Kruppel样因子15对脂肪沉积和肌纤维类型的影响及其可能机制
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摘要
Kruppel样因子15(KLF15)是一种新发现的Kruppel样因子家族(KLFs)成员,为一种真核锌指蛋白转录因子。KLF15在动物体内呈现多组织表达特性,该基因过表达能促进动物脂肪沉积,且能影响肉质性状候选基因和Ⅰ型肌纤维基因的表达。因此,深入研究KLF15对脂肪沉积和肌纤维类型的影响及其可能机制,将为改善动物肉品质提供新的思路。本文综述了KLF15的发现、结构特征、表达规律及其与脂肪沉积和Ⅰ型肌纤维之间的关系。
Kruppel-like factor 15( KLF15) is a recently discovered member of Kruppel-like factors( KLFs)family,which serves as an important transcription factor,and is expressed in a variety of tissues. KLF15 was reported to promote fat deposition and type Ⅰ muscle fiber gene expression,and some other meat quality associated genes were also found to be regulated by KLF15. Study on the effects of KLF15 gene on fat deposition and muscle fiber types and its possible molecular mechanisms can provide a new thread of thought for improving the meat quality. This review summarized the discovery,structures and distribution of KLF15 and its roles in lipid deposition and type Ⅰ muscle fiber.
Kruppel-like factor 15( KLF15) is a recently discovered member of Kruppel-like factors( KLFs)family,which serves as an important transcription factor,and is expressed in a variety of tissues. KLF15 was reported to promote fat deposition and type Ⅰ muscle fiber gene expression,and some other meat quality associated genes were also found to be regulated by KLF15. Study on the effects of KLF15 gene on fat deposition and muscle fiber types and its possible molecular mechanisms can provide a new thread of thought for improving the meat quality. This review summarized the discovery,structures and distribution of KLF15 and its roles in lipid deposition and type Ⅰ muscle fiber.
引文
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[17]吕世杰.鸡KLF15基因的组织表达特性及多态性分析研究[D].硕士学位论文.郑州:河南农业大学,2014:13-19.
[18]王杰,陈婷,沈清武.KLF15基因表达特征及其腺病毒超表达载体的构建[J].西北农林科技大学学报:自然科学版,2015,43(7):35-40.
[19]FEI Z L,BERA T K,LIU X F,et al.Ankrd26 gene disruption enhances adipogenesis of mouse embryonic fibroblasts[J].The Journal of Biological Chemistry,2011,286(31):27761-27768.
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[21]BOONE C,MOUROT J,GRGOIRE F,et al.The adipose conversion process:regulation by extracellular and intracellular factors[J].Reproduction Nutrition Development,2000,40(4):325-358.
[22]WANG N D,FINEGOLD M J,BRADLEY A,et al.Impaired energy homeostasis in C/EBP alpha knockout mice[J].Science,1995,269(5227):1108-1112.
[23]SCHUPP M,LAZAR M A.Fingered for a fat fate[J].Cell M etabolism,2010,11(4):244-245.
[24]ANTONIADE C,ANTONOPOULO A S,TOUSOULI D,et al.Adiponectin:from obesity to cardiovascular disease[J].Obesity Review s,2009,10(3):269-279.
[25]KERSHAW E E,HAMM J K,VERHAGEN L A W,et al.Adipose triglyceride lipase:function,regulation by insulin,and comparison w ith adiponutrin[J].Diabetes,2006,55(1):148-157.
[26]SHAO D L,LAZAR M A.Peroxisome proliferator activated receptorγ,CCAAT/enhancer-binding Proteinα,and cell cycle status regulate the commitment to adipocyte differentiation[J].The Journal of Biological Chemistry,1997,272(34):21473-21478.
[27]EMNETT R S,GRINDFLEK E,ROTHSCHILD M F,et a1.Peroxisome proliferator-activated receptor gamma(PPARγ):a candidate gene for carcass performance and muscle eating quality in sw ine[J].Journal of Animal Science,2000,78:29-35.
[28]郝称莉,李齐发,乔永,等.湖羊肌肉组织H-FABP和PPARγ基因表达水平与肌内脂肪含量的相关研究[J].中国农业科学,2008,41(11):3776-3783.
[29]陈哲,王公金,于建宁,等.PPARγ2基因外显子1多态性对猪胴体性状的影响[J].江苏农业学报,2012,28(2):332-336.
[30]江书忠.猪脂肪沉积关键基因的筛选及锌指蛋白KLF13的功能研究[D].博士学位论文.武汉:华中农业大学,2014:17.
[31]张爱朋,王守志,王启贵,等.鸡C/EBPα基因的多态性与生长和体组成性状的相关研究[J].农业生物技术学报,2010,18(4):746-752.
[32]田磊磊.脂联素在皖南花猪脂肪中的表达及对肌内脂肪的影响[D].硕士学位论文.合肥:安徽农业大学,2013:17-19.
[33]张辉,李鹏,闫峰,等.不同品种肉牛脂联素表达与肌内脂肪沉积的关系[J].畜牧科学,2011(1):36-39.
[34]朱小玥.KLF15对AdipoRl表达的转录调控研究[D].硕士学位论文.武汉:华中农业大学,2012:32-45.
[35]刘培峰,冯佳炜,段晓雪,等.肌纤维类型及其转化对畜禽肉品质的影响[J].中国料,2015(16):16-19.
[36]李欣蔚,张勇.影响肉品嫩度的因素及其候选基因的研究进展[J].猪业科学,2011(7):106-108.
[37]IM S H,RAO A.Activation and deactivation of gene expression by Ca2+/calcineurin-NFAT-mediated signaling[J].M olecules and Cell,2004,18(1):1-9.
[2]郭佳.金华猪和长白猪肌纤维组成差异及ERK基因对肌纤维转化的影响研究[D].博士学位论文.杭州:浙江大学,2011:59-63.
[3]REALINI C E,VNIEN A,GOU P,et al.Characterization of Longissimus thoracis,Semitendinosus and Masseter muscles and relationships w ith technological quality in pigs.1.M icroscopic analysis of muscles[J].M eat Science,2013,94(3):408-416.
[4]UCHIDA S,SASAKI S,MARUMO F.Isolation of a novel zinc finger repressor that regulates the kidneyspecific CLC-K1 promoter[J].Kidney International,2001,60(2):416-421.
[5]MORI T,SAKAUE H,IGUCHI H,et al.Role of krüppel-like factor 15(KLF15)in transcriptional regulation of adipogenesis[J].The Journal of Biological Chemistry,2005,280(13):12867-12875.
[6]龙勤强,朱小玥,雷霆,等.猪MSCs细胞的成脂分化及KLF15对脂联素表达调控[C]//第十六次全国动物遗传育种学术讨论会论文集.扬州:中国畜牧兽医学会,2011:199.
[7]高欣.猪PNPLA3、4基因的克隆及功能研究[D].硕士学位论文.武汉:华中农业大学,2009:40-42.
[8]OISHI Y,MANABE I,TOBE K,et al.Krüppel-like transcription factor KLF5 is a key regulator of adipocyte differentiation[J].Cell M etabolism,2005,1(1):27-39.
[9]李青莹.KLF15对猪前体脂肪细胞分化及通过其含量预测脂肪沉积能力的研究[D].硕士学位论文.长春:吉林大学,2015:17-34.
[10]WANG J,CHEN T,FENG F,et al.KLF15 regulates slow myosin heavy chain expression through NFATc1in C2C12 myotubes[J].Biochemical and Biophysical Research Communications 2014,446(4):1231-1236.
[11]SUZUKI T,AIZAWA L,MATSUMURA T,et a1.Vascular implications of the Krüppel-like family of transcription factors[J].Arteriosclerosis,Thrombosis,and Vascular Biology,2005,25(6):1135-1141.
[12]杨红文,陈知龙.分歧多于保守:人、小鼠、大鼠和猪中klf家族进化分析[J].云南农业大学学报,2012,27(4):508-514.
[13]陈知龙.猪KLF家族基因克隆及分子特性研究[D].博士学位论文.武汉:华中农业大学,2010:73-81.
[14]KNOEDLER J R,DENVER R J.Krüppel-like factors are effectors of nuclear receptor signaling[J].General and Comparative Endocrinology,2014,203:49-59.
[15]王杰.KLF15通过NFATc1调节C2C12细胞慢肌球蛋白表达的研究[D].硕士学位论文.杨凌:西北农林科技大学,2014:17-26.
[16]李丽,朱伟,魏盟.Kruppel样因子15-多功能转录因子的功能研究[J].医学综述,2014,20(16):2916-2918.
[17]吕世杰.鸡KLF15基因的组织表达特性及多态性分析研究[D].硕士学位论文.郑州:河南农业大学,2014:13-19.
[18]王杰,陈婷,沈清武.KLF15基因表达特征及其腺病毒超表达载体的构建[J].西北农林科技大学学报:自然科学版,2015,43(7):35-40.
[19]FEI Z L,BERA T K,LIU X F,et al.Ankrd26 gene disruption enhances adipogenesis of mouse embryonic fibroblasts[J].The Journal of Biological Chemistry,2011,286(31):27761-27768.
[20]HALDAR S M,JEYARAJ D,ANAND P,et al.Kruppel-like factor 15 regulates skeletal muscle lipid flux and exercise adaptation[J].Proceedings of the National Academy of Sciences of the United States of America,2012,109(17):6739-6744.
[21]BOONE C,MOUROT J,GRGOIRE F,et al.The adipose conversion process:regulation by extracellular and intracellular factors[J].Reproduction Nutrition Development,2000,40(4):325-358.
[22]WANG N D,FINEGOLD M J,BRADLEY A,et al.Impaired energy homeostasis in C/EBP alpha knockout mice[J].Science,1995,269(5227):1108-1112.
[23]SCHUPP M,LAZAR M A.Fingered for a fat fate[J].Cell M etabolism,2010,11(4):244-245.
[24]ANTONIADE C,ANTONOPOULO A S,TOUSOULI D,et al.Adiponectin:from obesity to cardiovascular disease[J].Obesity Review s,2009,10(3):269-279.
[25]KERSHAW E E,HAMM J K,VERHAGEN L A W,et al.Adipose triglyceride lipase:function,regulation by insulin,and comparison w ith adiponutrin[J].Diabetes,2006,55(1):148-157.
[26]SHAO D L,LAZAR M A.Peroxisome proliferator activated receptorγ,CCAAT/enhancer-binding Proteinα,and cell cycle status regulate the commitment to adipocyte differentiation[J].The Journal of Biological Chemistry,1997,272(34):21473-21478.
[27]EMNETT R S,GRINDFLEK E,ROTHSCHILD M F,et a1.Peroxisome proliferator-activated receptor gamma(PPARγ):a candidate gene for carcass performance and muscle eating quality in sw ine[J].Journal of Animal Science,2000,78:29-35.
[28]郝称莉,李齐发,乔永,等.湖羊肌肉组织H-FABP和PPARγ基因表达水平与肌内脂肪含量的相关研究[J].中国农业科学,2008,41(11):3776-3783.
[29]陈哲,王公金,于建宁,等.PPARγ2基因外显子1多态性对猪胴体性状的影响[J].江苏农业学报,2012,28(2):332-336.
[30]江书忠.猪脂肪沉积关键基因的筛选及锌指蛋白KLF13的功能研究[D].博士学位论文.武汉:华中农业大学,2014:17.
[31]张爱朋,王守志,王启贵,等.鸡C/EBPα基因的多态性与生长和体组成性状的相关研究[J].农业生物技术学报,2010,18(4):746-752.
[32]田磊磊.脂联素在皖南花猪脂肪中的表达及对肌内脂肪的影响[D].硕士学位论文.合肥:安徽农业大学,2013:17-19.
[33]张辉,李鹏,闫峰,等.不同品种肉牛脂联素表达与肌内脂肪沉积的关系[J].畜牧科学,2011(1):36-39.
[34]朱小玥.KLF15对AdipoRl表达的转录调控研究[D].硕士学位论文.武汉:华中农业大学,2012:32-45.
[35]刘培峰,冯佳炜,段晓雪,等.肌纤维类型及其转化对畜禽肉品质的影响[J].中国料,2015(16):16-19.
[36]李欣蔚,张勇.影响肉品嫩度的因素及其候选基因的研究进展[J].猪业科学,2011(7):106-108.
[37]IM S H,RAO A.Activation and deactivation of gene expression by Ca2+/calcineurin-NFAT-mediated signaling[J].M olecules and Cell,2004,18(1):1-9.