Orthotopic induction of CH157MN convexity and skull base meningiomas into nude mice using stereotactic surgery and MRI characterization
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摘要
Meningioma in vivo research is hampered by the difficulty of establishing an easy and reproducible orthotopic model able to mimic the characteristics of a human meningioma. Moreover, leptomeningeal dissemination and high mortality are often associated with such orthotopical models, making them useless for clinical translation studies. An optimized method for inducing meningiomas in nude mice at two different sites is described in this paper and the high reproducibility and low mortality of the models are demonstrated. Skull base meningiomas were induced in the auditory meatus and convexity meningiomas were induced on the brain surface of 23 and 24 nude mice, respectively. Both models led to the development of a mass easily observable by imaging methods. Dynamic contrast enhanced MRI was used as a tool to monitor and characterize the pathology onset and progression. At the end of the study, histology was performed to confirm the neoplastic origin of the diseased mass.
Meningioma in vivo research is hampered by the difficulty of establishing an easy and reproducible orthotopic model able to mimic the characteristics of a human meningioma. Moreover, leptomeningeal dissemination and high mortality are often associated with such orthotopical models, making them useless for clinical translation studies. An optimized method for inducing meningiomas in nude mice at two different sites is described in this paper and the high reproducibility and low mortality of the models are demonstrated. Skull base meningiomas were induced in the auditory meatus and convexity meningiomas were induced on the brain surface of 23 and 24 nude mice, respectively. Both models led to the development of a mass easily observable by imaging methods. Dynamic contrast enhanced MRI was used as a tool to monitor and characterize the pathology onset and progression. At the end of the study, histology was performed to confirm the neoplastic origin of the diseased mass.
Meningioma in vivo research is hampered by the difficulty of establishing an easy and reproducible orthotopic model able to mimic the characteristics of a human meningioma. Moreover, leptomeningeal dissemination and high mortality are often associated with such orthotopical models, making them useless for clinical translation studies. An optimized method for inducing meningiomas in nude mice at two different sites is described in this paper and the high reproducibility and low mortality of the models are demonstrated. Skull base meningiomas were induced in the auditory meatus and convexity meningiomas were induced on the brain surface of 23 and 24 nude mice, respectively. Both models led to the development of a mass easily observable by imaging methods. Dynamic contrast enhanced MRI was used as a tool to monitor and characterize the pathology onset and progression. At the end of the study, histology was performed to confirm the neoplastic origin of the diseased mass.
引文
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2.van Furth WR,Laughlin S,Taylor MD,et al.Imaging of murine brain tumors using a 1.5 Tesla clinical MRI system.Can J Neurol Sci.2003;30:326-332.
3.Tsai JC,Goldman CK,Gillespie GY.Vascular endothelial growth factor in human glioma cell lines:induced secretion by EGF,PDGF-BB,and b FGF.J Neurosurg.1995;82:864-873.
4.Ragel BT,Jensen RL,Gillespie DL,Prescott SM,Couldwell WT.Celecoxib inhibits meningioma tumor growth in a mouse xenograft model.Cancer.2007;109:588-597.
5.Ragel BT,Elam IL,Gillespie DL,et al.A novel model of intracranial meningioma in mice using luciferase-expressing meningioma cells.JNeurosurg.2008;108:304-310.
6.Gogigeni VR,Nalla AK,Gupta R,Dinh DH,Klopfenstein JD,Rao JS.Chk2-mediated G2/M cell cycle arrest maintains radiation resistance in malignant meningioma cells.Cancer Lett.2011;313:64-75.
7.Karsy M,Hoang N,Barth T,et al.Combined hydroxyurea and verapamil in the clinical treatment of refractory meningioma:human and orthotopic xenograft studies.World Neurosurg.2016;86:210-219.
8.Campbell BA,Jhamb A,Maguire JA,Toyota B,Ma R.Meningiomas in2009:controversies and future challenges.Am J Clin Oncol.2009;32:73-85.