近红外荧光材料在医学影像诊断中的构建与应用
|
摘要
近红外荧光材料包括传统分子染料和新型量子点。近红外光谱属于分子振动光谱的倍频和主频吸收光谱,主要由分子振动的非谐振性使分子振动从基态向高能级跃迁时产生,具有较强的人体组织穿透能力。近年来通过生物分子修饰的近红外荧光材料成为医学影像诊断的研究热点,其具有组织穿透性强、背景荧光干扰低、荧光信号灵敏度高、光谱稳定抗淬灭、分子靶向识别、生物安全性良好等特点。文章分析近红外荧光材料的构建方法以及在超声波、计算机断层扫描(computed tomography,CT)、磁共振成像(magnetic resonance imaging,MRI)和正电子发射计算机断层显像(positron emission computed tomography,PET)等临床影像检验中的应用,探讨多模态造影策略的诊断优势和关键技术,为进一步临床应用提供理论依据。
Near-infrared fluorescent materials include traditional molecular dyes and new quantum dots. Near-infrared spectroscopy belongs to the frequency doubling and main frequency absorption spectroscopy of molecular vibration spectroscopy. It is mainly produced by the non-resonance of molecular vibration when molecular vibration transits from ground state to a higher energy level,and possesses strong tissue penetrability. In recent years,near-infrared fluorescent materials modified by molecular biological techniques have become a research hotspot in medical imaging diagnosis. They have strong tissue penetrability,low background fluorescence interference,high fluorescence signal sensitivity,spectral stability,quenching resistance,molecular targeting recognition and good biological safety. In this paper,the construction of near-infrared fluorescent material and its application in clinical imaging examinations such as ultrasonography,CT,MRI and PET are analyzed. The diagnostic advantages and key techniques of multimodal contrast strategy are discussed,which provide theoretical basis for further clinical application.
Near-infrared fluorescent materials include traditional molecular dyes and new quantum dots. Near-infrared spectroscopy belongs to the frequency doubling and main frequency absorption spectroscopy of molecular vibration spectroscopy. It is mainly produced by the non-resonance of molecular vibration when molecular vibration transits from ground state to a higher energy level,and possesses strong tissue penetrability. In recent years,near-infrared fluorescent materials modified by molecular biological techniques have become a research hotspot in medical imaging diagnosis. They have strong tissue penetrability,low background fluorescence interference,high fluorescence signal sensitivity,spectral stability,quenching resistance,molecular targeting recognition and good biological safety. In this paper,the construction of near-infrared fluorescent material and its application in clinical imaging examinations such as ultrasonography,CT,MRI and PET are analyzed. The diagnostic advantages and key techniques of multimodal contrast strategy are discussed,which provide theoretical basis for further clinical application.
引文
[1]赵勇,王浩,师长宏.近红外荧光(NIRF)染料在肿瘤诊断中的应用[J].中国比较医学杂志,2018,5(3):98-102
[2]Hilderbrand SA,Weissleder R.Near-infrared fluorescence:application to in vivo molecular imaging[J].Curr Opin Chem Biol,2010,14(1):71-79
[3]Luo G,Long J,Zhang B,et al.Quantum dots in cancer therapy[J].Expert Opin Drug Deliv,2012,9(1):47-58
[4]李卓平,李伟.量子点在前列腺癌中的研究进展[J].国际泌尿系统杂志,2018,38(3):490-493
[5]Feng HL,Li Q,Wang L,et al.Indocyanine green clearance test combined with MELD score in predicting the short-term prognosis of patients with acute liver failure[J].Hepatobiliary Pancreat Dis Int,2014,13(3):271-275
[6]陈玥,高卫平,白净.吲哚菁绿在影像医学中的应用[J].国际生物医学工程杂志,2013,36(5):289-293
[7]王金伟,张雅敏,刘子荣,等.荧光导航肝切除术中应用吲哚菁绿的可行性[J].山东医药,2016,56(19):86-88
[8]Tanaka T,Takatsuki M,Hidaka M,et al.Is a fluorescence navigation system with indocyanine green effective enough to detect liver malignancies?[J].J Hepatobiliary Pancreat Sci,2013,21(3):199-204
[9]Ishizawa T,Zuker NB,Kokudo N,et al.Positive and negative staining of hepatic segments by use of fluorescent imaging techniques during laparoscopic hepatectomy[J].Arch Surg,2012,147(4):393-394
[10]Zheng C,Zheng M,Gong P,et al.Indocyanine green-loaded biodegradable tumor targeting nanoprobes for in vitro and in vivo imaging[J].Biomaterials,2012,33(22):5603-5609
[11]牛诚诚,徐燕,刘明辉,等.近红外荧光/超声双模式纳米级造影剂显像淋巴结的实验研究[J].临床超声医学杂志,2016,18(8):505-508
[12]Zhang D,Tao L,Zhao H,et al.A functional drug delivery platform for targeting and imaging cancer cells based on Pluronic F127[J].J Biomater Sci Polym Ed,201,26(8):468-482
[13]Wu CX,Zhang YJ,Li Z,et al.A novel photoacoustic nanoprobe of ICG@PEG-Ag2S for atherosclerosis targeting and imaging in vivo[J].Nanoscale,2016,8(25):12531-12539
[14]Kweon S,Lee HJ,Hyung WJ,et al.Liposomes coloaded with iopamidol/lipiodol as a RES-targeted contrast agent for computed tomography imaging[J].Pharm Res,2010,27(7):1408-1415
[15]Mishra S.Collimator width optimization in X-ray luminescent computed tomography[J].J Med Imaging Health Inform,2013,4(5):641-686
[16]Carpenter CM,Sun C,Pratx G,et al.Hybrid x-ray/optical luminescence imaging:Characterization of experimental conditions[J].Medical Physics,2010,37(8):4011-4018
[17]Sudheendra L,Das GK,Li C,et al.NaGdF4:Eu(3+)nanoparticles for enhanced X-ray excited optical imaging[J].Chem Mater,2014,26(5):1881-1888
[18]Pires AM,Davolos MR,Stucchi EB.Eu3+as a spectroscopic probe in phosphors based on spherical fine particle gadolinium compounds[J].Int J Inorg Mater,2001,3(7):785-790
[19]Jiang C,Wu H,Song X,et al.Presence of photoluminescent carbon dots in Nescafe original instant coffee:applications to bioimaging[J].Talanta,2014,127:68-74
[20]Shi H,Wei J,Qiang L,et al.Fluorescent carbon dots for bioimaging and biosensing applications[J].J Biomed Nanotechnol,2014,10(10):2677-2699
[21]袁晨燕,王玲,安艳丽.荧光载体CS-Qdots的构建及生物相容性分析[J].南京医科大学学报(自然科学版),2017,37(10):1227-1233
[22]吕春祥,李利平.荧光碳点的制备及其肿瘤诊断和治疗中的应用研究进展[J].新型炭材料,2018,33(1):12-18
[23]Ding H,Yu SB,Wei JS,et al.Full-color light-emitting carbon dots with a surface-state-controlled luminescence mechanism[J].ACS Nano,2016,10(1):484-491
[24]Tan X,Li Y,Li X,et al.Electrochemical synthesis of smallsized red fluorescent graphene quantum dots as a bioimaging platform[J].Chem Commun(Camb),2015,51(13):2544-2546
[25]Yao H,Li S,Zeng M,et al.Construction of magnetic-carbon-quantum-dots-probe-labeled apoferritin nanocages for bioimaging and targeted therapy[J].Int J Nanomed,2016,11:4423-4438
[26]Yao H,Long X,Cao L,et al.Multifunctional ferritin nanocages for bimodal imaging and targeted delivery of doxorubicin into cancer cells[J].Rsc Advances,2016,6(111):109322-109333
[27]Yang X,Zhuo Y,Zhu S,et al.Novel and green synthesis of high-fluorescent carbon dots originated from honey for sensing and imaging[J].Biosens Bioelectron,2014,60(15):292-298
[28]师长宏.基于近红外荧光制剂的多模态多功能分子影像技术在肿瘤模型中的应用[J].中国实验动物学报,2018,26(2):234-238
[29]Xiao L,Zhang Y,Yue W,et al.Heptamethine cyanine based 64Cu-PET probe PC-1001 for cancer imaging:Synthesis and in vivo evaluation[J].Nucl Med Biol,2013,40(3):351-360
[30]Pan D,Wu JB,Chung LWK.Near-infrared fluorescence and nuclear imaging and targeting of prostate cancer[J].Transl Androl Urol,2013,2(3):254-264
[31]葛尚,朱昭环,蔡晓蔓,等.双源CT“双低”冠状动脉成像技术中对比剂用量的优化选择[J].南京医科大学学报(自然科学版),2018,38(11):78-82
[32]彭飞,施海彬,唐立钧,等.多排螺旋CT冠状动脉成像对比剂精准化的应用研究[J].南京医科大学学报(自然科学版),2016,36(2):222-2242018-11-19
[2]Hilderbrand SA,Weissleder R.Near-infrared fluorescence:application to in vivo molecular imaging[J].Curr Opin Chem Biol,2010,14(1):71-79
[3]Luo G,Long J,Zhang B,et al.Quantum dots in cancer therapy[J].Expert Opin Drug Deliv,2012,9(1):47-58
[4]李卓平,李伟.量子点在前列腺癌中的研究进展[J].国际泌尿系统杂志,2018,38(3):490-493
[5]Feng HL,Li Q,Wang L,et al.Indocyanine green clearance test combined with MELD score in predicting the short-term prognosis of patients with acute liver failure[J].Hepatobiliary Pancreat Dis Int,2014,13(3):271-275
[6]陈玥,高卫平,白净.吲哚菁绿在影像医学中的应用[J].国际生物医学工程杂志,2013,36(5):289-293
[7]王金伟,张雅敏,刘子荣,等.荧光导航肝切除术中应用吲哚菁绿的可行性[J].山东医药,2016,56(19):86-88
[8]Tanaka T,Takatsuki M,Hidaka M,et al.Is a fluorescence navigation system with indocyanine green effective enough to detect liver malignancies?[J].J Hepatobiliary Pancreat Sci,2013,21(3):199-204
[9]Ishizawa T,Zuker NB,Kokudo N,et al.Positive and negative staining of hepatic segments by use of fluorescent imaging techniques during laparoscopic hepatectomy[J].Arch Surg,2012,147(4):393-394
[10]Zheng C,Zheng M,Gong P,et al.Indocyanine green-loaded biodegradable tumor targeting nanoprobes for in vitro and in vivo imaging[J].Biomaterials,2012,33(22):5603-5609
[11]牛诚诚,徐燕,刘明辉,等.近红外荧光/超声双模式纳米级造影剂显像淋巴结的实验研究[J].临床超声医学杂志,2016,18(8):505-508
[12]Zhang D,Tao L,Zhao H,et al.A functional drug delivery platform for targeting and imaging cancer cells based on Pluronic F127[J].J Biomater Sci Polym Ed,201,26(8):468-482
[13]Wu CX,Zhang YJ,Li Z,et al.A novel photoacoustic nanoprobe of ICG@PEG-Ag2S for atherosclerosis targeting and imaging in vivo[J].Nanoscale,2016,8(25):12531-12539
[14]Kweon S,Lee HJ,Hyung WJ,et al.Liposomes coloaded with iopamidol/lipiodol as a RES-targeted contrast agent for computed tomography imaging[J].Pharm Res,2010,27(7):1408-1415
[15]Mishra S.Collimator width optimization in X-ray luminescent computed tomography[J].J Med Imaging Health Inform,2013,4(5):641-686
[16]Carpenter CM,Sun C,Pratx G,et al.Hybrid x-ray/optical luminescence imaging:Characterization of experimental conditions[J].Medical Physics,2010,37(8):4011-4018
[17]Sudheendra L,Das GK,Li C,et al.NaGdF4:Eu(3+)nanoparticles for enhanced X-ray excited optical imaging[J].Chem Mater,2014,26(5):1881-1888
[18]Pires AM,Davolos MR,Stucchi EB.Eu3+as a spectroscopic probe in phosphors based on spherical fine particle gadolinium compounds[J].Int J Inorg Mater,2001,3(7):785-790
[19]Jiang C,Wu H,Song X,et al.Presence of photoluminescent carbon dots in Nescafe original instant coffee:applications to bioimaging[J].Talanta,2014,127:68-74
[20]Shi H,Wei J,Qiang L,et al.Fluorescent carbon dots for bioimaging and biosensing applications[J].J Biomed Nanotechnol,2014,10(10):2677-2699
[21]袁晨燕,王玲,安艳丽.荧光载体CS-Qdots的构建及生物相容性分析[J].南京医科大学学报(自然科学版),2017,37(10):1227-1233
[22]吕春祥,李利平.荧光碳点的制备及其肿瘤诊断和治疗中的应用研究进展[J].新型炭材料,2018,33(1):12-18
[23]Ding H,Yu SB,Wei JS,et al.Full-color light-emitting carbon dots with a surface-state-controlled luminescence mechanism[J].ACS Nano,2016,10(1):484-491
[24]Tan X,Li Y,Li X,et al.Electrochemical synthesis of smallsized red fluorescent graphene quantum dots as a bioimaging platform[J].Chem Commun(Camb),2015,51(13):2544-2546
[25]Yao H,Li S,Zeng M,et al.Construction of magnetic-carbon-quantum-dots-probe-labeled apoferritin nanocages for bioimaging and targeted therapy[J].Int J Nanomed,2016,11:4423-4438
[26]Yao H,Long X,Cao L,et al.Multifunctional ferritin nanocages for bimodal imaging and targeted delivery of doxorubicin into cancer cells[J].Rsc Advances,2016,6(111):109322-109333
[27]Yang X,Zhuo Y,Zhu S,et al.Novel and green synthesis of high-fluorescent carbon dots originated from honey for sensing and imaging[J].Biosens Bioelectron,2014,60(15):292-298
[28]师长宏.基于近红外荧光制剂的多模态多功能分子影像技术在肿瘤模型中的应用[J].中国实验动物学报,2018,26(2):234-238
[29]Xiao L,Zhang Y,Yue W,et al.Heptamethine cyanine based 64Cu-PET probe PC-1001 for cancer imaging:Synthesis and in vivo evaluation[J].Nucl Med Biol,2013,40(3):351-360
[30]Pan D,Wu JB,Chung LWK.Near-infrared fluorescence and nuclear imaging and targeting of prostate cancer[J].Transl Androl Urol,2013,2(3):254-264
[31]葛尚,朱昭环,蔡晓蔓,等.双源CT“双低”冠状动脉成像技术中对比剂用量的优化选择[J].南京医科大学学报(自然科学版),2018,38(11):78-82
[32]彭飞,施海彬,唐立钧,等.多排螺旋CT冠状动脉成像对比剂精准化的应用研究[J].南京医科大学学报(自然科学版),2016,36(2):222-2242018-11-19