高效液相色谱法测定葡萄糖光催化氧化产物
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摘要
建立了一种定量检测葡萄糖及其氧化产物的高效液相色谱法。采用示差折光检测器(RID),Shodex SH1011色谱柱(8.0 mm×300 mm,6μm),流动相为0.24 mmol·L~(-1)硫酸,流速为0.6 mL·min~(-1),柱温为50℃,进样量为20μL。结果表明,葡萄糖及其氧化产物在线性范围内浓度与峰面积呈良好的线性关系,回收率为96.7%～103.3%,相对标准偏差为0.71%～2.73%。葡萄糖的氧化产物为葡萄糖二酸、葡萄糖酸、阿拉伯糖和甲酸。该方法能够对葡萄糖及其氧化产物进行准确的定量分析。
We established a method for the quantitative determination of glucose and its oxidative products by high performance liquid chromatography.In the process of determination,refractive index detector(RID) was adopted,Shodex SH1011 column(8.0 mm×300 mm,6 μm) was adopted,0.24 mmol·L~(-1) sulfuric acid was adopted as a mobile phase,and parameters,such as the flow rate of 0.6 mL·min~(-1),the column temperature of 50 ℃,and the injection volume of 20 μL,were adopted.The results indicate that the concentrations of glucose and its oxidation products show good linear relationship with the peak area in the linear range.The recovery is 96.7%-103.3%,and relative standard deviation is 0.71%-2.73%.Moreover,the oxidative products of glucose are glucaric acid,gluconic acid,arabinose,and formic acid.This method can be used for the exact quantitative analysis of glucose and its oxidation products.
We established a method for the quantitative determination of glucose and its oxidative products by high performance liquid chromatography.In the process of determination,refractive index detector(RID) was adopted,Shodex SH1011 column(8.0 mm×300 mm,6 μm) was adopted,0.24 mmol·L~(-1) sulfuric acid was adopted as a mobile phase,and parameters,such as the flow rate of 0.6 mL·min~(-1),the column temperature of 50 ℃,and the injection volume of 20 μL,were adopted.The results indicate that the concentrations of glucose and its oxidation products show good linear relationship with the peak area in the linear range.The recovery is 96.7%-103.3%,and relative standard deviation is 0.71%-2.73%.Moreover,the oxidative products of glucose are glucaric acid,gluconic acid,arabinose,and formic acid.This method can be used for the exact quantitative analysis of glucose and its oxidation products.
引文
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[16] da VIà L,RECCHI C,DAVIES T E,et al.Visible-light-controlled oxidation of glucose using titania-supported silver photocatalysts[J].ChemCatChem,2016,8(22):3475-3483.
[2] CHANG C C,GREEN S K,WILLIAMS C L,et al.Ultra-selective cycloaddition of dimethylfuran for renewable p-xylene with H-BEA [J].Green Chemistry,2014,16(2):585-588.
[3] BOZELL J J.Connecting biomass and petroleum processing with a chemical bridge[J].Science,2010,329(5991):522-523.
[4] BELLARDITA M,GARCíA-LóPEZ E I,MARCí G,et al.Photocatalytic formation of H2 and value-added chemicals in aqueous glucose(Pt)-TiO2 suspension[J].International Journal of Hydrogen Energy,2016,41(14):5934-5947.
[5] COLMENARES J C,MAGDZIARZ A,BIELEJEWSKA A.High-value chemicals obtained from selective photo-oxidation of glucose in the presence of nanostructured titanium photocatalysts[J].Bioresource Technology,2011,102(24):11254-11257.
[6] JIN X,ZHAO M,VORA M,et al.Synergistic effects of bimetallic PtPd/TiO2 nanocatalysts in oxidation of glucose to glucaric acid:structure dependent activity and selectivity[J].Industrial & Engineering Chemistry Research,2016,55:2932-2945.
[7] MEGíAS-SAYAGO C,IVANOVA S,LóPEZ-CARTES C,et al.Gold catalysts screening in base-free aerobic oxidation of glucose togluconic acid[J].Catalysis Today,2017,279:148-154.
[8] MOON T S,YOON S H,LANZA A M,et al.Production of glucaric acid from a synthetic pathway in recombinant Escherichia coli[J].Applied and Environmental Microbiology,2009,75(3):589-595.
[9] ISIKGOR F H,BECER C R.Lignocellulosic biomass:a sustainable platform for the production of bio-based chemicals and polymers[J].Polymer Chemistry,2015,6:4497-4559.
[10] WU Y X,ENOMOTO-ROGERS Y,MASAKI H,et al.Synthesis of crystalline and amphiphilic polymers from d-glucaric acid[J].ACS Sustainable Chemistry & Engineering,2016,4(7):3812-3819.
[11] 朱晓兰,高芸,苏庆德.毛细管气相色谱法测定番茄中的非挥发性有机酸、高级脂肪酸和低分子量糖类[J].食品科学,2004,25(9):152-157.ZHU X L,GAO Y,SU Q D.Determination of nonvolatile organic acid,fatty acid and low molecular sugars in tomato by capillary gas chromatography[J].Food Science,2004,25(9):152-157.
[12] 符梦凡,赵一帆,阎卫东.柱前衍生化HPLC法分析枸杞多糖中单糖组成 [J].食品科学,2018,39(18):186-191.FU M F,ZHAO Y F,YAN W D.Analysis of monosaccharide composition of polysaccharides from Lycium barbarum L.by HPLC with precolumn derivatization[J].Food Science,2018,39(18):186-191.
[13] 朱璇,余作龙,周佳栋,等.反相离子对液相色谱测定葡萄糖化学氧化过程中的6种有机酸[J].分析试验室,2013,32(5):20-24.ZHU X,YU Z L,ZHOU J D,et al.Determination of six organic acids in chemical oxidation process of glucose by ion-pair reversed phase HPLC[J].Chinese Journal of Analysis Laboratory,2013,32(5):20-24.
[14] SOLMI S,MORREALE C,OSPITALI F,et al.Oxidation of D-glucose to glucaric acid using Au/C catalysts[J].ChemCatChem,2017,9(14):2797-2806.
[15] CHONG R F,LI J,MA Y,et al.Selective conversion of aqueous glucose to value-added sugar aldose on TiO2-based photocatalysts[J].Journal of Catalysis,2014,314:101-108.
[16] da VIà L,RECCHI C,DAVIES T E,et al.Visible-light-controlled oxidation of glucose using titania-supported silver photocatalysts[J].ChemCatChem,2016,8(22):3475-3483.