RP-HPLC-UV法同时测定毛大丁草中大丁苷和8-甲氧基补骨脂素的含量
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摘要
目的建立HPLC方法同时测定毛大丁草(Gerbera piloselloides(L.) Cass.)中大丁苷和8-甲氧基补骨脂素的含量。方法采用WondaCract ODS-2色谱柱(250 mm×4.6 mm,5μm),使用乙腈(A)-水(B)溶液为流动相,进行梯度洗脱,流速:1.0 mL·min~(-1),检测波长:282 nm,柱温:25℃。结果大丁苷和8-甲氧基补骨脂素的标准曲线线性关系良好,相关系数r分别为0.999 3和0.999 4。实验的精密度、重复性和稳定性均能达到要求,大丁苷的平均回收率为99.30%、8-甲氧基补骨脂素的平均回收率为99.40%。以上2种成分都可以与基线分离,并且无其他成分峰的干扰。结论本方法适用于毛大丁草药材的质量控制。
Objective To develop a method of high performance liquid chromatography for the content determination of Gerberinside and 8-Methoxypsoralen in Gerbera piloselloides(L.) Cass..Methods The analysis was performed on a WondaCract ODS-2 column(250 mm×4.6 mm,5 μm) with gradient elution.The mobile phase was water(B) and acetonitrile(A).The detection wavelength was 282 nm.The column temperature was set at 25 ℃.Results Gerberinside and 8-methoxypsoralen were separated well with a good linearity,the r values were 0.999 3 and 0.999 4,respectively.The precision,stability and repeatability of the experiment all meet the requirements.The average recovery rate of gerberinside was 99.30%,the average recovery rate of 8-methoxypsoralen was 99.40%.The above two composition could be separated from the baseline,and they had no interference from other component peaks.Conclusion The method has the advantages of simple steps,fast analytical speed and strong specificity,and can be used for the quality control of Gerbera piloselloides(L.) Cass..
Objective To develop a method of high performance liquid chromatography for the content determination of Gerberinside and 8-Methoxypsoralen in Gerbera piloselloides(L.) Cass..Methods The analysis was performed on a WondaCract ODS-2 column(250 mm×4.6 mm,5 μm) with gradient elution.The mobile phase was water(B) and acetonitrile(A).The detection wavelength was 282 nm.The column temperature was set at 25 ℃.Results Gerberinside and 8-methoxypsoralen were separated well with a good linearity,the r values were 0.999 3 and 0.999 4,respectively.The precision,stability and repeatability of the experiment all meet the requirements.The average recovery rate of gerberinside was 99.30%,the average recovery rate of 8-methoxypsoralen was 99.40%.The above two composition could be separated from the baseline,and they had no interference from other component peaks.Conclusion The method has the advantages of simple steps,fast analytical speed and strong specificity,and can be used for the quality control of Gerbera piloselloides(L.) Cass..
引文
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[17] 杨英来,杨涛,杨玉华,等.超高效液相色谱法测定浓缩当归丸和当归药材中光毒性化合物香豆素[J].分析化学研究简报,2013,41:1744-1748.
[2] 何可群,李相兴,杨琼.毛大丁草总黄酮含量测定[J].亚太传统医药,2013,9(3):36-38.
[3] 程用谦.中国植物志:第七十九卷[M].北京:科学出版社,1996:94.
[4] 郭美仙,胡亚婷,陆翠芬,等.毛大丁草根水煎剂对受试动物的镇咳、祛痰、平喘作用[J].大理学院学报,2013,12(6):1-4.
[5] 遵义医学院药理学组.毛大丁草镇咳、祛痰作用的实验研究[J].医药科技资料,1977(1):31-33.
[6] 肖瑛,李建北,丁怡.毛大丁草化学成分的研究[J].中草药,2003(2):17-19.
[7] 肖瑛,李建北,丁怡.毛大丁草根和根茎化学成分的研究[J].中国中药杂志,2002(8):37-39.
[8] LI J B,DING Y,XIAO Y,et al.Two novel dicoumaro-p-menthanes from gerbera piloselloides (L.) Cass.[J].Chemical and Pharmaceutical Bulletin,2004,52(11):1362-1364.
[9] LI J B,DING Y,WANG C,et al.Dibothrioclinin I and II,epimers from gerbera piloselloides (L.) Cass.[J].Acta Crystallogr C,2003,59(10):593-595.
[10] 张婷,王华华,车萌,等.基于反向找靶的抗肿瘤活性研究[J].计算机与应用化学,2016,33(3):353-358.
[11] 麻秀萍,谭静,蒋朝晖,等.HPLC测定毛大丁草中的熊果苷[J].华西药学杂志,2013,28(3):307-308.
[12] 王建军.广西靖西壮族端午药市植物地胆草和毛大丁草的研究[D].北京:中央民族大学,2013:36-47.
[13] 谷黎红,李铣,陈英杰,等.大丁草中抗菌活性成分的研究:人肠道微生物对大丁甙及其类似物的代谢产物[J].药学学报,1988(7):511-515.
[14] 冯玉书,施一鸣.大丁草及大丁甙抗菌作用的实验研究[J].沈阳药学院学报,1981(14):39-42.
[15] 郭秀芝,刘卫萍,杨杰.补骨脂的药理活性及其开发利用[J].中医药学报,2005(5):56-57.
[16] 国家医药管理局中草药情报中心站.植物药有效成分手册[M].北京:人民卫生出版社,1986:1155-1156.
[17] 杨英来,杨涛,杨玉华,等.超高效液相色谱法测定浓缩当归丸和当归药材中光毒性化合物香豆素[J].分析化学研究简报,2013,41:1744-1748.