p(AA-co-CTS)两性型规整吸附介质的制备及动态吸附性能
|
摘要
以丙烯酸(AA)、壳聚糖(CTS)为单体,选用抗坏血酸(VC)为还原剂取代传统胺类还原剂,通过低温冷冻聚合法一步制备了富含—COOH基团和—NH2基团的两性型规整吸附介质p(AA-co-CTS),聚合时间从文献报道的18h缩短至5h。通过SEM对p(AA-co-CTS)规整吸附介质进行表征,其内部孔隙连续,孔径范围为100～500μm。聚合最优配方为:引发剂过硫酸铵(APS)与VC总质量分数为0.75%(以聚合体系总质量计,下同),VC与APS的质量比为0.5∶1.0,单体总质量分数为7%(以聚合体系总质量计,下同),m(CTS)∶m(AA)=0.2∶1.0,交联剂N,N?-亚甲基双丙烯酰胺(MBA)质量分数为1.05%(以聚合体系总质量计,下同)。借助吸附传质单元数(NTU)法探究了传质参数与多肽吸附操作条件的关系,并确定最佳吸附条件为:吸附液流速3～4 mL/min,吸附温度293.15 K,进料液质量浓度400～500 mg/L。所制备的两性型分离介质属于超大孔隙规整介质,最大动态吸附容量为74.5～79.4 mg/g,应用上可取代传统吸附工艺中需过滤、离心及层析等单元操作的散装吸附树脂介质。
A continuous amphoteric adsorption medium p(AA-co-CTS) rich in carboxyl and amino groups was prepared by one step copolymerization using acrylic acid(AA) and chitosan(CTS) as raw materials,and ascorbic acid(VC) as reducing agent instead of traditional amine reductant. The polymerization time was shortened from 18 hours reported in literature to 5 hours. SEM showed that the pore structure of p(AA-co-CTS) adsorption medium prepared was continuous and the pore size scale was 100～500 μm. The optimum polymerization parameters were as follows: ammonium persulfate(ASP) and VC as initiator had0.75% total mass fraction, m(VC)∶m(APS)=0.5∶1.0. Monomers had 7% mass fraction, m(CTS)∶m(AA)=0.2∶1.0. Cross-linking agent N, N'-methylene bisacrylamide(MBA) had 1.05% mass fraction.The relationship between mass transfer parameters and the operation conditions of polypeptide adsorption was studied by number of mass transfer units(NTU) method. The optimum adsorption operation conditions were determined as follows: the flow rate of adsorption liquid was 3～4 mL/min, the adsorption temperature was 293.15 K, and the feed concentration was 400～500 mg/L. The prepared amphoteric separation medium was a super porous continuous medium with a maximum dynamic adsorption capacity of 74.5～79.4 mg/g, which could replace bulk adsorption resin medium in the traditional adsorption process of filtering, centrifugation and chromatography and other units operations.
A continuous amphoteric adsorption medium p(AA-co-CTS) rich in carboxyl and amino groups was prepared by one step copolymerization using acrylic acid(AA) and chitosan(CTS) as raw materials,and ascorbic acid(VC) as reducing agent instead of traditional amine reductant. The polymerization time was shortened from 18 hours reported in literature to 5 hours. SEM showed that the pore structure of p(AA-co-CTS) adsorption medium prepared was continuous and the pore size scale was 100～500 μm. The optimum polymerization parameters were as follows: ammonium persulfate(ASP) and VC as initiator had0.75% total mass fraction, m(VC)∶m(APS)=0.5∶1.0. Monomers had 7% mass fraction, m(CTS)∶m(AA)=0.2∶1.0. Cross-linking agent N, N'-methylene bisacrylamide(MBA) had 1.05% mass fraction.The relationship between mass transfer parameters and the operation conditions of polypeptide adsorption was studied by number of mass transfer units(NTU) method. The optimum adsorption operation conditions were determined as follows: the flow rate of adsorption liquid was 3～4 mL/min, the adsorption temperature was 293.15 K, and the feed concentration was 400～500 mg/L. The prepared amphoteric separation medium was a super porous continuous medium with a maximum dynamic adsorption capacity of 74.5～79.4 mg/g, which could replace bulk adsorption resin medium in the traditional adsorption process of filtering, centrifugation and chromatography and other units operations.
引文
[1]Zhuang Yongliang(庄永亮).Study on the biochemical characterization of collagen and skin care activity of collagen peptides extracted from jellyfish rhopilema esculentum[D].Qingdao:Ocean University of China(中国海洋大学),2009.
[2]Rushikesh S,Pravin P,Seetharama J.Peptides,peptidomimetics,and polypeptides from marine sources:A wealth of natural sources for pharmaceutical applications[J].Marine Drugs,2017,15(124):1-37.
[3]Velusamy A,Manigandan V,Saravanan R.et al.Bioactive peptides from marine ascidians and future drug development-A review[J].International Journal of Peptide Research and Therapeutics,2018,24,(1):13-18.
[4]Yang H,Wang Y,Zhou P,et al.Effects of alkaline and acid peteatment on the physical properties and nanostructures of the gelatin from channel catfish skins[J].Food Hydrocolloide,2008,22(8):1541-1550.
[5]Fang W Y,Dahiya R,Qin H L,et al.Natural proline-rich cyclopolypeptides from marine organisms:Chemistry,synthetic methodologies and biological status[J].Marine Drugs,2016,14(194):1-22.
[6]Daniela G,Maria C,Daniela C,et al.Biotechnological applications of bioactive peptides from marine sources[J].Advances in Microbial Physiology,2018,73:171-220.
[7]Assaad S,Ali B.Antioxidant peptides from marine by-products:isolation,identification and application in food systems.A review[J].Foods,2016,21(3):10-26.
[8]Dan N,Ganesan R,Flood K G,et al.Determination of enantiomers in a synthetic argininal peptide using capillary zone electrophoresis and high-performance liquid chromatography[J].Journal of Chromatography A,2000,891(1):115-127.
[9]Sanz N V,Benavente F,Toro I,et al.Optimization of HPLCconditions for the separation of complex crude mixtures produced in the synthesis of the therapeutic peptide homones[J].Chromatographia,2001,53(1):S167-S173.
[10]Lozinsky V I.Polymeric cryogels as a new family of macroporous and supermacroporous materials for biotechnological purposes[J].Russian Chemical Bulletin,2008,57(5):1015-1032.
[11]Zhan X Y,Lu D P,Lin D Q,et al.Preparation and characterization of supermacroporous polyacrylamide cryogel beads for biotechnological application[J].Journal of Applied Polymer Science,2013,130(5):3082-3089.
[12]Mendis E,Rajapakse N,Kim S K.Antioxidant properties of a radical-scavenging peptide purified from enzymatically prepared fish skin gelatin hydrolysate[J].Journal of Agricultural and Food Chemistry,2005,53(3):581-587.
[13]Mendis E,Rajapakse N,Byun H G,et al.Investigation of jumbo squid(Dosidicus gigas)skin gelatin peptides for their in vitro antioxidant effects[J].Life Sciences,2005,77(17):2166-2178.
[14]Aevidsson P,Plieva F M,Savina I N,et al.Chromatography of microbial cells using continuous supermacroporous affinity and ion-exchange columns[J].Journal of Chromatography A,2002,977(1):27-38.
[15]Aevidsson P,Plieva F M,Lozinsky V I,et al.Direct chromatographic capture of enzyme from crude homogenate using immobilized metal affinity chromatography on a continuous supermacroporous adsorbent[J].Journal of Chromatography A,2003,986(2):275-290.
[16]Persson P,Baybak O,Plieva F,et al.Characterization of a continuous supermacroporous monolithic matrix for chromatographic separation of large bioparticles[J].Biotechnology and Bioengineering,2010,88(2):224-236.
[17]Plieva F M,Andersson J,Galaev I Y,et al.Characterization of polyacrylamide based monolithic columns[J].Journal of Separation Science,2004,27(10/11):828-836.
[18]Aevidsson P,Plieva F M,Lozinsky V I,et al.Direct chromatography capture of enzyme from crude homogenate using immobilized metal affinity chromatography on a continuous supermacroporous adsorbent[J].Journal of Chromatography A,2003,986(2):275-290.
[19]Yao K J,Shen S C,Yun J X,et al.Preparation of polyacrylamidebased supermacroporous monolithic cryogel beds under freezingtemperature variation conditions[J].Chemical Engineering Science,2006,61(20):6701-6708.
[20]Yao K J,Yun J X,Shen S C,et al.Protein adsorption in supermacroporous cryogels with embedded nanoparticles[J].Biochemical Engineering Journal,2007,36(2):139-146.
[21]Liu Jie(刘杰),Shen Shaochuan(沈绍传),Chen Ping(陈平),et al.Separation of cytidine triphosphate from Saccharomyces cerevisiae broth by anion exchange poly(2-hydroxyethyl methacrylate)composite cryogel embedded with SiO2 nanoparticles[J].CIESCJournal(化工学报),2014,65(10):3938-3944.
[22]Guo Yantao(郭延涛),Shen Shaochuan(沈绍传),Yun Junxian(贠军贤),et al.Chromatographic separation of plasmid DNA by anion-exchange cryogel[J].Chinese Journal of Biotechnology(生物工程学报),2012,28(8):995-1001.
[23]Chen F,Yao K J,Yun J X,et al.Influence of grafting conditions on the properties of polyacrylamide-based cation-exchange cryogels grafted with 2-acrylamido-2-methyl-1-propanesulfonic acid[J].Chemical Engineering Science,2008,(63):71-77.
[24]Yun J X,Jepspersen G R,Kirsebom H,et al.An improved capillary model for describing the microstructure characteristics,fluid hydrodynamics and breakthrough performance of proteins in cryogel beds[J].Journal of Chromatography A,2011,1218(32):5487-5497.
[25]Ye Zhenhua(叶振华).Chemical adsorption separation process(化工吸附分离过程)[M].Beijing:China Petrochemical Press(中国石化出版社),1992:131-134.
[26]Kitagawa Hiroshi[(日)北川浩],Akihiro Suzuki[(日)铃木谦一郎].Fundamentals and design of adsorption(吸附的基础与设计),Lu Zhengli[鹿政理(译)][M].Beijing:Chemical Industry Press(化学工业出版社),1983:79-110.
[27]He Zhao(何钊),Ren Qilong(任其龙).Separation of proanthocyanidin from extracts of grape seeds by chromatographe[J].Food Science(食品科学),2004,25(8):70-72.
[28]Zhao Ping(赵平),Zhang Yueping(张月萍),Ren Peng(任鹏).Adsorption process of proanthocyanidins in AB-8 macroporous resin[J].CIESC Jorunal(化工学报),2013,64(3):980-985.
[29]National Technical Committee for Standardization of Special Diet(全国特殊膳食标准化技术委员会).GB/T22729-2008:Oligopeptides powder of marine fish(海洋鱼低聚肽粉)[S].Standards Press of China(中国标准出版社),2008:1-10.
[2]Rushikesh S,Pravin P,Seetharama J.Peptides,peptidomimetics,and polypeptides from marine sources:A wealth of natural sources for pharmaceutical applications[J].Marine Drugs,2017,15(124):1-37.
[3]Velusamy A,Manigandan V,Saravanan R.et al.Bioactive peptides from marine ascidians and future drug development-A review[J].International Journal of Peptide Research and Therapeutics,2018,24,(1):13-18.
[4]Yang H,Wang Y,Zhou P,et al.Effects of alkaline and acid peteatment on the physical properties and nanostructures of the gelatin from channel catfish skins[J].Food Hydrocolloide,2008,22(8):1541-1550.
[5]Fang W Y,Dahiya R,Qin H L,et al.Natural proline-rich cyclopolypeptides from marine organisms:Chemistry,synthetic methodologies and biological status[J].Marine Drugs,2016,14(194):1-22.
[6]Daniela G,Maria C,Daniela C,et al.Biotechnological applications of bioactive peptides from marine sources[J].Advances in Microbial Physiology,2018,73:171-220.
[7]Assaad S,Ali B.Antioxidant peptides from marine by-products:isolation,identification and application in food systems.A review[J].Foods,2016,21(3):10-26.
[8]Dan N,Ganesan R,Flood K G,et al.Determination of enantiomers in a synthetic argininal peptide using capillary zone electrophoresis and high-performance liquid chromatography[J].Journal of Chromatography A,2000,891(1):115-127.
[9]Sanz N V,Benavente F,Toro I,et al.Optimization of HPLCconditions for the separation of complex crude mixtures produced in the synthesis of the therapeutic peptide homones[J].Chromatographia,2001,53(1):S167-S173.
[10]Lozinsky V I.Polymeric cryogels as a new family of macroporous and supermacroporous materials for biotechnological purposes[J].Russian Chemical Bulletin,2008,57(5):1015-1032.
[11]Zhan X Y,Lu D P,Lin D Q,et al.Preparation and characterization of supermacroporous polyacrylamide cryogel beads for biotechnological application[J].Journal of Applied Polymer Science,2013,130(5):3082-3089.
[12]Mendis E,Rajapakse N,Kim S K.Antioxidant properties of a radical-scavenging peptide purified from enzymatically prepared fish skin gelatin hydrolysate[J].Journal of Agricultural and Food Chemistry,2005,53(3):581-587.
[13]Mendis E,Rajapakse N,Byun H G,et al.Investigation of jumbo squid(Dosidicus gigas)skin gelatin peptides for their in vitro antioxidant effects[J].Life Sciences,2005,77(17):2166-2178.
[14]Aevidsson P,Plieva F M,Savina I N,et al.Chromatography of microbial cells using continuous supermacroporous affinity and ion-exchange columns[J].Journal of Chromatography A,2002,977(1):27-38.
[15]Aevidsson P,Plieva F M,Lozinsky V I,et al.Direct chromatographic capture of enzyme from crude homogenate using immobilized metal affinity chromatography on a continuous supermacroporous adsorbent[J].Journal of Chromatography A,2003,986(2):275-290.
[16]Persson P,Baybak O,Plieva F,et al.Characterization of a continuous supermacroporous monolithic matrix for chromatographic separation of large bioparticles[J].Biotechnology and Bioengineering,2010,88(2):224-236.
[17]Plieva F M,Andersson J,Galaev I Y,et al.Characterization of polyacrylamide based monolithic columns[J].Journal of Separation Science,2004,27(10/11):828-836.
[18]Aevidsson P,Plieva F M,Lozinsky V I,et al.Direct chromatography capture of enzyme from crude homogenate using immobilized metal affinity chromatography on a continuous supermacroporous adsorbent[J].Journal of Chromatography A,2003,986(2):275-290.
[19]Yao K J,Shen S C,Yun J X,et al.Preparation of polyacrylamidebased supermacroporous monolithic cryogel beds under freezingtemperature variation conditions[J].Chemical Engineering Science,2006,61(20):6701-6708.
[20]Yao K J,Yun J X,Shen S C,et al.Protein adsorption in supermacroporous cryogels with embedded nanoparticles[J].Biochemical Engineering Journal,2007,36(2):139-146.
[21]Liu Jie(刘杰),Shen Shaochuan(沈绍传),Chen Ping(陈平),et al.Separation of cytidine triphosphate from Saccharomyces cerevisiae broth by anion exchange poly(2-hydroxyethyl methacrylate)composite cryogel embedded with SiO2 nanoparticles[J].CIESCJournal(化工学报),2014,65(10):3938-3944.
[22]Guo Yantao(郭延涛),Shen Shaochuan(沈绍传),Yun Junxian(贠军贤),et al.Chromatographic separation of plasmid DNA by anion-exchange cryogel[J].Chinese Journal of Biotechnology(生物工程学报),2012,28(8):995-1001.
[23]Chen F,Yao K J,Yun J X,et al.Influence of grafting conditions on the properties of polyacrylamide-based cation-exchange cryogels grafted with 2-acrylamido-2-methyl-1-propanesulfonic acid[J].Chemical Engineering Science,2008,(63):71-77.
[24]Yun J X,Jepspersen G R,Kirsebom H,et al.An improved capillary model for describing the microstructure characteristics,fluid hydrodynamics and breakthrough performance of proteins in cryogel beds[J].Journal of Chromatography A,2011,1218(32):5487-5497.
[25]Ye Zhenhua(叶振华).Chemical adsorption separation process(化工吸附分离过程)[M].Beijing:China Petrochemical Press(中国石化出版社),1992:131-134.
[26]Kitagawa Hiroshi[(日)北川浩],Akihiro Suzuki[(日)铃木谦一郎].Fundamentals and design of adsorption(吸附的基础与设计),Lu Zhengli[鹿政理(译)][M].Beijing:Chemical Industry Press(化学工业出版社),1983:79-110.
[27]He Zhao(何钊),Ren Qilong(任其龙).Separation of proanthocyanidin from extracts of grape seeds by chromatographe[J].Food Science(食品科学),2004,25(8):70-72.
[28]Zhao Ping(赵平),Zhang Yueping(张月萍),Ren Peng(任鹏).Adsorption process of proanthocyanidins in AB-8 macroporous resin[J].CIESC Jorunal(化工学报),2013,64(3):980-985.
[29]National Technical Committee for Standardization of Special Diet(全国特殊膳食标准化技术委员会).GB/T22729-2008:Oligopeptides powder of marine fish(海洋鱼低聚肽粉)[S].Standards Press of China(中国标准出版社),2008:1-10.