新疆维吾尔族老年人群延迟整流型钾离子通道KCNE1(G38S)基因多态性与心房颤动的相关性研究
|
摘要
目的探讨新疆维吾尔族老年人群延迟整流型钾离子通道KCNE1(G38S)基因多态性与心房颤动(AF)的相关性。方法收集新疆地区维吾尔族AF人群(AF组)和非AF人群(对照组)各70例的外周血样标本,提取DNA,采用等位基因聚合酶链反应(PCR-RFLP)的方法鉴定KCNE1(G38S)的基因型及等位基因分布。采用Logistic回归分析各种因素与房颤的相关性。结果 KCNE1基因G38S位点AA、AG、GG基因型频率在AF组分别为17.14%、27.14%、55.71%。在对照组分别为24.29%、50%、25.71%。2组基因型分布差异具有统计学意义(P<0.05),且AF组G等位基因频率明显高于对照组(P<0.05)。Logistic回归分析结果显示KCNE1(G38S)GG基因型与维吾尔族人群AF的发生相关(P<0.05)。结论新疆地区维吾尔族人群AF的发生与KCNE1(G38S)基因多态性相关。G38S位点多态性可能是维吾尔族AF患者的独立危险因素之一。
Objective To investigate the correlation between delayed rectifier potassium channel KCNE1( G38S)and atrial fibrillation( AF). Methods Seventy elderly Uygur patients with AF( AF group) and seventy elderly Uygur patients without AF( non-AF group) were enrolled in this study. Peripheral blood sample specimens were collected and DNA was extracted from all subjects. KCNE1( G38S) genotypes and allelic distribution were identificated with the polymerase chain reaction-extinction enzymes fragment length polymorphism( PCR-RFLP). The risk factors of AF were analyzed using Logistic regression analysis. Results The frequency of AA,AG and GG genotype was 17. 14%,27. 14%,55. 71% at KCNE1 gene( G38 S site) in AF group,compared with 24. 29%,50% and 25. 71% in the control group( P <0. 05),and G allele frequency was higher in AF group than that in the control group( P < 0. 05); Logistic regression analysis showed that KCNE1( G38S) GG genotype was associated with the occurrence of AF in Uygur elderly population( P <0. 05). Conclusions The results suggest that KCNE1( G38S) polymorphism may be one of the independent risk factors of AF in Uygur patients.
Objective To investigate the correlation between delayed rectifier potassium channel KCNE1( G38S)and atrial fibrillation( AF). Methods Seventy elderly Uygur patients with AF( AF group) and seventy elderly Uygur patients without AF( non-AF group) were enrolled in this study. Peripheral blood sample specimens were collected and DNA was extracted from all subjects. KCNE1( G38S) genotypes and allelic distribution were identificated with the polymerase chain reaction-extinction enzymes fragment length polymorphism( PCR-RFLP). The risk factors of AF were analyzed using Logistic regression analysis. Results The frequency of AA,AG and GG genotype was 17. 14%,27. 14%,55. 71% at KCNE1 gene( G38 S site) in AF group,compared with 24. 29%,50% and 25. 71% in the control group( P <0. 05),and G allele frequency was higher in AF group than that in the control group( P < 0. 05); Logistic regression analysis showed that KCNE1( G38S) GG genotype was associated with the occurrence of AF in Uygur elderly population( P <0. 05). Conclusions The results suggest that KCNE1( G38S) polymorphism may be one of the independent risk factors of AF in Uygur patients.
引文
[1]胡大一,周自强,陈捷,等.中国AF现状流行病学研究[J].中华内科杂志,2004,43(7):491-494.
[2]张丹凤,夏向南.老年人房颤临床分析[J].实用老年医学,2006,20(2):142-143.
[3]Brugada R,Tapscott T,Czernuszewicz GZ,et al.Identification of a genetic locus for familial atrial fibrillation[J].N Engl J Med,1997,336(13):905-911.
[4]戴芝银,刘金平,王玉,等.慢性房颤患者心房肌细胞KAch通道Kir3.4基因和蛋白表达变化及机制[J].山东医药,2010,50(5):1-3.
[5]Fatini C,Sticchi E,Genuardi M,et al.Analysis of mink and e NOS genes as candidate loci for predisposition to nonvalvular atrial fibrillation[J].Eur Heart J,2006,27(14):1712-1718.
[6]Schreieck J,Dostal S,Von Beckerathn,et al.C825T polymorphism of the G-protein beta3 subunit gene and atrial fibrillation:association of the TT genotype with a reduced risk for atrial fibrillation[J].AM Heart J,2004,148(3):545-550.
[7]Ackerman MJ,Tester DJ,Jones GS,et al.Ethnic differences in cardiac potassium channel variants:implications for genetic susceptibility to sudden cardiac death and genetic testing for congenital long QT syndrome[J].Mayo Clin Proc,2003,78(12):1479-1487.
[8]Lai LP,Su MJ,Yeh HM,et al.Association of the human Minx gene 38G allele with atrial fibrillation:evidence of possible genetic control on the pathogenesis of atrial fibrillation[J].Am Heart J,2002,144(3):485-490.
[9]Ehrlich JR,Zicha S,Coutu P,et al.Atrial fibrillation-associated Min K38G/S polymorphism modulates delayed rectifier current and membrane localization[J].Cardiovasc Res,2005,67(3):520-528.
[10]Prystupa A,Dzida G,Myslinski W,et al.Mink gene polymorphism in the pathogenesis of lone atrial fibrillation[J].Kardiol Pol,2006,64(11):1205-1211.
[11]Olson TM,Michels OV,Ballew JD,et al.Sodium channel mutations and susceptibility to heart failure and atrial fibrillation[J].JAMA,2005,293(4):447-454.
[12]Chen LY,Ballew JD,Herron KJ,et al.A common polymorphism in SCN5A is associated with lone atrial fibrillation[J].Clin Pharmacol Ther,2007,81(1):36.
[13]木胡牙提,卢武红,马依彤,等.汉族与维吾尔族AF患者临床特性的比较[J].中国心脏起搏与心电生理杂志,2008,22(3):216-218.
[14]马依彤,姚娟,谢翔,等.心肌钾离子通道β亚单位基因rs1805127多态性与新疆维吾尔族AF的相关性[J].中华医学遗传学杂志,2011,28(4):436-440.
[2]张丹凤,夏向南.老年人房颤临床分析[J].实用老年医学,2006,20(2):142-143.
[3]Brugada R,Tapscott T,Czernuszewicz GZ,et al.Identification of a genetic locus for familial atrial fibrillation[J].N Engl J Med,1997,336(13):905-911.
[4]戴芝银,刘金平,王玉,等.慢性房颤患者心房肌细胞KAch通道Kir3.4基因和蛋白表达变化及机制[J].山东医药,2010,50(5):1-3.
[5]Fatini C,Sticchi E,Genuardi M,et al.Analysis of mink and e NOS genes as candidate loci for predisposition to nonvalvular atrial fibrillation[J].Eur Heart J,2006,27(14):1712-1718.
[6]Schreieck J,Dostal S,Von Beckerathn,et al.C825T polymorphism of the G-protein beta3 subunit gene and atrial fibrillation:association of the TT genotype with a reduced risk for atrial fibrillation[J].AM Heart J,2004,148(3):545-550.
[7]Ackerman MJ,Tester DJ,Jones GS,et al.Ethnic differences in cardiac potassium channel variants:implications for genetic susceptibility to sudden cardiac death and genetic testing for congenital long QT syndrome[J].Mayo Clin Proc,2003,78(12):1479-1487.
[8]Lai LP,Su MJ,Yeh HM,et al.Association of the human Minx gene 38G allele with atrial fibrillation:evidence of possible genetic control on the pathogenesis of atrial fibrillation[J].Am Heart J,2002,144(3):485-490.
[9]Ehrlich JR,Zicha S,Coutu P,et al.Atrial fibrillation-associated Min K38G/S polymorphism modulates delayed rectifier current and membrane localization[J].Cardiovasc Res,2005,67(3):520-528.
[10]Prystupa A,Dzida G,Myslinski W,et al.Mink gene polymorphism in the pathogenesis of lone atrial fibrillation[J].Kardiol Pol,2006,64(11):1205-1211.
[11]Olson TM,Michels OV,Ballew JD,et al.Sodium channel mutations and susceptibility to heart failure and atrial fibrillation[J].JAMA,2005,293(4):447-454.
[12]Chen LY,Ballew JD,Herron KJ,et al.A common polymorphism in SCN5A is associated with lone atrial fibrillation[J].Clin Pharmacol Ther,2007,81(1):36.
[13]木胡牙提,卢武红,马依彤,等.汉族与维吾尔族AF患者临床特性的比较[J].中国心脏起搏与心电生理杂志,2008,22(3):216-218.
[14]马依彤,姚娟,谢翔,等.心肌钾离子通道β亚单位基因rs1805127多态性与新疆维吾尔族AF的相关性[J].中华医学遗传学杂志,2011,28(4):436-440.