HSP1A1基因+190G>C多态与有氧运动能力表型指标的关联性研究
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摘要
目的:探讨热休克蛋白70(Heat Shock Protein 70,HSP70)基因(HSPA1A)+190G>C单核苷酸多态性(Single Nucleotide Polymorphism,SNP)与有氧运动能力表型指标的关系。方法:试验对象为400名大学一年级学生,其中男生300名,女生100名,均为汉族。受试者以95%~105%个体通气无氧阈(VT)进行每周3次,每次5 000 m,为期18天的长跑训练。测定训练前后摄氧量绝对值(VO2)、摄氧量相对值(rVO2)、通气无氧阈(VT)和每分钟通气量(VE),并进行跑节省化试验。采用ABI7900HT的Taqman基因分型技术,检测HSP1A1基因+190G>C位点基因型。结果:HSP1A1+190G>C位点基因多态性与有氧运动能力表型存在关联,随着G等位基因的增加,个体有氧运动能力也在增加;有氧耐力训练前后,不同基因型者VO2max、rVO2max、VT/VO2和VE均无统计学差异(P>0.05);有氧耐力训练前GG基因型个体RE/HR值显著低于GC和CC基因型者,且随着C基因型的增加,RE/HR值也逐渐增加,趋势检验发现具有剂量效应关系(Ptrend<0.05);有氧耐力训练后不同基因型RE/HR和RE/VE值以及他们的变化率间差异具有统计学意义(P<0.05),且随着C基因型的增加,RE/HR和RE/VE值以及他们的变化率也逐渐增加,趋势检验发现具有剂量效应关系(Ptrend<0.05)。结论:HSPA1A基因+190G>C位点与有氧耐力训练后有氧运动能力表型存在关联,GG基因型个体具有较高的有氧运动能力水平。
Objective:To explore the associations between the heat shock protein 70 gene(HSPA1A)+190G>C polymorphisms and the aerobic capacity to aerobic endurance training program among healthy freshmen.Methods:A total of 400 healthy freshmen were included in the present study,including 300 boys and 100 girls and all were Han nationality.All subjects performed an aerobic endurance training program of 18-week 5000m running,3 times a week with the intensity of 95%-105% aerobic threshold.VO2max,rVO2max,VE,VT and VE were measured before and after the aerobic endurance training.Polymorphisms of HSP1A1 +190G>C were detected by Taqman single nucleotide polymorphism(SNP)allelic discrimination by means of an ABI7900HT.Results:The +190G>C SNP in HSPA1A gene was associated with aerobic capacity in men and women,individual aerobic capacity is also increasing with the increase of the G allele.People with the HSPA1A GG genotype have lower RE/HR values,than carriers of the HSPA1A C190 allele after the endurance training(P<0.05),and there was a dose response relationship among the three genotype groups(Ptrend<0.05).After the endurance training the RE/HR and RE/VE values were significant reduced,and these values in GG genotype were significant lower than the C allele,and there were also dose response relationships among the three genotype groups(Ptrend<0.05).Conclusion:The +190G>C SNP in HSPA1A gene was not only associated with aerobic capacity but also with endurance training promotion.
Objective:To explore the associations between the heat shock protein 70 gene(HSPA1A)+190G>C polymorphisms and the aerobic capacity to aerobic endurance training program among healthy freshmen.Methods:A total of 400 healthy freshmen were included in the present study,including 300 boys and 100 girls and all were Han nationality.All subjects performed an aerobic endurance training program of 18-week 5000m running,3 times a week with the intensity of 95%-105% aerobic threshold.VO2max,rVO2max,VE,VT and VE were measured before and after the aerobic endurance training.Polymorphisms of HSP1A1 +190G>C were detected by Taqman single nucleotide polymorphism(SNP)allelic discrimination by means of an ABI7900HT.Results:The +190G>C SNP in HSPA1A gene was associated with aerobic capacity in men and women,individual aerobic capacity is also increasing with the increase of the G allele.People with the HSPA1A GG genotype have lower RE/HR values,than carriers of the HSPA1A C190 allele after the endurance training(P<0.05),and there was a dose response relationship among the three genotype groups(Ptrend<0.05).After the endurance training the RE/HR and RE/VE values were significant reduced,and these values in GG genotype were significant lower than the C allele,and there were also dose response relationships among the three genotype groups(Ptrend<0.05).Conclusion:The +190G>C SNP in HSPA1A gene was not only associated with aerobic capacity but also with endurance training promotion.
引文
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[17]ZHANG X,TANGUAY R M,HE M,et al.Variants of HSPA1A inCombination with Plasma Hsp70 and anti-Hsp70 Antibody LevelsAssociated with Higher Risk of Acute Coronary Syndrome[J].Cardiology,2011,119(1):57-64.
[2]陈艺洁.ANP基因多态性与有氧运动能力的关联性研究[D].南京:南京体育学院,2009.
[3]CIOCCA D R,CALDERWOOD S K.Heat Shock Proteins in Cancer:Diagnostic,Prognostic,Predictive,and Treatment Implications[J].CellStress Chaperones,2005,10(2):86-103.
[4]吴顺华,成军,郑玉建.热休克蛋白家族与肝癌的关系[J].世界华人消化杂志,2005,13(14):1725-1730.
[5]朱洪竹,肖国强.预热适应对大鼠离心运动骨骼肌形态结构及HSP70表达的影响[J].体育科学,2011,31(12):73-78.
[6]石真玉,肖国强.热休克蛋白70与运动[J].中国体育科技,2006,42(5):136-139.
[7]王水泓,杨萍.机体运动中热激蛋白72的双重保护作用[J].中国运动医学杂志,2008,27(1):126-128.
[8]陆阿明,陆爱云,夏春林.有氧运动对大鼠中枢神经系统HSP70表达的影响[J].中国运动医学杂志,2005,24(4):411-414.
[9]DEMIREL H A,POWERS S K,CAILLAUD C,et al.Exercise TrainingReduces Myocardial Lipid Peroxidation Following Short-termIschemia-reperfusion[J].MedSciSportsExe,1998,30(8):1211-1216.
[10]LIU Y,MAYR S,OPITZ-GRESS A,et al.Human Skeletal MuscleHSP70ResponsetoTraininginHighlyTrainedRowers[J].JApplPhysiol,1999,86(1):101-104.
[11]FEHRENBACH E,PASSEK F,NIESS AM,et al.HSP Expression inHuman Leukocytes is Modulated by Endurance Exercise[J].Med SciSports Exe,2000,32(3):592-600.
[12]HE M,GUO H,YANG X,et al.Functional SNPs in HSPA1A GenePredictriskofCoronaryHeartDisease[J].PLoSOne,2009,4(3):e4851.
[13]席翼.ACE基因多态性及与有氧运动能力的关联研[D].北京:北京体育大学,2004.
[14]何耀锋.OB_R基因多态性与有氧运动能力的关联性研究[D].北京:北京体育大学,2007.
[15]席翼,张得保,王国军,等.跑节省化评价有氧耐力及其训练效果试验研究[J].中国运动医学杂志,2008,27(1):15-19.
[16]KRABENBUHL G,WILLIAMS T.Running Ecnnomy Changes with AgeDuring Hildhood and Adolescence[J].Med Sci Sports Exerc,1992,24(4):462-466.
[17]ZHANG X,TANGUAY R M,HE M,et al.Variants of HSPA1A inCombination with Plasma Hsp70 and anti-Hsp70 Antibody LevelsAssociated with Higher Risk of Acute Coronary Syndrome[J].Cardiology,2011,119(1):57-64.