不同培养基浓度对土壤耐低温微生物分离效果的影响
|
摘要
从环境中分离筛选具有特定功能的纯培养微生物是微生物应用研究的基础,而提高环境微生物的可培养性是筛选纯培养微生物的关键。本研究以常规微生物分离培养基营养浓度为基础,对贫营养、富营养培养基在低温条件下(8℃)获得的培养物进行比较分析,了解培养基营养浓度对低温土壤微生物分离培养的影响,以期为特殊培养基的设计及多培养基组合提高土壤低温微生物的可培养性提供思路和依据。结果表明在低温培养条件下,稀释培养基营养成分可以提高耐低温可培养性微生物种类,但会降低耐低温可培养微生物的数量。通过比较低温条件下实验室分离培养方法和16 s RNA高通量测序法对常年低温环境土壤微生物多样性分析,通用常规方法无法分离和培养出土壤中的绝大部分微生物,且通过常规方法分离培养出来的微生物也不一定是土壤环境的优势菌群。
Improving the culturability of soil microorganism and isolating pure strains are the base of the study of applied microbiology. The cold-adapted microorganisms was isolated from the soil of Sayram Lake by using traditional nutrient medium and dilution of traditional nutrient medium and six traditional nutrient medium were used for this study. Results showed that the approach of dilute the traditional nutrient medium could increase the culturability of cold-adapted microorganism in the condition of 8 ℃. But the amount of the cold-adapted microorganism cultured on dilution of traditional nutrient medium was lower than those on traditional nutrient medium. The results of the 16 S rRNA sequencing showed that only a small proportion of cold-adapted microorganisms in the sample was recovered by culturing techniques.
Improving the culturability of soil microorganism and isolating pure strains are the base of the study of applied microbiology. The cold-adapted microorganisms was isolated from the soil of Sayram Lake by using traditional nutrient medium and dilution of traditional nutrient medium and six traditional nutrient medium were used for this study. Results showed that the approach of dilute the traditional nutrient medium could increase the culturability of cold-adapted microorganism in the condition of 8 ℃. But the amount of the cold-adapted microorganism cultured on dilution of traditional nutrient medium was lower than those on traditional nutrient medium. The results of the 16 S rRNA sequencing showed that only a small proportion of cold-adapted microorganisms in the sample was recovered by culturing techniques.
引文
[1] Alikunju, AP, Joy S, Salam JA, et al. Functional Characterization of a New Cold-Adaptedβ-Galactosidase from an Arctic Fjord Sediment Bacteria Enterobacter ludwigii MCC 3423[J]. Catalysis Letters, 2018,148(11):1–13
[2]张璐洁,屈建航,符运会,等.环磷酸腺苷对太湖界面沉积物可培养细菌多样性的影响[J].微生物学通报,2018,45(2):284-292
[3] Pandey A, Dhakar K, Jain R. Cold Adapted Fungi from Indian Himalaya:Untapped Source for Bioprospecting,Proceedings of the National Academy of Sciences[J]. India Section B:Biological Sciences, 2018,7(29):1–8
[4]王萍,崔中利,刘标,等.培养方法对土壤可培养细菌多样性的影响[J].土壤学报,2009,46(6):1096-1101
[5]郭斌,吴晓磊,钱易.提高微生物可培养性的方法和措施[J].微生物学报.2006,46(3):504-507
[6]袁志辉,王健,杨文蛟,等.土壤微生物分离新技术的研究进展[J].土壤学报,2014,51(6):1183-1188
[7]代先祝,梁晓丽,邱勤.低营养浓度培养基分离培养生物膜中的细菌及其鉴定[J].微生物学通报,2010,37(7):951-955
[8]周楠,姜成英,刘双江.从环境中分离培养微生物:培养基营养水平至关重要[J].微生物学通报,2016,43(5):1075-1081
[2]张璐洁,屈建航,符运会,等.环磷酸腺苷对太湖界面沉积物可培养细菌多样性的影响[J].微生物学通报,2018,45(2):284-292
[3] Pandey A, Dhakar K, Jain R. Cold Adapted Fungi from Indian Himalaya:Untapped Source for Bioprospecting,Proceedings of the National Academy of Sciences[J]. India Section B:Biological Sciences, 2018,7(29):1–8
[4]王萍,崔中利,刘标,等.培养方法对土壤可培养细菌多样性的影响[J].土壤学报,2009,46(6):1096-1101
[5]郭斌,吴晓磊,钱易.提高微生物可培养性的方法和措施[J].微生物学报.2006,46(3):504-507
[6]袁志辉,王健,杨文蛟,等.土壤微生物分离新技术的研究进展[J].土壤学报,2014,51(6):1183-1188
[7]代先祝,梁晓丽,邱勤.低营养浓度培养基分离培养生物膜中的细菌及其鉴定[J].微生物学通报,2010,37(7):951-955
[8]周楠,姜成英,刘双江.从环境中分离培养微生物:培养基营养水平至关重要[J].微生物学通报,2016,43(5):1075-1081