木蝴蝶对SHR大鼠的降血压作用研究
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摘要
目的探讨木蝴蝶对SHR大鼠的降血压作用。方法选取SHR大鼠20只,按照随机数字表法分为模型组、苯那普利组、木蝴蝶高剂量组、木蝴蝶低剂量组,每组各5只,另选取5只雄性Wistar大鼠作为空白对照组,模型组给予2 ml/d生理盐水,苯那普利组给予10 mg/(kg·d)苯那普利,木蝴蝶高剂量组给予2 ml/d木蝴蝶提取液,木蝴蝶低剂量组给予0.5 ml/d木蝴蝶提取液,空白对照组给予2 ml/d生理盐水,均灌胃给药,每天1次,连续给药2个月。于治疗前后分别测大鼠动脉收缩压(SBP)和血清中超氧化物歧化酶(SOD)含量。结果苯那普利组和木蝴蝶低剂量组给药30 d后的SBP低于给药前,差异有统计学意义(P<0.05),而空白对照组、模型组和木蝴蝶高剂量组与给药前比较,差异无统计学意义(P>0.05);空白对照组、木蝴蝶低剂量组给药30 d后的SBP低于模型组,差异有统计学意义(P<0.05)。木蝴蝶低剂量组、木蝴蝶高剂量组给药60 d后的SBP低于给药前,差异有统计学意义(P<0.05),而空白对照组、模型组和苯那普利组与给药前比较,差异无统计学意义(P>0.05);空白对照组、木蝴蝶低剂量组给药60 d后的SBP低于模型组,差异有统计学意义(P<0.05)。苯那普利组、木蝴蝶高剂量组给药60 d后的血清SOD含量高于给药前,差异有统计学意义(P<0.05),而空白对照组、模型组和木蝴蝶低剂量组与给药前比较,差异无统计学意义(P>0.05);苯那普利组给药60 d后的血清SOD含量高于模型组,差异有统计学意义(P<0.05);苯那普利组、木蝴蝶高剂量组给药60 d后的血清SOD含量高于模型组,差异有统计学意义(P<0.05)。结论木蝴蝶对SHR大鼠具有降血压作用,效果明显。
Objective To discuss the hypotensive effect of Oroxylum indicum(L.) Vent on spontaneously hypertensive rats(SHR) rats. Methods All of 20 SHR rats were selected and they were divided into model group, Benazepril group,high-dosage group of Oroxylum indicum(L.) Vent group and low-dosage group of Oroxylum indicum(L.) Vent, 5 cases in each group. 5 male Wistar rats were selected as blank control group. Model group was given 2 ml/d of normal saline,Benazepril group was given 10 mg/(kg·d) of Benazepril, high-dosage group of Oroxylum indicum(L.) Vent group was given 2 ml/d of Oroxylum indicum(L.) Vent extraction solution, low-dosage group of Oroxylum indicum(L.) Vent was given 0.5 ml/d of Oroxylum indicum(L.) Vent, blank control group was given 2 ml/d of normal saline, and they were treated with intragastric administration, once a day for 2 months. The arterial systolic blood pressure(SBP) and superoxide dismutase(SOD) in serum of rats were measured before and after treatment. Results The SBP of Benazepril group and low-dosage group of Oroxylum indicum(L.) Vent after 30 days administration were lower than those before administration, and the differences were statistically significant(P<0.05), but there were no significant differences in blank control group, model group and high dose group compared with before administration(P>0.05). SBP of blank control group and low-dosage group of Oroxylum indicum(L.) Vent after 30 days administration after 30 days administration were lower than those of model group, and the differences were statistically significant(P<0.05). SBP in lowdosage group of Oroxylum indicum(L.) Vent and highdosage group of Oroxylum indicum(L.) Vent after 60 days administration were lower than those before administration,and the differences were statistically significant(P<0.05), but there was no significant differences between blank control group, model group and Benazepril group compared with those before administration(P>0.05). SBP of blank control group and low-dosage group of Oroxylum indicum(L.) Vent after 60 days administration were lower than those of model group, and the differences were statistically significant(P<0.05). The serum SOD content in Benazepril group and high-dosage group of Oroxylum indicum(L.) Vent were higher than those before administration, and the differences were statistically significant(P <0.05), but there were no significant differences between blank control group,model group and low-dosage group of Oroxylum indicum(L.) Vent compoared with before administration(P>0.05). The serum SOD content in Benazepril group after 60 days administration were higher than that in model group, and the difference was statistically significant(P<0.05). The serum SOD content in Benazepril group and high-dosage group of Oroxylum indicum(L.) Vent after 60 days administration were higher than those in model group, and the differences were statistically significant(P<0.05). Conclusion Oroxylum indicum(L.) Vent has antihypertensive effect on SHR rats,and the effect is obvious.
Objective To discuss the hypotensive effect of Oroxylum indicum(L.) Vent on spontaneously hypertensive rats(SHR) rats. Methods All of 20 SHR rats were selected and they were divided into model group, Benazepril group,high-dosage group of Oroxylum indicum(L.) Vent group and low-dosage group of Oroxylum indicum(L.) Vent, 5 cases in each group. 5 male Wistar rats were selected as blank control group. Model group was given 2 ml/d of normal saline,Benazepril group was given 10 mg/(kg·d) of Benazepril, high-dosage group of Oroxylum indicum(L.) Vent group was given 2 ml/d of Oroxylum indicum(L.) Vent extraction solution, low-dosage group of Oroxylum indicum(L.) Vent was given 0.5 ml/d of Oroxylum indicum(L.) Vent, blank control group was given 2 ml/d of normal saline, and they were treated with intragastric administration, once a day for 2 months. The arterial systolic blood pressure(SBP) and superoxide dismutase(SOD) in serum of rats were measured before and after treatment. Results The SBP of Benazepril group and low-dosage group of Oroxylum indicum(L.) Vent after 30 days administration were lower than those before administration, and the differences were statistically significant(P<0.05), but there were no significant differences in blank control group, model group and high dose group compared with before administration(P>0.05). SBP of blank control group and low-dosage group of Oroxylum indicum(L.) Vent after 30 days administration after 30 days administration were lower than those of model group, and the differences were statistically significant(P<0.05). SBP in lowdosage group of Oroxylum indicum(L.) Vent and highdosage group of Oroxylum indicum(L.) Vent after 60 days administration were lower than those before administration,and the differences were statistically significant(P<0.05), but there was no significant differences between blank control group, model group and Benazepril group compared with those before administration(P>0.05). SBP of blank control group and low-dosage group of Oroxylum indicum(L.) Vent after 60 days administration were lower than those of model group, and the differences were statistically significant(P<0.05). The serum SOD content in Benazepril group and high-dosage group of Oroxylum indicum(L.) Vent were higher than those before administration, and the differences were statistically significant(P <0.05), but there were no significant differences between blank control group,model group and low-dosage group of Oroxylum indicum(L.) Vent compoared with before administration(P>0.05). The serum SOD content in Benazepril group after 60 days administration were higher than that in model group, and the difference was statistically significant(P<0.05). The serum SOD content in Benazepril group and high-dosage group of Oroxylum indicum(L.) Vent after 60 days administration were higher than those in model group, and the differences were statistically significant(P<0.05). Conclusion Oroxylum indicum(L.) Vent has antihypertensive effect on SHR rats,and the effect is obvious.
引文
[1]崔翠新,陆高翔.百色市周边地区壮族人群高血压流行特点研究[J].中西医结合心血管病杂志,2013,11(5):589-590.
[2]张昌壮,张培旭,姚华,等.HPLC法测定木蝴蝶中木蝴蝶苷A和木蝴蝶苷B的质量分数[J].吉林大学学报(理学版),2013,51(2):321-324.
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[5]李朵,彭耀金,陈阿,等.白杨素对TNF-α诱导慢性暴露TGF-β卵巢癌OVCAR-3细胞系球形成的影响[J].中国药理学通报,2016,32(8):1133-1137.
[6]蒋嘉烨,王现珍,栗源,等.自发性高血压大鼠高血压进程中心肌血管紧张素转换酶的变化及卡托普利干预作用[J].中国药理学与毒理学杂志,2013,27(6):954-958.
[7]王晶,林晓杰,罗晓舟,等.针刺太溪穴降低SHR血压及调节延髓TNF-α、IL-6蛋白表达[J].暨南大学学报(自然科学与医学版),2016,37(4):327-330.
[8]杨竞霄,白宝宝,王海燕,等.马来酸依那普利对高血压大鼠心肌细胞内质网应激的影响及与左室肥厚的关系[J].山西医科大学学报,2015,46(8):729-733.
[9]顾磊科,沈凯.过氧化氢酶在血管紧张素Ⅱ诱导的高血压大鼠血管外膜中表达及与血管壁重塑相关性[J].现代实用医学,2017,29(8):999-1001,封2.
[10]张倩,丁菁,杨飞,等.依普利酮对高盐诱导的高血压大鼠主动脉内皮型一氧化氮合酶表达及活性的影响[J].中国病理生理杂志,2015,31(9):1606-1610.
[11]邱方,熊开宇,石丽君,等.褪黑素在有氧运动改善自发性高血压大鼠血压和肠系膜动脉功能中的作用[J].中国动脉硬化杂志,2015,23(12):1229-1235.
[12]刘炜,朱陵群,陈思思,等.针刺捻转补泻手法对应激性血压升高大鼠血浆乙酰胆碱及胸主动脉一氧化氮合酶、环鸟苷酸含量的影响[J].针刺研究,2015,40(2):136-140.
[13]张金艳,赵乐,李贻奎,等.丹皮酚对自发性高血压大鼠动脉血压和血流量的影响及其与血管舒缩相关的作用机制[J].中国中药杂志,2015,40(24):4903-4907.
[14]伊琳,陈蓓蓓,孙少伯,等.当归挥发油对自发性高血压大鼠心肌组织中微小RNA差异表达谱的影响[J].吉林大学学报(医学版),2016,42(4):671-675.
[15]李婕,严士海,王欣彤,等.基于高盐诱导的SHR大鼠肾损伤探讨中医理论“盐胜血”的机理研究[J].南京中医药大学学报,2016,32(3):287-290.
[16]徐召霞,赵腾腾,柏平,等.不同强度有氧运动对SHR动脉平滑肌MaxiK通道功能重构的影响[J].天津体育学院学报,2016,31(6):479-484.
[17]金华,刘志军,颜春鲁,等.贝那普利和氨氯地平对自发性高血压大鼠促胰液素、生长抑素表达的影响[J].中国应用生理学杂志,2018,34(2):154-158,后插1.
[18]许东辉,李东原,黄淑华,等.WKY大鼠与自发性高血压模型大鼠(SHR)大脑中动脉拉伸力学特性的对比分析[J].生物医学工程研究,2017,36(1):86-89.
[19]夏磊,胡志和,李艳军,等.含血管紧张素转化酶抑制肽的酪蛋白水解物与紫薯提取物联用对原发性高血压大鼠血压的影响[J].食品科学,2016,37(19):243-248.
[2]张昌壮,张培旭,姚华,等.HPLC法测定木蝴蝶中木蝴蝶苷A和木蝴蝶苷B的质量分数[J].吉林大学学报(理学版),2013,51(2):321-324.
[3]刘媛,龙梅,谢孟峡,等.白杨素与不同构型人血清白蛋白的作用机制[J].物理化学学报,2013,29(12):2647-2654.
[4]李华珍,陈艳红,方银兰,等.白杨素对SKOV3细胞系干样细胞球形成和CK2α表达的影响[J].中华医学杂志,2016,96(25):2013-2016.
[5]李朵,彭耀金,陈阿,等.白杨素对TNF-α诱导慢性暴露TGF-β卵巢癌OVCAR-3细胞系球形成的影响[J].中国药理学通报,2016,32(8):1133-1137.
[6]蒋嘉烨,王现珍,栗源,等.自发性高血压大鼠高血压进程中心肌血管紧张素转换酶的变化及卡托普利干预作用[J].中国药理学与毒理学杂志,2013,27(6):954-958.
[7]王晶,林晓杰,罗晓舟,等.针刺太溪穴降低SHR血压及调节延髓TNF-α、IL-6蛋白表达[J].暨南大学学报(自然科学与医学版),2016,37(4):327-330.
[8]杨竞霄,白宝宝,王海燕,等.马来酸依那普利对高血压大鼠心肌细胞内质网应激的影响及与左室肥厚的关系[J].山西医科大学学报,2015,46(8):729-733.
[9]顾磊科,沈凯.过氧化氢酶在血管紧张素Ⅱ诱导的高血压大鼠血管外膜中表达及与血管壁重塑相关性[J].现代实用医学,2017,29(8):999-1001,封2.
[10]张倩,丁菁,杨飞,等.依普利酮对高盐诱导的高血压大鼠主动脉内皮型一氧化氮合酶表达及活性的影响[J].中国病理生理杂志,2015,31(9):1606-1610.
[11]邱方,熊开宇,石丽君,等.褪黑素在有氧运动改善自发性高血压大鼠血压和肠系膜动脉功能中的作用[J].中国动脉硬化杂志,2015,23(12):1229-1235.
[12]刘炜,朱陵群,陈思思,等.针刺捻转补泻手法对应激性血压升高大鼠血浆乙酰胆碱及胸主动脉一氧化氮合酶、环鸟苷酸含量的影响[J].针刺研究,2015,40(2):136-140.
[13]张金艳,赵乐,李贻奎,等.丹皮酚对自发性高血压大鼠动脉血压和血流量的影响及其与血管舒缩相关的作用机制[J].中国中药杂志,2015,40(24):4903-4907.
[14]伊琳,陈蓓蓓,孙少伯,等.当归挥发油对自发性高血压大鼠心肌组织中微小RNA差异表达谱的影响[J].吉林大学学报(医学版),2016,42(4):671-675.
[15]李婕,严士海,王欣彤,等.基于高盐诱导的SHR大鼠肾损伤探讨中医理论“盐胜血”的机理研究[J].南京中医药大学学报,2016,32(3):287-290.
[16]徐召霞,赵腾腾,柏平,等.不同强度有氧运动对SHR动脉平滑肌MaxiK通道功能重构的影响[J].天津体育学院学报,2016,31(6):479-484.
[17]金华,刘志军,颜春鲁,等.贝那普利和氨氯地平对自发性高血压大鼠促胰液素、生长抑素表达的影响[J].中国应用生理学杂志,2018,34(2):154-158,后插1.
[18]许东辉,李东原,黄淑华,等.WKY大鼠与自发性高血压模型大鼠(SHR)大脑中动脉拉伸力学特性的对比分析[J].生物医学工程研究,2017,36(1):86-89.
[19]夏磊,胡志和,李艳军,等.含血管紧张素转化酶抑制肽的酪蛋白水解物与紫薯提取物联用对原发性高血压大鼠血压的影响[J].食品科学,2016,37(19):243-248.