Lin28B/let-7信号通路参与胰腺癌化疗药物耐药性机制的实验研究
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摘要
目的通过靶向调控Lin28B/let-7基因信号通路,进一步证实Lin28B/let-7之间相互作用的分子机制及其与胰腺癌化疗耐药性的关系,探讨调控Lin28B/let-7通路改变胰腺癌化疗耐药性的可能性。方法将胰腺癌AsPC-1细胞分为基因过表达组、基因沉默组和空白对照组,其中基因过表达组将构建的过表达Lin28B质粒(pCMV6-Lin28B)转染至胰腺癌细胞中,基因沉默组转染沉默Lin28B质粒(pGFP-shLin28B)。采用Western blotting技术检测Lin28B蛋白表达情况,实时荧光定量PCR检测Lin28B、let-7家族mRNA表达水平。将转染干预后的两组细胞再用含有不同浓度吉西他滨的培养基继续孵育72h后,以MTS法检测其细胞活力。结果转染pCMV6-Lin28B质粒能使Lin28BmRNA及蛋白有效过表达,let-7家族表达水平下调;而pGFP-shLin28B质粒使Lin28BmRNA及蛋白表达水平降低,let-7家族表达水平升高。与之相对应的是,调控Lin28B_(high)/let-7_(low)显著提升了胰腺癌细胞对吉西他滨的耐受力,而调控Lin28B_(low)/let-7_(high)则使胰腺癌细胞对吉西他滨的敏感性增加。结论胰腺癌细胞中Lin28B基因可能通过靶向调节let-7表达水平使癌细胞对化疗药物产生耐药性,而调控Lin28B/let-7信号通路可能在逆转胰腺癌对吉西他滨化疗药物耐药性方面具有深入研究价值。
Objective To confirm the molecular mechanism and the relationship between Lin28B/let-7 pathway with pancreatic cancer chemotherapy resistance by targeting. Furthermore,to investigate the effect of changing Lin28B/let-7 pathway on gemcitabine resistance of human pancreatic cancer cells. Methods Pancreatic Aspc-1 cells were divided into overexpression group,silencing group and control.By transient transfectiontechnology,Overexpression:pCMV6-Lin28B plasmids and pCMV6. silencing: pGFP-shLin28B and pGFP were respectively transfected intopancreaticAsPC-1cells.Western blotting analysis Lin28B protein,realtime fluorescence quantitative PCR identified Lin28B mRNA and let-7 family microRNA. Finally,the transfection of AsPC-1Cells were treated with gemcitabine at the indicated concentrations.After 72 h of incubation,cell viability was measured by MTS assay to reflect the drug resistance.Results pCMV6-Lin28B plasmid enables to Lin28B protein effective overexpression,in contrast pGFP-shLin28B plasmid make Lin28B protein decreased. Meanwhile Let-7microRNA expression level there was a significant difference.Introduction of Lin28B_(high)/let-7_(low) increased the gemcitabine resistance. While Lin28B_(low)/let-7_(high) reduced it. Conclusion Lin28B targeting let-7 family microRNA may be involved in the induction of gemcitabine drug resistance of pancreatic cancer cells.
Objective To confirm the molecular mechanism and the relationship between Lin28B/let-7 pathway with pancreatic cancer chemotherapy resistance by targeting. Furthermore,to investigate the effect of changing Lin28B/let-7 pathway on gemcitabine resistance of human pancreatic cancer cells. Methods Pancreatic Aspc-1 cells were divided into overexpression group,silencing group and control.By transient transfectiontechnology,Overexpression:pCMV6-Lin28B plasmids and pCMV6. silencing: pGFP-shLin28B and pGFP were respectively transfected intopancreaticAsPC-1cells.Western blotting analysis Lin28B protein,realtime fluorescence quantitative PCR identified Lin28B mRNA and let-7 family microRNA. Finally,the transfection of AsPC-1Cells were treated with gemcitabine at the indicated concentrations.After 72 h of incubation,cell viability was measured by MTS assay to reflect the drug resistance.Results pCMV6-Lin28B plasmid enables to Lin28B protein effective overexpression,in contrast pGFP-shLin28B plasmid make Lin28B protein decreased. Meanwhile Let-7microRNA expression level there was a significant difference.Introduction of Lin28B_(high)/let-7_(low) increased the gemcitabine resistance. While Lin28B_(low)/let-7_(high) reduced it. Conclusion Lin28B targeting let-7 family microRNA may be involved in the induction of gemcitabine drug resistance of pancreatic cancer cells.
引文
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[4]Piskounnova E,Polytarrchou C,Thornton JE,et al.Lin28A and Lin28B inhibit let-7 micro RNA Biogenesis by distinct mechanisms[J].cell,2011,147(5):1066-1079.
[5]Shih Im,Wang TI,et al.Noteh signaling,gamma-secretase inhibitors,and cancer therapy[J].Cancer Res,2007,67(5):1879-1882.
[6]Masamune A,Shimosegawa T,et al.Signal transduction in pancreatic stellate cells[J].J.Gastroenterol,2009,44(4):249-260.
[7]Okada T,Motoi F,Kanno A,et al.Pancreatic intraepithelial neoplasia-3with localized acute pancreatitis in the main pancreatic duct[J].Clinical J Gastroenterology,2013,6(2):164-168.
[8]Zhou M,Zhao Y,Ding Y,et al.Warburg effect in chemosensitivity:Targeting lactate dehydrogenase A resensitizes taxolresistant cancer cells to taxol[J].Mol Cancer,2010,9:33-33.
[9]Ichiyoshi Y,Endo K,.Yamamoto,M,et al.Biological features determining the chemosensitivity of gastric cancer[J].Hum Cell,1995,8(4):157-61.
[10]Nam Y,Chen C,Gregory RI,et al.Molecular basis for interaction of let-7 micro RNA with Lin28[J].Cell,2011,147(5):1080-1091.
[11]Zhu W,Xu H,Zhu D,et al.mi R-200bc/429 cluster modulates multidrug resistance of human cancer cell lines by targeting BCL2 and XIAP[J].Cancer Chemother Pharmacol,2012,69(3):723-732.
[12]Pasquinelli AE,Reinhart BJ,Slack F,et al.Conservation of the sequence and temporal expression of let-7 heterochronic regulatory RNA[J].Nature,2000,408(6808):86.
[13]Takamizawa J,Konishi H,Yanagisawa K,et al.Reduced expression of the let-7 micro RNAs in human lung cancers in association with shortened postoperative survival[J].Cancer Res,2004,64(11):3753-3756.
[14]Johnson SM,Grosshans H,Shingara J,et al.RAS is regulated by the let-7 micro RNA family[J].Cell,2005,120(5):635-637.
[15]Kumar MS,Erkeland SJ,Pester RE,et al.Suppression of non-small cell lung tumor development by the let-7 micro RNA family[J].Proc Natl Acad Sci USA,2008,105(10):3903-3908.
[16]Trang P,Medina PP,Wiggins JF,et al.Regression of murine lung tumors by the let-7 micro RNA[J].Oncogene,2010,29(11):1580-1587.
[17]Shin H,Atsushi M,Shin M,et al.Mi R-365 induces gemcitabine resistance in pancreatic cancer cells by targeting the adaptor protein SHC1and pro-apoptotic regulator BAX[J].Cellular Signaling,2014,26(2):179-185.
[18]Tian N,Han Z,Li Z,et al.Lin28/let-7/Bcl-x L pathway:the underlying mechanism of drug resistance in Hep3B cells[J].Oncology Reports,2014,32(3):1050-1056.
[19]马超,丁月超,黄长山,等.人胰腺癌干细胞化疗耐药性及其微小RNA-200c的表达[J].中华实验外科杂志,2015,32,5.
[20]牛良波,张刚.Lin28b和C-myc在胰腺癌中的表达及其意义[D].遵义:遵义医学院,2012.