KLF8及Snail1在结肠癌中的表达及意义
|
摘要
目的:探讨结肠癌组织中Krüppel样转录因子8(KLF8)、锌指蛋白转录因子1(Snail1)的表达及临床意义。方法:收集2015年11月—2017年11月70例结肠癌组织与30例癌旁正常组织,采用免疫组织化学法检测上述组织中KLF8、Snail1的表达情况,分析两者的相关性及临床病理特征的关系。结果:KLF8在结肠癌组织中的阳性表达率为71. 4%,高于癌旁正常组织20%,差异有统计学意义(P <0. 05); Snail1在结肠癌组织中的阳性表达率为54. 2%,高于癌旁正常组织的30%,差异有统计学意义(P <0. 05)。KLF8和Snail1在结肠癌组织中的表达与TNM分期、浸润深度及淋巴结转移有关(P <0. 05),与年龄、性别、肿瘤大小、分化程度无关(P> 0. 05)。在结肠癌组织中两者表达不存在相关性。结论:KLF8和Snail1在结肠癌组织中高表达,且两者之间不存在相关性,提示KLF8和Snail1两者通过不同的途径参与结肠癌的发生、发展过程。
Objective: To investigate the expression and clinical significance of Krüppel like factor 8( KLF8) and Zinc finger protein transcription factor 1( Snail1) in colon cancer tissues. Methods: 70 cases of colon cancer and 30 adjacent normal tissues were collected. Immunohistochemical method was used to determine the expression of KLF8 and Snail1 in those tissues.The correlation of the two factors in colon cancer tissues,and the relationship of the two factors with pathological features were analyzed. Results: The expression of KLF8( 71. 4%) in colon cancer tissues was significantly higher than that in adjacent normal tissues( 20%) with statistic significance( P < 0. 05). The expression of Snail1( 54. 2%) in colon cancer tissues was significantly higher than that in adjacent normal tissues( 30%) with statistic significance( P < 0. 05). The expression of KLF8 and Snail1 was related with TNM staging of tumor,depth of invasion and lymph node metastasis( P < 0. 05),and no association was observed in age,gender,tumor size and histological grading( P > 0. 05). The two factors were uncorrelated in colon cancer tissues( r =-0. 194,P > 0. 05). Conclusion: KLF8 and Snail1 show high expression in colon cancer tissues. But there was no correlation between the two factors,suggesting that KLF8 and Snail1 may be involved in occurrence and development of colon cancer by different ways.
Objective: To investigate the expression and clinical significance of Krüppel like factor 8( KLF8) and Zinc finger protein transcription factor 1( Snail1) in colon cancer tissues. Methods: 70 cases of colon cancer and 30 adjacent normal tissues were collected. Immunohistochemical method was used to determine the expression of KLF8 and Snail1 in those tissues.The correlation of the two factors in colon cancer tissues,and the relationship of the two factors with pathological features were analyzed. Results: The expression of KLF8( 71. 4%) in colon cancer tissues was significantly higher than that in adjacent normal tissues( 20%) with statistic significance( P < 0. 05). The expression of Snail1( 54. 2%) in colon cancer tissues was significantly higher than that in adjacent normal tissues( 30%) with statistic significance( P < 0. 05). The expression of KLF8 and Snail1 was related with TNM staging of tumor,depth of invasion and lymph node metastasis( P < 0. 05),and no association was observed in age,gender,tumor size and histological grading( P > 0. 05). The two factors were uncorrelated in colon cancer tissues( r =-0. 194,P > 0. 05). Conclusion: KLF8 and Snail1 show high expression in colon cancer tissues. But there was no correlation between the two factors,suggesting that KLF8 and Snail1 may be involved in occurrence and development of colon cancer by different ways.
引文
[1]汤国辉,谢远杰,刘丽兵,等. ERK1/2与MMP2在结肠癌中的表达及意义〔J〕.诊断病理学杂志,2016,23(10):784-788.
[2] Acloque H,Thiery JP,Nieto MA. The physiology and pathology ofthe EMT. Meeting on the Epithelial-Mesenchymal Transition〔J〕.Embo Reports,2008,9(4):322-326.
[3] Chang JY,Wright JM,Svoboda KK. Signal transduction pathwaysinvolved in epithelial-mesenchymal transition in oral cancer com-pared with other cancers〔J〕. Cells Tissues Organs,2007,185(1-3):40-47.
[4] Fang JH,Zhou HC,Zhang C,et al. A novel vascular pattern pro-motes metastasis of hepatocellular carcinoma in an EMT-independ-ent manner〔J〕. Hepatology,2015,62(2):452-465.
[5]王鑫,李峰,谭家明. EMT与肿瘤侵袭转移的研究进展〔J〕.蛇志,2016,28(3):342-345.
[6] Zhang H,Liu L,Wang Y,et al. KLF8 involves in TGF-beta-inducedEMT and promotes invasion and migration in gastric cancer cells〔J〕. Journal of Cancer Research&Clinical Oncology,2013,139(6):1033-1042.
[7] Mikami S,Katsube K,Oya M,et al. Expression of Snail and Slug inrenal cell carcinoma:E-cadherin repressor Snail is associated withcancer invasion and prognosis〔J〕. Laboratory investigation; a jour-nal of technical methods and pathology,2011,91(10):1443.
[8] Wang X,Zheng M,Liu G,et al. Krüppel-like factor 8 induces epi-thelial to mesenchymal transition and epithelial cell invasion〔J〕.Cancer Research,2007,67(15):7184-7193.
[9]王鑫,李峰,谭家明. EMT与肿瘤侵袭转移的研究进展〔J〕.蛇志,2016,28(3):342-345.
[10]刘卉芳,曹梦婷,徐薇,等.调控肿瘤EMT的转录因子及其相关信号通路〔J〕.现代肿瘤医学,2017,25(13):2155-2160.
[11]万伟峰,朱继,方升,等. Krüppel样转录因子8(KLF8)的结构及功能〔J〕.中国生物化学与分子生物学报,2011,27(4):305.
[12]鲍子戌,周海胜.肿瘤细胞的EMT与免疫逃逸的研究进展〔J〕.生命的化学,2017,37(6):980-985.
[13] Peinado H,Portillo F,Cano A. Transcriptional regulation of cad-herins during development and carcinogenesis〔J〕. InternationalJournal of Developmental Biology,2004,48(5-6):365-375.
[14]许明召,刘宏旭,崔贵. Kruppel样转录因子8的结构和功能多样性〔J〕.中国医药导报,2015,12(12):29-31.
[15]徐嘉威,贺花,张静,等.转录因子KLF8基因结构及其功能研究进展〔J〕.中国生物工程杂志,2018,43(4):90-95.
[16] Yi XH,Wang J. Expression of transcription factor Klf8 in lungcancer tissue and the biological effect of downregulation of Klf8expression in lung cancer cell lines〔J〕.海南医科大学学报:英文版,2016,22(2):9-12.
[17]成撒诺,李甲初,张幸平. Krüpple样转录因子8在恶性肿瘤中的研究进展〔J〕.重庆医学,2015,44(13):1849-1851.
[18]李天梁,徐亮,李蜀华,等. Kruppel样因子8通过转化生长因子-β1介导的上皮间质转化促进胃癌细胞的侵袭转移〔J〕.中国老年学杂志,2018,38(9):2214-2218.
[19] Qiao M,Sheng S,Pardee AB. Taylor&Francis Online:Metastasisand AKT activation-Cell Cycle-Volume 7,Issue 19〔J〕. Taylor&Francis,2008,7(19):2991-2996.
[20]孙辉,孙文静,杨正凯,等. Snail1基因启动子区组蛋白修饰在前列腺癌细胞EMT发生中的作用〔J〕.哈尔滨医科大学学报,2016,50(5):397-402.
[21]曹广鑫,张一心,王小林.膀胱癌上皮间质化相关基因的表达研究〔J〕.江苏医药,2014,40(17):2061-2062.
[22]余红,叶贤伟,万自芬,等. Snaill和MMP-1在非小细胞肺癌表达的临床意义〔J〕.重庆医学,2012,41(28):2919-2921.
[2] Acloque H,Thiery JP,Nieto MA. The physiology and pathology ofthe EMT. Meeting on the Epithelial-Mesenchymal Transition〔J〕.Embo Reports,2008,9(4):322-326.
[3] Chang JY,Wright JM,Svoboda KK. Signal transduction pathwaysinvolved in epithelial-mesenchymal transition in oral cancer com-pared with other cancers〔J〕. Cells Tissues Organs,2007,185(1-3):40-47.
[4] Fang JH,Zhou HC,Zhang C,et al. A novel vascular pattern pro-motes metastasis of hepatocellular carcinoma in an EMT-independ-ent manner〔J〕. Hepatology,2015,62(2):452-465.
[5]王鑫,李峰,谭家明. EMT与肿瘤侵袭转移的研究进展〔J〕.蛇志,2016,28(3):342-345.
[6] Zhang H,Liu L,Wang Y,et al. KLF8 involves in TGF-beta-inducedEMT and promotes invasion and migration in gastric cancer cells〔J〕. Journal of Cancer Research&Clinical Oncology,2013,139(6):1033-1042.
[7] Mikami S,Katsube K,Oya M,et al. Expression of Snail and Slug inrenal cell carcinoma:E-cadherin repressor Snail is associated withcancer invasion and prognosis〔J〕. Laboratory investigation; a jour-nal of technical methods and pathology,2011,91(10):1443.
[8] Wang X,Zheng M,Liu G,et al. Krüppel-like factor 8 induces epi-thelial to mesenchymal transition and epithelial cell invasion〔J〕.Cancer Research,2007,67(15):7184-7193.
[9]王鑫,李峰,谭家明. EMT与肿瘤侵袭转移的研究进展〔J〕.蛇志,2016,28(3):342-345.
[10]刘卉芳,曹梦婷,徐薇,等.调控肿瘤EMT的转录因子及其相关信号通路〔J〕.现代肿瘤医学,2017,25(13):2155-2160.
[11]万伟峰,朱继,方升,等. Krüppel样转录因子8(KLF8)的结构及功能〔J〕.中国生物化学与分子生物学报,2011,27(4):305.
[12]鲍子戌,周海胜.肿瘤细胞的EMT与免疫逃逸的研究进展〔J〕.生命的化学,2017,37(6):980-985.
[13] Peinado H,Portillo F,Cano A. Transcriptional regulation of cad-herins during development and carcinogenesis〔J〕. InternationalJournal of Developmental Biology,2004,48(5-6):365-375.
[14]许明召,刘宏旭,崔贵. Kruppel样转录因子8的结构和功能多样性〔J〕.中国医药导报,2015,12(12):29-31.
[15]徐嘉威,贺花,张静,等.转录因子KLF8基因结构及其功能研究进展〔J〕.中国生物工程杂志,2018,43(4):90-95.
[16] Yi XH,Wang J. Expression of transcription factor Klf8 in lungcancer tissue and the biological effect of downregulation of Klf8expression in lung cancer cell lines〔J〕.海南医科大学学报:英文版,2016,22(2):9-12.
[17]成撒诺,李甲初,张幸平. Krüpple样转录因子8在恶性肿瘤中的研究进展〔J〕.重庆医学,2015,44(13):1849-1851.
[18]李天梁,徐亮,李蜀华,等. Kruppel样因子8通过转化生长因子-β1介导的上皮间质转化促进胃癌细胞的侵袭转移〔J〕.中国老年学杂志,2018,38(9):2214-2218.
[19] Qiao M,Sheng S,Pardee AB. Taylor&Francis Online:Metastasisand AKT activation-Cell Cycle-Volume 7,Issue 19〔J〕. Taylor&Francis,2008,7(19):2991-2996.
[20]孙辉,孙文静,杨正凯,等. Snail1基因启动子区组蛋白修饰在前列腺癌细胞EMT发生中的作用〔J〕.哈尔滨医科大学学报,2016,50(5):397-402.
[21]曹广鑫,张一心,王小林.膀胱癌上皮间质化相关基因的表达研究〔J〕.江苏医药,2014,40(17):2061-2062.
[22]余红,叶贤伟,万自芬,等. Snaill和MMP-1在非小细胞肺癌表达的临床意义〔J〕.重庆医学,2012,41(28):2919-2921.