人参、黄芪和刺五加提取物对斑蝥增效的作用及机制
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摘要
目的:观察人参、黄芪和刺五加提取物对斑蝥提取物抑制A549人肺腺癌细胞生长和促进肿瘤细胞凋亡作用的影响。方法:实验分为斑蝥提取物组、斑蝥提取物+人参组、斑蝥提取物+黄芪组、斑蝥提取物+刺五加组、斑蝥提取物+人参+黄芪+刺五加组和对照组(PBS缓冲液)。检测3种提取物分别以及联合应用对斑蝥提取物抑制A549人肺腺癌细胞的生长效应,A549细胞形态、细胞凋亡情况和Caspase-3凋亡相关蛋白表达的影响。结果:MTT比色法检测显示人参、黄芪和刺五加提取物能增强斑蝥提取物对A549细胞增殖的抑制作用,3种提取物联合使用对斑蝥提取物的增效作用更明显(P <0. 05)。镜下显示:斑蝥提取物能引起A549细胞悬浮、生长迟缓和活性降低;人参、黄芪和刺五加提取物干预后,细胞悬浮更加明显,死亡细胞数目也显著增多(P <0. 05)。流式检测显示:人参、黄芪和刺五加提取物能增强斑蝥提取物引起的A549细胞凋亡,3者联合使用对斑蝥提取物的增效作用更明显(P <0. 05)。Western blot法检测显示:人参、黄芪和刺五加提取物能一定程度上调斑蝥提取物所致A549细胞中Caspase-3蛋白的表达,3者联合使用后对斑蝥提取物的增效作用更明显(P <0. 05)。结论:人参、黄芪和刺五加提取物对斑蝥提取物具有一定的增效作用,3者联合使用增效作用更加明显,可能与调节A549细胞凋亡相关蛋白Caspase-3的表达相关。
Objective: To observe the effects of extracts of ginseng,Radix Astragali seu Hedysari and Radix et Caulis Acanthopanacis Senticosi on the growth inhibition and apoptosis of A549 human lung adenocarcinoma cells. Methods: The experiment was divided into Mylabris extract group,Mylabris extract + Ginseng group,Mylabris extract + Radix Astragali seu Hedysari group,Mylabris extract + Radix et Caulis Acanthopanacis Senticosi group,Mylabris extract + ginseng + Radix Astragali seu Hedysari + Radix et Caulis Acanthopanacis Senticosi group and control group( PBS buffer solution). The effects of three extracts on the growth inhibition of A549 human lung adenocarcinoma cells,the morphology of A549 cells,the apoptosis of A549 cells and the expression of Caspase-3 apoptosis-related proteins were detected. Results: MTT assay showed that the extracts of Ginseng,Radix Astragali seu Hedysari and Radix et Caulis Acanthopanacis Senticosi could enhance the inhibitory effect of Mylabris extracts on A549 cell proliferation,and the synergistic effect of three extracts on Mylabris extracts was more obvious( P < 0. 05). Microscopically,the extract of Mylabris could cause A549 cell suspension,growth retardation and activity decrease. After the intervention of Ginseng,stragali seu Hedysari and Radix et Caulis Acanthopanacis Senticosi,cell suspension was more obvious and the number of dead cells increased significantly( P < 0. 05). Flow pattern analysis showed that ginseng,Radix Astragali seu Hedysari and Radix et Caulis Acanthopanacis Senticosi extracts could enhance the apoptosis of A549 cells induced by Mylabris extracts,and the synergistic effect of the three extracts on Mylabris extracts was more obvious( P < 0. 05). Western blot analysis showed that the extracts of ginseng,Astragalu Radix Astragali seu Hedysari and Radix et Caulis Acanthopanacis Senticosi could regulate the expression of Caspase-3 protein in A549 cells induced by extracts of Mylabris to some extent,and the synergistic effect of the three extracts on Mylabris was more obvious( P < 0. 05). Conclusion: Ginseng,Radix Astragali seu Hedysari and Radix et Caulis Acanthopanacis Senticosi extracts have certain synergistic effect on Mylabris extracts. The synergistic effect of the three extracts is more obvious when they are combined,which may be related to regulating the expression of caspase-3,a protein related to apoptosis of A549 cells.
Objective: To observe the effects of extracts of ginseng,Radix Astragali seu Hedysari and Radix et Caulis Acanthopanacis Senticosi on the growth inhibition and apoptosis of A549 human lung adenocarcinoma cells. Methods: The experiment was divided into Mylabris extract group,Mylabris extract + Ginseng group,Mylabris extract + Radix Astragali seu Hedysari group,Mylabris extract + Radix et Caulis Acanthopanacis Senticosi group,Mylabris extract + ginseng + Radix Astragali seu Hedysari + Radix et Caulis Acanthopanacis Senticosi group and control group( PBS buffer solution). The effects of three extracts on the growth inhibition of A549 human lung adenocarcinoma cells,the morphology of A549 cells,the apoptosis of A549 cells and the expression of Caspase-3 apoptosis-related proteins were detected. Results: MTT assay showed that the extracts of Ginseng,Radix Astragali seu Hedysari and Radix et Caulis Acanthopanacis Senticosi could enhance the inhibitory effect of Mylabris extracts on A549 cell proliferation,and the synergistic effect of three extracts on Mylabris extracts was more obvious( P < 0. 05). Microscopically,the extract of Mylabris could cause A549 cell suspension,growth retardation and activity decrease. After the intervention of Ginseng,stragali seu Hedysari and Radix et Caulis Acanthopanacis Senticosi,cell suspension was more obvious and the number of dead cells increased significantly( P < 0. 05). Flow pattern analysis showed that ginseng,Radix Astragali seu Hedysari and Radix et Caulis Acanthopanacis Senticosi extracts could enhance the apoptosis of A549 cells induced by Mylabris extracts,and the synergistic effect of the three extracts on Mylabris extracts was more obvious( P < 0. 05). Western blot analysis showed that the extracts of ginseng,Astragalu Radix Astragali seu Hedysari and Radix et Caulis Acanthopanacis Senticosi could regulate the expression of Caspase-3 protein in A549 cells induced by extracts of Mylabris to some extent,and the synergistic effect of the three extracts on Mylabris was more obvious( P < 0. 05). Conclusion: Ginseng,Radix Astragali seu Hedysari and Radix et Caulis Acanthopanacis Senticosi extracts have certain synergistic effect on Mylabris extracts. The synergistic effect of the three extracts is more obvious when they are combined,which may be related to regulating the expression of caspase-3,a protein related to apoptosis of A549 cells.
引文
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[4]JIANCHENG W,LONG G,YE Z.Effect of Aidiinjectionplus chemotherapy on gastric carcinoma:a Meta-analysis of randomized controlled trials[J].J Tradit Chin Med,2015,35(4):361-374.
[5]XIAO Z,WANG C,CHEN L,et al.Hasaidiinjectionthe attenuation and synergistic efficacy to gemcitabine and cisplatin in non-small cell lung cancer?A meta-analysis of 36 randomized controlled trials[J].Oncotarget,8(1):1329-1342.
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[11]SHEN K,FENG X,PAN H,et al.Baicalin Ameliorates Experimental Liver Cholestasis in Mice by Modulation of Oxidative Stress,Inflammation,and NRF2 Transcription Factor[J].Oxid Med Cell Longev,2017:6169128.
[12]TARHOUNI-JABBERI S,ZAKRAOUI O,IOANNOU E,et al.Mertensene,a Halogenated Monoterpene,Induces G2/M Cell Cycle Arrest and Caspase Dependent Apoptosis of Human Colon Adenocarcinoma HT29 Cell Line through the Modulation of ERK-1/-2,AKTand NF-κB Signaling[J].Mar Drugs,2017,15(7):E221.
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