初始pH值对菌株UR-2诱导Cd(Ⅱ)碳酸盐成矿的影响
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摘要
碳酸盐矿化微生物通过可诱导碳酸盐成矿来固化重金属Cd~(2+)。为了探究pH条件对此过程的影响,选取碳酸盐矿化菌株UR-2,制备了不同初始pH条件(pH 5.0～10.0)下该菌株诱导Cd~(2+)成矿的产物。结果表明,菌株生长的最佳初始pH值为8.0左右,对0.2g/L Cd~(2+)的固化率达到70.5%。X射线衍射(XRD)、扫描电镜(SEM)分析结果显示,产物为碳酸镉(CdCO_3)。pH值的变化对菌株Cd~(2+)的络合能力以及矿化产物的形貌、组成均有较大影响。
Carbonate mineralized microorganisms solidify heavy metal Cd~(2+)ions by inducing carbonate mineralization.In order to explore the effect of pH conditions on this process,the carbonate mineralization strain UR-2was selected to produce the product of Cd~(2+)ion-induced mineralization under different initial pH conditions(pH 5.0-10.0).The results show that the optimum initial pH value for strain growth is about 8.0,and the solidification rate for 0.2g/L Cd~(2+)ion is 70.5%.X-ray diffraction(XRD)and scanning electron microscopy(SEM)analysis show that the product is cadmium carbonate(CdCO_3);with the change of pH value,the strain has great influence on the complexation ability of Cd~(2+)and the morphology and composition of mineralized products.
Carbonate mineralized microorganisms solidify heavy metal Cd~(2+)ions by inducing carbonate mineralization.In order to explore the effect of pH conditions on this process,the carbonate mineralization strain UR-2was selected to produce the product of Cd~(2+)ion-induced mineralization under different initial pH conditions(pH 5.0-10.0).The results show that the optimum initial pH value for strain growth is about 8.0,and the solidification rate for 0.2g/L Cd~(2+)ion is 70.5%.X-ray diffraction(XRD)and scanning electron microscopy(SEM)analysis show that the product is cadmium carbonate(CdCO_3);with the change of pH value,the strain has great influence on the complexation ability of Cd~(2+)and the morphology and composition of mineralized products.
引文
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[5]王艳娜.土壤重金属污染现状与防治方法[J].价值工程,2014(35):311-312.DOI:10.14018/j.cnki.cn13-1085/n.2014.35.178.WANG Y N.Pollution status and prevention methods of the soil heavy mental[J].Value Engineering,2014(35):311-312.DOI:10.14018/j.cnki.cn13-1085/n.2014.35.178(Ch).
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[7]李萌,郭红仙,程晓辉.土壤中产脲酶微生物分离及对重金属的固化[J].湖北农业科学,2013(14):3280-3282.DOI:10.14088/j.cnki.issn0439-8114.2013.14.016.LI M,GUO H X,CHENG X H.Isolation of urease producing bacteria from soil and its mineralization on heavy metal[J].Hubei Agricultural Sciences,2013(14):3280-3282.DOI:10.14088/j.cnki.issn0439-8114.2013.14.016(Ch).
[8]许燕波,钱春香,陆兆文.微生物矿化修复重金属污染土壤[J].环境工程学报,2013,7(7):2763-2768.XU Y B,QIAN C X,LU Z W.Remediation of heavy metal contaminated soils by bacteria biomineralization[J].Chinese Journal of Environmental Engineering,2013,7(7):2763-2768(Ch).
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[16]高连斌,张浩然,梁玉珍.镉-碘化钾-罗丹明B-三元络合物光度法测定镉[J].理化检验(化学分册),2006,42(9):719-720.DOI:10.3321/j.issn:1001-4020.2006.09.009.GAO L B,ZHANG H R,LIANG Y Z.Photometric determination of cadmium(Ⅱ)by the formation of ternary complex with potassium iodide and Rhodamine B[J].Physical Testing and Chemical Analysis Part B:Chemical Analysis,2006,42(9):719-720.DOI:10.3321/j.issn:1001-4020.2006.09.009(Ch).
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[18]刘爱民,黄为一.应用红外方法探讨耐镉菌株高积累Cd2+的机理[J].环境科学学报,2005,25(11):1502-1506.DOI:10.3321/j.issn:0253-2468.2005.11.012.LIU A M,HUANG W Y.Research on high accumulating Cd2+mechanism of a toletant-Cd2+strain by infrared spectroscopy analysis[J].Acta Scientiae Circumstantiae,2005,25(11):1502-1506.DOI:10.3321/j.issn:0253-2468.2005.11.012(Ch).