葛根芩连汤及其主要成分对L6骨骼肌细胞糖代谢的调节作用
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摘要
目的建立地塞米松(DEX)诱导的L6骨骼肌细胞胰岛素抵抗模型,探讨葛根芩连汤及其主要成分(葛根素、小檗碱、黄芩苷)对L6骨骼肌细胞胰岛素抵抗模型葡萄糖消耗量的影响。方法将诱导分化好的L6肌细胞按106个·ml-1接种于96孔板内,以MTT法检测葛根芩连汤含药血清对L6肌细胞增殖的影响;以1μmol/L DEX诱导L6肌细胞24 h产生胰岛素抵抗,予以不同浓度的葛根芩连汤含药血清及其主要成分(葛根素、小檗碱、黄芩苷)进行干预,100 nmol/L胰岛素刺激30min,采用葡萄糖氧化酶法检测L6肌细胞的葡萄糖消耗量。结果葛根芩连汤含药血清对L6肌细胞增殖无显著影响。与正常组比较,模型组葡萄糖消耗量明显下降;葛根芩连汤含药血清(5%、10%、15%)、葛根素(1,10,100μmol·L-1)、小檗碱(1μmol/L)和黄芩苷(1,10,100μmol·L-1)能显著增加L6骨骼肌细胞胰岛素抵抗模型葡萄糖消耗量(P<0.05)。结论葛根芩连汤及其主要成分(葛根素、小檗碱、黄芩苷)可调节L6骨骼肌细胞胰岛素抵抗模型糖代谢,进而可改善肌细胞胰岛素抵抗。
Objective To investigate the effects of Gegen Qinlian Decoction- containing serum( GGQLD- CS) and its active ingredients( puerarin,berberine,baicalin) on glycometabolism in insulin- resistance L6 Cell Line. Methods GGQLD- CS was prepared. MTT was used to detect the effects of GGQLD- CS on cell proliferation; establishing the model of insulin resistance,studying the effect of GGQLD- CS and its active ingredients( puerarin,berberine,baicalin) on the glucose consumption of L6 cells in insulin resistance by the method of glucose oxidase. Results GGQLD- CS and its active ingredients could promote glucose consumption of L6 cell in insulin resistance. Conclusion GGQLD- CS and its active ingredients can regulate glucose metabolism of L6 cells,and improved L6 cells insulin resistance.
Objective To investigate the effects of Gegen Qinlian Decoction- containing serum( GGQLD- CS) and its active ingredients( puerarin,berberine,baicalin) on glycometabolism in insulin- resistance L6 Cell Line. Methods GGQLD- CS was prepared. MTT was used to detect the effects of GGQLD- CS on cell proliferation; establishing the model of insulin resistance,studying the effect of GGQLD- CS and its active ingredients( puerarin,berberine,baicalin) on the glucose consumption of L6 cells in insulin resistance by the method of glucose oxidase. Results GGQLD- CS and its active ingredients could promote glucose consumption of L6 cell in insulin resistance. Conclusion GGQLD- CS and its active ingredients can regulate glucose metabolism of L6 cells,and improved L6 cells insulin resistance.
引文
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[11]张召锋,丁叶,戴小倩,等.地塞米松诱导L6肌细胞胰岛素抵抗模型的建立及EGCG的保护作用研究[J].中国预防医学杂志,2009,10(12):1042.
[12]杨亮,迟戈,张俊,等.L6细胞胰岛素抵抗的骨骼肌细胞模型[J].中国组织工程研究与临床康复,2009,13(2):248.
[13]Zhang ZF,Li Q,Liang J,et al.Epigallocatechin-3-O-gallate(EGCG)protects the insulin sensitivity in rat L6 muscle cells exposed to dexamethasone condition[J].Phytomedicine.2010,17(1):14.
[14]Kong WJ,Zhang H,Song DQ,et al.Berberine reduces insulin resistance through protein kinase C-dependent up-regulation of insulin receptor expression[J].Metabolism,2009,58(1):109.
[2]Tong XL,Zhao LH,Lian FM,et al.Clinical observations on the dose-effect relationship of gegen qin lian decoction on 54 out-patients with type 2 diabetes[J].J Tradit Chin Med,2011,31(1):56.
[3]Zhang CH,Xu GL,Liu YH,et al.Anti-diabetic activities of Gegen Qinlian Decoction in high-fat diet combined with streptozotocin-induced diabetic rats and in 3T3-L1 adipocytes[J].Phytomedicine,2013,20(3-4):221.
[4]Li H,Zhao L,Zhang B,et al.A network pharmacology approach to determine active compounds and action mechanisms of ge-gen-qinlian decoction for treatment of type 2 diabetes[J].Evid Based Complement Alternat Med,2014,2014:495840.
[5]Zhang X,Zhao Y,Zhang M,et al.Structural changes of gut microbiota during berberine-mediated prevention of obesity and insulin resistance in high-fat diet-fed rats[J].PLo S One,2012,7(8):e42529.
[6]Zhang W,Liu CQ,Wang PW,et al.Puerarin improves insulin resistance and modulates adipokine expression in rats fed a high-fat diet[J].Eur J Pharmacol,2010,649(1-3):398.
[7]刘长山,王秀军,孙丽萍,等.黄芩苷对2型糖尿病大鼠模型胰岛素抵抗的影响[J].中国老年学杂志,2010,30(23):3541.
[8]Zhang CH,Yu RY,Liu YH,et al.Interaction of baicalin with berberine for glucose uptake in 3T3-L1 adipocytes and Hep G2 hepatocytes[J].J Ethnopharmacol,2014,151(2):864.
[9]盛军庆,章常华,涂秀英,等.葛根素对Hep G2细胞胰岛素抵抗状态的改善作用[J].时珍国医国药,2014,25(8):1831.
[10]章常华,邓可众,于梅,等.葛根芩连汤含药血清对Hep G2肝细胞胰岛素抵抗模型糖代谢的调节作用[J].中国实验方剂学杂志,2015,21(5):120.
[11]张召锋,丁叶,戴小倩,等.地塞米松诱导L6肌细胞胰岛素抵抗模型的建立及EGCG的保护作用研究[J].中国预防医学杂志,2009,10(12):1042.
[12]杨亮,迟戈,张俊,等.L6细胞胰岛素抵抗的骨骼肌细胞模型[J].中国组织工程研究与临床康复,2009,13(2):248.
[13]Zhang ZF,Li Q,Liang J,et al.Epigallocatechin-3-O-gallate(EGCG)protects the insulin sensitivity in rat L6 muscle cells exposed to dexamethasone condition[J].Phytomedicine.2010,17(1):14.
[14]Kong WJ,Zhang H,Song DQ,et al.Berberine reduces insulin resistance through protein kinase C-dependent up-regulation of insulin receptor expression[J].Metabolism,2009,58(1):109.