业杂志,2010,4(8):601-3.
[6]吴毅,刘绪平,王栋,等.中药制剂及保健品中添加的10种抗高血压药物监测[J].中国药事,2014,28(5):495-500.
[7]陈林,温家欣,齐春艳,等.降压类中成药及保健食品中21种非法添加化学药物的快速检测与确证方法研究[J].分析测试学报,2016,35(8):937-42.
[8]王琤帅,舒展,朱洁,等.中成药和保健食品中18种非法添加降压药物的HPLC-QTOF/MS定性检测[J].中国药师,2016,19(6):1084-7.
[9]李涛,朱小红,林芳.GC-MS联用方法检测降压类中成药及保健食品中非法添加的化学药物[J].药物分析杂志,2010,30(11):2212-5.
[10]国家食品药品监督管理局药品检验补充检验方法和检验项目批准件[S]:2009032.