摘要
目的建立同时测定肿痛安胶囊中:天麻素、升麻素苷、5-O-甲基维斯阿米醇苷、欧前胡素和异欧前胡素含量的HPLC法。方法色谱柱:Phenomenex Luna C_(18)柱(250mm×4.6mm,5μm);流速:1.0mL·min~(-1);柱温:25℃;流动相:甲醇-水梯度洗脱;检测波长:230nm;进样量:5μL。结果天麻素质量浓度在12.46~199.30μg·mL~(-1)范围内线性关系良好,r_1=0.999 5,平均回收率为101.2%,RSD值为1.6%(n=6);升麻素苷质量浓度在12.07~193.20μg·mL~(-1)范围内线性关系良好,r_2=0.999 7,平均回收率为100.1%,RSD值为1.8%(n=6);5-O-甲基维斯阿米醇苷质量浓度在8.12~129.90μg·mL~(-1)范围内线性关系良好,r_3=0.999 8,平均回收率为99.1%,RSD值为1.7%(n=6);欧前胡素质量浓度在12.52~200.40μg·mL~(-1)范围内线性关系良好,r_4=0.999 6,平均回收率为98.6%,RSD值为1.4%(n=6);异欧前胡素质量浓度在12.65~202.40μg·mL~(-1)范围内线性关系良好,r_5=0.999 6,平均回收率为99.0%,RSD值为0.7%(n=6)。结论该方法简单、准确,可同时测定5种成分的含量,可用于肿痛安胶囊的质量控制。
Objective To establish an HPLC method for the determination of gastrodin,prim-O-glucosylcimifugin,5-O-methylvisammioside,imperatorin and isoimperatorin in Zhongtongan Capsules.Methods A Phenomenex Luna C_(18)column(250mm×4.6mm,5μm)was used and the column temperature was 25℃.The flow rate was 1.0mL·min~(-1)and the mobile phase consisted of methanol-water with gradient elution.The detection wavelength was 230nm and the injection volume was 5μL.Results The linear range of gastrodin was 12.46-199.30μg·mL~(-1)(r_1=0.999 5),and the average recovery was 101.2%(RSD=1.6%,n=6);the linear range of prim-O-glucosylcimifugin was 12.07-193.20μg·mL~(-1)(r_2=0.999 7),and the average recovery was 100.1%(RSD=1.8%,n=6);the linear range of 5-O-methylvisammioside was 8.12-129.90μg·mL~(-1)(r_3=0.999 8)and the average recovery was 99.1%(RSD=1.7%,n=6);the linear range of imperatorin was 12.52-200.40μg·mL~(-1)(r_4=0.999 6)and the average recovery was 98.6%(RSD=1.4%,n=6);the linear range of isoimperatorin was 12.65-202.40μg·mL~(-1)(r_5=0.999 6)and the average recovery was 99.0%(RSD=0.7%,n=6).Conclusion The method is simple,accurate and the content of 5ingredients can be determined simultaneously.The method can be used in the quality control of Zhongtongan Capsules.
引文
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