86份酸枣种质资源筛选和遗传多样性分析
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摘要
[目的]综合酸枣种质资源主要农艺性状评价和遗传多样性分析结果,筛选优良酸枣种质资源。[方法]对86份酸枣种质资源的15个农艺性状进行表型鉴定,并利用9个ISSR标记进行遗传多样性分析。[结果]通过对15个农艺性状综合评价,从86份酸枣种质资源中筛选出5份优良种质资源。9对ISSR引物共扩增出20条多态性条带,多态性信息量(polymorphism information content,PIC)为0.60～0.81,平均值为0.70。在遗传系数0.57的水平上86份酸枣种质资源被分为两大类,其中79份种质资源聚为A类,7份种质资源聚为B类。[结论]该研究为酸枣种质资源分类、鉴定和新品种选育等提供理论依据。
[Objective] The main agronomic traits and genetic diversity analysis results were studied to screen the excellent sour jujube germplasm resources.[Method] 15 agronomic traits of 86 sour jujube germplasm resources were identified,and 9 inter-simple sequence repeat(ISSR) primers were used for genetic diversity analysis.[Result] Through the comprehensive evaluation of 15 agronomic traits,5 excellent germplasm resources were selected from 86 sour jujube individuals.A total of 20 polymorphic bands were amplified by 9 ISSR primers.The polymorphism information content(PIC) ranged from 0.60 to 0.81 with an average of 0.70.Clustering analysis showed that the 86 sour jujube germplasm resources could be divided into 2 groups at the genetic similarity coefficient of 0.57,79 sour jujube individuals were grouped into class A and 7 sour jujube individuals were grouped into class B.[Conclusion] This study provides a useful basis for the classification,identification and variety selection of sour jujube germplasm resources.
[Objective] The main agronomic traits and genetic diversity analysis results were studied to screen the excellent sour jujube germplasm resources.[Method] 15 agronomic traits of 86 sour jujube germplasm resources were identified,and 9 inter-simple sequence repeat(ISSR) primers were used for genetic diversity analysis.[Result] Through the comprehensive evaluation of 15 agronomic traits,5 excellent germplasm resources were selected from 86 sour jujube individuals.A total of 20 polymorphic bands were amplified by 9 ISSR primers.The polymorphism information content(PIC) ranged from 0.60 to 0.81 with an average of 0.70.Clustering analysis showed that the 86 sour jujube germplasm resources could be divided into 2 groups at the genetic similarity coefficient of 0.57,79 sour jujube individuals were grouped into class A and 7 sour jujube individuals were grouped into class B.[Conclusion] This study provides a useful basis for the classification,identification and variety selection of sour jujube germplasm resources.
引文
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[25] 武媛林.酸枣种质资源的评价及选优[D].保定:河北农业大学,2008.
[26] 王僧虎,石晓云.酸枣优良新品种邢州1号的选育[J].湖北农业科学,2012,51(20):4545-4548.
[27] 王春桐.酸枣极早熟新品种脆酸枣的选育[J].中国果业信息,2008(1):55.
[28] 张建英,毛向红,张莹莹,等.酸枣新品种‘蓝猫1号’和‘蓝猫2号’的选育[J].果树学报,2017,34(6):775-777.
[2] 张春梅.酸枣SSR引物开发及遗传多样性研究[D].杨凌:西北农林科技大学,2013.
[3] 刘孟军,汪民.中国枣种质资源[M].北京:中国林业出版社,2009.
[4] ISLAM M B,SIMMONS M P.A thorny dilemma:Testing alternative intrageneric classifications within Ziziphus (Rhamnaceae)[J].Systematic botany,2006,31(4):826-842.
[5] ZHANG C M,HUANG J,YIN X,et al.Genetic diversity and population structure of sour jujube,Ziziphus acidojujuba[J].Tree genetics & genomes,2015,11(1):809.
[6] 南京中医药大学.中药大辞典:下册[S].2版.上海:上海科学技术出版社,2006:3577-3579.
[7] 张雪,丁长河,李和平.酸枣仁的化学成分和药理作用研究进展[J].食品工业科技,2009,30(3):348-350.
[8] 张明春,解军波,李婷,等.复合酶法提取酸枣仁黄酮研究[J].食品科学,2008,29(9):174-177.
[9] 王卫记,罗建光,孔令义.HPLC-ESI-MSn分析酸枣仁有效部位的化学成分[J].中国中药杂志,2009,34(21):2768-2772.
[10] 赵连红,乔卫,许岚.酸枣仁中生物碱抗惊厥作用的实验研究[J].天津药学,2007,19(1):4-5.
[11] 耿欣,李廷利.酸枣仁主要化学成分及药理作用研究进展[J].中医药学报,2016,44(5):84-86.
[12] 闫艳,杜晨晖,李小菊,等.HPLC-DAD-ELSD法同时测定酸枣仁中斯皮诺素、酸枣仁皂苷A和B的含量[J].药物分析杂志,2011,31(1):30-33.
[13] 杨雷.酸枣种质资源果实营养成份分析及种质评价[D].保定:河北农业大学,2004.
[14] 孙亚强,吴翠云,王德,等.酸枣种质资源果实若干性状的多元统计分析[J].江西农业学报,2015,27(12):29-32.
[15] 张春梅.酸枣SSR引物开发及遗传多样性研究[D].杨凌:西北农林科技大学,2013.
[16] 张鹏飞,尉东峰,刘亚令,等.枣与酸枣的SSR遗传多样性研究[J].华北农学报,2015,30(2):150-155.
[17] ZIETKIEWICZ E,RAFALSKI A,LABUDA D.Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification[J].Genomics,1994,20(2):176-183.
[18] REDDY M P,SARLA N,SIDDIQ E A.Inter simple sequence repeat (ISSR) polymorphism and its application in plant breeding[J].Euphytica,2002,128(1):9-17.
[19] LIU M J,ZHAO J,CAI Q L,et al.The complex jujube genome provides insights into fruit tree biology[J].Nature communications,2014,5:1-14.
[20] 李登科.枣种质资源描述规范和数据标准[M].北京:中国农业出版社,2006.
[21] LIAN C L,WADUD M A,GENG Q F,et al.An improved technique for isolating codominant compound microsatellite markers[J].Journal of plant research,2006,119(4):415-417.
[22] ROHLF F J.NTSYS-pc:Numerical taxonomy system.Ver.2.1[M].New York:Applied Biostatistics Inc.,1998.
[23] BOTSTEIN D,WHITE R L,SKOLNICK M,et al.Construction of a genetic linkage map in man using restriction fragment length polymorphisms[J].American journal of human genetics,1980,32(3):314-331.
[24] 曹景权,许向一.野生酸枣的类型与利用[J].河北果树,2011(1):49-50.
[25] 武媛林.酸枣种质资源的评价及选优[D].保定:河北农业大学,2008.
[26] 王僧虎,石晓云.酸枣优良新品种邢州1号的选育[J].湖北农业科学,2012,51(20):4545-4548.
[27] 王春桐.酸枣极早熟新品种脆酸枣的选育[J].中国果业信息,2008(1):55.
[28] 张建英,毛向红,张莹莹,等.酸枣新品种‘蓝猫1号’和‘蓝猫2号’的选育[J].果树学报,2017,34(6):775-777.