昆明小鼠主要脏器系数及生长指标的测定
|
摘要
目的通过测定KM小鼠体质量、主要脏器质量、体长、耳长、尾长、食管长,获得KM小鼠的部分生物学数据。方法随机选取1～8周龄的SPF(specific pathogen free)级KM小鼠,每周龄20只,雌雄各半,共160只,测定并计算小鼠脏器系数及相关生长指标。结果①影响KM小鼠主要脏器质量、脏器系数的因素是性别及周龄,成对脏器的左右无明显差异(P>0.05)。②雄性KM小鼠的主要脏器质量大于同龄的雌性KM小鼠,肝与肾的脏器系数有明显性别差异(P<0.05)。雌雄小鼠的体、尾、耳、食管长度及尾/体长比值、耳/体长比值、食管/体长比值比较差异均无统计学意义(P>0.05)。③各脏器质量随周龄增加均有所增长,脏器系数变化明显(P<0.05),体、尾、耳、食管长度随周龄增加均有一定时段的显著增长(P<0.05),尾/体长比值与食管/体长比值比较差异有统计学意义(P<0.05),耳/体长比值无明显改变(P>0.05)。结论本实验为SPF级KM小鼠生物学特性的标准化探索提供相关可靠数据。
Objective To obtain the part of biological data about KM mice by measuring the body mass,organ mass,body length,ear length,tail length and esophagus length of KM mice.Methods Tatal of 160 aged 1～8 weeks specific pathogen free(SPF)KM mice were randomly divided into eight groups according to the age of the week,with male and female being half,20 KM mice per week.Organ coefficients and related growth indices were measured and calculated.Results ①Gender and age were the main factors affecting main organ mass and organ coefficients in KM mice,and there was no significant difference between left and right sides of two pairs of organs(P>0.05);②The main organ mass of male KM mice was higher than that of female KM mice at the same age,and the organ coefficients showed obvious gender difference in liver and kidney(P<0.05).There was no significant difference in body mass,tail mass,ear mass,esophagus length,tail to body length ratio,ear to body length ratio,and esophagus to body length ratio between male mice and female mice(P>0.05);③The mass of each organ increased with the week age and showed significant difference in organ coefficients(P<0.05).Body length,tail length,ear length,esophageal length increased a certain period of growth with week age(P<0.05).Tail to body length ratio and esophagus to body length ratio were statistically different(P<0.05),and there was no obvious change in ear to body length ratio(P>0.05).Conclusion The experiment may provide relevant reliable data for the standardization exploration of biological characteristics of SPF KM mice.
Objective To obtain the part of biological data about KM mice by measuring the body mass,organ mass,body length,ear length,tail length and esophagus length of KM mice.Methods Tatal of 160 aged 1～8 weeks specific pathogen free(SPF)KM mice were randomly divided into eight groups according to the age of the week,with male and female being half,20 KM mice per week.Organ coefficients and related growth indices were measured and calculated.Results ①Gender and age were the main factors affecting main organ mass and organ coefficients in KM mice,and there was no significant difference between left and right sides of two pairs of organs(P>0.05);②The main organ mass of male KM mice was higher than that of female KM mice at the same age,and the organ coefficients showed obvious gender difference in liver and kidney(P<0.05).There was no significant difference in body mass,tail mass,ear mass,esophagus length,tail to body length ratio,ear to body length ratio,and esophagus to body length ratio between male mice and female mice(P>0.05);③The mass of each organ increased with the week age and showed significant difference in organ coefficients(P<0.05).Body length,tail length,ear length,esophageal length increased a certain period of growth with week age(P<0.05).Tail to body length ratio and esophagus to body length ratio were statistically different(P<0.05),and there was no obvious change in ear to body length ratio(P>0.05).Conclusion The experiment may provide relevant reliable data for the standardization exploration of biological characteristics of SPF KM mice.
引文
[1] 左谦益,宁磊.昆明小鼠生长发育指标及繁殖性能测定[J].中国实验动物学杂志,2001,11(4):199-202.
[2] 练有文,王晖,倪少凯,等.BALB/c系裸小鼠脏器重量、脏器系数的测定[J].中国比较医学杂志,2006,16(5):285-287.
[3] 田永路,于洪江,张希牧,等.5～7周龄SD和Wistar大鼠主要脏器系数及体尺的测定[J].实验动物科学,2009,26(6):21-25.
[4] 孙建新,安娟,连军.影响实验动物脏器重量及脏器系数因素分析[J].实验动物科学,2009,26(1):49-51.
[5] 项华,章晓玲,查捷,等.SD大鼠生长发育指标和主要脏器正常参考值的探讨[J].中国卫生检验杂志,2008,18(2):299-301.
[6] 徐龙建,唐贤丽.4～5周龄D2BF1小鼠的血液学参考值、主要脏器重量及脏器系数的测定[J].中国比较医学杂志,2007,17(3):151-153.
[7] 刘福剀,关雪,杨昕蕾,等.不同周龄BALB/c小鼠脏器质量及其变化趋势分析[J].现代生物医学进展,2014,14(3):421-424.
[8] 田小芸,恽时锋,胡玉红,等.基因工程ROSA小鼠主要脏器重量及脏器系数的测定[J].实验动物科学,2009,26(1):1-3.
[9] 黄小红,赵伟健,黄小琼,等.SPF级近交系BALB/c小鼠的体重、体长、尾长和脏器系数的测定[J].中国兽医杂志,2007,43(7):33-34.
[10] 郭建强,齐娜,苏忆兰,等.SPF级大鼠体重和主要脏器正常参考值的探讨[J].卫生毒理学杂志,2002,16(4):255-257.
[11] 卢胜明,胡建武,车路平,等.10种常用SPF级实验大、小鼠体重及主要脏器的测定[J].实验动物科学,2007,24(3):12-16.
[12] 张修彦,詹纯列,陈伟盛.SPF级KM、NIH、BALB/c小鼠生长指标的测定与比较[J].中国比较医学杂志,2013,23(3):45-48.
[13] 卢笑丛,彭定学,冯楚北.昆明种小鼠体长与尾长的测定[J].动物学杂志,1991,26(4):44-46.
[2] 练有文,王晖,倪少凯,等.BALB/c系裸小鼠脏器重量、脏器系数的测定[J].中国比较医学杂志,2006,16(5):285-287.
[3] 田永路,于洪江,张希牧,等.5～7周龄SD和Wistar大鼠主要脏器系数及体尺的测定[J].实验动物科学,2009,26(6):21-25.
[4] 孙建新,安娟,连军.影响实验动物脏器重量及脏器系数因素分析[J].实验动物科学,2009,26(1):49-51.
[5] 项华,章晓玲,查捷,等.SD大鼠生长发育指标和主要脏器正常参考值的探讨[J].中国卫生检验杂志,2008,18(2):299-301.
[6] 徐龙建,唐贤丽.4～5周龄D2BF1小鼠的血液学参考值、主要脏器重量及脏器系数的测定[J].中国比较医学杂志,2007,17(3):151-153.
[7] 刘福剀,关雪,杨昕蕾,等.不同周龄BALB/c小鼠脏器质量及其变化趋势分析[J].现代生物医学进展,2014,14(3):421-424.
[8] 田小芸,恽时锋,胡玉红,等.基因工程ROSA小鼠主要脏器重量及脏器系数的测定[J].实验动物科学,2009,26(1):1-3.
[9] 黄小红,赵伟健,黄小琼,等.SPF级近交系BALB/c小鼠的体重、体长、尾长和脏器系数的测定[J].中国兽医杂志,2007,43(7):33-34.
[10] 郭建强,齐娜,苏忆兰,等.SPF级大鼠体重和主要脏器正常参考值的探讨[J].卫生毒理学杂志,2002,16(4):255-257.
[11] 卢胜明,胡建武,车路平,等.10种常用SPF级实验大、小鼠体重及主要脏器的测定[J].实验动物科学,2007,24(3):12-16.
[12] 张修彦,詹纯列,陈伟盛.SPF级KM、NIH、BALB/c小鼠生长指标的测定与比较[J].中国比较医学杂志,2013,23(3):45-48.
[13] 卢笑丛,彭定学,冯楚北.昆明种小鼠体长与尾长的测定[J].动物学杂志,1991,26(4):44-46.