植物中多胺的合成代谢及其分子生物学水平研究进展
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摘要
多胺广泛存在于动植物体内,具有促进植物生长发育和延缓果实后熟进程等作用,但对其研究大多停留在生理水平,还需从分子生物学水平深入了解其调控机理以及代谢中各通路之间的相互作用关系。本文介绍了多胺生物合成与分解代谢过程,阐述了多胺及其代谢产物的生理功能,同时以国内外研究文献为基础,探讨植物中多胺代谢相关基因的分子生物学作用,指出了目前存在的问题,并为其研究方向提供了建议。
Polyamine widely exists in plants and animals, playing a role in promoting plant growth and development and delaying the process of fruit ripening. However, most of researches stay in the physiological level, it is also necessary to understand the mechanism of regulation and the interaction between the pathways in mechanism from the perspective of molecular biology. In this paper, the biosynthesis and catabolism processes of polyamine are introduced, the physiological functions of polyamines and their metabolites are elaborated, at the same time, based on domestic and foreign research literatures, the molecular biological functions of genes related to polyamine metabolism in plants are explored, the existing problems are pointed out and suggestions for the research direction are provided.
Polyamine widely exists in plants and animals, playing a role in promoting plant growth and development and delaying the process of fruit ripening. However, most of researches stay in the physiological level, it is also necessary to understand the mechanism of regulation and the interaction between the pathways in mechanism from the perspective of molecular biology. In this paper, the biosynthesis and catabolism processes of polyamine are introduced, the physiological functions of polyamines and their metabolites are elaborated, at the same time, based on domestic and foreign research literatures, the molecular biological functions of genes related to polyamine metabolism in plants are explored, the existing problems are pointed out and suggestions for the research direction are provided.
引文
[1]马瑛,刘静.植物体中多胺代谢及其功能研究进展[J].陕西理工学院学报(自然科学版),2010,26(2):47-54.DOI:10.3969/j.issn.1673-2944.2010.02.013.
[2]NAMBEESAN S,ABUQAMAR S,LALUK K,et al.Polyamines attenuate ethylene-mediated defense responses to abrogate resistance to Botrytis cinerea in tomato[J].Plant Physiology,2012,158(2):1034-1045.DOI:10.1104/pp.111.188698.
[3]MATTOO A K,HANDA A K.Higher polyamines restore and enhance metabolic memory in ripening fruit[J].Plant Science,2008,174(4):386-393.DOI:10.1016/j.plantsci.2008.01.011.
[4]TORRIGIANI P,BREGOLI A M,ZIOSI V,et al.Molecular and biochemical aspects underlying polyamine modulation of fruit development and ripening[J].Stewart Postharvest Review,2008,4(2):1-12.DOI:10.2212/spr.2008.2.8.
[5]刘颖,王莹,龙萃,等.植物多胺代谢途径研究进展[J].生物工程学报,2011,27(2):147-155.DOI:10.13345/j.cjb.2011.02.002.
[6]田婧.外源亚精胺缓解黄瓜幼苗高温胁迫伤害的生理调节机制和蛋白质组学研究[D].南京:南京农业大学,2012.DOI:10.7666/d.Y2359878.
[7]杜红阳.干旱胁迫下小麦发育籽粒胚细胞内结合态多胺的功能[D].郑州:河南农业大学,2015.
[8]陈俊琴.辣椒果实中辣椒素生物合成相关物质及外源多胺调控的研究[D].沈阳:沈阳农业大学,2015.
[9]许红.干旱胁迫下多胺对小麦种子萌发的影响及其机理研究[D].杨凌:西北农林科技大学,2016.
[10]吕晓康,温晓霞,廖允成,等.外源多胺对小麦小花退化的调控机制[J].作物学报,2016,42(9):1391-1401.DOI:10.3724/SP.J.1006.2016.01391.
[11]周江,张辉,吴斌,等.亚精胺处理对无核白葡萄贮藏品质的影响[J].保鲜与加工,2015,15(4):17-20.DOI:10.3969/j.issn.1009-6221.2015.04.004.
[12]应叶青.多胺对毛竹幼苗抗旱调控机理的研究[D].北京:北京林业大学,2013.
[13]张毅.亚精胺对番茄幼苗盐碱胁迫的缓解效应及其调控机理[D].杨凌:西北农林科技大学,2013.
[14]张同祯.多胺氧化酶在光抑制玉米中胚轴伸长中的作用[D].兰州:甘肃农业大学,2016.
[15]AZIZ A,MARTIN-TANGUY J,LARHER F.Plasticity of polyamine metabolism associated with high osmotic stress in rape leaf discs and with ethylene treatment[J].Plant Growth Regulation,1997,21(2):153-163.
[16]KAUR-SAWHNEY R,GALSTON A W.Physiological and biochemical studies on the antisenescence properties of polyamines in plants[M]//Biochemistry and Physiology of Polyamines in Plants.Boca Raton:CRC Press,1991:201-211.
[17]杨泽伟,王龙海,朱莉,等.γ-氨基丁酸代谢旁路在植物响应逆境胁迫中的作用机制研究[J].生物技术进展,2014,4(2):77-84.DOI:10.3969/j.issn.2095-2341.2014.02.01.
[18]郭元新.盐和低氧胁迫下发芽大豆γ-氨基丁酸富集与调控机理研究[D].南京:南京农业大学,2011.DOI:10.7666/d.Y2360390.
[19]MIRABELLA R,RAUWERDA H,STRUYS E A,et al.The Arabidopsis her1 mutant implicates GABA in E-2-hexenal responsiveness[J].The Plant Journal:for Cell and Molecular Biology,2008,53(2):197-213.DOI:10.1111/j.1365-313X.2007.03323.x.
[20]ROSSI F R,MARINA M,PIECKENSTAIN F L.Role of Arginine decarboxylase(ADC)in Arabidopsis thaliana defence against the pathogenic bacterium Pseudomonas viridiflava[J].Plant Biology,2015,17(4):831-839.DOI:10.1111/plb.12289.
[21]LI Y D,MO H J,WANG X F,et al.Cloning and expression analysis of an arginine decarboxylase gene from Gossypium hirsutum[J].Cotton Science,2013,25(4):291-299.
[22]孙培培.枳精氨酸脱羧酶基因PtADC抗逆功能解析及其调控因子分离与鉴定[D].武汉:华中农业大学,2014.DOI:10.7666/d.Y2565968.
[23]乔梦吉.柽柳离体培养体系的建立及耐盐性研究[D].北京:北京林业大学,2007.
[24]秦白富.颠茄ODC基因克隆和功能分析[D].重庆:西南大学,2014.
[25]DALTON H L,BLOMSTEDT C K,NEALE A D,et al.Effects of down-regulating ornithine decarboxylase upon putrescineassociated metabolism and growth in Nicotiana tabacum L.[J].Journal of Experimental Botany,2016,67(11):3367-3381.DOI:10.1093/jxb/erw166.
[26]HANFREY C,ELLIOTT K A,FRANCESCHETTI M,et al.Adual upstream open reading frame-based autoregulatory circuit controlling polyamine-responsive translation[J].Journal of Biological Chemistry,2005,280(47):39229-39237.DOI:10.1074/jbc.M509340200.
[27]文乐.甘蔗S-腺苷蛋氨酸脱羧酶基因(ScSAMDC)的克隆和转化烟草的研究[D].南宁:广西大学,2015.
[28]张梅,王然,马春晖,等.杜梨S-腺苷甲硫氨酸脱羧酶基因的克隆与生物信息学分析[J].华北农学报,2013,28(1):82-87.DOI:10.3969/j.issn.1000-7091.2013.01.016.
[29]WEN X P,BAN Y,INOUE H,et al.Aluminum tolerance in a spermidine synthase-overexpressing transgenic European pear is correlated with the enhanced level of spermidine via alleviating oxidative status[J].Environmental and Experimental Botany,2009,66(3):471-478.DOI:10.1016/j.envexpbot.2009.03.014.
[30]向玥茹.植物亚精胺合成酶基因(MdSPDS)导入马铃薯的研究[D].长沙:湖南农业大学,2013.
[31]GUO H N,XIA Z H,LI D J.Molecular cloning,expression patterns and characterization of a new spermidine synthase gene in Oryza sativa[J].Chinese Journal of Tropical Crops,2013,34:285-291.
[32]王伟.甜橙多胺氧化酶基因克隆及功能鉴定[D].武汉:华中农业大学,2016.
[33]ONO Y,KIM D W,WATANABE K,et al.Constitutively and highly expressed Oryza sativa,polyamine oxidases localize in peroxisomes and catalyze polyamine back conversion[J].Amino Acids,2012,42(2-3):867-876.DOI:10.1007/s00726-011-1002-3.
[34]MO H J,WANG X F,ZHANG Y,et al.Cotton polyamine oxidase is required for spermine and camalexin signalling in the defence response to Verticillium dahliae[J].The Plant Journal:for Cell and Molecular Biology,2015,83(6):962-975.DOI:10.1111/tpj.12941.
[35]安振锋.二胺氧化酶在ABA调节过氧化氢产生和蚕豆气孔运动中的作用及其机理[D].南京:南京农业大学,2008.DOI:10.7666/d.Y1491995.
[36]徐港明.大豆下胚轴诱导不定根再生过程中二胺氧化酶基因表达及酶活性变化[D].苏州:苏州大学,2016.
[2]NAMBEESAN S,ABUQAMAR S,LALUK K,et al.Polyamines attenuate ethylene-mediated defense responses to abrogate resistance to Botrytis cinerea in tomato[J].Plant Physiology,2012,158(2):1034-1045.DOI:10.1104/pp.111.188698.
[3]MATTOO A K,HANDA A K.Higher polyamines restore and enhance metabolic memory in ripening fruit[J].Plant Science,2008,174(4):386-393.DOI:10.1016/j.plantsci.2008.01.011.
[4]TORRIGIANI P,BREGOLI A M,ZIOSI V,et al.Molecular and biochemical aspects underlying polyamine modulation of fruit development and ripening[J].Stewart Postharvest Review,2008,4(2):1-12.DOI:10.2212/spr.2008.2.8.
[5]刘颖,王莹,龙萃,等.植物多胺代谢途径研究进展[J].生物工程学报,2011,27(2):147-155.DOI:10.13345/j.cjb.2011.02.002.
[6]田婧.外源亚精胺缓解黄瓜幼苗高温胁迫伤害的生理调节机制和蛋白质组学研究[D].南京:南京农业大学,2012.DOI:10.7666/d.Y2359878.
[7]杜红阳.干旱胁迫下小麦发育籽粒胚细胞内结合态多胺的功能[D].郑州:河南农业大学,2015.
[8]陈俊琴.辣椒果实中辣椒素生物合成相关物质及外源多胺调控的研究[D].沈阳:沈阳农业大学,2015.
[9]许红.干旱胁迫下多胺对小麦种子萌发的影响及其机理研究[D].杨凌:西北农林科技大学,2016.
[10]吕晓康,温晓霞,廖允成,等.外源多胺对小麦小花退化的调控机制[J].作物学报,2016,42(9):1391-1401.DOI:10.3724/SP.J.1006.2016.01391.
[11]周江,张辉,吴斌,等.亚精胺处理对无核白葡萄贮藏品质的影响[J].保鲜与加工,2015,15(4):17-20.DOI:10.3969/j.issn.1009-6221.2015.04.004.
[12]应叶青.多胺对毛竹幼苗抗旱调控机理的研究[D].北京:北京林业大学,2013.
[13]张毅.亚精胺对番茄幼苗盐碱胁迫的缓解效应及其调控机理[D].杨凌:西北农林科技大学,2013.
[14]张同祯.多胺氧化酶在光抑制玉米中胚轴伸长中的作用[D].兰州:甘肃农业大学,2016.
[15]AZIZ A,MARTIN-TANGUY J,LARHER F.Plasticity of polyamine metabolism associated with high osmotic stress in rape leaf discs and with ethylene treatment[J].Plant Growth Regulation,1997,21(2):153-163.
[16]KAUR-SAWHNEY R,GALSTON A W.Physiological and biochemical studies on the antisenescence properties of polyamines in plants[M]//Biochemistry and Physiology of Polyamines in Plants.Boca Raton:CRC Press,1991:201-211.
[17]杨泽伟,王龙海,朱莉,等.γ-氨基丁酸代谢旁路在植物响应逆境胁迫中的作用机制研究[J].生物技术进展,2014,4(2):77-84.DOI:10.3969/j.issn.2095-2341.2014.02.01.
[18]郭元新.盐和低氧胁迫下发芽大豆γ-氨基丁酸富集与调控机理研究[D].南京:南京农业大学,2011.DOI:10.7666/d.Y2360390.
[19]MIRABELLA R,RAUWERDA H,STRUYS E A,et al.The Arabidopsis her1 mutant implicates GABA in E-2-hexenal responsiveness[J].The Plant Journal:for Cell and Molecular Biology,2008,53(2):197-213.DOI:10.1111/j.1365-313X.2007.03323.x.
[20]ROSSI F R,MARINA M,PIECKENSTAIN F L.Role of Arginine decarboxylase(ADC)in Arabidopsis thaliana defence against the pathogenic bacterium Pseudomonas viridiflava[J].Plant Biology,2015,17(4):831-839.DOI:10.1111/plb.12289.
[21]LI Y D,MO H J,WANG X F,et al.Cloning and expression analysis of an arginine decarboxylase gene from Gossypium hirsutum[J].Cotton Science,2013,25(4):291-299.
[22]孙培培.枳精氨酸脱羧酶基因PtADC抗逆功能解析及其调控因子分离与鉴定[D].武汉:华中农业大学,2014.DOI:10.7666/d.Y2565968.
[23]乔梦吉.柽柳离体培养体系的建立及耐盐性研究[D].北京:北京林业大学,2007.
[24]秦白富.颠茄ODC基因克隆和功能分析[D].重庆:西南大学,2014.
[25]DALTON H L,BLOMSTEDT C K,NEALE A D,et al.Effects of down-regulating ornithine decarboxylase upon putrescineassociated metabolism and growth in Nicotiana tabacum L.[J].Journal of Experimental Botany,2016,67(11):3367-3381.DOI:10.1093/jxb/erw166.
[26]HANFREY C,ELLIOTT K A,FRANCESCHETTI M,et al.Adual upstream open reading frame-based autoregulatory circuit controlling polyamine-responsive translation[J].Journal of Biological Chemistry,2005,280(47):39229-39237.DOI:10.1074/jbc.M509340200.
[27]文乐.甘蔗S-腺苷蛋氨酸脱羧酶基因(ScSAMDC)的克隆和转化烟草的研究[D].南宁:广西大学,2015.
[28]张梅,王然,马春晖,等.杜梨S-腺苷甲硫氨酸脱羧酶基因的克隆与生物信息学分析[J].华北农学报,2013,28(1):82-87.DOI:10.3969/j.issn.1000-7091.2013.01.016.
[29]WEN X P,BAN Y,INOUE H,et al.Aluminum tolerance in a spermidine synthase-overexpressing transgenic European pear is correlated with the enhanced level of spermidine via alleviating oxidative status[J].Environmental and Experimental Botany,2009,66(3):471-478.DOI:10.1016/j.envexpbot.2009.03.014.
[30]向玥茹.植物亚精胺合成酶基因(MdSPDS)导入马铃薯的研究[D].长沙:湖南农业大学,2013.
[31]GUO H N,XIA Z H,LI D J.Molecular cloning,expression patterns and characterization of a new spermidine synthase gene in Oryza sativa[J].Chinese Journal of Tropical Crops,2013,34:285-291.
[32]王伟.甜橙多胺氧化酶基因克隆及功能鉴定[D].武汉:华中农业大学,2016.
[33]ONO Y,KIM D W,WATANABE K,et al.Constitutively and highly expressed Oryza sativa,polyamine oxidases localize in peroxisomes and catalyze polyamine back conversion[J].Amino Acids,2012,42(2-3):867-876.DOI:10.1007/s00726-011-1002-3.
[34]MO H J,WANG X F,ZHANG Y,et al.Cotton polyamine oxidase is required for spermine and camalexin signalling in the defence response to Verticillium dahliae[J].The Plant Journal:for Cell and Molecular Biology,2015,83(6):962-975.DOI:10.1111/tpj.12941.
[35]安振锋.二胺氧化酶在ABA调节过氧化氢产生和蚕豆气孔运动中的作用及其机理[D].南京:南京农业大学,2008.DOI:10.7666/d.Y1491995.
[36]徐港明.大豆下胚轴诱导不定根再生过程中二胺氧化酶基因表达及酶活性变化[D].苏州:苏州大学,2016.