Preliminary Study on Biological Control of Mycotoxin Contamination in Agricultural Products and Its Mechanism
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摘要
Aflatoxin is the most powerful carcinogen and highly toxic mycotoxin known in nature. It can cause serious toxin pollution to agricultural products, foods and fodders, and is extremely harmful to human and animal health. For aflatoxin pollution, the emphasis is on the control of source, and biological control is favored by scientists because of its advantages of environmental protection and ecological safety. According to the morphology, color and appearance of microbial colonies isolated from maize kernels, 300 bacterial strains were obtained from main maize-producing areas including Tangshan City, Handan City, Shijiazhuang City and Cangzhou City. Two biocontrol bacterial strains(B120 and B65) having a strong control effect on aflatoxins were screened. After identification by molecular biology and physical and chemical properties, the two biocontrol bacterial strains were identified as Bacillus amyloliquefaciens. Their effects on spore germination, mycelial growth and toxin degradation of Aspergillus flavus were investigated. The results showed that strains B120 and B65 could significantly inhibit the spore germination and mycelial growth of A. flavus and reduce aflatoxin production. This study provides a new way to prevent and control the pollution control of aflatoxins in agricultural products.
Aflatoxin is the most powerful carcinogen and highly toxic mycotoxin known in nature. It can cause serious toxin pollution to agricultural products, foods and fodders, and is extremely harmful to human and animal health. For aflatoxin pollution, the emphasis is on the control of source, and biological control is favored by scientists because of its advantages of environmental protection and ecological safety. According to the morphology, color and appearance of microbial colonies isolated from maize kernels, 300 bacterial strains were obtained from main maize-producing areas including Tangshan City, Handan City, Shijiazhuang City and Cangzhou City. Two biocontrol bacterial strains(B120 and B65) having a strong control effect on aflatoxins were screened. After identification by molecular biology and physical and chemical properties, the two biocontrol bacterial strains were identified as Bacillus amyloliquefaciens. Their effects on spore germination, mycelial growth and toxin degradation of Aspergillus flavus were investigated. The results showed that strains B120 and B65 could significantly inhibit the spore germination and mycelial growth of A. flavus and reduce aflatoxin production. This study provides a new way to prevent and control the pollution control of aflatoxins in agricultural products.
Aflatoxin is the most powerful carcinogen and highly toxic mycotoxin known in nature. It can cause serious toxin pollution to agricultural products, foods and fodders, and is extremely harmful to human and animal health. For aflatoxin pollution, the emphasis is on the control of source, and biological control is favored by scientists because of its advantages of environmental protection and ecological safety. According to the morphology, color and appearance of microbial colonies isolated from maize kernels, 300 bacterial strains were obtained from main maize-producing areas including Tangshan City, Handan City, Shijiazhuang City and Cangzhou City. Two biocontrol bacterial strains(B120 and B65) having a strong control effect on aflatoxins were screened. After identification by molecular biology and physical and chemical properties, the two biocontrol bacterial strains were identified as Bacillus amyloliquefaciens. Their effects on spore germination, mycelial growth and toxin degradation of Aspergillus flavus were investigated. The results showed that strains B120 and B65 could significantly inhibit the spore germination and mycelial growth of A. flavus and reduce aflatoxin production. This study provides a new way to prevent and control the pollution control of aflatoxins in agricultural products.
引文
[1]FAN SF,LI PW,WANG XP,et al. Comparison on determination of aflatoxins in peanut,corn and rice by liquid chromatography and liquid chromatography-tandem mass spectrometry[J]. Food Science,2011,32(12):254-258.(in Chinese)
[2]LIU X. Strengthen research work of exposure and control of mycotoxin[J]. Chinese Journal of Preventive Medicine,2006,40:307-308.
[3]ALPERT ME,HUTC MSR,WBGAN GN. Association between aflatoxin content of food and hepatoma frequency in Uganda[J]. Cancer,1971,(28):253-260.
[4]JACKOBSON WC,WISEMAN HG. The transmission of aftatoxin B1 into eggs[J]. Poultry Science,1974,53:1743-1745.
[5]TRUCKESS MW,STOLOFF L,YONG K. Aflatoxins B1 and M1 in eggs and tissues of laying hens consuming aftatoxin contaminated feed[J].Poultry Science,1983,62:2176-2182.
[6]IBEH IN,URAIH N,OGONAR JI. Dietary exposure to aflatoxin in human male infertility in Benin City,Nigeria[J]. International iournal of fertility and menopause studies,1994,4:208-214.
[7]DEY R,PAL KK,BHATT DM,et al. Growth promotion and yieid enhancement of peanut(Arachis hypogaea L.)by application of plant growth-promoting rhizobacteria[J]. Microbiological Research,2004,159(4):371-394.
[8]HE H,QIU EX,HU FP,et al. Advance in biological effects of endophytic bacteria[J]. Journal of Microbiology,2004,24(3):40-45.(in Chinese)
[9]YAO YP,ZHANG LT,ZHENG BQ,et al. Screening of peanut endophytic bacterial antagonists against Aspergillus flavus,and identification and antagonistic activities of strain B85-1[J]. Journal of Peanut Science,2016,45(3):20-26.(in Chinese)
[10]YAO YP,DING D,ZHANG LT,XIANG AL,et al. Screening of biocontrol bacteria against Aspergillus flavus and antifungal activity of strain B42-3[J]. Journal of the Chinese Cereals and Oils Association,2018,33(3):84-88.
[11]HUANG Y,ADAMS MC. In vitro assessment of the upper gastrointestinal tolerance of potential probiotic dairy propionibacteria[J]. International Journal of Food Microbiology,2004,91(3):253-260.
[12]DONG XZ,CAI MY. Manual of identification in common bacteria[M].Beijing:Science Press,2001.(in Chinese)
[13]KIRK GD,BAH E,MONTESANO R. Molecular epidemiology of human liver cancer:Insights into etiology,pathogenesis and prevention from The Gambia[J]. Carcinogenesis,2006,27:2070-82.
[14]GROOPMAN JD,KENSLER TW,WILD CP. Protective interventions to prevent aflatoxin-induced carcinogenesis in developing countries[J].Annual Review of Public Health,2008,29:187-203.
[15]WU F,KHLANGWISET P. Health economic impacts and cost-effectiveness of aflatoxin reduction strategies in Africa:Case studies in biocontrol and postharvest interventions[J]. Food Additives and Contaminants,2010,27:496-509.
[16]LIU Y,WU F. Global burden of aflatoxin-induced hepatocellular carcinoma:A risk assessment[J]. Environmental Health Perspectives,2010,118:18-24.
[17]VAN EGMOND,DRAGACCI S. Liquid chromatographic method for aflatoxin M1 in milk[J]. Methods in Molecular Biology,2001,157:59-69.
[18]TEKINSEN K,EKEN HS. Aflatoxin M1 levels in UHT milk and kashar cheese consumed in Turkey[J]. Food and Chemical Toxicology,2008,46:3287-3289.
[19]KONG Q,LIU QZ,YU FT,et al. Inhibitory effect on the growth and aflatoxin biosynthesis of Aspergillus flavus by a strain of marine Bacillus[J]. Journal of Zhejiang University(Agriculture&Life Sciences),2010,36(4):387-392.(in Chinese)
[20]ABBAS HK,ZABLOTOWICZ RM,BRUNS HA. Biocontrol of aflatoxin in corn by inoculation with non-aflatoxigenic Aspergillus flavus isolate[J]. Biocontrol Science and Technology,2006,16(5):437-449.
[2]LIU X. Strengthen research work of exposure and control of mycotoxin[J]. Chinese Journal of Preventive Medicine,2006,40:307-308.
[3]ALPERT ME,HUTC MSR,WBGAN GN. Association between aflatoxin content of food and hepatoma frequency in Uganda[J]. Cancer,1971,(28):253-260.
[4]JACKOBSON WC,WISEMAN HG. The transmission of aftatoxin B1 into eggs[J]. Poultry Science,1974,53:1743-1745.
[5]TRUCKESS MW,STOLOFF L,YONG K. Aflatoxins B1 and M1 in eggs and tissues of laying hens consuming aftatoxin contaminated feed[J].Poultry Science,1983,62:2176-2182.
[6]IBEH IN,URAIH N,OGONAR JI. Dietary exposure to aflatoxin in human male infertility in Benin City,Nigeria[J]. International iournal of fertility and menopause studies,1994,4:208-214.
[7]DEY R,PAL KK,BHATT DM,et al. Growth promotion and yieid enhancement of peanut(Arachis hypogaea L.)by application of plant growth-promoting rhizobacteria[J]. Microbiological Research,2004,159(4):371-394.
[8]HE H,QIU EX,HU FP,et al. Advance in biological effects of endophytic bacteria[J]. Journal of Microbiology,2004,24(3):40-45.(in Chinese)
[9]YAO YP,ZHANG LT,ZHENG BQ,et al. Screening of peanut endophytic bacterial antagonists against Aspergillus flavus,and identification and antagonistic activities of strain B85-1[J]. Journal of Peanut Science,2016,45(3):20-26.(in Chinese)
[10]YAO YP,DING D,ZHANG LT,XIANG AL,et al. Screening of biocontrol bacteria against Aspergillus flavus and antifungal activity of strain B42-3[J]. Journal of the Chinese Cereals and Oils Association,2018,33(3):84-88.
[11]HUANG Y,ADAMS MC. In vitro assessment of the upper gastrointestinal tolerance of potential probiotic dairy propionibacteria[J]. International Journal of Food Microbiology,2004,91(3):253-260.
[12]DONG XZ,CAI MY. Manual of identification in common bacteria[M].Beijing:Science Press,2001.(in Chinese)
[13]KIRK GD,BAH E,MONTESANO R. Molecular epidemiology of human liver cancer:Insights into etiology,pathogenesis and prevention from The Gambia[J]. Carcinogenesis,2006,27:2070-82.
[14]GROOPMAN JD,KENSLER TW,WILD CP. Protective interventions to prevent aflatoxin-induced carcinogenesis in developing countries[J].Annual Review of Public Health,2008,29:187-203.
[15]WU F,KHLANGWISET P. Health economic impacts and cost-effectiveness of aflatoxin reduction strategies in Africa:Case studies in biocontrol and postharvest interventions[J]. Food Additives and Contaminants,2010,27:496-509.
[16]LIU Y,WU F. Global burden of aflatoxin-induced hepatocellular carcinoma:A risk assessment[J]. Environmental Health Perspectives,2010,118:18-24.
[17]VAN EGMOND,DRAGACCI S. Liquid chromatographic method for aflatoxin M1 in milk[J]. Methods in Molecular Biology,2001,157:59-69.
[18]TEKINSEN K,EKEN HS. Aflatoxin M1 levels in UHT milk and kashar cheese consumed in Turkey[J]. Food and Chemical Toxicology,2008,46:3287-3289.
[19]KONG Q,LIU QZ,YU FT,et al. Inhibitory effect on the growth and aflatoxin biosynthesis of Aspergillus flavus by a strain of marine Bacillus[J]. Journal of Zhejiang University(Agriculture&Life Sciences),2010,36(4):387-392.(in Chinese)
[20]ABBAS HK,ZABLOTOWICZ RM,BRUNS HA. Biocontrol of aflatoxin in corn by inoculation with non-aflatoxigenic Aspergillus flavus isolate[J]. Biocontrol Science and Technology,2006,16(5):437-449.