Electroformation and collection of giant liposomes on an integrated microchip

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英文篇名：Electroformation and collection of giant liposomes on an integrated microchip 作者：Zhenyu ; Wang ; Chaoxiong ; Wu ; Ting ; Fan ; Xianwei ; Han ; Qiong ; Wang ; Jincan ; Lei ; Jun ; Yang 英文作者：Zhenyu Wang;Chaoxiong Wu;Ting Fan;Xianwei Han;Qiong Wang;Jincan Lei;Jun Yang;State Key Laboratory of Ultrasound Engineering in Medicine Co-Founded by Chongqing ξ the Ministry of Science ξ Technology, College of Biomedical Engineering, Chongqing Medical University;Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, and Key Lab of Visual Damage and Regeneration ξ Restoration of Chongqing,Bioengineering College, Chongqing University;Chongqing Engineering and Technology Research Center of Intelligent Rehabilitation and Eldercare, Chongqing City Management College; 英文关键词：Giant liposome;;Microchip;;ITO;;Electroformation;;Collection 中文刊名：FXKB 英文刊名：中国化学快报(英文版) 机构：State Key Laboratory of Ultrasound Engineering in Medicine Co-Founded by Chongqing ξ the Ministry of Science ξ Technology, College of Biomedical Engineering, Chongqing Medical University;Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, and Key Lab of Visual Damage and Regeneration ξ Restoration of Chongqing,Bioengineering College, Chongqing University;Chongqing Engineering and Technology Research Center of Intelligent Rehabilitation and Eldercare, Chongqing City Management College; 出版日期：2019-02-15 出版单位：Chinese Chemical Letters 年：2019 期：v.30 基金：supported by the National Natural Science Foundation of China(Nos. 81501617,81871450,21827812);; Science and Technology Planning Project of Yuzhong District, Chongqing, China(No. 20170122);; the Program of International S&T Cooperation (No. 2014DFG31380);; the Foundation for Higher Education Young Key Teacher of Chongqing, China 语种：英文; 页：FXKB201902016 页数：6 CN：02 ISSN：11-2710/O6 分类号：103-108

摘要

Giant liposome is an important lipid structure widely used in biological and medical fields. In its main preparation method, electroformation, many influencing factors must be optimized for good effect. How to collect the desired giant liposomes is another major issue. In this work, a microchip with a reactor chamber array was used to study the influences of multiple parameters, and a suitable condition could be achieved rapidly and efficiently. A tailor-made collection chamber was also integrated on the chip. Based on the multifactor and multilevel orthogonal experiment, optimal conditions of the lipid solution, buffer solution, and electric signal were achieved with high efficiency. More than one thousand giant liposomes could be formed in each microscale reactor chamber, and most of them were unilamellar. The on-chip collection ratio of giant liposome carriers could also approximate to 40%.
        Giant liposome is an important lipid structure widely used in biological and medical fields. In its main preparation method, electroformation, many influencing factors must be optimized for good effect. How to collect the desired giant liposomes is another major issue. In this work, a microchip with a reactor chamber array was used to study the influences of multiple parameters, and a suitable condition could be achieved rapidly and efficiently. A tailor-made collection chamber was also integrated on the chip. Based on the multifactor and multilevel orthogonal experiment, optimal conditions of the lipid solution, buffer solution, and electric signal were achieved with high efficiency. More than one thousand giant liposomes could be formed in each microscale reactor chamber, and most of them were unilamellar. The on-chip collection ratio of giant liposome carriers could also approximate to 40%.

引文

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