microRNA-199a:子宫内膜异位症中的关键因子
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摘要
子宫内膜异位症是一种常见的雌激素依赖性疾病,其特征在于子宫外的子宫内膜组织的生长。微小RNA最近已被确定为调节靶基因表达的调节因子,并被认为参与子宫内膜异位症的发展。miR-199a在子宫内膜异位症患者子宫内膜中的表达水平较低,与正常女性子宫内膜相比,卵巢子宫内膜瘤的表达水平甚至更低。MiR-199a在子宫内膜间质细胞(ESC)中靶向并抑制IkappaB激酶β(IKKb),随着IKKb的减少,miR-199a抑制了ESC中的核因子-κB(NF-κB)途径激活和白细胞介素-8(IL-8)的产生。miR-199a通过血管内皮生长因子A(VEGF-A)途径抑制部分缺氧条件下胚胎干细胞的血管生成潜能。所有这些发现表明,在子宫内膜异位症中下调的miR-199a部分通过IKK/NF-κB途径,VEGF-A途径抑制和降低的IL-8表达减弱了体外胚胎干细胞的粘附侵袭和血管生成能力。因此,miR-199a可能在子宫内膜异位症的发病机制中发挥关键作用,并可能成为该疾病的潜在治疗靶点。
Endometriosis is a common estrogen-dependent disorder characterized by the growth of endometrial tissue outside the uterus.MicroRNAs have recently been identified as regulators of target gene expression and are thought to be involved in the development and progression of endometriosis.In patients with endometriosis,the expression levels of miR-199a in the endometrium in situ are low,and the expression levels of ovarian endometrioma are even lower than those in normal female endometrium.MiR-199a targets and inhibits IkappaB kinase(IKKb) in endometrial stromal cells(ESC).With the decrease of IKKb,miR-199a inhibits the nuclear factor-κB(NF-κB) pathway activation and the production of interleukin-8(IL-8) in ESC.Moreover Mir-199a inhibits the angiogenic potential of embryonic stem cells under partial hypoxia through the vascular endothelial growth factor A(VEGF-A) pathway.All these findings suggest that the down-regulated miR-199a portion of endometriosis reduces the adhesion invasion and angiogenesis of embryonic stem cells in vitro by inhibiting and decreasing IL-8 expression through the IKK/NF-κB pathway and VEGF-A pathway.Therefore,miA-199a may play a key role in the pathogenesis of endometriosis and may be a potential therapeutic target for this disease.
Endometriosis is a common estrogen-dependent disorder characterized by the growth of endometrial tissue outside the uterus.MicroRNAs have recently been identified as regulators of target gene expression and are thought to be involved in the development and progression of endometriosis.In patients with endometriosis,the expression levels of miR-199a in the endometrium in situ are low,and the expression levels of ovarian endometrioma are even lower than those in normal female endometrium.MiR-199a targets and inhibits IkappaB kinase(IKKb) in endometrial stromal cells(ESC).With the decrease of IKKb,miR-199a inhibits the nuclear factor-κB(NF-κB) pathway activation and the production of interleukin-8(IL-8) in ESC.Moreover Mir-199a inhibits the angiogenic potential of embryonic stem cells under partial hypoxia through the vascular endothelial growth factor A(VEGF-A) pathway.All these findings suggest that the down-regulated miR-199a portion of endometriosis reduces the adhesion invasion and angiogenesis of embryonic stem cells in vitro by inhibiting and decreasing IL-8 expression through the IKK/NF-κB pathway and VEGF-A pathway.Therefore,miA-199a may play a key role in the pathogenesis of endometriosis and may be a potential therapeutic target for this disease.
引文
1 Wright Kristeena Ray,Mitchell Brenda,Santanam Nalini.Redox regulation of microRNAs in endometriosis-associated pain.Redox Biol,2017,12:956-966.
2 Eliasson Lena.The small RNA miR-375 - a pancreatic islet abundant miRNA with multiple roles in endocrine beta cell function.Mol Cell Endocrinol,2017,456:95-101.
3 Heidemann J,Ogawa H,Dwinell MB,et al.Angiogenic effects of interleukin 8 (CXCL8)in human intestinal microvascular endothelial cells are mediated by CXCR2.J Biol Chem,2003,278:8508-8515.
4 Maged Ahmed M,Deeb Wesam S,El Amir Azza,et al.Diagnostic accuracy of serum miR-122 and miR-199a in women with endometriosis.Int J Gynaecol Obstet,2018,141:14-19.
5 Xu Feng,Liu Qian,Chen Luying,et al.Genome-wide identification of soybean microRNAs and their targets reveals their organ-specificity and responses to phosphate starvation.BMC Genomics,2013,14:66.
6 Hammond Scott M.MicroRNA therapeutics:a new niche for antisense nucleic acids.Trends Mol Med,2006,12:99-101.
7 Yuan Jian,Xiao Gelei,Peng Gang,et al.MiRNA-125a-5p inhibits glioblastoma cell proliferation and promotes cell differentiation by targeting TAZ..Biochem.Biophys.Res.Commun,2015,457:171-176.
8 Dai Lan,Gu Liying,Di Wen.MiR-199a attenuates endometrial stromal cell invasiveness through suppression of the IKKβ/NF-κB pathway and reduced interleukin-8 expression.Mol Hum Reprod,2012,18:136-145.
9 Song Jie,Li Na,Xia Yang,et al.Arctigenin Confers Neuroprotection Against Mechanical Trauma Injury in Human Neuroblastoma SH-SY5Y Cells by Regulating miRNA-16 and miRNA-199a Expression to Alleviate Inflammation.J Mol Neurosci,2016,60:115-129.
10 Kuokkanen S,Chen B,Ojalvo L,et al.Genomic profILing of microRNAs and messenger RNAs reveals hormonal regulation in microRNA expression in human endometrium.Biol Reprod,2010,82:791-801.
11 陆丹,贺青蓉,秦炜,等.miRNA 199a、miRNA 126在子宫内膜异位症组织中的表达及意义.山东医药,2014,54:40-41.
12 Wu Can,Lv Chuan,Chen Fenqin,et al.The function of miR-199a-5p/Klotho regulating TLR4/NF-κB p65/NGAL pathways in rat mesangial cells cultured with high glucose and the mechanism.Mol Cell Endocrinol,2015,417:84-93.
13 Dai Lan,Lou Weihua,Zhu Jie,et al.MiR-199a inhibits the angiogenic potential of endometrial stromal cells under hypoxia by targeting HIF-1α/VEGF pathway.Int J Clin Exp Pathol,2015,8:4735-4744.
14 Celik O,Ersahin A,Acet M,et al.Disulfiram,as a candidate NF-κB and proteasome inhibitor,prevents endometriotic implant growing in a rat model of endometriosis.Eur Rev Med Pharmacol Sci,2016,20:4380-4389.
15 Chang Kai-Kai,Liu Li-Bing,Li Hui,et al.TSLP induced by estrogen stimulates secretion of MCP-1 and IL-8 and growth of human endometrial stromal cells through JNK and NF-κB signal pathways.Int J Clin Exp Pathol,2014,7:1889-1899.
16 Tao Xiang,Xie Yan,Wang Li,et al.The expression of Cox-2,NF-κB,and VEGF in ectopic endometrial tissues within fallopian tubes suggests different etiologies.Int J Gynecol Pathol,2014,33:411-417.
17 Yamanaka Kaoruko,Xu Bing,Suganuma Izumi,et al.Dienogest inhibits aromatase and cyclooxygenase-2 expression and prostaglandin E production in human endometriotic stromal cells in spheroid culture.Fertil Steril,2012,97:477-482.
18 Wang Xue-Rong,Hao Hong-Guang,Chu Lan.Glycyrrhizin inhibits LPS-induced inflammatory mediator production in endometrial epithelial cells.Microb Pathog,2017,109:110-113.
19 Takai Eri,Taniguchi Fuminori,Nakamura Kazuomi,et al.Parthenolide reduces cell proliferation and prostaglandin E2 [corrected] in human endometriotic stromal cells and inhibits development of endometriosis in the murine model.Fertil Steril,2013,100:1170-1178.
20 Song Tao,Zhang Xu,Yang Guoqiang,et al.Decrement of miR-199a-5p contributes to the tumorigenesis of bladder urothelial carcinoma by regulating MLK3/NF-κB pathway.Am J Transl Res,2015,7:2786-2794.
21 Jiang Yuan,Zhu Ying,Wang Xinxin,et al.Temporal regulation of HIF-1 and NF-κB in hypoxic hepatocarcinoma cells.Oncotarget,2015,6:9409-9419.
22 Bo Zhang,Xiang Qian,Shan-Ming Ruan,et al.Investigation on Molecular Mechanism of Fibroblast Regulation and the Treatment of Recurrent Oral Ulcer by Shuizhongcao Granule-Containing Serum.Evid Based Complement Alternat Med,2015,2015:324091.
23 Li Yue,Wang Xiaoyan,Wang Xishuang,et al.PDCD4 suppresses proliferation,migration,and invasion of endometrial cells by inhibiting autophagy and NF-κB/MMP2/MMP9 signal pathway.Biol Reprod,2018,99:360-372.
24 Esposito Evangelina,Aldrees Sultan,Mastromonaco Christina,et al.Evaluation of nicotinamide as an anti-inflammatory and anti-angiogenic agent in uveal melanoma cell lines.Arq Bras Oftalmol,2017,80:74-77.
25 Mulayim Naciye,Savlu Asim,Guzeloglu-Kayisli Ozlem,et al.Regulation of endometrial stromal cell matrix metalloproteinase activity and invasiveness by interleukin-8.Fertil Steril,2004,null:904-911.
26 Garcia-Velasco Juan A,Mulayim Naciye,Kayisli Umit A,et al.Elevated soluble Fas ligand levels may suggest a role for apoptosis in women with endometriosis.Fertil Steril,2002,78:855-859.
27 Sharpe-Timms KL,Nabli H,Zimmer RL,et al.Inflammatory cytokines differentially up-regulate human endometrial haptoglobin production in women with endometriosis.Hum Reprod ,2010,25:1241-1250.
28 Brenner Robert M,Nayak Nihar R,Slayden Ov D,et al.Premenstrual and menstrual changes in the macaque and human endometrium:relevance to endometriosis.Ann N Y Acad Sci,2002,955:60-74;discussion 86-88,396-406.
29 Luo Xue-zhen,Zhou Wen-jie,Tao Yu,et al.TLR4 Activation Promotes the Secretion of IL-8 Which Enhances the Invasion and Proliferation of Endometrial Stromal Cells in an Autocrine Manner via the FAK Signal Pathway.Am J Reprod Immunol ,2015,74:467-479.
30 Selam Belgin,Kayisli Umit A,Garcia-Velasco Juan A,et al.Regulation of fas ligand expression by IL-8 in human endometrium.J Clin Endocrinol Metab,2002,87:3921-3927.
31 Kim Ji-Yeon,Lee Dong-Hyung,Joo Jong-Kil,et al.Effects of peritoneal fluid from endometriosis patients on interferon-gamma-induced protein-10 (CXCL10)and interleukin-8 (CXCL8)released by neutrophils and CD4+ T cells.Am J Reprod Immunol,2009,62:128-138.
32 Cousins Fiona L,O Dorien F,Gargett Caroline E.Endometrial stem/progenitor cells and their role in the pathogenesis of endometriosis.Best Pract Res Clin Obstet Gynaecol,2018,50:27-38.
33 易秋红.MiRNA-199a、MMP-9在子宫内膜异位症患者中的表达及意义.山西医科大学,2016.
34 Izumi Gentaro,Koga Kaori,Takamura Masashi,et al.Involvement of immune cells in the pathogenesis of endometriosis.J Obstet Gynaecol Res,2018,44:191-198.
35 Wang Dan,Jiang YIling,Yang Xiaoxin,et al.6-Shogaol reduces progression of experimental endometriosis and regulation of VGEF and inhibition of COX-2 and PGE2-mediated inflammatory responses.Korean J Physiol Pharmacol,2018,22:627-636.
36 Marí-Alexandre Josep,Barceló-Molina Moisés,Belmonte-López Elisa,et al.Micro-RNA profILe and proteins in peritoneal fluid from women with endometriosis:their relationship with sterILity.FertIL SterIL,2018,109:675-684.e2.
37 Agrawal Swati,Tapmeier Thomas,Rahmioglu Nilufer,et al.The miRNA Mirage:How Close Are We to Finding a Non-Invasive Diagnostic Biomarker in Endometriosis A Systematic Review.Int J Mol Sci,2018,19:undefined.
38 Wu J,Cui SH,Li HZ,et al.Ultrasound diagnosis in gynecological acute abdomen.J Biol Regul Homeost Agents,2016,30:211-217.
39 Hsu Chia-Yi,Hsieh Tsung-Hua,Tsai Cheng-Fang,et al.Synthetic Steroid Hormones Regulated Cell Proliferation Through MicroRNA-34a-5p in Human Ovarian Endometrioma.Biol Reprod,2016,94:60.
40 Wang Wen-Tao,Zhao Ya-Nan,Han Bo-Wei,et al.Circulating microRNAs identified in a genome-wide serum microRNA expression analysis as noninvasive biomarkers for endometriosis.J Clin Endocrinol Metab,2013,98:281-289.
41 Young Vicky J,Ahmad Syed F,Brown Jeremy K,et al.Peritoneal VEGF-A expression is regulated by TGF-β1 through an ID1 pathway in women with endometriosis.Sci Rep,2015,5:16859.
42 Hall M,Gourley C,McNeish I,et al.Targeted anti-vascular therapies for ovarian cancer:current evidence.Br J Cancer,2013,108:250-258.
43 Moon Jeong Min,Chung Youn-Jee,Chae Boah,et al.Effect of mistletoe on endometrial stromal cell survival and vascular endothelial growth factor expression in patients with endometriosis.Int J Med Sci,2018,15:1530-1536.
44 Pourjafar M,Saidijam M,Mansouri K,et al.All-trans retinoic acid preconditioning enhances proliferation,angiogenesis and migration of mesenchymal stem cell in vitro and enhances wound repair in vivo.Cell Prolif,2017,50:undefined.
45 Yerlikaya Gülen,Balendran Sukirthini,Prstling Katharina,et al.Comprehensive study of angiogenic factors in women with endometriosis compared to women without endometriosis.Eur J Obstet Gynecol Reprod Biol,2016,204:88-98.
46 Dai Lan,Gu Li-ying,Zhu Jie,et al.Regulation of microRNA-199a on adhesion,migration and invasion ability of human endometrial stromal cells.Zhonghua Fu Chan Ke Za Zhi,2011,46:817-821.
47 Hsu Chia-Yi,Hsieh Tsung-Hua,Tsai Cheng-Fang,et al.miRNA-199a-5p regulates VEGFA in endometrial mesenchymal stem cells and contributes to the pathogenesis of endometriosis.J Pathol,2014,232:330-343.
48 Lin Shih-Chieh,Wang Chih-Chuan,Wu Meng-Hsing,et al.Hypoxia-induced microRNA-20a expression increases ERK phosphorylation and angiogenic gene expression in endometriotic stromal cells.J Clin Endocrinol Metab,2012,97:E1515-1523.
49 Hou Jingying,Wang Lingyun,Wu Quanhua,et al.Long noncoding RNA H19 upregulates vascular endothelial growth factor A to enhance mesenchymal stem cells survival and angiogenic capacity by inhibiting miR-199a-5p.Stem Cell Res Ther,2018,9:109.
50 Kim Ji-Hyun,Yang Yeong-In,Lee Kyung-Tae,et al.Costunolide induces apoptosis in human endometriotic cells through inhibition of the prosurvival Akt and nuclear factor kappa B signaling pathway.Biol Pharm Bull,2011,34:580-585.
51 Xing Yu,Li Ling.Gastrodin protects rat cardiomyocytes H9c2 from hypoxia-induced injury by up-regulation of microRNA-21.Int J Biochem Cell Biol,2019,109:8-16.
52 Hirata Yukihiro,Murai Noriyuki,Yanaihara Nozomu,et al.MicroRNA-21 is a candidate driver gene for 17q23-25 amplification in ovarian clear cell carcinoma.BMC Cancer,2014,14:799.
53 Lijie He,Shiren Sun,Hanmin Wang,et al.miRNA-199a/214 Cluster Enhances Fibrosis of Human Peritoneal Mesothelial Cells Regulated by SRF.Hong Kong Journal of Nephrology,2015,17:412-421.
54 Er Tze-Kiong,Su Yu-Fa,Wu Chun-Chieh,et al.Targeted next-generation sequencing for molecular diagnosis of endometriosis-associated ovarian cancer.J Mol Med,2016,94:835-847.
55 林勤,郑轩,杨华静,等.MiRNA-199a-3p通过mTOR通路与阿霉素联合抑制子宫内膜癌细胞的增殖和促凋亡作用.现代妇产科进展,2014,23:673-676.
56 Matsuzaki S,Canis M,Pouly JL,et al.Relationship between delay of surgical diagnosis and severity of disease in patients with symptomatic deep infILtrating endometriosis.FertIL SterIL,2006,86:1314-1316.
57 Wang Fang,Wang Hongxia,Jin Danting,et al.Serum miR-17,IL-4,and IL-6 levels for diagnosis of endometriosis.Medicine (Baltimore),2018,97:e10853.
2 Eliasson Lena.The small RNA miR-375 - a pancreatic islet abundant miRNA with multiple roles in endocrine beta cell function.Mol Cell Endocrinol,2017,456:95-101.
3 Heidemann J,Ogawa H,Dwinell MB,et al.Angiogenic effects of interleukin 8 (CXCL8)in human intestinal microvascular endothelial cells are mediated by CXCR2.J Biol Chem,2003,278:8508-8515.
4 Maged Ahmed M,Deeb Wesam S,El Amir Azza,et al.Diagnostic accuracy of serum miR-122 and miR-199a in women with endometriosis.Int J Gynaecol Obstet,2018,141:14-19.
5 Xu Feng,Liu Qian,Chen Luying,et al.Genome-wide identification of soybean microRNAs and their targets reveals their organ-specificity and responses to phosphate starvation.BMC Genomics,2013,14:66.
6 Hammond Scott M.MicroRNA therapeutics:a new niche for antisense nucleic acids.Trends Mol Med,2006,12:99-101.
7 Yuan Jian,Xiao Gelei,Peng Gang,et al.MiRNA-125a-5p inhibits glioblastoma cell proliferation and promotes cell differentiation by targeting TAZ..Biochem.Biophys.Res.Commun,2015,457:171-176.
8 Dai Lan,Gu Liying,Di Wen.MiR-199a attenuates endometrial stromal cell invasiveness through suppression of the IKKβ/NF-κB pathway and reduced interleukin-8 expression.Mol Hum Reprod,2012,18:136-145.
9 Song Jie,Li Na,Xia Yang,et al.Arctigenin Confers Neuroprotection Against Mechanical Trauma Injury in Human Neuroblastoma SH-SY5Y Cells by Regulating miRNA-16 and miRNA-199a Expression to Alleviate Inflammation.J Mol Neurosci,2016,60:115-129.
10 Kuokkanen S,Chen B,Ojalvo L,et al.Genomic profILing of microRNAs and messenger RNAs reveals hormonal regulation in microRNA expression in human endometrium.Biol Reprod,2010,82:791-801.
11 陆丹,贺青蓉,秦炜,等.miRNA 199a、miRNA 126在子宫内膜异位症组织中的表达及意义.山东医药,2014,54:40-41.
12 Wu Can,Lv Chuan,Chen Fenqin,et al.The function of miR-199a-5p/Klotho regulating TLR4/NF-κB p65/NGAL pathways in rat mesangial cells cultured with high glucose and the mechanism.Mol Cell Endocrinol,2015,417:84-93.
13 Dai Lan,Lou Weihua,Zhu Jie,et al.MiR-199a inhibits the angiogenic potential of endometrial stromal cells under hypoxia by targeting HIF-1α/VEGF pathway.Int J Clin Exp Pathol,2015,8:4735-4744.
14 Celik O,Ersahin A,Acet M,et al.Disulfiram,as a candidate NF-κB and proteasome inhibitor,prevents endometriotic implant growing in a rat model of endometriosis.Eur Rev Med Pharmacol Sci,2016,20:4380-4389.
15 Chang Kai-Kai,Liu Li-Bing,Li Hui,et al.TSLP induced by estrogen stimulates secretion of MCP-1 and IL-8 and growth of human endometrial stromal cells through JNK and NF-κB signal pathways.Int J Clin Exp Pathol,2014,7:1889-1899.
16 Tao Xiang,Xie Yan,Wang Li,et al.The expression of Cox-2,NF-κB,and VEGF in ectopic endometrial tissues within fallopian tubes suggests different etiologies.Int J Gynecol Pathol,2014,33:411-417.
17 Yamanaka Kaoruko,Xu Bing,Suganuma Izumi,et al.Dienogest inhibits aromatase and cyclooxygenase-2 expression and prostaglandin E production in human endometriotic stromal cells in spheroid culture.Fertil Steril,2012,97:477-482.
18 Wang Xue-Rong,Hao Hong-Guang,Chu Lan.Glycyrrhizin inhibits LPS-induced inflammatory mediator production in endometrial epithelial cells.Microb Pathog,2017,109:110-113.
19 Takai Eri,Taniguchi Fuminori,Nakamura Kazuomi,et al.Parthenolide reduces cell proliferation and prostaglandin E2 [corrected] in human endometriotic stromal cells and inhibits development of endometriosis in the murine model.Fertil Steril,2013,100:1170-1178.
20 Song Tao,Zhang Xu,Yang Guoqiang,et al.Decrement of miR-199a-5p contributes to the tumorigenesis of bladder urothelial carcinoma by regulating MLK3/NF-κB pathway.Am J Transl Res,2015,7:2786-2794.
21 Jiang Yuan,Zhu Ying,Wang Xinxin,et al.Temporal regulation of HIF-1 and NF-κB in hypoxic hepatocarcinoma cells.Oncotarget,2015,6:9409-9419.
22 Bo Zhang,Xiang Qian,Shan-Ming Ruan,et al.Investigation on Molecular Mechanism of Fibroblast Regulation and the Treatment of Recurrent Oral Ulcer by Shuizhongcao Granule-Containing Serum.Evid Based Complement Alternat Med,2015,2015:324091.
23 Li Yue,Wang Xiaoyan,Wang Xishuang,et al.PDCD4 suppresses proliferation,migration,and invasion of endometrial cells by inhibiting autophagy and NF-κB/MMP2/MMP9 signal pathway.Biol Reprod,2018,99:360-372.
24 Esposito Evangelina,Aldrees Sultan,Mastromonaco Christina,et al.Evaluation of nicotinamide as an anti-inflammatory and anti-angiogenic agent in uveal melanoma cell lines.Arq Bras Oftalmol,2017,80:74-77.
25 Mulayim Naciye,Savlu Asim,Guzeloglu-Kayisli Ozlem,et al.Regulation of endometrial stromal cell matrix metalloproteinase activity and invasiveness by interleukin-8.Fertil Steril,2004,null:904-911.
26 Garcia-Velasco Juan A,Mulayim Naciye,Kayisli Umit A,et al.Elevated soluble Fas ligand levels may suggest a role for apoptosis in women with endometriosis.Fertil Steril,2002,78:855-859.
27 Sharpe-Timms KL,Nabli H,Zimmer RL,et al.Inflammatory cytokines differentially up-regulate human endometrial haptoglobin production in women with endometriosis.Hum Reprod ,2010,25:1241-1250.
28 Brenner Robert M,Nayak Nihar R,Slayden Ov D,et al.Premenstrual and menstrual changes in the macaque and human endometrium:relevance to endometriosis.Ann N Y Acad Sci,2002,955:60-74;discussion 86-88,396-406.
29 Luo Xue-zhen,Zhou Wen-jie,Tao Yu,et al.TLR4 Activation Promotes the Secretion of IL-8 Which Enhances the Invasion and Proliferation of Endometrial Stromal Cells in an Autocrine Manner via the FAK Signal Pathway.Am J Reprod Immunol ,2015,74:467-479.
30 Selam Belgin,Kayisli Umit A,Garcia-Velasco Juan A,et al.Regulation of fas ligand expression by IL-8 in human endometrium.J Clin Endocrinol Metab,2002,87:3921-3927.
31 Kim Ji-Yeon,Lee Dong-Hyung,Joo Jong-Kil,et al.Effects of peritoneal fluid from endometriosis patients on interferon-gamma-induced protein-10 (CXCL10)and interleukin-8 (CXCL8)released by neutrophils and CD4+ T cells.Am J Reprod Immunol,2009,62:128-138.
32 Cousins Fiona L,O Dorien F,Gargett Caroline E.Endometrial stem/progenitor cells and their role in the pathogenesis of endometriosis.Best Pract Res Clin Obstet Gynaecol,2018,50:27-38.
33 易秋红.MiRNA-199a、MMP-9在子宫内膜异位症患者中的表达及意义.山西医科大学,2016.
34 Izumi Gentaro,Koga Kaori,Takamura Masashi,et al.Involvement of immune cells in the pathogenesis of endometriosis.J Obstet Gynaecol Res,2018,44:191-198.
35 Wang Dan,Jiang YIling,Yang Xiaoxin,et al.6-Shogaol reduces progression of experimental endometriosis and regulation of VGEF and inhibition of COX-2 and PGE2-mediated inflammatory responses.Korean J Physiol Pharmacol,2018,22:627-636.
36 Marí-Alexandre Josep,Barceló-Molina Moisés,Belmonte-López Elisa,et al.Micro-RNA profILe and proteins in peritoneal fluid from women with endometriosis:their relationship with sterILity.FertIL SterIL,2018,109:675-684.e2.
37 Agrawal Swati,Tapmeier Thomas,Rahmioglu Nilufer,et al.The miRNA Mirage:How Close Are We to Finding a Non-Invasive Diagnostic Biomarker in Endometriosis A Systematic Review.Int J Mol Sci,2018,19:undefined.
38 Wu J,Cui SH,Li HZ,et al.Ultrasound diagnosis in gynecological acute abdomen.J Biol Regul Homeost Agents,2016,30:211-217.
39 Hsu Chia-Yi,Hsieh Tsung-Hua,Tsai Cheng-Fang,et al.Synthetic Steroid Hormones Regulated Cell Proliferation Through MicroRNA-34a-5p in Human Ovarian Endometrioma.Biol Reprod,2016,94:60.
40 Wang Wen-Tao,Zhao Ya-Nan,Han Bo-Wei,et al.Circulating microRNAs identified in a genome-wide serum microRNA expression analysis as noninvasive biomarkers for endometriosis.J Clin Endocrinol Metab,2013,98:281-289.
41 Young Vicky J,Ahmad Syed F,Brown Jeremy K,et al.Peritoneal VEGF-A expression is regulated by TGF-β1 through an ID1 pathway in women with endometriosis.Sci Rep,2015,5:16859.
42 Hall M,Gourley C,McNeish I,et al.Targeted anti-vascular therapies for ovarian cancer:current evidence.Br J Cancer,2013,108:250-258.
43 Moon Jeong Min,Chung Youn-Jee,Chae Boah,et al.Effect of mistletoe on endometrial stromal cell survival and vascular endothelial growth factor expression in patients with endometriosis.Int J Med Sci,2018,15:1530-1536.
44 Pourjafar M,Saidijam M,Mansouri K,et al.All-trans retinoic acid preconditioning enhances proliferation,angiogenesis and migration of mesenchymal stem cell in vitro and enhances wound repair in vivo.Cell Prolif,2017,50:undefined.
45 Yerlikaya Gülen,Balendran Sukirthini,Prstling Katharina,et al.Comprehensive study of angiogenic factors in women with endometriosis compared to women without endometriosis.Eur J Obstet Gynecol Reprod Biol,2016,204:88-98.
46 Dai Lan,Gu Li-ying,Zhu Jie,et al.Regulation of microRNA-199a on adhesion,migration and invasion ability of human endometrial stromal cells.Zhonghua Fu Chan Ke Za Zhi,2011,46:817-821.
47 Hsu Chia-Yi,Hsieh Tsung-Hua,Tsai Cheng-Fang,et al.miRNA-199a-5p regulates VEGFA in endometrial mesenchymal stem cells and contributes to the pathogenesis of endometriosis.J Pathol,2014,232:330-343.
48 Lin Shih-Chieh,Wang Chih-Chuan,Wu Meng-Hsing,et al.Hypoxia-induced microRNA-20a expression increases ERK phosphorylation and angiogenic gene expression in endometriotic stromal cells.J Clin Endocrinol Metab,2012,97:E1515-1523.
49 Hou Jingying,Wang Lingyun,Wu Quanhua,et al.Long noncoding RNA H19 upregulates vascular endothelial growth factor A to enhance mesenchymal stem cells survival and angiogenic capacity by inhibiting miR-199a-5p.Stem Cell Res Ther,2018,9:109.
50 Kim Ji-Hyun,Yang Yeong-In,Lee Kyung-Tae,et al.Costunolide induces apoptosis in human endometriotic cells through inhibition of the prosurvival Akt and nuclear factor kappa B signaling pathway.Biol Pharm Bull,2011,34:580-585.
51 Xing Yu,Li Ling.Gastrodin protects rat cardiomyocytes H9c2 from hypoxia-induced injury by up-regulation of microRNA-21.Int J Biochem Cell Biol,2019,109:8-16.
52 Hirata Yukihiro,Murai Noriyuki,Yanaihara Nozomu,et al.MicroRNA-21 is a candidate driver gene for 17q23-25 amplification in ovarian clear cell carcinoma.BMC Cancer,2014,14:799.
53 Lijie He,Shiren Sun,Hanmin Wang,et al.miRNA-199a/214 Cluster Enhances Fibrosis of Human Peritoneal Mesothelial Cells Regulated by SRF.Hong Kong Journal of Nephrology,2015,17:412-421.
54 Er Tze-Kiong,Su Yu-Fa,Wu Chun-Chieh,et al.Targeted next-generation sequencing for molecular diagnosis of endometriosis-associated ovarian cancer.J Mol Med,2016,94:835-847.
55 林勤,郑轩,杨华静,等.MiRNA-199a-3p通过mTOR通路与阿霉素联合抑制子宫内膜癌细胞的增殖和促凋亡作用.现代妇产科进展,2014,23:673-676.
56 Matsuzaki S,Canis M,Pouly JL,et al.Relationship between delay of surgical diagnosis and severity of disease in patients with symptomatic deep infILtrating endometriosis.FertIL SterIL,2006,86:1314-1316.
57 Wang Fang,Wang Hongxia,Jin Danting,et al.Serum miR-17,IL-4,and IL-6 levels for diagnosis of endometriosis.Medicine (Baltimore),2018,97:e10853.