Distribution and relationship between antimicrobial resistance genes and heavy metals in surface sediments of Taihu Lake,China
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摘要
Heavy metals, pharmaceuticals, and other wastes released into the environment can significantly influence environmental antibiotic resistance. We investigated the occurrence of 22 antimicrobial resistance genes(ARGs) and 10 heavy metal concentrations, and the relationship between ARGs and heavy metals in surface sediment from seven sites of Lake Taihu. The results showed significant correlations(p < 0.05) between sediment ARG levels,especially for tetracycline and sulfonamides(e.g., tet(A), tet(D), tet(E), tet(O), sul1, sul2 and int-1) and specific heavy metals(Fe, Mn, Cr, Cu, Zn, among others) in the Lake. In the surface sediments, heavy metals showed an interaction with resistance genes, but the strength of interaction was diminished with increasing depth. For most of the heavy metals, the concentration of elements in the top sediments was higher than that in other depths.Tetracycline resistance genes(tet(A), tet(B), tet(D), tet(E) and tet(O), β-lactam resistance genes(SHV, TEM, CTX, OXA and OXY) and sulfonamide resistance genes(sulA, sul1, sul2, sul3 and int-1) were detected. They showed a trend which inferred a statistically significant increase followed by decreases in the relative abundance of these ARGs(normalized to 16 S rRNA genes) with increasing depth. This study revealed that tet(A), tet(O), TEM, OXY, int-1, sul1 and sul3 were widespread in surface sediments with high abundance, indicating that these genes deserve more attention in future work.
Heavy metals, pharmaceuticals, and other wastes released into the environment can significantly influence environmental antibiotic resistance. We investigated the occurrence of 22 antimicrobial resistance genes(ARGs) and 10 heavy metal concentrations, and the relationship between ARGs and heavy metals in surface sediment from seven sites of Lake Taihu. The results showed significant correlations(p < 0.05) between sediment ARG levels,especially for tetracycline and sulfonamides(e.g., tet(A), tet(D), tet(E), tet(O), sul1, sul2 and int-1) and specific heavy metals(Fe, Mn, Cr, Cu, Zn, among others) in the Lake. In the surface sediments, heavy metals showed an interaction with resistance genes, but the strength of interaction was diminished with increasing depth. For most of the heavy metals, the concentration of elements in the top sediments was higher than that in other depths.Tetracycline resistance genes(tet(A), tet(B), tet(D), tet(E) and tet(O), β-lactam resistance genes(SHV, TEM, CTX, OXA and OXY) and sulfonamide resistance genes(sulA, sul1, sul2, sul3 and int-1) were detected. They showed a trend which inferred a statistically significant increase followed by decreases in the relative abundance of these ARGs(normalized to 16 S rRNA genes) with increasing depth. This study revealed that tet(A), tet(O), TEM, OXY, int-1, sul1 and sul3 were widespread in surface sediments with high abundance, indicating that these genes deserve more attention in future work.
Heavy metals, pharmaceuticals, and other wastes released into the environment can significantly influence environmental antibiotic resistance. We investigated the occurrence of 22 antimicrobial resistance genes(ARGs) and 10 heavy metal concentrations, and the relationship between ARGs and heavy metals in surface sediment from seven sites of Lake Taihu. The results showed significant correlations(p < 0.05) between sediment ARG levels,especially for tetracycline and sulfonamides(e.g., tet(A), tet(D), tet(E), tet(O), sul1, sul2 and int-1) and specific heavy metals(Fe, Mn, Cr, Cu, Zn, among others) in the Lake. In the surface sediments, heavy metals showed an interaction with resistance genes, but the strength of interaction was diminished with increasing depth. For most of the heavy metals, the concentration of elements in the top sediments was higher than that in other depths.Tetracycline resistance genes(tet(A), tet(B), tet(D), tet(E) and tet(O), β-lactam resistance genes(SHV, TEM, CTX, OXA and OXY) and sulfonamide resistance genes(sulA, sul1, sul2, sul3 and int-1) were detected. They showed a trend which inferred a statistically significant increase followed by decreases in the relative abundance of these ARGs(normalized to 16 S rRNA genes) with increasing depth. This study revealed that tet(A), tet(O), TEM, OXY, int-1, sul1 and sul3 were widespread in surface sediments with high abundance, indicating that these genes deserve more attention in future work.
引文
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Asha,R.C.,Yadav,M.S.P.,Kumar,M.,2018.Sulfamethoxazole removal in membrane-photocatalytic reactor system-experimentation and modelling.Environ.Technol.42,1-8.
Beukers,A.G.,Zaheer,R.,Cook,S.R.,Chaves,A.V.,Ward,M.P.,Tymensen,L.,et al.,2018.Comparison of antimicrobial resistance genes in feedlots and urban wastewater.Can.J.Vet.Res.82,24-38.
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Burgess,R.M.,Berry,W.J.,Mount,D.R.,Di Toro,D.M.,2013.Mechanistic sediment quality guidelines based on contaminant bioavailability:Equilibrium partitioning sediment benchmarks.Environ.Toxicol.Chem.32(1),102-114.
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Chen,D.Q.,Jiang,Y.T.,Feng,D.H.,Wen,S.X.,Su,D.H.,Yang,L.,2018.Integron mediated bacterial resistance and virulence on clinical pathogens.Microb.Pathog.114,453-457.
Chenia,H.,Jacobs,A.,2017.Antimicrobial resistance,heavy metal resistance and integron content in bacteria isolated from a south African tilapia aquaculture system.Dis.Aquat.Org.126,199-209.
Cordova-Kreylos,A.L.,Scow,K.M.,2007.Effects of ciprofloxacin on salt marsh sediment microbial communities.ISME J.1,585-595.
Deng,X.,Xu,Y.,Han,L.,Song,S.,Yang,L.,Li,G.,et al.,2015.Impacts of urbanization on river systems in the Taihu Region,China.Water 7,1340-1358.
El-Razik,K.A.A.,Arafa,A.A.,Hedia,R.H.,Ibrahim,E.S.,2017.Tetracycline resistance phenotypes and genotypes of coagulase-negative staphylococcal isolates from bubaline mastitis in Egypt.Vet.World.10,702-710.
Feng,X.,Foucher,D.,Hintelmann,H.,Yan,H.,He,T.,Qiu,G.,2010.Tracing mercury contamination sources in sediments using mercury isotope compositions.Environ.Sci.Technol.44,3363-3368.
Garbisu,C.,Garaiyurrebaso,O.,Lanzén,A.,álvarez-Rodríguez,I.,Arana,L.,Blanco,F.,et al.,2018.Mobile genetic elements and antibiotic resistance in mine soil amended with organic wastes.Sci.Total Environ.621,725-733.
Gayatri,A.A.A.Y.,Utama,S.,Somia,A.,Merati,T.P.,2015.MRSAinfection in patients hospitalized at Sanglah hospital:a case series.Acta Med.Indones.47,50-55.
Graham,D.W.,Olivares-Rieumont,S.,Knapp,C.W.,Lima,L.,Werner,D.,Bowen,E.,2010.Antibiotic resistance gene abundances associated with waste discharges to the Almendares River near Havana,Cuba.Environ.Sci.Technol.45,418-424.
Guo,X.,Yan,Z.,Zhang,Y.,Xu,W.,Kong,D.,Shan,Z.,et al.,2018.Behavior of antibiotic resistance genes under extremely highlevel antibiotic selection pressures in pharmaceutical wastewater treatment plants.Sci.Total Environ.612,119-128.
Han,J.,Pendleton,S.J.,Deck,J.,Singh,R.,Gilbert,J.,Johnson,T.J.,et al.,2018.Impact of co-carriage of IncA/C plasmids with additional plasmids on the transfer of antimicrobial resistance in Salmonella enterica isolates.Int.J.Food Microbiol.271,77-84.
Harnisz,M.,Korzeniewska,E.,2018.The prevalence of multidrugresistant Aeromonas spp.in the municipal wastewater system and their dissemination in the environment.Sci.Total Environ.626,377-383.
Harnisz,M.,Korzeniewska,E.,Go?a?,I.,2015.The impact of a freshwater fish farm on the community of tetracyclineresistant bacteria and the structure of tetracycline resistance genes in river water.Chemosphere 128,134-141.
He,X.,Xu,Y.,Chen,J.,Ling,J.,Li,Y.,Huang,L.,et al.,2017.Evolution of corresponding resistance genes in the water of fish tanks with multiple stresses of antibiotics and heavy metals.Water Res.124,39-48.
Heuer,H.,Focks,A.,Lamsh?ft,M.,Smalla,K.,Matthies,M.,Spiteller,M.,2008.Fate of sulfadiazine administered to pigs and its quantitative effect on the dynamics of bacterial resistance genes in manure and manured soil.Soil Biol.Biochem.40,1892-1900.
Heuer,H.,Kopmann,C.,Binh,C.T.T.,Top,E.M.,Smalla,K.,2009.Spreading antibiotic resistance through spread manure:characteristics of a novel plasmid type with low%G+C content.Environ.Microbiol.11,937-949.
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