Effects of the Syzygium aromaticum L. extract on antioxidation and inhibition of matrix metalloproteinase in human dermal fibroblast
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摘要
Objective: To investigate cosmetic potential of Syzygium aromaticum L.(S. aromaticum L.) and to determine its antioxidant and anti-wrinkling effects. Methods: Using highperformance liquid chromatography, eugenol component was quantitated. The antioxidant activity of S. aromaticum L. was analyzed by 2,2-diphenyl-1-picrylhydrazyl radical scavenging and superoxide dismutase like activities. To determine cell viability, elastase and matrix metalloproteinase-1(MMP-1) activity, human dermal fibroblasts(HS68) were treated with S. aromaticum L. The inhibitory effect of S. aromaticum L. on tumor necrosis factor alpha induced MMPs expression in HS68 was analyzed by realtime-PCR. Results: The eugenol content was confirmed in S. aromaticum L. S. aromaticum L. was observed to have high 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and superoxide dismutase like activity. S. aromaticum L. had no cytotoxicity against the HS68 and dose-dependently increased elastase inhibition. Moreover, S. aromaticum L. significantly decreased MMP-1 content and inhibited gene levels of MMP-1, MMP-2, MMP-3 and MMP-9. Conclusions: The findings suggest that S. aromaticum L. has great potential as a cosmeceutical ingredient with antioxidant and antiwrinkling effects.
Objective: To investigate cosmetic potential of Syzygium aromaticum L.(S. aromaticum L.) and to determine its antioxidant and anti-wrinkling effects. Methods: Using highperformance liquid chromatography, eugenol component was quantitated. The antioxidant activity of S. aromaticum L. was analyzed by 2,2-diphenyl-1-picrylhydrazyl radical scavenging and superoxide dismutase like activities. To determine cell viability, elastase and matrix metalloproteinase-1(MMP-1) activity, human dermal fibroblasts(HS68) were treated with S. aromaticum L. The inhibitory effect of S. aromaticum L. on tumor necrosis factor alpha induced MMPs expression in HS68 was analyzed by realtime-PCR. Results: The eugenol content was confirmed in S. aromaticum L. S. aromaticum L. was observed to have high 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and superoxide dismutase like activity. S. aromaticum L. had no cytotoxicity against the HS68 and dose-dependently increased elastase inhibition. Moreover, S. aromaticum L. significantly decreased MMP-1 content and inhibited gene levels of MMP-1, MMP-2, MMP-3 and MMP-9. Conclusions: The findings suggest that S. aromaticum L. has great potential as a cosmeceutical ingredient with antioxidant and antiwrinkling effects.
Objective: To investigate cosmetic potential of Syzygium aromaticum L.(S. aromaticum L.) and to determine its antioxidant and anti-wrinkling effects. Methods: Using highperformance liquid chromatography, eugenol component was quantitated. The antioxidant activity of S. aromaticum L. was analyzed by 2,2-diphenyl-1-picrylhydrazyl radical scavenging and superoxide dismutase like activities. To determine cell viability, elastase and matrix metalloproteinase-1(MMP-1) activity, human dermal fibroblasts(HS68) were treated with S. aromaticum L. The inhibitory effect of S. aromaticum L. on tumor necrosis factor alpha induced MMPs expression in HS68 was analyzed by realtime-PCR. Results: The eugenol content was confirmed in S. aromaticum L. S. aromaticum L. was observed to have high 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and superoxide dismutase like activity. S. aromaticum L. had no cytotoxicity against the HS68 and dose-dependently increased elastase inhibition. Moreover, S. aromaticum L. significantly decreased MMP-1 content and inhibited gene levels of MMP-1, MMP-2, MMP-3 and MMP-9. Conclusions: The findings suggest that S. aromaticum L. has great potential as a cosmeceutical ingredient with antioxidant and antiwrinkling effects.
引文
[1]Park GSY,Lee W,Sung SH,Oh MS.Protection on skin aging mediated by antiapoptosis effects of the water lily(Nymphaea tetragona Georgi)via reactive oxygen species scavenging in human epidermal keratinocytes.Pharmacology 2016;97(5-6):282-293.
[2]Farage MA,Miller KW,Elsner P,Maibach HI.Intrinsic and extrinsic factors in skin ageing:A review.Int J Cosmet Sci 2008;30(2):87-95.
[3]Bertuccelli G,Zerbinati N,Marcellino M,Nanda Kumar NS,He F,Tsepakolenko V,et al.Effect of a quality-controlled fermented nutraceutical on skin aging markers:An antioxidant-control,double-blind study.Exp Ther Med 2016;11(3):909-916.
[4]Dvorankova B,Szabo P,Lacina L,Gal P,Uhrova J,Zima T,et al.Human galectins induce conversion of dermal fibroblasts into myofibroblasts and production of extracellular matrix:Potential application in tissue engineering and wound repair.Cells Tissues Organs 2011;194(6):469-480.
[5]Fisher GJ,Quan T,Purohit T,Shao Y,Cho MK,He T,et al.Collagen fragmentation promotes oxidative stress and elevates matrix metalloproteinase-1 in fibroblasts in aged human skin.Am J Pathol 2009;174(1):101-114.
[6]Pittayapruek P,Meephansan J,Prapapan O,Komine M,Ohtsuki M.Role of matrix metalloproteinases in photoaging and photocarcinogenesis.Int JMol Sci 2016;17(6):868.
[7]Hideg E,Jansen MA,Strid A.UV-B exposure,ROS,and stress:Inseparable companions or loosely linked associates?Trends Plant Sci2013;18(2):107-115.
[8]Zhu X,Li N,Wang Y,Ding L,Chen H,Yu Y,et al.Protective effects of quercetin on UVB irradiation-induced cytotoxicity through ROSclearance in keratinocyte cells.Oncol Rep 2017;37(1):209-218.
[9]Cai L,Wu CD.Compounds from Syzygium aromaticum possessing growth inhibitory activity against oral pathogens.J Nat Prod 1996;59(10):987-990.
[10]Dorman HJ,Deans SG.Antimicrobial agents from plants:Antibacterial activity of plant volatile oils.J Appl Microbiol 2000;88(2):308-316.
[11]Jirovetz L,Buchbauer G,Stoilova I,Stoyanova A,Krastanov A,Schmidt E.Chemical composition and antioxidant properties of clove leaf essential oil.J Agric Food Chem 2006;54(17):6303-6307.
[12]Lee KG,Shibamoto T.Inhibition of malonaldehyde formation from blood plasma oxidation by aroma extracts and aroma components isolated from clove and eucalyptus.Food Chem Toxicol 2001;39(12):1199-1204.
[13]Tajuddin,Ahmad S,Latif A,Qasmi IA.Effect of 50%ethanolic extract of Syzygium aromaticum(L.)Merr.&Perry.(clove)on sexual behaviour of normal male rats.BMC Complement Altern Med 2004;4:17.
[14]Rodrigues TG,Fernandes A Jr,Sousa JP,Bastos JK,Sforcin JM.In vitro and in vivo effects of clove on pro-inflammatory cytokines production by macrophages.Nat Prod Res 2009;23(4):319-326.
[15]Adefegha SA,Oboh G.In vitro inhibition activity of polyphenol-rich extracts from Syzygium aromaticum(L.)Merr.&Perry(Clove)buds against carbohydrate hydrolyzing enzymes linked to type 2 diabetes and Fe(2+)-induced lipid peroxidation in rat pancreas.Asian Pac J Trop Biomed 2012;2(10):774-781.
[16]Chang BY,Lee DS,Lee JK,Kim YC,Cho HK,Kim SY.Protective activity of kudzu(Pueraria thunbergiana)vine on chemically-induced hepatotoxicity:In vitro and in vivo studies.BMC Complement Altern Med2016;16(1):39.
[17]Manivasagan P,Alam MS,Kang KH,Kwak M,Kim SK.Extracellular synthesis of gold bionanoparticles by Nocardiopsis sp.and evaluation of its antimicrobial,antioxidant and cytotoxic activities.Bioprocess Biosyst Eng 2015;38(6):1167-1177.
[18]Ishii Y,Sugimoto S,Izawa N,Sone T,Chiba K,Miyazaki K.Oral administration of Bifidobacterium breve attenuates UV-induced barrier perturbation and oxidative stress in hairless mice skin.Arch Dermatol Res2014;306(5):467-473.
[19]Stergiou PY,Foukis A,Gkini OA,Bieth JG,Papamichael EM.Kinetic and computational analysis of the reversible inhibition of porcine pancreatic elastase:A structural and mechanistic approach.J Enzyme Inhib Med Chem 2016;31(3):131-139.
[20]Liao KH,Lin YS,Macosko CW,Haynes CL.Cytotoxicity of graphene oxide and graphene in human erythrocytes and skin fibroblasts.ACS Appl Mater Interfaces 2011;3(7):2607-2615.
[21]Amato B,Coretti G,Compagna R,Amato M,Buffone G,Gigliotti D,et al.Role of matrix metalloproteinases in non-healing venous ulcers.Int Wound J 2015;12(6):641-645.
[22]Wilmink GJ,Roth CL,Ibey BL,Ketchum N,Bernhard J,Cerna CZ,et al.Identification of microRNAs associated with hyperthermia-induced cellular stress response.Cell Stress Chaperones 2010;15(6):1027-1038.
[23]Mohammadi MH,Heidary Araghi B,Beydaghi V,Geraili A,Moradi F,Jafari P,et al.Skin diseases modeling using combined tissue engineering and microfluidic technologies.Adv Healthc Mater 2016;5(19):2459-2480.
[24]Tracy LE,Minasian RA,Caterson EJ.Extracellular matrix and dermal fibroblast function in the healing wound.Adv Wound Care(New Rochelle)2016;5(3):119-136.
[25]Saldin LT,Cramer MC,Velankar SS,White LJ,Badylak SF.Extracellular matrix hydrogels from decellularized tissues:Structure and function.Acta Biomaterialia 2017;49:1-15.
[26]Kang TH,Park HM,Kim YB,Kim H,Kim N,Do JH,et al.Effects of red ginseng extract on UVB irradiation-induced skin aging in hairless mice.J Ethnopharmacol 2009;123(3):446-451.
[27]Kim DB,Shin GH,Kim JM,Kim YH,Lee JH,Lee JS,et al.Antioxidant and anti-ageing activities of citrus-based juice mixture.Food Chem 2016;194:920-927.
[28]Gill SS,Tuteja N.Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants.Plant Physiol Biochem 2010;48(12):909-930.
[29]Wang L,Ding L,Yu Z,Zhang T,Ma S,Liu J.Intracellular ROSscavenging and antioxidant enzyme regulating capacities of corn gluten meal-derived antioxidant peptides in HepG2 cells.Food Res Int 2016;90:33-41.
[30]Razafimamonjison G,Jahiel M,Duclos T,Ramanoelina P,Fawbush F,Danthu P.Bud,leaf and stem essential oil composition of Syzygium aromaticum from Madagascar,Indonesia and Zanzibar.Int J Basic Appl Sci 2014;3(3):224-233.
[31]Santoro GF,Cardoso MG,Guimar?es LG,Mendon?a LZ,Soares MJ.Trypanosoma cruzi:Activity of essential oils from Achillea millefolium L.,Syzygium aromaticum L.and Ocimum basilicum L.on epimastigotes and trypomastigotes.Exp Parasitol 2007;116(3):283-290.
[32]Ajiboye TO,Mohammed AO,Bello SA,Yusuf II,Ibitoye OB,Muritala HF,et al.Antibacterial activity of Syzygium aromaticum seed:Studies on oxidative stress biomarkers and membrane permeability.Microb Pathog2016;95:208-215.
[33]Saikumari D,Rani SKS,Saxena N.Antibacterial activity of Syzigium aromaticum L.(Clove).Int J Curr Microbiol Appl Sci 2016;5(11):6.
[34]Mittal M,Gupta N,Parashar P,Mehra V,Khatri M.Phytochemical evaluation and pharmacological activity of Syzygium aromaticum:Acomprehensive review.Int J Pharm Pharmaceut Sci 2014;6(8):67-72.
[35]Chaieb K,Hajlaoui H,Zmantar T,Kahla-Nakbi AB,Rouabhia M,Mahdouani K,et al.The chemical composition and biological activity of clove essential oil,Eugenia caryophyllata(Syzigium aromaticum L.Myrtaceae):A short review.Phytother Res 2007;21(6):501-506.
[36]Aziz J,Shezali H,Radzi Z,Yahya NA,Abu Kassim NH,Czernuszka J,et al.Molecular mechanisms of stress-responsive changes in collagen and elastin networks in skin.Skin Pharmacol Physiol 2016;29(4):190-203.
[37]Chung JH,Seo JY,Choi HR,Lee MK,Youn CS,Rhie G,et al.Modulation of skin collagen metabolism in aged and photoaged human skin in vivo.J Invest Dermatol 2001;117(5):1218-1224.
[38]Fujiwara M,Muragaki Y,Ooshima A.Keloid-derived fibroblasts show increased secretion of factors involved in collagen turnover and depend on matrix metalloproteinase for migration.Br J Dermatol 2005;153(2):295-300.
[39]Roh E,Kim JE,Kwon JY,Park JS,Bode AM,Dong Z,et al.Molecular mechanisms of green tea polyphenols with protective effects against skin photoaging.Crit Rev Food Sci Nutr 2017;57(8):1631-1637.
[40]Sela-Passwell N,Rosenblum G,Shoham T,Sagi I.Structural and functional bases for allosteric control of MMP activities:Can it pave the path for selective inhibition?Biochim Biophys Acta 2010;1803(1):29-38.
[41]Kim J,Kim MB,Yun JG,Hwang JK.Protective effects of standardized Siegesbeckia glabrescens extract and its active compound kirenol against UVB-induced photoaging through inhibition of MAPK/NF-κB pathways.J Microbiol Biotechnol 2017;27(2):242-250.
[42]Jab?ońska-Trypu?A,Matejczyk M,Rosochacki S.Matrix metalloproteinases(MMPs),the main extracellular matrix(ECM)enzymes in collagen degradation,as a target for anticancer drugs.JEnzyme Inhib Med Chem 2016;31(sup1):177-183.
[2]Farage MA,Miller KW,Elsner P,Maibach HI.Intrinsic and extrinsic factors in skin ageing:A review.Int J Cosmet Sci 2008;30(2):87-95.
[3]Bertuccelli G,Zerbinati N,Marcellino M,Nanda Kumar NS,He F,Tsepakolenko V,et al.Effect of a quality-controlled fermented nutraceutical on skin aging markers:An antioxidant-control,double-blind study.Exp Ther Med 2016;11(3):909-916.
[4]Dvorankova B,Szabo P,Lacina L,Gal P,Uhrova J,Zima T,et al.Human galectins induce conversion of dermal fibroblasts into myofibroblasts and production of extracellular matrix:Potential application in tissue engineering and wound repair.Cells Tissues Organs 2011;194(6):469-480.
[5]Fisher GJ,Quan T,Purohit T,Shao Y,Cho MK,He T,et al.Collagen fragmentation promotes oxidative stress and elevates matrix metalloproteinase-1 in fibroblasts in aged human skin.Am J Pathol 2009;174(1):101-114.
[6]Pittayapruek P,Meephansan J,Prapapan O,Komine M,Ohtsuki M.Role of matrix metalloproteinases in photoaging and photocarcinogenesis.Int JMol Sci 2016;17(6):868.
[7]Hideg E,Jansen MA,Strid A.UV-B exposure,ROS,and stress:Inseparable companions or loosely linked associates?Trends Plant Sci2013;18(2):107-115.
[8]Zhu X,Li N,Wang Y,Ding L,Chen H,Yu Y,et al.Protective effects of quercetin on UVB irradiation-induced cytotoxicity through ROSclearance in keratinocyte cells.Oncol Rep 2017;37(1):209-218.
[9]Cai L,Wu CD.Compounds from Syzygium aromaticum possessing growth inhibitory activity against oral pathogens.J Nat Prod 1996;59(10):987-990.
[10]Dorman HJ,Deans SG.Antimicrobial agents from plants:Antibacterial activity of plant volatile oils.J Appl Microbiol 2000;88(2):308-316.
[11]Jirovetz L,Buchbauer G,Stoilova I,Stoyanova A,Krastanov A,Schmidt E.Chemical composition and antioxidant properties of clove leaf essential oil.J Agric Food Chem 2006;54(17):6303-6307.
[12]Lee KG,Shibamoto T.Inhibition of malonaldehyde formation from blood plasma oxidation by aroma extracts and aroma components isolated from clove and eucalyptus.Food Chem Toxicol 2001;39(12):1199-1204.
[13]Tajuddin,Ahmad S,Latif A,Qasmi IA.Effect of 50%ethanolic extract of Syzygium aromaticum(L.)Merr.&Perry.(clove)on sexual behaviour of normal male rats.BMC Complement Altern Med 2004;4:17.
[14]Rodrigues TG,Fernandes A Jr,Sousa JP,Bastos JK,Sforcin JM.In vitro and in vivo effects of clove on pro-inflammatory cytokines production by macrophages.Nat Prod Res 2009;23(4):319-326.
[15]Adefegha SA,Oboh G.In vitro inhibition activity of polyphenol-rich extracts from Syzygium aromaticum(L.)Merr.&Perry(Clove)buds against carbohydrate hydrolyzing enzymes linked to type 2 diabetes and Fe(2+)-induced lipid peroxidation in rat pancreas.Asian Pac J Trop Biomed 2012;2(10):774-781.
[16]Chang BY,Lee DS,Lee JK,Kim YC,Cho HK,Kim SY.Protective activity of kudzu(Pueraria thunbergiana)vine on chemically-induced hepatotoxicity:In vitro and in vivo studies.BMC Complement Altern Med2016;16(1):39.
[17]Manivasagan P,Alam MS,Kang KH,Kwak M,Kim SK.Extracellular synthesis of gold bionanoparticles by Nocardiopsis sp.and evaluation of its antimicrobial,antioxidant and cytotoxic activities.Bioprocess Biosyst Eng 2015;38(6):1167-1177.
[18]Ishii Y,Sugimoto S,Izawa N,Sone T,Chiba K,Miyazaki K.Oral administration of Bifidobacterium breve attenuates UV-induced barrier perturbation and oxidative stress in hairless mice skin.Arch Dermatol Res2014;306(5):467-473.
[19]Stergiou PY,Foukis A,Gkini OA,Bieth JG,Papamichael EM.Kinetic and computational analysis of the reversible inhibition of porcine pancreatic elastase:A structural and mechanistic approach.J Enzyme Inhib Med Chem 2016;31(3):131-139.
[20]Liao KH,Lin YS,Macosko CW,Haynes CL.Cytotoxicity of graphene oxide and graphene in human erythrocytes and skin fibroblasts.ACS Appl Mater Interfaces 2011;3(7):2607-2615.
[21]Amato B,Coretti G,Compagna R,Amato M,Buffone G,Gigliotti D,et al.Role of matrix metalloproteinases in non-healing venous ulcers.Int Wound J 2015;12(6):641-645.
[22]Wilmink GJ,Roth CL,Ibey BL,Ketchum N,Bernhard J,Cerna CZ,et al.Identification of microRNAs associated with hyperthermia-induced cellular stress response.Cell Stress Chaperones 2010;15(6):1027-1038.
[23]Mohammadi MH,Heidary Araghi B,Beydaghi V,Geraili A,Moradi F,Jafari P,et al.Skin diseases modeling using combined tissue engineering and microfluidic technologies.Adv Healthc Mater 2016;5(19):2459-2480.
[24]Tracy LE,Minasian RA,Caterson EJ.Extracellular matrix and dermal fibroblast function in the healing wound.Adv Wound Care(New Rochelle)2016;5(3):119-136.
[25]Saldin LT,Cramer MC,Velankar SS,White LJ,Badylak SF.Extracellular matrix hydrogels from decellularized tissues:Structure and function.Acta Biomaterialia 2017;49:1-15.
[26]Kang TH,Park HM,Kim YB,Kim H,Kim N,Do JH,et al.Effects of red ginseng extract on UVB irradiation-induced skin aging in hairless mice.J Ethnopharmacol 2009;123(3):446-451.
[27]Kim DB,Shin GH,Kim JM,Kim YH,Lee JH,Lee JS,et al.Antioxidant and anti-ageing activities of citrus-based juice mixture.Food Chem 2016;194:920-927.
[28]Gill SS,Tuteja N.Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants.Plant Physiol Biochem 2010;48(12):909-930.
[29]Wang L,Ding L,Yu Z,Zhang T,Ma S,Liu J.Intracellular ROSscavenging and antioxidant enzyme regulating capacities of corn gluten meal-derived antioxidant peptides in HepG2 cells.Food Res Int 2016;90:33-41.
[30]Razafimamonjison G,Jahiel M,Duclos T,Ramanoelina P,Fawbush F,Danthu P.Bud,leaf and stem essential oil composition of Syzygium aromaticum from Madagascar,Indonesia and Zanzibar.Int J Basic Appl Sci 2014;3(3):224-233.
[31]Santoro GF,Cardoso MG,Guimar?es LG,Mendon?a LZ,Soares MJ.Trypanosoma cruzi:Activity of essential oils from Achillea millefolium L.,Syzygium aromaticum L.and Ocimum basilicum L.on epimastigotes and trypomastigotes.Exp Parasitol 2007;116(3):283-290.
[32]Ajiboye TO,Mohammed AO,Bello SA,Yusuf II,Ibitoye OB,Muritala HF,et al.Antibacterial activity of Syzygium aromaticum seed:Studies on oxidative stress biomarkers and membrane permeability.Microb Pathog2016;95:208-215.
[33]Saikumari D,Rani SKS,Saxena N.Antibacterial activity of Syzigium aromaticum L.(Clove).Int J Curr Microbiol Appl Sci 2016;5(11):6.
[34]Mittal M,Gupta N,Parashar P,Mehra V,Khatri M.Phytochemical evaluation and pharmacological activity of Syzygium aromaticum:Acomprehensive review.Int J Pharm Pharmaceut Sci 2014;6(8):67-72.
[35]Chaieb K,Hajlaoui H,Zmantar T,Kahla-Nakbi AB,Rouabhia M,Mahdouani K,et al.The chemical composition and biological activity of clove essential oil,Eugenia caryophyllata(Syzigium aromaticum L.Myrtaceae):A short review.Phytother Res 2007;21(6):501-506.
[36]Aziz J,Shezali H,Radzi Z,Yahya NA,Abu Kassim NH,Czernuszka J,et al.Molecular mechanisms of stress-responsive changes in collagen and elastin networks in skin.Skin Pharmacol Physiol 2016;29(4):190-203.
[37]Chung JH,Seo JY,Choi HR,Lee MK,Youn CS,Rhie G,et al.Modulation of skin collagen metabolism in aged and photoaged human skin in vivo.J Invest Dermatol 2001;117(5):1218-1224.
[38]Fujiwara M,Muragaki Y,Ooshima A.Keloid-derived fibroblasts show increased secretion of factors involved in collagen turnover and depend on matrix metalloproteinase for migration.Br J Dermatol 2005;153(2):295-300.
[39]Roh E,Kim JE,Kwon JY,Park JS,Bode AM,Dong Z,et al.Molecular mechanisms of green tea polyphenols with protective effects against skin photoaging.Crit Rev Food Sci Nutr 2017;57(8):1631-1637.
[40]Sela-Passwell N,Rosenblum G,Shoham T,Sagi I.Structural and functional bases for allosteric control of MMP activities:Can it pave the path for selective inhibition?Biochim Biophys Acta 2010;1803(1):29-38.
[41]Kim J,Kim MB,Yun JG,Hwang JK.Protective effects of standardized Siegesbeckia glabrescens extract and its active compound kirenol against UVB-induced photoaging through inhibition of MAPK/NF-κB pathways.J Microbiol Biotechnol 2017;27(2):242-250.
[42]Jab?ońska-Trypu?A,Matejczyk M,Rosochacki S.Matrix metalloproteinases(MMPs),the main extracellular matrix(ECM)enzymes in collagen degradation,as a target for anticancer drugs.JEnzyme Inhib Med Chem 2016;31(sup1):177-183.