叶下珠薄层鉴别方法研究
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摘要
目的:采用薄层色谱法,建立叶下珠的薄层鉴别方法,为其质量控制提供依据。方法:以甲醇为提取溶剂,叶下珠为对照药材,没食子酸为对照品,醋酸为展开剂在聚酰胺薄膜上展开,喷以1%三氯化铁乙醇溶液显色,对叶下珠进行薄层色谱鉴别研究。结果:供试品溶液在与对照药材和对照品相应位置上显相同颜色的清晰斑点。结论:本方法简便、快捷,重现性好,检出斑点清晰,可作为叶下珠的薄层鉴别方法。
Objective To establish a thin layer chromatography identification method of Phyllanthus urinaria Linnaeus,and to provide experimental basis for quality control standard. Method Methanol was used as extracting solvent.Phyllanthi urinariae herba was used as contrast material, gallic acid was used as the reference material. Phyllanthus urinaria Linnaeus was identified with acetic acid as mobile phase, polyamide film as stationary phase and chloride ferric( 1 %) as color developing reagent. Result The test solution showed clear spots of the same color at the corresponding positions with the contrast material and the reference material. Conclusion The method was simple, rapid, and reproducible, the spots were clear and easy to be identified. It can be used for TLC identification of Phyllanthus urinaria Linnaeus.
Objective To establish a thin layer chromatography identification method of Phyllanthus urinaria Linnaeus,and to provide experimental basis for quality control standard. Method Methanol was used as extracting solvent.Phyllanthi urinariae herba was used as contrast material, gallic acid was used as the reference material. Phyllanthus urinaria Linnaeus was identified with acetic acid as mobile phase, polyamide film as stationary phase and chloride ferric( 1 %) as color developing reagent. Result The test solution showed clear spots of the same color at the corresponding positions with the contrast material and the reference material. Conclusion The method was simple, rapid, and reproducible, the spots were clear and easy to be identified. It can be used for TLC identification of Phyllanthus urinaria Linnaeus.
引文
[1]刘亚梅.叶下珠复方联合拉米夫定治疗拉米夫定耐药性乙型肝炎20例[J].陕西中医, 2010, 31(1):15-17.
[2]杨琳媛,赵燕,吕卓. HPLC法测定不同产地叶下珠中没食子酸的含量[J].陕西中医. 2014, 35(10):1416-1417.
[3]冀德富,郭东艳,裴妙荣,崔春利.叶下珠的薄层鉴别及其多酚类成分的含量测定[J].中国实验方剂学杂志. 2009, 15(11):14-16.
[4]徐晓梅,谭本仁,朱兴保等.复方叶下珠滴丸质量标准及稳定性研究[J].中药新药与临床药理. 2014, 25(4):483-487.
[5]湖南省食品药品监督管理局.湖南省中药饮片炮制规范[S].湖南:湖南科学技术出版, 2009:377.
[6]河北省食品药品监督管理局.河北省中药材炮制规范[S].北京:学苑出版社, 2003:44.
[2]杨琳媛,赵燕,吕卓. HPLC法测定不同产地叶下珠中没食子酸的含量[J].陕西中医. 2014, 35(10):1416-1417.
[3]冀德富,郭东艳,裴妙荣,崔春利.叶下珠的薄层鉴别及其多酚类成分的含量测定[J].中国实验方剂学杂志. 2009, 15(11):14-16.
[4]徐晓梅,谭本仁,朱兴保等.复方叶下珠滴丸质量标准及稳定性研究[J].中药新药与临床药理. 2014, 25(4):483-487.
[5]湖南省食品药品监督管理局.湖南省中药饮片炮制规范[S].湖南:湖南科学技术出版, 2009:377.
[6]河北省食品药品监督管理局.河北省中药材炮制规范[S].北京:学苑出版社, 2003:44.