一株驯化血芝的基源鉴定
|
摘要
龙门马岭灵芝种植基地成功将野生血芝驯化栽培,为了鉴定该驯化种的基源,从基地采摘了一株驯化血芝,分别通过观察样品的形态、大小、颜色等性状特征进行性状鉴定,观察菌管、菌丝、孢子等显微特征进行显微鉴定,采用内部转录间隔区(internal transcriptional spacer,ITS)序列分析手段进行分子鉴定。结果表明,样品的性状特征,及其菌管、菌丝、孢子的显微特征与《中国真菌志》中假芝属假芝(Amauroderma rugosum)特征描述相符;ITS序列分析表明样品与假芝属假芝(A.rugosum)的同源性为99%。因此,经鉴定该驯化血芝为灵芝科假芝属假芝(A. rugosum)。
A wild Amauroderma mushroom was domesticated successfully in Longmen Maling ganoderma cultivation base. In order to identify the origin,a strain of Amauroderma mushroom was collected from the base and identified by morphological,microscopic and molecular identifications. Macroscopic identification was carried out by observing the morphology,size and color of the sample,and microscopic identification was carried out by observing the microscopic characteristics of the mediostratum,mycelium and spore,and molecular identification was performed by internal transcriptional spacer(ITS)sequence analysis. The experiment result showed that the morphological and microscopic characteristics were consistent with those of A. rugosum in the"Chinese Mycology";the ITS sequence analysis showed that the similarity value between the sample and A. rugosum was 99%. So this domesticated Amauroderma mushroom is identified as A. rugosum.
A wild Amauroderma mushroom was domesticated successfully in Longmen Maling ganoderma cultivation base. In order to identify the origin,a strain of Amauroderma mushroom was collected from the base and identified by morphological,microscopic and molecular identifications. Macroscopic identification was carried out by observing the morphology,size and color of the sample,and microscopic identification was carried out by observing the microscopic characteristics of the mediostratum,mycelium and spore,and molecular identification was performed by internal transcriptional spacer(ITS)sequence analysis. The experiment result showed that the morphological and microscopic characteristics were consistent with those of A. rugosum in the"Chinese Mycology";the ITS sequence analysis showed that the similarity value between the sample and A. rugosum was 99%. So this domesticated Amauroderma mushroom is identified as A. rugosum.
引文
[1]Dai Y C,Tuli G E,Cui B K.Medicinal fungi of China illustrations[M].Harbin:Northeast Forestry University Publishing House,2013:38-41.戴玉成,图力吉尔,崔宝凯.中国药用真菌图志[M].哈尔滨:东北林业大学出版社,2013:38-41.
[2]Chen T Q,Li K B.Resources,taxonomy,ecological distribution,exploitation and utilization of Ganodermata-ceae from China[J].Acta Agric Univ Jiangxiensis Nat Sci Ed,2004,26(1):89-95.陈体强,李开本.中国灵芝科真菌资源分类、生态分布及其合理开发利用[J].江西农业大学学报,2004,26(1):89-95.
[3]Zhang H H,Wu B H.A preliminary study on the culti-vation technique of Amauroderma mushroom in north Guangdong[J].Special Econ Animal Plant,2014,17(5):44-45.张红红,巫宝花.粤北地区血芝袋料栽培技术初探[J].特种经济动植物,2014,17(5):44-45.
[4]Yue W,Huang T,Qiu R L,et al.Macroscopic and mi-croscopic identification of Ganoderma and the confus-able medicinal mushrooms of“Zhi”[J].J Chin Med Ma-ter,2015,38(11):2293-2297.乐巍,黄涛,邱蓉丽,等.灵芝及常见易混“芝”类药材的性状及显微鉴别研究[J].中药材,2015,38(11):2293-2297.
[5]Qin G J,Wang S S,Chen Q J,et al.Isolation,identifi-cation,and biological characteristics of a wild Amauroderma mushroom[J].Chin J Appl Environ Biol,2015,21(3):464-469.秦改娟,王珊珊,陈青君,等.一株假芝的分离鉴定与生物学特征[J].应用与环境生物学报,2015,21(3):464-469.
[6]Zhao J D,Zhang X Q.Chinese mycology(Ganoderma family)[M].Beijing:Science Press,2000:144-183.赵继鼎,张小青.中国真菌志(灵芝科)[M].北京:科学出版社,2000:144-183.
[7]Zhang Y F,Xie Y Z,Yang X B.Advance in research on function of Amauroderma[J].Edible Fungi,2018,40(2):1-4.张一帆,谢意珍,杨小兵.假芝功效研究进展[J].食用菌,2018,40(2):1-4.
[8]Jiao C W,Xie Y Z,Yang X L,et al.Anticancer activity of Amauroderma rude[J].Plos One,2013,8(6):e66504.
[9]Chan P M,Kanagasabapathy G,Tan Y S,et al.Amauroderma rugosum(Blume&T.Nees)Torrend:nutri-tional composition and antioxidant and potential anti-in-flammatory properties[J].Evid-Based Complementary Altern Med,2013,2013:1-10.
[10]Seng C K,Abdullah N,Aminudin N.Lipid-modulat-ing effect of black lingzhi medicinal mushroom,Amauroderma rugosum(Agaricomycetes),on oleate-induced human hepatocellular liver carcinoma cells[J].Int JMed Mushrooms,2017,19(12):1101-1111.
[11]Utomo C,Werner S,Niepold F,et al.Identification of Ganoderma,the causal agent of basal stem rot disease in oil palm using a molecular method[J].Mycopatholo-gia,2005,159(1):159-170.
[12]Zhou J L,Zhao R L.Advances on DNA barcoding in fungi[J].Microbiol China,2013,40(8):1468-1477.周均亮,赵瑞琳.真菌DNA条形码技术研究进展[J].微生物学通报,2013,40(8):1468-1477.
[13]Zheng H B,Ma Z G,Lu Z Z,et al.Identification and evaluation of main cultivated species of Pleurotus in Chi-na based on ITS sequence analysis[J].Mycosystema,2006,25(3):398-407.郑和斌,马志刚,吕作舟,等.基于ITS序列分析对我国主要栽培的侧耳品种的鉴定及评价[J].菌物学报,2006,25(3):398-407.
[14]Hou J,Lin X M,Jiang Y,et al.Molecular identifica-tion of suspected“Baiyangdu”strain based on internal transcribed spacer sequence analysis[J].Food Scie,2009,30(5):141-144.侯军,林晓民,江芸,等.基于ITS序列分析对疑似白羊肚菌株的分子鉴定[J].食品科学,2009,30(5):141-144.
[15]Huang L H,Yang X B,Zhang Z,et al.Identification of Ganoderma spawns based on ITS sequence analysis[J].Edible Fungi China,2010,29(1):55-57.黄龙花,杨小兵,张智,等.基于ITS序列分析鉴定灵芝属菌种[J].中国食用菌,2010,29(1):55-57.
[2]Chen T Q,Li K B.Resources,taxonomy,ecological distribution,exploitation and utilization of Ganodermata-ceae from China[J].Acta Agric Univ Jiangxiensis Nat Sci Ed,2004,26(1):89-95.陈体强,李开本.中国灵芝科真菌资源分类、生态分布及其合理开发利用[J].江西农业大学学报,2004,26(1):89-95.
[3]Zhang H H,Wu B H.A preliminary study on the culti-vation technique of Amauroderma mushroom in north Guangdong[J].Special Econ Animal Plant,2014,17(5):44-45.张红红,巫宝花.粤北地区血芝袋料栽培技术初探[J].特种经济动植物,2014,17(5):44-45.
[4]Yue W,Huang T,Qiu R L,et al.Macroscopic and mi-croscopic identification of Ganoderma and the confus-able medicinal mushrooms of“Zhi”[J].J Chin Med Ma-ter,2015,38(11):2293-2297.乐巍,黄涛,邱蓉丽,等.灵芝及常见易混“芝”类药材的性状及显微鉴别研究[J].中药材,2015,38(11):2293-2297.
[5]Qin G J,Wang S S,Chen Q J,et al.Isolation,identifi-cation,and biological characteristics of a wild Amauroderma mushroom[J].Chin J Appl Environ Biol,2015,21(3):464-469.秦改娟,王珊珊,陈青君,等.一株假芝的分离鉴定与生物学特征[J].应用与环境生物学报,2015,21(3):464-469.
[6]Zhao J D,Zhang X Q.Chinese mycology(Ganoderma family)[M].Beijing:Science Press,2000:144-183.赵继鼎,张小青.中国真菌志(灵芝科)[M].北京:科学出版社,2000:144-183.
[7]Zhang Y F,Xie Y Z,Yang X B.Advance in research on function of Amauroderma[J].Edible Fungi,2018,40(2):1-4.张一帆,谢意珍,杨小兵.假芝功效研究进展[J].食用菌,2018,40(2):1-4.
[8]Jiao C W,Xie Y Z,Yang X L,et al.Anticancer activity of Amauroderma rude[J].Plos One,2013,8(6):e66504.
[9]Chan P M,Kanagasabapathy G,Tan Y S,et al.Amauroderma rugosum(Blume&T.Nees)Torrend:nutri-tional composition and antioxidant and potential anti-in-flammatory properties[J].Evid-Based Complementary Altern Med,2013,2013:1-10.
[10]Seng C K,Abdullah N,Aminudin N.Lipid-modulat-ing effect of black lingzhi medicinal mushroom,Amauroderma rugosum(Agaricomycetes),on oleate-induced human hepatocellular liver carcinoma cells[J].Int JMed Mushrooms,2017,19(12):1101-1111.
[11]Utomo C,Werner S,Niepold F,et al.Identification of Ganoderma,the causal agent of basal stem rot disease in oil palm using a molecular method[J].Mycopatholo-gia,2005,159(1):159-170.
[12]Zhou J L,Zhao R L.Advances on DNA barcoding in fungi[J].Microbiol China,2013,40(8):1468-1477.周均亮,赵瑞琳.真菌DNA条形码技术研究进展[J].微生物学通报,2013,40(8):1468-1477.
[13]Zheng H B,Ma Z G,Lu Z Z,et al.Identification and evaluation of main cultivated species of Pleurotus in Chi-na based on ITS sequence analysis[J].Mycosystema,2006,25(3):398-407.郑和斌,马志刚,吕作舟,等.基于ITS序列分析对我国主要栽培的侧耳品种的鉴定及评价[J].菌物学报,2006,25(3):398-407.
[14]Hou J,Lin X M,Jiang Y,et al.Molecular identifica-tion of suspected“Baiyangdu”strain based on internal transcribed spacer sequence analysis[J].Food Scie,2009,30(5):141-144.侯军,林晓民,江芸,等.基于ITS序列分析对疑似白羊肚菌株的分子鉴定[J].食品科学,2009,30(5):141-144.
[15]Huang L H,Yang X B,Zhang Z,et al.Identification of Ganoderma spawns based on ITS sequence analysis[J].Edible Fungi China,2010,29(1):55-57.黄龙花,杨小兵,张智,等.基于ITS序列分析鉴定灵芝属菌种[J].中国食用菌,2010,29(1):55-57.