不同蛋白源对瓦氏黄颡鱼幼鱼生长和蛋白质代谢相关基因表达的影响
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摘要
以初始体重为(2.90±0.01) g的瓦氏黄颡鱼(Pelteobagrus vachelli)为研究对象,探究不同蛋白源对瓦氏黄颡鱼生长、体组成、前肠PEPT1和肝脏TOR基因表达的影响。以鱼粉(FM)、大豆浓缩蛋白(SPC)、水解鱼蛋白(FH)和晶体氨基酸混合物(CAA)为主要蛋白源,配制4种等氮(粗蛋白含量为39.0%)等脂(粗脂肪含量为9.0%)的实验饲料,投喂实验鱼66d。结果显示,不同蛋白源虽然对瓦氏黄颡鱼存活率(SR)无显著影响(P>0.05),但显著影响了瓦氏黄颡鱼的特定生长率(SGR)。其中, SPC组瓦氏黄颡鱼SGR低于FM组,却显著高于FH组和CAA组(P<0.05)。虽然SPC组瓦氏黄颡鱼粗蛋白含量低于FM组,但其鱼体粗脂肪含量显著高于FM组(P<0.05)。不同蛋白源显著影响了瓦氏黄颡鱼PEPT1基因表达(P<0.05),在35d时, SPC组PEPT1表达量虽然低于FM组,但显著高于FH和CAA组(P<0.05);在66d时, SPC组瓦氏黄颡鱼前肠PEPT1表达量显著高于FM组(P<0.05)。不同蛋白源饲料对鱼体肝脏TOR基因表达无显著影响(P>0.05)。以上结果表明, SPC组瓦氏黄颡鱼生长性能虽然低于FM组,但显著高于FH组和CAA组,这可能是通过上调前肠PEPT1表达来实现的。
A 66-day feeding trial was conducted to investigate the effects of different protein sources on growth, body composition and PEPT1 gene expression in the foreintestine and TOR gene expression in the liver of juvenile darkbarbel catfish [initial weight(2.90 ± 0.01) g]. Four isoproteic(39.0% crude protein) and isolipidic(9.0% crude lipid) diets were formulated with fish meal(FM), soy protein concentrate(SPC), fish hydrolysate(FH) and crystal amino acids(CAA) as the main protein source. No significant difference was observed in the survival rate among dietary treatments(P>0.05). Dietary protein had a significant effect on SGR. SPC group had lower SGR than that of the FM groups, while SPC group had higher SGR than FH and CAA group(P<0.05). The crude protein of SPC group was only lower than the FM group, while the crude lipid of SPC group was higher than the FM group(P<0.05). Dietary protein had a significant effect on PEPT1 mRNA expression. The PEPT1 mRNA expression in SPC group was significantly lower than the FM group, but significantly higher than the FH and CAA group at day 35. However, the PEPT1 mRNA expression of SPC group was significantly higher than the FM group at day 66(P<0.05). Dietary protein source had no effect on TOR gene expression. These results suggested that juvenile darkbarbel catfish of SPC group had better growth performance than FH and CAA group, which might be attributed to the higher foregut PEPT1 mRNA expression.
A 66-day feeding trial was conducted to investigate the effects of different protein sources on growth, body composition and PEPT1 gene expression in the foreintestine and TOR gene expression in the liver of juvenile darkbarbel catfish [initial weight(2.90 ± 0.01) g]. Four isoproteic(39.0% crude protein) and isolipidic(9.0% crude lipid) diets were formulated with fish meal(FM), soy protein concentrate(SPC), fish hydrolysate(FH) and crystal amino acids(CAA) as the main protein source. No significant difference was observed in the survival rate among dietary treatments(P>0.05). Dietary protein had a significant effect on SGR. SPC group had lower SGR than that of the FM groups, while SPC group had higher SGR than FH and CAA group(P<0.05). The crude protein of SPC group was only lower than the FM group, while the crude lipid of SPC group was higher than the FM group(P<0.05). Dietary protein had a significant effect on PEPT1 mRNA expression. The PEPT1 mRNA expression in SPC group was significantly lower than the FM group, but significantly higher than the FH and CAA group at day 35. However, the PEPT1 mRNA expression of SPC group was significantly higher than the FM group at day 66(P<0.05). Dietary protein source had no effect on TOR gene expression. These results suggested that juvenile darkbarbel catfish of SPC group had better growth performance than FH and CAA group, which might be attributed to the higher foregut PEPT1 mRNA expression.
引文
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[13]Liu J W,Mai K S,Xu W,et al.Effects of dietary glutamine on activities of non-specific immune related enzymes and HIF-1a expression after hypoxia of halfsmooth tongue sole post larvae[J].Acta Hydrobiologica Sinica,2016,40(4):736-743[刘经纬,麦康森,徐玮,等.谷氨酰胺对半滑舌鳎稚鱼非特异性免疫相关酶活力和低氧应激后HIF-1a表达的影响.水生生物学报,2016,40(4):736-743]
[14]Wang Z,Xu W,Mai K S,et al.The effects of valine level on plasma biochemical indexes,lipid content and gene expression involved in lipid metabolism in cobia(Rachycentron canadum)[J].Acta Hydrobiologica Sinica,2016,40(4):744-751[王震,徐玮,麦康森,等.饲料缬氨酸水平对军曹鱼鱼体脂肪含量、血浆生化指标和肝脏脂肪代谢基因表达的影响.水生生物学报,2016,40(4):744-751]
[15]Zhang S,Ai Q H,Mai K S,et al.Effects of fish meal replacement with crystalline amino acid on digestive and metabolic enzymes of tongue sole(Cynoglossus semilaevis)larae[J].Acta Hydrobiologica Sinica,2014,38(5):801-808[张珊,艾庆辉,麦康森,等.晶体氨基酸替代鱼粉蛋白对半滑舌鳎稚鱼消化酶和代谢酶活力的影响.水生生物学报,2014,38(5):801-808]
[16]Terova G,Robaina L,Izquierdo M,et al.PepT1 mRNAexpression levels in sea bream(Sparus aurata)fed different plant protein sources[J].Springerplus,2013,2(1):1-14
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[21]Spanier B,Rohm F.Proton coupled oligopeptide transporter 1(PepT1)function,regulation,and influence on the intestinal homeostasis[J].Comprehensive Physiology,2018,8(2):843-869
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[25]Livak K J,Schmittgen T D.Analysis of relative gene expression data using real-time quantitative PCR and the2-ΔΔCt method[J].Methods,2001,25(4):402-408
[26]Aragao C,Lec C,Dias J,et al.Soy protein concentrate as a protein source for Senegalese sole(Solea senegalensis Kaup 1858)diets:effects on growth and amino acid metabolism of postlarvae[J].Aquaculture Research,2015,34(15):1443-1452
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