益智仁缩尿功效成分的研究
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摘要
探究了益智仁的缩尿功效,并对其有效成分进行了分离鉴定。通过建立小鼠多尿模型,对有效部位进行缩尿活性筛选,采用代谢笼收集小鼠前2,6h内总尿量,并分别对其尿重/体重进行计算。结果表明,75%乙醇提取物乙酸乙酯萃取物对小鼠排尿的抑制效果最为明显,该部分经MCI、ODS-D柱分离,得到4种单体化合物,包括2个脂肪酸。运用NMR等波谱方法,并参考相关文献,对所得化合物进行结构分析,分别鉴定为3-甲氧基-4羟基-二苯己烷、20-丙基-β-谷甾醇、油酸、亚油酸。3-甲氧基-4羟基-二苯己烷和20-丙基-β-谷甾醇为首次从益智仁中分离得到并证实具有缩尿作用。
This research focus on the efficiency of Alpinia Oxyphylla Fructus in urine-reducing and developing the method of separating and identifying its active components.Established a mice model with diuresis to validate the Alpinia Oxyphylla Fructus' different components and purified by varying measures in reducing urine.The total urine volume in the first 2 and 6 hours of the mice was collected by metabolic cage.The values of urine weight/body weight were calculated respectively.The result showed that 75% ethanol extract of ethyl acetate phase was the most sensitive part in urine-reducing.Four pure monomer compounds were further separate from this part by MCI and ODS-D columns,including two fatty acids.The structure of components were identified as(1)3-methoxy-4-Hydroxy-diphenylhexane,(2)20-propyl-β-sitosterol,(3)oleic acid and(4)linoleic acid respectively,by the methods of NMR and other spectroscopy technology.Compound 1 and Compound 2 were separate from Alpinia Oxyphylla Fructus and confirmed to have effects on urine-reducing for the first time.
This research focus on the efficiency of Alpinia Oxyphylla Fructus in urine-reducing and developing the method of separating and identifying its active components.Established a mice model with diuresis to validate the Alpinia Oxyphylla Fructus' different components and purified by varying measures in reducing urine.The total urine volume in the first 2 and 6 hours of the mice was collected by metabolic cage.The values of urine weight/body weight were calculated respectively.The result showed that 75% ethanol extract of ethyl acetate phase was the most sensitive part in urine-reducing.Four pure monomer compounds were further separate from this part by MCI and ODS-D columns,including two fatty acids.The structure of components were identified as(1)3-methoxy-4-Hydroxy-diphenylhexane,(2)20-propyl-β-sitosterol,(3)oleic acid and(4)linoleic acid respectively,by the methods of NMR and other spectroscopy technology.Compound 1 and Compound 2 were separate from Alpinia Oxyphylla Fructus and confirmed to have effects on urine-reducing for the first time.
引文
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[9]龚晓猛,李梦琪,吴珊珊,等.盐炙益智仁石油醚部位缩尿成分分离与药效学分析[J].中国实验方剂学杂志,2017(6):13-18.
[10]石绍淮,张晨宁,刘爱敬,等.益智仁化学成分的分离与鉴定[J].中国实验方剂学志,2013,19(17):97-100.
[11]李文兵.基于中医“标本兼治”研究益智仁盐炙“温肾缩尿”作用机理[D].成都:成都中医药大学,2013:31-33.
[12]刘楠,于新宇,赵红,等.益智仁化学成分研究[J].中草药,2009,40(1):29-32.
[13]张俊清,王勇,陈峰,等.益智的化学成分与药理作用研究进展[J].天然产物研究与开发,2013,25(2):280-287.
[2]李文兵,胡昌江,吴珊珊,等.益智仁盐炙对水负荷多尿模型大鼠缩尿作用的研究[J].中国实验方剂学杂志,2013,19(11):261-264.
[3]CHUN K S,KANG J Y,KIM O H,et al.Effects of yakuchinone A and yakuchinone B on the phorbol ester-induced expression of COX-2and iNOS and activation of NF-kappa B in mouse skin[J].Journal of Environmental Pathology Toxicology&Oncology Official Organ of the International Society for Environmental Toxicology&Cancer,2002,21(2):131.
[4]YU Xin-yu,AN Li-jia,WANG Yong-qi,et al.Neuroprotective effect of Alpinia oxyphylla Miq.fruits against glutamateinduced apoptosis in cortical neurons[J].Toxicology Letters,2003,144(2):205-212.
[5]YU Xin-yu,WANG Yong-qui,ZHAO Hong,et al.Neuroprotective effect of Alpinia oxyphylla extract against glutamate-induced apoptosis in cultured mouse cortical neurons[J].Neuroscience Research Communications,2003,33(2):105-103.
[6]黄凌,朱毅,董志,等.益智仁挥发油急性毒性实验及对帕金森小鼠行为学和纹状体多巴胺含量的影响[J].中药材,2008,31(5):722-726.
[7]黄勤挽.益智仁盐炙“缩尿”作用的研究[D].成都:成都中医药大学,2008:99-100.
[8]黄勤挽,胡昌江,李兴华,等.盐益智仁“缩尿”有效部位指纹图谱研究[J].中药与临床,2010,1(3):18-20.
[9]龚晓猛,李梦琪,吴珊珊,等.盐炙益智仁石油醚部位缩尿成分分离与药效学分析[J].中国实验方剂学杂志,2017(6):13-18.
[10]石绍淮,张晨宁,刘爱敬,等.益智仁化学成分的分离与鉴定[J].中国实验方剂学志,2013,19(17):97-100.
[11]李文兵.基于中医“标本兼治”研究益智仁盐炙“温肾缩尿”作用机理[D].成都:成都中医药大学,2013:31-33.
[12]刘楠,于新宇,赵红,等.益智仁化学成分研究[J].中草药,2009,40(1):29-32.
[13]张俊清,王勇,陈峰,等.益智的化学成分与药理作用研究进展[J].天然产物研究与开发,2013,25(2):280-287.