海藻酸钠降解菌的筛选及其发酵条件的优化
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摘要
从大连附近海域腐烂的海带中筛选得到一株能够降解海藻酸钠的菌株,经16S rDNA序列测定,该菌属于假交替单胞菌属(Pseudoalteromonas)。通过正交实验对其发酵条件进行优化,结果表明,该菌株的最佳产酶条件及培养基组成为(w/v):海藻酸钠0.7%,蛋白胨0.3%,NaCl2.5%,MgSO4·7H2O 0.025%,KH2PO4 0.5%,NaNO3 0.025%,FeSO4·7H2O 0.001%,pH7.2,接种量4%,转速180 r/min。条件优化后菌株酶活力达到51.498 U/mL,比优化前提高了11.5倍。
A strain degrading sodium alginate was isolated from the decayed sea weeds(Laminaria Japonica Aresch) in the sea area of Dalian, and it proved it belonged to the genus of Pseudoalteromonas by sequencing of 16 S rDNA. Then optimization of fermentation conditions were performed based on orthogonal experiments. The optimized condition was as followed(w/v): sodium alginate 0.7%, peptone 0.3%, NaCl 2.5%, MgSO4?7H2O 0.025%, KH2PO4 0.5%, NaNO3 0.025%, FeSO4?7H2O 0.001%, pH at 7.2, with 4% inoculation and a rotation rate of 180 r/min. The activity of sodium alginate lyase under this condition reached 51.498 U/mL, which was 11.5 times higher than that before optimization.
A strain degrading sodium alginate was isolated from the decayed sea weeds(Laminaria Japonica Aresch) in the sea area of Dalian, and it proved it belonged to the genus of Pseudoalteromonas by sequencing of 16 S rDNA. Then optimization of fermentation conditions were performed based on orthogonal experiments. The optimized condition was as followed(w/v): sodium alginate 0.7%, peptone 0.3%, NaCl 2.5%, MgSO4?7H2O 0.025%, KH2PO4 0.5%, NaNO3 0.025%, FeSO4?7H2O 0.001%, pH at 7.2, with 4% inoculation and a rotation rate of 180 r/min. The activity of sodium alginate lyase under this condition reached 51.498 U/mL, which was 11.5 times higher than that before optimization.
引文
[1]陈蕾,罗志刚,等.海藻酸钠在医学工程上的应用研究进展[J].医疗卫生装备,2008,29(9)
[2]Alexander D Augest,Hyun Joon Kong,David Mooney.Alginate Hydrogels as Biomaterials[J].Macromol Biosci,2006,6:623-633
[3]熊海燕,叶汉英.褐藻酸钠的生产及应用[J].食品研究与开发,2008,29(2)
[4]Yoshiko Iwamoto,Xu Xu,Tadashi Tamura,et al.Enzymatically depolymerized alginate oligomers that cause cytotoxic cytodine production in human monouclear cells[J].Biosci Biotech Siochem,2003,67(2):258-263
[5]Lin Chang-Zheng,Guan Hua-Shi,Li Hai-Hua,et al.The influence of molecular mass of sulfated propylene glycol ester of low-molecular-weight alginate on anticoagulant activities[J].European Polymer Journal,2007,43:3009-3015
[6]Mami Iwamotoa,Maki Kurachi,et al.Structure-activity relationship of alginate oligosaccharides in the induction of cytokine production from RAW264.7 cells[J].FEBS Letters,2005,579:4423-4429
[7]Le QuangLuan,Naotsugu Nagasawa,Vo Thi Thu Ha,et al.Enhancement of plant growth stimulation activity of iradiated alginate bt fractionation[J].Radiation Physics and Chemistry,2009,78:796-799
[8]傅晓妍,李京宝,韩峰,等.褐藻胶裂解酶产生菌Vibrosp QYl02的发酵条件优化[J].中国海洋大学学报,2007,37(3):432-436
[9]杨钊,范莹.不同降解方法制备褐藻胶甘露糖醛酸寡糖的结构特点[J].中国生化药物杂志,2007,28(1):69-71
[10]Kim DE,Lee EY,Kim HS.Cloning and characterization of alginate lyase from a marine bacterium Streptomyces sp.ALG-5[J].Mar Biotechnol,2009,11(1):10-16
[11]中山大学生物系生化微生物教研室.生化技术导论[M].北京:人民教育出版社,1978:61-62
[2]Alexander D Augest,Hyun Joon Kong,David Mooney.Alginate Hydrogels as Biomaterials[J].Macromol Biosci,2006,6:623-633
[3]熊海燕,叶汉英.褐藻酸钠的生产及应用[J].食品研究与开发,2008,29(2)
[4]Yoshiko Iwamoto,Xu Xu,Tadashi Tamura,et al.Enzymatically depolymerized alginate oligomers that cause cytotoxic cytodine production in human monouclear cells[J].Biosci Biotech Siochem,2003,67(2):258-263
[5]Lin Chang-Zheng,Guan Hua-Shi,Li Hai-Hua,et al.The influence of molecular mass of sulfated propylene glycol ester of low-molecular-weight alginate on anticoagulant activities[J].European Polymer Journal,2007,43:3009-3015
[6]Mami Iwamotoa,Maki Kurachi,et al.Structure-activity relationship of alginate oligosaccharides in the induction of cytokine production from RAW264.7 cells[J].FEBS Letters,2005,579:4423-4429
[7]Le QuangLuan,Naotsugu Nagasawa,Vo Thi Thu Ha,et al.Enhancement of plant growth stimulation activity of iradiated alginate bt fractionation[J].Radiation Physics and Chemistry,2009,78:796-799
[8]傅晓妍,李京宝,韩峰,等.褐藻胶裂解酶产生菌Vibrosp QYl02的发酵条件优化[J].中国海洋大学学报,2007,37(3):432-436
[9]杨钊,范莹.不同降解方法制备褐藻胶甘露糖醛酸寡糖的结构特点[J].中国生化药物杂志,2007,28(1):69-71
[10]Kim DE,Lee EY,Kim HS.Cloning and characterization of alginate lyase from a marine bacterium Streptomyces sp.ALG-5[J].Mar Biotechnol,2009,11(1):10-16
[11]中山大学生物系生化微生物教研室.生化技术导论[M].北京:人民教育出版社,1978:61-62