摘要
利用AFLP技术对48份菌草种质资源进行了遗传多样性分析。首先,在海南象草等4份菌草材料上检测了20个AFLP引物组合的扩增情况。然后,从中筛选出6个扩增效果较好的组合用来进一步分析48份菌草种质材料的DNA,共产生了208个多态性条带。用NTSYS-pc 2.1软件结合UPGMA聚类分析方法对数据进行分析,结果显示,48份种质材料间遗传相似系数(SM)在0.48~0.98,在遗传相似系数为0.57时可将48份材料分成4个类群。以上研究结果表明,AFLP分子标记技术的分析结果能直观地表现菌草种质材料之间的亲缘关系,为菌草种质资源的收集和评价提供了一项新的技术手段。
In this study, AFLP technique was used to study the genetic diversity of 48 JUCAO germplasms. A total of 20 combinations of AFLP primer pairs were firstly tested on a subset of four samples. Then six combinations of AFLP primer pairs were selected and analyzed the DNA of 48 JUNCAO germplasms and a total of 208 polymorphic DNA fragments were obtained. The genetic similarity coefficient(SM) was estimated among all accessions by NTSYS-pc 2.1 software and ranged from 0.48 to 0.98. The 48 JUNCAO materials were separated into four clusters when the SM was 0.57.The results showed that the AFLP technique could more directly reveal the genetic difference among different accessions, and be served as a new technique for the collection and evaluation of JUNCAO germplasms.
引文
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