摘要
旨在建立肺炎支原体感染猪模型。选取姜曲海猪和杜洛克猪的杂交一代为试验动物,随机分成感染组和对照组,人工接种猪肺炎支原体,试验期40 d,期间观察猪群临床症状,称体质量,检测血清抗体和IgG水平,屠宰后检查肺组织病变,并采用qRT-PCR检测免疫相关分子基因的表达。结果显示,试验16 d后感染组出现支原体肺炎临床症状;试验30 d后,感染组的体质量极显著低于对照组;试验20 d后,感染组血清肺炎支原体抗体阳性,IgG质量浓度极显著高于对照组;试验40 d时屠宰,病理检查发现,感染组表现严重的肺组织病变,脾和肺组织的 IL-1β、 TLR4、 IL-4、 IL-2基因表达显著高于对照组。结果表明,感染组表现出典型支原体肺炎临床表现和病理变化,成功建立肺炎支原体感染猪模型。
In order to establish an animal model for Mycoplasma hyopneumonia(Mhp) infection, crossbred offspring of Jiangquhai pig and Duroc pig were used as experimental materials,the experimental pigs were divided into infection group and control group randomly and the infection group was inoculated with Mhp. The experiment lasted 40 days, clinical feature,body mass change, antibody and IgG production, pulmonary lesion, immunological molecules expression were detected for evaluating the impact of Mhp infection. The experiment results showed the infected pigs appeared clinical symptoms of Mhp at the 16 th-day post inoculation(dpi). Starting 30 th dpi, the average body mass of infected pigs was significantly lower than that of control pigs; starting 20 th dpi, the infected pigs were detected positive Mhp antibody titers in the serum and showed very significantly higher levels of IgG compared with control pigs. At the 40 th dpi, all experimental pigs were slaughtered and dissected; the infected pigs showed serious Mhp pulmonary lesions. In spleen and lung tissue of infected pigs, we observed significantly higher levels of IL-1β, TLR4, IL-4, IL-2 gene expression. The results showed that the infected pigs showed Mhp-typical clinical symptoms and pathologic changes, and successfully established a pig model of Mhp infection.
引文
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