葵花盘粉有效成分对小鼠高尿酸血症的治疗作用
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摘要
目的:探讨葵花盘粉主要成分小分子肽、生物碱、总黄酮和多糖等对小鼠高尿酸血症的治疗作用,阐明葵花盘粉对小鼠血尿酸(UA)水平和大鼠关节肿胀的影响及对肝和肾组织的保护作用。方法:取昆明雄鼠96只,随机分为8组,各组12只,一组为空白对照组,其余用作高尿酸血症模型制备。高尿酸血症模型造模成功的小鼠随机分为模型组、阳性对照(别嘌醇)组、小分子肽组、生物碱组、总黄酮组、多糖组和葵花盘粉组,分别灌胃给药,给药7d后检测各组小鼠血UA水平;取每组小鼠肝和肾组织,采用HE染色法观察各组小鼠肝和肾组织形态表现。在雄性SD大鼠膝关节腔注射3mg尿酸钠,建立大鼠急性痛风性关节炎模型。实验分为空白组、模型组、阳性对照药秋水仙碱组、不同浓度(0%、20%、40%、60%、80%和100%)乙醇葵花盘提取物(SDE)组,每组4只。分别测定0、12、24和48h大鼠关节周长。采用ELISA试剂盒检测各组大鼠血清中相关炎症因子白细胞介素10(IL-10)和巨噬细胞炎性蛋白1α(MIP-1α)水平。结果:与模型组比较,别嘌醇组、小分子肽组、生物碱组、总黄酮组和葵花盘粉组小鼠血UA水平均在给药后明显降低(P<0.05),其中别嘌醇组小鼠血UA水平降低最大,小分子肽组次之。HE染色观察,小分子肽组小鼠肝小叶内肝细胞及肝细胞索清晰,肝细胞胞质饱满,且肾细胞与空白对照组比较未见异常。注射尿酸钠48h后,与模型组比较,SDE组大鼠关节肿胀度最多减少27.1%。与模型组比较,20%SDE组大鼠血清IL-10水平明显升高(P<0.01),血清中MIP-1α水平亦明显升高(P<0.01)。结论:葵花盘粉可降低血UA水平,并对肝和肾组织有一定的保护作用,且可通过提高机体抗炎活性降低机体炎症反应,实现抗痛风性关节炎活性的作用。
Objective:To investigate the therapeutic effects of small peptide,alkaloid,total flavonoids and polysaccharides extracted from sunflower plate powder on the hyperuricemia in the mice,and to clarify that the influence of sunflower powder in the uric acid(UA)level and joint swelling and its protective effect on the liver and kidney tissues.Methods:A total of 96 male Kunming rats were randomly divided into 8 groups,each group of12 rats.One group was used as blank control group,the others were used to set up hyperuricemia models.The successful mouse models of hyperuricemia were randomly divided into model group and positive control group(allopurinol group),small molecule peptide group,alkaloi group,total flavone group,polysaccharide group and sunflower disk group.The serum UA levels were measured 7 dafter administration and the liver and kidney tissues of the mice in various groups were taken.The morphological changes of liver and kidney tissues were observed by HE staining.Acute gouty arthritis model was established by injecting 3 mg sodium urate into the knee joint of the male SD rats.The experiment was divided into blank group,model group,positive control colchicine group,sunflower disc extracts(SDE)group with different concentrations(0%,20%,40%,60%,80% and 100%),4 rats in each group.The joint circumference was measured at 0,12,24 and 48 h,respectively.The serum levels of interleukin-10(IL-10)and macrophage inflammatory protein-1α(MIP-1α) were detected by ELISA kit.Results:Compared with model group,the serum UA levels of the mice in allopurinol group,small molecule peptide group,alkaloid group,total flavonoids group and sunflower group were significantly decreased after administration(P<0.05);and the serum UA level in allopurinol group was decreased the most,followed by small molecule peptide group.The HE staining results showed that the hepatocytes in small molecule peptide group were clear,the hepatocytes had full cytoplasm,and no abnormality was observed in the kidney cells compared with blank control group.After 48 hinjection of sodium urate,the degree of joint swelling was reduced by up to 27.1%in SDE group compared with model group.Compared with model group,the serum IL-10 level in 20% SDE group was significantly increased(P <0.01),and the serum MIP-1αlevel was also significantly increased(P <0.01).Conclusion:Sunflower powder can reduce the UA level and has protective effect on the liver and kidney tissues and it can improve the body's anti-inflammatory activity and reduce the body's inflammatory response to achieve anti-gout arthritis activity.
Objective:To investigate the therapeutic effects of small peptide,alkaloid,total flavonoids and polysaccharides extracted from sunflower plate powder on the hyperuricemia in the mice,and to clarify that the influence of sunflower powder in the uric acid(UA)level and joint swelling and its protective effect on the liver and kidney tissues.Methods:A total of 96 male Kunming rats were randomly divided into 8 groups,each group of12 rats.One group was used as blank control group,the others were used to set up hyperuricemia models.The successful mouse models of hyperuricemia were randomly divided into model group and positive control group(allopurinol group),small molecule peptide group,alkaloi group,total flavone group,polysaccharide group and sunflower disk group.The serum UA levels were measured 7 dafter administration and the liver and kidney tissues of the mice in various groups were taken.The morphological changes of liver and kidney tissues were observed by HE staining.Acute gouty arthritis model was established by injecting 3 mg sodium urate into the knee joint of the male SD rats.The experiment was divided into blank group,model group,positive control colchicine group,sunflower disc extracts(SDE)group with different concentrations(0%,20%,40%,60%,80% and 100%),4 rats in each group.The joint circumference was measured at 0,12,24 and 48 h,respectively.The serum levels of interleukin-10(IL-10)and macrophage inflammatory protein-1α(MIP-1α) were detected by ELISA kit.Results:Compared with model group,the serum UA levels of the mice in allopurinol group,small molecule peptide group,alkaloid group,total flavonoids group and sunflower group were significantly decreased after administration(P<0.05);and the serum UA level in allopurinol group was decreased the most,followed by small molecule peptide group.The HE staining results showed that the hepatocytes in small molecule peptide group were clear,the hepatocytes had full cytoplasm,and no abnormality was observed in the kidney cells compared with blank control group.After 48 hinjection of sodium urate,the degree of joint swelling was reduced by up to 27.1%in SDE group compared with model group.Compared with model group,the serum IL-10 level in 20% SDE group was significantly increased(P <0.01),and the serum MIP-1αlevel was also significantly increased(P <0.01).Conclusion:Sunflower powder can reduce the UA level and has protective effect on the liver and kidney tissues and it can improve the body's anti-inflammatory activity and reduce the body's inflammatory response to achieve anti-gout arthritis activity.
引文
[1]刘小波,窦愈常,薛均来.葵花盘粉颗粒抗痛风作用机理及有效部位分析[J].医学信息,2016,29(33):316.
[2]刘小波,薛均来.一种抗痛风中草药葵花盘粉的药理作用[J].内蒙古中医药,2016,35(8):130-131.
[3]薛均来,刘小波.中草药葵花盘粉治疗痛风80例临床观察[J].医药卫生:引文版,2016,4:174.
[4]于舒雁.金钱草总黄酮对高尿酸血症动物模型的影响[D].郑州:河南中医学院,2014.
[5]耿露源,王守富.高尿酸血症中医药治疗进展[J].中医研究,2017,30(2):68-72.
[6]李晓敏,陈泽娜,古洁若.高尿酸血症的现状不容忽视[J].新医学,2016,47(3):137-141.
[7]舒祝明,施政.中医药治疗高尿酸血症临床疗效荟萃分析[J].浙江中西医结合杂志,2017,27(5):439-442.
[8]杨韬,陈真.高尿酸血症的动物模型研究现状和评价[J].安徽医药,2014,18(8):1582-1584.
[9]Bisht M,Bist SS.Febuxostat:a novel agent for management of hyperuricemia in gout[J].Indian J Pharm Sci,2011,73(6):597-600.
[10]李振彬,马旭,杨静,等.白芍总苷治疗大鼠急性痛风性关节炎的作用及对血清IL-1β、IL-6、TNF-α的影响[J].新医学,2017,48(6):380-384.
[11]黄明进,罗春丽,郭刚,等.黑骨藤抗类风湿性关节炎作用及其分子机制[J].中国实验方剂学杂志,2011,17(12):174-177.
[12]文学平,刘德俊,裴忆雪.黄芩苷抗急性痛风性关节炎的实验研究[J].中药材,2017,40(8):1953-1956.
[13]周敏,杨喆,林启展.高尿酸血症的中医药研究进展[J].新中医,2017,49(6):149-151.
[14]温雯,李月红.高尿酸血症肾损害研究进展[J].临床内科杂志,2016,33:309-311.
[15]樊海瑞,赵岩,傅警龙,等.痛风宁胶囊对小鼠高尿酸血症和肾脏功能的改善作用[J].吉林大学学报:医学版,2017,43(5):893-896.
[16]张扬.复方中药组方对高尿酸血症影响的研究[J].中国医院用药评价与分析,2016,16(3):360-363.
[17]刘海娇,范轶鑫,潘秀秀,等.原花青素对小鼠急性痛风性关节炎的实验研究[J].中国现代药物应用,2016,10(22):194-196.
[18]周忠光,丑小月,费洪新,等.丹溪痛风方对小鼠急性痛风性关节炎的实验研究[J].中医药学报,2015,43(1):59-61.
[19]赵晓芳.高尿酸血症与痛风临床分析[J].中国卫生标准管理,2015,6(6):171-172.
[20]张明月,汪悦.高尿酸血症的中医治疗概况[J].中医药导报,2015,21(10):101-104.
[2]刘小波,薛均来.一种抗痛风中草药葵花盘粉的药理作用[J].内蒙古中医药,2016,35(8):130-131.
[3]薛均来,刘小波.中草药葵花盘粉治疗痛风80例临床观察[J].医药卫生:引文版,2016,4:174.
[4]于舒雁.金钱草总黄酮对高尿酸血症动物模型的影响[D].郑州:河南中医学院,2014.
[5]耿露源,王守富.高尿酸血症中医药治疗进展[J].中医研究,2017,30(2):68-72.
[6]李晓敏,陈泽娜,古洁若.高尿酸血症的现状不容忽视[J].新医学,2016,47(3):137-141.
[7]舒祝明,施政.中医药治疗高尿酸血症临床疗效荟萃分析[J].浙江中西医结合杂志,2017,27(5):439-442.
[8]杨韬,陈真.高尿酸血症的动物模型研究现状和评价[J].安徽医药,2014,18(8):1582-1584.
[9]Bisht M,Bist SS.Febuxostat:a novel agent for management of hyperuricemia in gout[J].Indian J Pharm Sci,2011,73(6):597-600.
[10]李振彬,马旭,杨静,等.白芍总苷治疗大鼠急性痛风性关节炎的作用及对血清IL-1β、IL-6、TNF-α的影响[J].新医学,2017,48(6):380-384.
[11]黄明进,罗春丽,郭刚,等.黑骨藤抗类风湿性关节炎作用及其分子机制[J].中国实验方剂学杂志,2011,17(12):174-177.
[12]文学平,刘德俊,裴忆雪.黄芩苷抗急性痛风性关节炎的实验研究[J].中药材,2017,40(8):1953-1956.
[13]周敏,杨喆,林启展.高尿酸血症的中医药研究进展[J].新中医,2017,49(6):149-151.
[14]温雯,李月红.高尿酸血症肾损害研究进展[J].临床内科杂志,2016,33:309-311.
[15]樊海瑞,赵岩,傅警龙,等.痛风宁胶囊对小鼠高尿酸血症和肾脏功能的改善作用[J].吉林大学学报:医学版,2017,43(5):893-896.
[16]张扬.复方中药组方对高尿酸血症影响的研究[J].中国医院用药评价与分析,2016,16(3):360-363.
[17]刘海娇,范轶鑫,潘秀秀,等.原花青素对小鼠急性痛风性关节炎的实验研究[J].中国现代药物应用,2016,10(22):194-196.
[18]周忠光,丑小月,费洪新,等.丹溪痛风方对小鼠急性痛风性关节炎的实验研究[J].中医药学报,2015,43(1):59-61.
[19]赵晓芳.高尿酸血症与痛风临床分析[J].中国卫生标准管理,2015,6(6):171-172.
[20]张明月,汪悦.高尿酸血症的中医治疗概况[J].中医药导报,2015,21(10):101-104.